##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921530_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1834752 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.947172151876657 31.0 30.0 34.0 26.0 34.0 2 29.981250327019673 31.0 30.0 34.0 25.0 34.0 3 30.087758999581414 31.0 30.0 34.0 26.0 34.0 4 33.4778613131366 37.0 35.0 37.0 27.0 37.0 5 33.51806347669876 37.0 35.0 37.0 27.0 37.0 6 33.57439806578764 37.0 35.0 37.0 28.0 37.0 7 33.389786194537464 37.0 35.0 37.0 27.0 37.0 8 33.33997183270546 37.0 35.0 37.0 27.0 37.0 9 34.805559552462675 39.0 35.0 39.0 27.0 39.0 10-11 34.687476291073665 39.0 35.0 39.0 27.0 39.0 12-13 34.5519895876936 39.0 35.0 39.0 25.5 39.0 14-15 35.70049167407912 40.0 36.0 41.0 25.5 41.0 16-17 35.630469404039346 40.0 36.0 41.0 25.0 41.0 18-19 35.64969652574299 40.0 36.0 41.0 25.0 41.0 20-21 35.692451077856845 40.0 36.0 41.0 25.0 41.0 22-23 35.65866940055113 40.0 36.0 41.0 25.0 41.0 24-25 35.628506195932744 40.0 36.0 41.0 25.0 41.0 26-27 35.55526932250244 40.0 35.0 41.0 25.0 41.0 28-29 35.515616824508164 40.0 35.0 41.0 25.0 41.0 30-31 35.416556025010465 40.0 35.0 41.0 25.0 41.0 32-33 35.31571514842333 40.0 35.0 41.0 25.0 41.0 34-35 35.13792027478373 40.0 35.0 41.0 24.5 41.0 36-37 34.98506419396191 39.5 35.0 41.0 24.0 41.0 38-39 34.856631849972096 39.0 34.5 41.0 23.5 41.0 40-41 34.7017308061253 39.0 34.0 41.0 23.0 41.0 42-43 34.49441068874704 39.0 34.0 41.0 21.5 41.0 44-45 34.29029182145598 39.0 33.5 41.0 20.5 41.0 46-47 34.0335756549114 38.5 33.0 41.0 19.0 41.0 48-49 33.876709495343235 38.0 33.0 40.5 17.5 41.0 50-51 33.67214479123064 38.0 33.0 40.0 15.0 41.0 52-53 33.480752439566764 38.0 33.0 40.0 14.5 41.0 54-55 33.27319904815474 38.0 33.0 40.0 13.0 41.0 56-57 33.02848395859495 37.0 32.5 40.0 12.0 41.0 58-59 32.779073139040044 37.0 32.0 40.0 9.5 41.0 60-61 32.472108492046885 36.0 32.0 40.0 8.0 41.0 62-63 32.160562571944325 36.0 31.5 39.0 7.0 41.0 64-65 31.85638331502023 35.0 31.0 39.0 6.5 41.0 66-67 31.812301880581135 35.0 31.5 39.0 2.0 41.0 68-69 31.569008509051905 35.0 31.0 38.5 2.0 40.0 70-71 31.269377005720663 35.0 31.0 37.0 2.0 40.0 72-73 30.945223932171757 35.0 31.0 37.0 2.0 39.0 74-75 30.64614154937561 35.0 31.0 36.0 2.0 39.0 76-77 30.37456370125227 35.0 31.0 36.0 2.0 38.5 78-79 30.106273490913214 35.0 30.5 35.5 2.0 37.0 80-81 29.866494763324962 35.0 30.0 35.0 2.0 37.0 82-83 29.66642739727222 35.0 30.0 35.0 2.0 36.5 84-85 29.465874407004325 35.0 30.0 35.0 2.0 36.0 86-87 29.313061656289243 34.5 30.0 35.0 2.0 36.0 88-89 29.183606013237757 34.0 30.0 35.0 2.0 35.5 90-91 29.07467848515767 34.0 30.0 35.0 2.0 35.0 92-93 28.979759117308497 34.0 30.0 35.0 2.0 35.0 94-95 28.911390749267476 34.0 30.0 35.0 2.0 35.0 96-97 28.8117650232838 34.0 29.0 35.0 2.0 35.0 98-99 28.7435468117762 34.0 29.0 35.0 2.0 35.0 100-101 28.674232539329566 34.0 29.0 35.0 2.0 35.0 102-103 27.67914137714525 32.5 27.5 34.5 2.0 35.0 104-105 28.513666697188505 34.0 29.0 35.0 2.0 35.0 106-107 28.84607156716548 34.5 30.0 35.0 2.0 35.0 108-109 28.884135022062928 35.0 30.0 35.0 2.0 35.0 110-111 28.822800574682574 35.0 30.0 35.0 2.0 35.0 112-113 28.74348822075136 35.0 30.0 35.0 2.0 35.0 114-115 28.622665897080367 35.0 30.0 35.0 2.0 35.0 116-117 28.558570586106462 35.0 30.0 35.0 2.0 35.0 118-119 28.401197273527977 35.0 30.0 35.0 2.0 35.0 120-121 28.23048537350007 34.5 29.5 35.0 2.0 35.0 122-123 28.098680094094462 34.0 29.0 35.0 2.0 35.0 124-125 27.239482502267336 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65533.0 3 11920.0 4 7867.0 5 6897.0 6 7304.0 7 8524.0 8 9377.0 9 9829.0 10 10389.0 11 11064.0 12 11035.0 13 11801.0 14 13267.0 15 11720.0 16 8237.0 17 7204.0 18 6487.0 19 6151.0 20 5923.0 21 6166.0 22 7274.0 23 9963.0 24 14265.0 25 19665.0 26 23425.0 27 24734.0 28 29451.0 29 35060.0 30 39498.0 31 47232.0 32 59846.0 33 77092.0 34 108574.0 35 172713.0 36 325798.0 37 470196.0 38 133091.0 39 180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.374036944895614 17.456166033398816 12.286377735141922 24.883419286563655 2 16.79253645662685 19.739595911907685 37.97496923899705 25.492898392468422 3 18.70404211334316 24.534424256169153 29.222183212232757 27.539350418254934 4 13.264479435967946 15.411384227209139 35.92580396484177 35.39833237198115 5 14.30061401203599 37.32868235481413 33.472924479027526 14.897779154122365 6 32.9805506964408 35.02848580290267 17.519859359527477 14.471104141129048 7 29.430431511106747 31.50380039476123 20.949717015081625 18.11605107905039 8 27.907217658370676 32.92314100820076 19.86059640183 19.309044931598567 9 27.90472877785386 14.288338996061533 19.079105187175337 38.72782703890927 10-11 26.239319641173747 24.74293805487296 27.161520338958102 21.856221964995193 12-13 26.468347977329294 22.715822876257096 27.094082335333425 23.721746811080184 14-15 24.080749053215143 23.826728284468366 24.504689488370875 27.587833173945615 16-17 24.172222622061994 26.67777312509678 24.998636909863755 24.15136734297747 18-19 24.221305072494786 25.826095942271373 26.48493869223368 23.467660293000165 20-21 25.448747803570786 24.810515374186462 26.049786819570496 23.690950002672253 22-23 25.196332116340514 24.469517465528384 25.85628941707044 24.477861001060667 24-25 24.587408842175993 24.959552827293503 25.984332702776474 24.468705627754026 26-27 24.258339522471207 24.954876549874637 26.148289562223464 24.638494365430695 28-29 24.363720935051507 24.92009277771764 26.163708992933156 24.55247729429769 30-31 24.707406302672457 24.861834919516625 25.943407801153523 24.487350976657396 32-33 24.3681620419502 25.027867184236445 25.853848343620378 24.750122430192974 34-35 24.728653217011995 25.028544165757904 25.980916030534353 24.261886586695745 36-37 24.849235064605754 24.81079061955541 25.741800563306548 24.59817375253229 38-39 24.77770194158529 25.079098542481702 25.674965594213226 24.46823392171978 40-41 24.772690836635135 24.930828433794332 25.90945477428312 24.387025955287413 42-43 24.677989117467813 24.96825360035666 25.819659726586945 24.53409755558858 44-45 24.6097502516135 24.926738273692774 25.719194694997533 24.744316779696188 46-47 24.668490057701327 25.118729480033597 25.64123190697979 24.57154855528529 48-49 24.913569623621612 25.264416968491354 25.23946939928435 24.582544008602685 50-51 24.70301398981404 25.39921916175632 25.253374348381243 24.644392500048397 52-53 24.829485201145154 25.194906193341815 25.456789874340007 24.51881873117302 54-55 24.713515211299402 25.223748505207848 25.477256529508413 24.58547975398434 56-57 24.69589223801011 25.241441810363042 25.278929599567707 24.783736352059144 58-59 24.780646149836226 25.063048384625407 25.34168816446181 24.814617301076552 60-61 24.713424379993725 25.2814641615886 25.50609572085058 24.499015737567092 62-63 24.5691041838198 25.377050878694853 25.39160945926051 24.662235478224833 64-65 24.61791296330072 25.07500789951436 25.488590756627616 24.818488380557298 66-67 24.54208180248663 25.290110046489033 25.4194385930668 24.748369557957535 68-69 24.721516257867645 25.21702554311247 25.34190467804709 24.7195535209728 70-71 24.53644743159691 25.019910260007137 25.6389618909035 24.804680417492456 72-73 24.888790144726837 25.261252518203303 25.36906842152611 24.480888915543755 74-75 24.540557192825577 25.49452976120697 25.319503929902424 24.645409116065025 76-77 24.61213556608907 25.17703061806421 25.390099806667514 24.820734009179205 78-79 24.63599888557457 25.25792896791883 25.364410027866086 24.74166211864051 80-81 24.558451449421927 25.40504927538444 25.254772573261597 24.781726701932037 82-83 24.757829733567053 25.332758395585696 25.24610888074394 24.66330299010331 84-85 24.544443566169956 25.317200945265633 25.21408849190882 24.924266996655593 86-87 24.386628773001338 25.373828349545896 25.32615690474133 24.913385972711435 88-89 24.53682805722921 25.271764610959558 25.311997767005323 24.87940956480591 90-91 24.440056417489423 25.187329231482046 25.411472918923707 24.96114143210482 92-93 24.51977354931735 25.41761725255466 25.274272989187168 24.788336208940823 94-95 24.427802577648063 25.144606350741334 25.440305964197695 24.987285107412905 96-97 24.54356084815142 25.15324565173725 25.338440476965935 24.9647530231454 98-99 24.412838700780085 25.203878668684965 25.22573692579149 25.157545704743463 100-101 24.35768709574379 25.357882239817897 25.327084362200488 24.957346302237834 102-103 24.529658393218448 25.08835597920882 25.504163250889555 24.877822376683177 104-105 24.259117339897397 25.317801460344175 25.402695684593564 25.02038551516486 106-107 24.307106969062275 25.386541006147205 25.292513651000437 25.013838373790083 108-109 24.34913765730712 25.234878988023222 25.33138985183225 25.08459350283741 110-111 24.334125951311908 25.406871929740888 25.34789570146691 24.91110641748029 112-113 24.289764636208016 25.393038372973077 25.448336967669267 24.868860023149644 114-115 24.069064890941778 25.585749649718043 25.491369068951787 24.85381639038839 116-117 24.157687220665576 25.683354986478022 25.33709544798844 24.82186234486796 118-119 24.29143637882348 25.24257046848974 25.345220073870568 25.12077307881621 120-121 23.94897142974342 25.445207867588245 25.549370688653255 25.05645001401508 122-123 24.03668914487404 25.53062035599854 25.4849333858897 24.947757113237717 124-125 23.985163625264352 25.59612575490004 25.448715852355726 24.96999476747989 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 124.0 1 174.5 2 238.5 3 255.0 4 245.0 5 201.5 6 180.0 7 245.5 8 329.5 9 369.0 10 385.5 11 407.0 12 457.5 13 538.5 14 629.0 15 711.5 16 808.0 17 916.0 18 1051.5 19 1186.0 20 1301.5 21 1469.0 22 1709.5 23 2129.0 24 2621.0 25 3400.0 26 4801.5 27 6309.0 28 7337.5 29 8751.5 30 11156.5 31 13339.0 32 16044.5 33 18981.0 34 22061.0 35 25478.5 36 28893.5 37 32145.5 38 34007.5 39 36241.0 40 38837.0 41 40820.0 42 43396.0 43 48896.0 44 53674.5 45 56264.5 46 60169.0 47 63877.0 48 68842.5 49 74388.0 50 74984.5 51 73812.5 52 73424.5 53 74213.5 54 75616.0 55 75466.0 56 72094.0 57 65819.0 58 62153.0 59 58753.0 60 53301.0 61 47357.5 62 41162.0 63 32878.0 64 24875.5 65 19741.0 66 14991.5 67 11159.5 68 8913.5 69 7319.0 70 6298.0 71 5352.0 72 4357.0 73 3996.0 74 3318.0 75 2450.0 76 1694.5 77 966.0 78 565.0 79 358.0 80 204.0 81 102.0 82 68.0 83 36.0 84 29.0 85 25.0 86 16.5 87 14.0 88 12.0 89 9.0 90 8.5 91 8.5 92 7.5 93 6.5 94 6.0 95 7.5 96 7.5 97 8.5 98 8.0 99 7.5 100 21.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03351951653411469 2 0.024471972233849587 3 0.025289521417608485 4 0.02888673782614762 5 0.03193892144551416 6 0.04665480675317427 7 0.03989640016743407 8 0.035590641132970564 9 0.02921375749965118 10-11 0.03602666736430864 12-13 0.03635368703781219 14-15 0.03542713129621878 16-17 0.03728024277940561 18-19 0.05071530103251011 20-21 0.059626587135482065 22-23 0.05433976908050789 24-25 0.06472264371424585 26-27 0.04872593135203014 28-29 0.049407222338495885 30-31 0.048780434630947396 32-33 0.05744645597879169 34-35 0.040986465745779264 36-37 0.051069572345472304 38-39 0.061125427305706706 40-41 0.049379970699037254 42-43 0.05883628959118181 44-45 0.05921781254360262 46-47 0.06387784289102832 48-49 0.049379970699037254 50-51 0.05196887644760709 52-53 0.060689401074368626 54-55 0.0482354018417748 56-57 0.044774443630528814 58-59 0.0459462641272499 60-61 0.03474584030975303 62-63 0.04262156411329706 64-65 0.042430802637086644 66-67 0.036162925561601784 68-69 0.031530146853634713 70-71 0.04897119610715781 72-73 0.03308349030277661 74-75 0.04016891656202037 76-77 0.033028987023859355 78-79 0.03395554276545277 80-81 0.025562037812194783 82-83 0.01910339926049951 84-85 0.018912637784289102 86-87 0.017468300892981722 88-89 0.02419945583926329 90-91 0.011690953327752197 92-93 0.0194576705734617 94-95 0.01575144760708804 96-97 0.010764397586158783 98-99 0.01117317217803823 100-101 0.01144568857262453 102-103 0.006976419701409237 104-105 0.006322380354402121 106-107 0.009783338565648109 108-109 0.013271548416352728 110-111 0.006240625436026231 112-113 0.009074795939723733 114-115 0.008638769708385657 116-117 0.00793022708246128 118-119 0.23180244523510535 120-121 0.3468861186688991 122-123 0.028941241105064883 124-125 0.003924236082042696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1834752.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.65289610653075 #Duplication Level Percentage of deduplicated Percentage of total 1 79.314314207341 43.34756974134544 2 10.623716374114768 11.612337345194879 3 3.5848457726606364 5.877666107134732 4 1.7686017128677542 3.866368226687748 5 1.0108400233450041 2.7622667388098816 6 0.6938550705044125 2.275271344876034 7 0.4854080341472901 1.8570268401669026 8 0.3672292028294169 1.6056111575616185 9 0.285453706064116 1.4040784566671667 >10 1.720911990847576 17.055361950075675 >50 0.10285070483570594 3.8210279566077894 >100 0.03996048749441303 3.675779618945052 >500 0.0017108060056863568 0.5908131776555056 >1k 3.019069421799453E-4 0.24882133827159814 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.450327891725966E-5 5.450327891725966E-5 7 0.0 0.0 0.0 5.450327891725966E-5 5.450327891725966E-5 8 0.0 0.0 0.0 5.450327891725966E-5 5.450327891725966E-5 9 0.0 0.0 0.0 5.450327891725966E-5 5.450327891725966E-5 10-11 0.0 0.0 0.0 5.450327891725966E-5 5.450327891725966E-5 12-13 0.0 0.0 0.0 5.450327891725966E-5 1.63509836751779E-4 14-15 0.0 0.0 0.0 5.450327891725966E-5 2.1801311566903864E-4 16-17 0.0 0.0 0.0 5.450327891725966E-5 2.725163945862983E-4 18-19 0.0 0.0 0.0 5.450327891725966E-5 2.725163945862983E-4 20-21 0.0 0.0 0.0 5.450327891725966E-5 2.725163945862983E-4 22-23 0.0 0.0 0.0 5.450327891725966E-5 2.997680340449282E-4 24-25 0.0 0.0 0.0 1.63509836751779E-4 3.27019673503558E-4 26-27 0.0 0.0 0.0 1.9076147621040882E-4 3.27019673503558E-4 28-29 0.0 0.0 0.0 9.81059020510674E-4 3.27019673503558E-4 30-31 0.0 0.0 0.0 0.004551023789591182 3.542713129621878E-4 32-33 0.0 0.0 0.0 0.010927907422910561 3.8152295242081764E-4 34-35 0.0 5.450327891725966E-5 0.0 0.020220716478303334 3.8152295242081764E-4 36-37 0.0 5.450327891725966E-5 0.0 0.034745840309753036 3.8152295242081764E-4 38-39 0.0 5.450327891725966E-5 0.0 0.05692867482907772 3.8152295242081764E-4 40-41 0.0 5.450327891725966E-5 0.0 0.08216369296776894 3.8152295242081764E-4 42-43 0.0 5.450327891725966E-5 0.0 0.10704443979349798 4.0877459187944746E-4 44-45 0.0 5.450327891725966E-5 0.0 0.1371847530347426 4.360262313380773E-4 46-47 0.0 5.450327891725966E-5 0.0 0.1720941031812474 4.360262313380773E-4 48-49 0.0 5.450327891725966E-5 0.0 0.2090200746476908 4.360262313380773E-4 50-51 0.0 5.450327891725966E-5 0.0 0.25098759941398074 4.360262313380773E-4 52-53 0.0 5.450327891725966E-5 0.0 0.29257360122784987 4.632778707967071E-4 54-55 0.0 5.450327891725966E-5 0.0 0.33775681945025815 5.450327891725966E-4 56-57 0.0 5.450327891725966E-5 0.0 0.3883903655643923 5.450327891725966E-4 58-59 0.0 5.450327891725966E-5 0.0 0.4457550666248081 5.722844286312264E-4 60-61 0.0 5.450327891725966E-5 0.0 0.5075617849169807 5.995360680898563E-4 62-63 0.0 5.450327891725966E-5 0.0 0.5695047614064462 5.995360680898563E-4 64-65 2.725163945862983E-5 5.450327891725966E-5 0.0 0.636080516603879 5.995360680898563E-4 66-67 5.450327891725966E-5 5.450327891725966E-5 0.0 0.7131754046323426 5.995360680898563E-4 68-69 5.450327891725966E-5 5.450327891725966E-5 0.0 0.7969196926887121 5.995360680898563E-4 70-71 5.450327891725966E-5 5.450327891725966E-5 0.0 0.8933087414538858 7.085426259243756E-4 72-73 5.450327891725966E-5 5.450327891725966E-5 0.0 0.9941670590902749 7.630459048416353E-4 74-75 5.450327891725966E-5 5.450327891725966E-5 0.0 1.0945893504953257 7.630459048416353E-4 76-77 5.450327891725966E-5 5.450327891725966E-5 0.0 1.204413457513604 7.630459048416353E-4 78-79 5.450327891725966E-5 5.450327891725966E-5 0.0 1.330724806404353 7.630459048416353E-4 80-81 5.450327891725966E-5 5.450327891725966E-5 0.0 1.477611143086368 7.630459048416353E-4 82-83 1.0900655783451932E-4 5.450327891725966E-5 0.0 1.628694232245012 7.630459048416353E-4 84-85 1.0900655783451932E-4 5.450327891725966E-5 0.0 1.7856636755267197 7.630459048416353E-4 86-87 1.0900655783451932E-4 5.450327891725966E-5 0.0 1.9549508598437282 7.630459048416353E-4 88-89 1.0900655783451932E-4 5.450327891725966E-5 0.0 2.1470476663876097 7.630459048416353E-4 90-91 1.0900655783451932E-4 5.450327891725966E-5 0.0 2.356776283661225 7.630459048416353E-4 92-93 1.0900655783451932E-4 5.450327891725966E-5 0.0 2.586098829705595 8.175491837588949E-4 94-95 1.0900655783451932E-4 5.450327891725966E-5 0.0 2.82307908643784 8.175491837588949E-4 96-97 1.0900655783451932E-4 5.450327891725966E-5 0.0 3.070169701409237 8.175491837588949E-4 98-99 1.0900655783451932E-4 5.450327891725966E-5 0.0 3.3470463583089156 8.175491837588949E-4 100-101 1.0900655783451932E-4 5.450327891725966E-5 0.0 3.6653182555462536 8.175491837588949E-4 102-103 1.0900655783451932E-4 5.450327891725966E-5 0.0 4.002175770894377 8.720524626761547E-4 104-105 1.0900655783451932E-4 5.450327891725966E-5 0.0 4.340096100181387 9.265557415934143E-4 106-107 1.0900655783451932E-4 5.450327891725966E-5 0.0 4.703060686130877 9.265557415934143E-4 108-109 1.0900655783451932E-4 5.450327891725966E-5 0.0 5.087990093484024 9.265557415934143E-4 110-111 1.0900655783451932E-4 5.450327891725966E-5 0.0 5.503713853425422 9.265557415934143E-4 112-113 1.0900655783451932E-4 5.450327891725966E-5 0.0 5.947425047090833 9.265557415934143E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2085 0.0 71.36695 1 GTATCAA 4140 0.0 60.23888 1 TCAACGC 5235 0.0 46.936104 4 CAACGCA 5360 0.0 46.173244 5 AACGCAG 5645 0.0 43.94388 6 ATCAACG 5710 0.0 42.92859 3 TATCAAC 6065 0.0 40.317772 2 ACGCAGA 6615 0.0 36.95953 7 GTACATG 9400 0.0 36.85177 1 TACATGG 9670 0.0 35.685112 2 ACATGGG 10010 0.0 34.47303 3 CGCAGAG 7130 0.0 34.37056 8 CATGGGG 7955 0.0 32.83205 4 GCAGAGT 7535 0.0 31.180346 9 CCCTATA 310 0.0 28.788237 2 GTATAGA 545 0.0 28.39488 1 AGAGTAC 6915 0.0 26.966114 10-11 CAGAGTA 7220 0.0 26.733175 10-11 ATGGGGG 4075 0.0 26.716854 5 ATGGGGC 3105 0.0 22.609045 5 >>END_MODULE