FastQCFastQC Report
Mon 15 Aug 2016
SRR2921530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834752
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.18204095158364728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.17124930235802985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.080.882951
GTATCAA57050.058.0052871
TCAACGC76700.042.7800374
CAACGCA79950.040.934145
ATCAACG82450.040.0132643
AACGCAG82300.039.75886
GTACATG92300.038.0490531
TATCAAC86400.038.046112
TACATGG97350.035.2959372
ACGCAGA95500.034.2010467
ACATGGG100350.033.825363
CGCAGAG100150.032.7301038
CATGGGG78700.031.2507344
GTGGTAT10550.029.9539181
GCAGAGT107300.029.8283549
AGAGTAC100350.027.47761710-11
CAGAGTA103850.026.40834410-11
ATGGGGG40350.026.249315
GAGTACT60450.024.99746712-13
AGTACTT64500.024.71915612-13
Produced by FastQC (version 0.11.5)