##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921530_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1834752
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26875348820985	31.0	31.0	34.0	28.0	34.0
2	31.339535261441327	33.0	31.0	34.0	28.0	34.0
3	31.430577538544718	33.0	31.0	34.0	28.0	34.0
4	34.94671650446491	37.0	35.0	37.0	32.0	37.0
5	34.60300029649784	37.0	35.0	37.0	31.0	37.0
6	34.573569615948095	37.0	35.0	37.0	31.0	37.0
7	34.42144551416213	37.0	35.0	37.0	30.0	37.0
8	34.38393254238175	37.0	35.0	37.0	30.0	37.0
9	35.96456850844147	39.0	37.0	39.0	30.0	39.0
10-11	35.838519320322305	39.0	37.0	39.0	30.0	39.0
12-13	35.744378259296084	39.0	37.0	39.0	30.0	39.0
14-15	36.92421646086228	40.0	37.0	41.0	30.0	41.0
16-17	36.9016240342019	40.0	37.0	41.0	30.0	41.0
18-19	36.92666393060206	40.0	37.0	41.0	30.0	41.0
20-21	36.91483399391307	40.0	37.0	41.0	30.0	41.0
22-23	36.84621845350216	40.0	37.0	41.0	30.0	41.0
24-25	36.779696929067256	40.0	37.0	41.0	30.0	41.0
26-27	36.65561503680061	40.0	36.5	41.0	30.0	41.0
28-29	36.45710401187735	40.0	36.0	41.0	29.5	41.0
30-31	36.39991508389144	40.0	36.0	41.0	29.5	41.0
32-33	36.2656656049428	40.0	35.5	41.0	29.0	41.0
34-35	36.12294549890122	40.0	35.0	41.0	28.5	41.0
36-37	35.976757349222126	40.0	35.0	41.0	28.0	41.0
38-39	35.798866549811635	40.0	35.0	41.0	27.0	41.0
40-41	35.587773715466724	39.5	35.0	41.0	26.5	41.0
42-43	35.396887154231194	39.0	35.0	41.0	26.0	41.0
44-45	35.16682881392144	39.0	35.0	41.0	25.0	41.0
46-47	34.86329187813939	39.0	34.5	41.0	23.5	41.0
48-49	34.586815547823356	39.0	34.0	41.0	23.0	41.0
50-51	34.35055882211874	39.0	34.0	41.0	22.0	41.0
52-53	34.14758615878331	38.0	34.0	41.0	21.5	41.0
54-55	33.92221673555881	38.0	33.5	40.0	20.5	41.0
56-57	33.660061686811076	37.5	33.0	40.0	20.0	41.0
58-59	33.50887040864379	37.0	33.0	40.0	20.0	41.0
60-61	33.39099698487861	37.0	33.0	40.0	20.0	41.0
62-63	33.12342894298521	36.0	33.0	40.0	18.5	41.0
64-65	32.82762193473559	36.0	33.0	39.0	18.0	41.0
66-67	32.52887610968676	35.0	33.0	39.0	17.5	41.0
68-69	32.21569400115111	35.0	32.0	38.5	15.5	41.0
70-71	31.921124217332917	35.0	32.0	37.5	14.0	40.0
72-73	31.61356166937003	35.0	32.0	37.0	13.0	39.0
74-75	31.327689246285058	35.0	32.0	36.5	11.0	39.0
76-77	31.04673111134366	35.0	32.0	36.0	9.0	39.0
78-79	30.77879789748151	35.0	31.0	35.5	7.5	37.0
80-81	30.558429967646852	35.0	31.0	35.0	7.0	37.0
82-83	30.37569764197014	35.0	31.0	35.0	7.0	36.5
84-85	30.19143431918864	35.0	31.0	35.0	5.5	36.0
86-87	30.049778662184316	35.0	31.0	35.0	2.0	36.0
88-89	29.94557084554207	35.0	31.0	35.0	2.0	36.0
90-91	29.84916735340798	35.0	31.0	35.0	2.0	35.0
92-93	29.91276777460932	35.0	31.0	35.0	2.0	35.0
94-95	29.88320574115739	35.0	31.0	35.0	2.0	35.0
96-97	29.864747933235662	35.0	31.0	35.0	2.0	35.0
98-99	29.82016452359774	35.0	31.0	35.0	2.0	35.0
100-101	29.76885922457095	35.0	31.0	35.0	2.0	35.0
102-103	28.886292534358866	33.5	29.5	35.0	2.0	35.0
104-105	29.594216003034745	34.5	31.0	35.0	2.0	35.0
106-107	29.702133585356496	35.0	31.0	35.0	2.0	35.0
108-109	29.711744693560767	35.0	31.0	35.0	2.0	35.0
110-111	29.675075160021628	35.0	31.0	35.0	2.0	35.0
112-113	29.624410547038508	35.0	31.0	35.0	2.0	35.0
114-115	29.56426645126971	35.0	31.0	35.0	2.0	35.0
116-117	29.487582790480673	35.0	31.0	35.0	2.0	35.0
118-119	29.392797773214035	35.0	31.0	35.0	2.0	35.0
120-121	29.268627040602766	35.0	31.0	35.0	2.0	35.0
122-123	29.147183379552114	35.0	31.0	35.0	2.0	35.0
124-125	28.223596976594113	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29852.0
3	11143.0
4	6389.0
5	5278.0
6	5609.0
7	6942.0
8	7773.0
9	8713.0
10	10159.0
11	11257.0
12	11908.0
13	13662.0
14	17730.0
15	14755.0
16	7764.0
17	6379.0
18	5896.0
19	5318.0
20	5069.0
21	5337.0
22	6471.0
23	8518.0
24	12103.0
25	16548.0
26	20917.0
27	23191.0
28	27158.0
29	33067.0
30	38056.0
31	45684.0
32	56898.0
33	73484.0
34	102577.0
35	164175.0
36	326617.0
37	517117.0
38	164860.0
39	378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49703715789801	17.967281317478395	12.641805240948175	24.89387628367542
2	16.93186550129611	20.07059372417868	37.2144257119872	25.783115062538016
3	18.598315012524292	24.654076050664102	29.66826298015465	27.07934595665695
4	13.268549647043425	15.861751177203558	35.66663667421931	35.20306250153371
5	14.225607030321532	37.29423116761342	33.43420521822563	15.045956583839418
6	32.999077092990106	35.38140917488406	16.90762371342238	14.71189001870345
7	29.09171387329871	31.892168278015447	20.812401062312755	18.203716786373093
8	27.923567976035006	32.99487211910986	19.54405028462791	19.537509620227222
9	27.892617727678793	13.840677306493399	19.121928141108064	39.14477682471975
10-11	26.317335755631337	24.688007853391014	27.00407141200515	21.990584978972503
12-13	26.737127886787487	22.634545056654495	27.25899185876942	23.369335197788597
14-15	24.254421094256088	23.568313812868983	24.961349235310326	27.21591585756461
16-17	23.884432171437386	26.39901469508476	25.489259271574262	24.227293861903597
18-19	23.96411638491955	25.12101058864794	27.66252592110717	23.252347105325338
20-21	25.051178279205143	24.10406327327852	27.481570777291154	23.363187670225187
22-23	24.821553842908514	23.771917058171677	27.2819986187627	24.124530480157112
24-25	24.277435693929924	24.367459831191795	27.281647177335834	24.073457297542447
26-27	23.96262950693679	24.334453921217268	27.48589953409526	24.217017037750686
28-29	24.063411180030776	24.412661085863583	27.468965708968586	24.05496202513705
30-31	24.36049516241929	24.297558640810994	27.260345139632403	24.081601057137313
32-33	24.051061398887402	24.460783418591017	27.106819624466453	24.381335558055127
34-35	24.315722192163914	24.509355251519914	27.25824146620348	23.916681090112693
36-37	24.50317317768841	24.285132194160045	26.93653780898792	24.27515681916362
38-39	24.46117955157557	24.551454276206314	26.80796805277801	24.179398119440112
40-41	24.496690115260684	24.45349222887739	26.891297492805577	24.158520163056348
42-43	24.449361658521376	24.587021243891034	26.70347945435458	24.260137643233012
44-45	24.458151931421014	24.526292635979548	26.504689852131946	24.510865580467495
46-47	24.59065989925194	24.787500742718496	26.146961777116857	24.474877580912707
48-49	24.902582676615918	24.93749918228324	25.59537957321727	24.564538567883577
50-51	24.717418305401097	25.19742083474269	25.471314731588613	24.6138461282676
52-53	24.886882391486143	25.066262387059563	25.58627629916255	24.460578922291745
54-55	24.703206428119753	25.0895096051111	25.653932535269618	24.553351431499532
56-57	24.686373346075104	25.090459398823526	25.462436623906687	24.76073063119468
58-59	24.602927352052227	24.899527462221098	25.748770821221434	24.748774364505234
60-61	24.68542277842997	25.083616962761525	25.806657357570415	24.424302901238086
62-63	24.652347047556432	25.082856934918972	25.722488758196764	24.542307259327828
64-65	24.674608935174724	24.77226782029348	25.85076752033924	24.702355724192557
66-67	24.58390340955548	24.982202720013085	25.74595949960481	24.687934370826632
68-69	24.738322362861837	24.99552967369889	25.51495978341814	24.75118818002113
70-71	24.54848860234045	24.772176273625597	25.892195461650523	24.78713966238343
72-73	24.95137491250755	25.02872805057009	25.507456210165042	24.512440826757317
74-75	24.644610605520022	25.292222685883882	25.401411827042875	24.66175488155322
76-77	24.669066222732443	24.975034150883126	25.443525395937655	24.91237423044678
78-79	24.69055403006915	25.0468390810081	25.408548824000366	24.854058064922388
80-81	24.597570376120693	25.17984493757489	25.320548115471464	24.902036570832955
82-83	24.72220533979511	25.142246182852944	25.310404241616695	24.825144235735248
84-85	24.52400588925005	25.116746587271173	25.22036991511173	25.13887760836705
86-87	24.390826890287247	25.122074464605763	25.364669867476024	25.12242877763097
88-89	24.521070499392845	25.009819407401146	25.336333442816432	25.13277665038957
90-91	24.48560758728468	24.93653732837006	25.429194082668	25.148661001677258
92-93	24.520372664205677	25.145949245827833	25.344163869777383	24.989514220189115
94-95	24.352472252314865	24.83712500596175	25.519803228202143	25.29059951352125
96-97	24.506855863088767	24.87012912688511	25.415638573352584	25.207376436673538
98-99	24.448609765163845	24.803267626882704	25.309364995100086	25.43875761285336
100-101	24.31485447019728	24.99305690124938	25.386694488119876	25.305394140433474
102-103	24.470649729492855	24.733689721818582	25.513189607903623	25.282470940784936
104-105	24.263413405802435	24.9147959381113	25.393808186893533	25.427982469192727
106-107	24.352002467913177	24.95328378794151	25.305947926922954	25.388765817222357
108-109	24.399858811151738	24.881236465427133	25.248790831992086	25.470113891429047
110-111	24.40782594406248	24.887593279387556	25.3080607725276	25.39652000402236
112-113	24.28999745719544	24.889259907603257	25.345629278718363	25.475113356482936
114-115	24.050844030306713	25.029718609327144	25.428201420497746	25.491235939868396
116-117	24.138384947521875	25.09444046087981	25.25474600619256	25.51242858540576
118-119	24.098466053429625	24.698057840439358	25.395001530321764	25.808474575809253
120-121	23.786862594941365	24.9369510297408	25.4650798365932	25.811106538724633
122-123	23.885344937533315	24.91680010987945	25.405343978268284	25.79251097431895
124-125	23.90267889536147	24.998698427400463	25.27825714113599	25.82036553610208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	50.5
2	98.0
3	143.0
4	107.5
5	105.0
6	188.0
7	393.5
8	603.5
9	683.0
10	589.0
11	481.5
12	511.0
13	612.5
14	748.5
15	878.0
16	1038.0
17	1212.0
18	1433.0
19	1652.0
20	1906.0
21	2248.5
22	2552.5
23	2939.0
24	3437.5
25	4146.0
26	5547.5
27	7088.5
28	7941.0
29	9350.0
30	11670.0
31	13707.5
32	16273.0
33	19289.5
34	22275.5
35	25016.5
36	28300.5
37	31572.0
38	33208.5
39	35428.0
40	37827.0
41	39655.5
42	42278.0
43	47571.5
44	52621.5
45	55708.5
46	59789.0
47	63739.5
48	68898.5
49	74304.0
50	74693.5
51	73891.0
52	74008.0
53	74655.5
54	75667.0
55	75414.5
56	72264.5
57	66453.0
58	62877.5
59	59115.5
60	53121.5
61	47160.0
62	40999.5
63	32571.0
64	24537.5
65	19235.5
66	14579.5
67	10862.5
68	8719.5
69	7103.0
70	6155.0
71	5273.5
72	4324.5
73	4079.0
74	3286.0
75	2342.0
76	1561.5
77	860.5
78	495.5
79	298.0
80	167.0
81	58.5
82	31.0
83	16.5
84	10.0
85	6.5
86	4.5
87	1.0
88	1.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	1.0
97	1.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12105178247523371
2	0.0015260918096832706
3	0.017277539416771313
4	0.052159637923817496
5	0.02245535091391098
6	0.01804058532161295
7	0.014388865634156551
8	0.004142249197711734
9	0.003978739360959956
10-11	0.007112677898702386
12-13	0.004278507395004884
14-15	0.002915925422073392
16-17	0.010519132831031116
18-19	0.005368572973350077
20-21	0.012726515627180131
22-23	0.008993041021347846
24-25	0.013298800055811357
26-27	0.00814824019813032
28-29	0.013571316450397655
30-31	0.01983919352588252
32-33	0.011745456606669457
34-35	0.01932141237616855
36-37	0.012781018906097391
38-39	0.019157902539416773
40-41	0.009347312334310031
42-43	0.008393504953257988
44-45	0.017032274661643643
46-47	0.014988401702246407
48-49	0.020493232872889632
50-51	0.015397176294125854
52-53	0.020602239430724152
54-55	0.017005023022185015
56-57	0.019920948444258405
58-59	0.016105718920050232
60-61	0.018830882865913213
62-63	0.02264611239012139
64-65	0.016650751709222825
66-67	0.011009662341286452
68-69	0.023654423050090693
70-71	0.014961150062787776
72-73	0.016787009906515975
74-75	0.017468300892981722
76-77	0.013407806613645877
78-79	0.029786041928282405
80-81	0.021474291893400308
82-83	0.009183802497558253
84-85	0.012154231198548904
86-87	0.011772708246128086
88-89	0.020411477954513743
90-91	0.01139118529370727
92-93	0.0067856582251988274
94-95	0.007603207408957723
96-97	0.002452647551276685
98-99	0.0014170852518487513
100-101	0.010709894307241525
102-103	0.002725163945862983
104-105	0.002125627877773127
106-107	1.9076147621040882E-4
108-109	0.01795883040323706
110-111	4.632778707967071E-4
112-113	0.008911286102971955
114-115	0.0023436409934421655
116-117	0.007112677898702386
118-119	0.009292809055392773
120-121	0.0036517196874563977
122-123	7.630459048416353E-4
124-125	0.023572668131714804
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1834752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.260723079626146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61614477718099	41.871527165652346
2	11.035559596698961	11.755237674169884
3	3.6038161324002824	5.7582555917298235
4	1.808802945313627	3.85352611103845
5	1.0649597286129198	2.836026259830327
6	0.7469863158062617	2.3871018786256486
7	0.4930007834024683	1.838030474198633
8	0.37510945269257495	1.5982880547527487
9	0.3063577398677367	1.46851512717561
>10	1.7914537617267936	17.43065710733317
>50	0.1132070694883065	4.094389051373827
>100	0.04244389992110092	3.99213268139524
>500	0.0017467879570146592	0.5800470442227116
>1k	4.110089310622728E-4	0.5362657785015897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3340	0.18204095158364728	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3142	0.17124930235802985	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	5.450327891725966E-5
9	0.0	0.0	0.0	0.0	5.450327891725966E-5
10-11	0.0	0.0	0.0	5.450327891725966E-5	5.450327891725966E-5
12-13	0.0	0.0	0.0	1.0900655783451932E-4	5.450327891725966E-5
14-15	0.0	0.0	0.0	1.0900655783451932E-4	8.175491837588949E-5
16-17	0.0	0.0	0.0	1.63509836751779E-4	1.0900655783451932E-4
18-19	0.0	0.0	0.0	1.9076147621040882E-4	1.0900655783451932E-4
20-21	0.0	0.0	0.0	2.452647551276685E-4	1.0900655783451932E-4
22-23	0.0	0.0	0.0	2.997680340449282E-4	1.63509836751779E-4
24-25	0.0	0.0	0.0	3.8152295242081764E-4	1.63509836751779E-4
26-27	0.0	0.0	0.0	4.3602623133807733E-4	2.1801311566903864E-4
28-29	0.0	0.0	0.0	0.0012263237756383423	2.1801311566903864E-4
30-31	0.0	0.0	0.0	0.00476903690526022	2.1801311566903864E-4
32-33	0.0	0.0	0.0	0.011391185293707268	2.1801311566903864E-4
34-35	0.0	0.0	0.0	0.02054773615180689	2.725163945862983E-4
36-37	0.0	0.0	0.0	0.03537262801730152	2.725163945862983E-4
38-39	0.0	0.0	0.0	0.05815499860471606	2.725163945862983E-4
40-41	0.0	0.0	0.0	0.08428932084554207	2.725163945862983E-4
42-43	0.0	0.0	0.0	0.10974235209990232	2.725163945862983E-4
44-45	0.0	0.0	0.0	0.14029143993302637	2.725163945862983E-4
46-47	0.0	0.0	0.0	0.17560956467141065	2.997680340449282E-4
48-49	0.0	0.0	0.0	0.2132168271243198	4.90529510255337E-4
50-51	0.0	0.0	0.0	0.25586564287707547	4.90529510255337E-4
52-53	0.0	0.0	0.0	0.29802392911957587	5.450327891725966E-4
54-55	0.0	0.0	0.0	0.344542477675457	7.085426259243756E-4
56-57	0.0	0.0	0.0	0.39648410248360544	7.085426259243756E-4
58-59	0.0	0.0	0.0	0.4554566502720804	7.085426259243756E-4
60-61	0.0	0.0	0.0	0.5188712152923121	7.085426259243756E-4
62-63	0.0	0.0	0.0	0.5820132639179574	7.085426259243756E-4
64-65	0.0	0.0	0.0	0.6496245814148179	7.085426259243756E-4
66-67	0.0	0.0	0.0	0.7274007604297474	7.085426259243756E-4
68-69	0.0	0.0	0.0	0.8124803788195898	7.085426259243756E-4
70-71	0.0	0.0	0.0	0.91192161120413	7.085426259243756E-4
72-73	0.0	0.0	0.0	1.0141697624529091	8.720524626761546E-4
74-75	0.0	0.0	0.0	1.116881191572485	8.720524626761546E-4
76-77	0.0	0.0	0.0	1.2289399330263708	8.993041021347844E-4
78-79	0.0	0.0	0.0	1.357322406515976	9.265557415934143E-4
80-81	0.0	0.0	0.0	1.5066886423887262	9.265557415934143E-4
82-83	0.0	0.0	0.0	1.6613689479559088	9.81059020510674E-4
84-85	0.0	0.0	0.0	1.8211453101018558	9.81059020510674E-4
86-87	0.0	0.0	0.0	1.994193220664155	9.81059020510674E-4
88-89	0.0	0.0	0.0	2.1893967141063206	0.0010355622994279337
90-91	0.0	0.0	0.0	2.40441214943491	0.0010355622994279337
92-93	0.0	0.0	0.0	2.6405476053439374	0.0010355622994279337
94-95	0.0	0.0	0.0	2.8840410039067947	0.0010900655783451932
96-97	0.0	0.0	0.0	3.1377537672666387	0.0011718204967210827
98-99	0.0	0.0	0.0	3.4199717455002094	0.0012535754150969722
100-101	0.0	0.0	0.0	3.7447840362076183	0.0012535754150969722
102-103	0.0	0.0	0.0	4.087773170433934	0.0012535754150969722
104-105	0.0	0.0	0.0	4.432479157946142	0.0012535754150969722
106-107	0.0	0.0	0.0	4.801875130807869	0.0012808270545556022
108-109	0.0	0.0	0.0	5.195661320985071	0.0014170852518487513
110-111	0.0	0.0	0.0	5.62250374982559	0.001444336891307381
112-113	0.0	0.0	0.0	6.078069406655505	0.0014715885307660108
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2875	0.0	80.88295	1
GTATCAA	5705	0.0	58.005287	1
TCAACGC	7670	0.0	42.780037	4
CAACGCA	7995	0.0	40.93414	5
ATCAACG	8245	0.0	40.013264	3
AACGCAG	8230	0.0	39.7588	6
GTACATG	9230	0.0	38.049053	1
TATCAAC	8640	0.0	38.04611	2
TACATGG	9735	0.0	35.295937	2
ACGCAGA	9550	0.0	34.201046	7
ACATGGG	10035	0.0	33.82536	3
CGCAGAG	10015	0.0	32.730103	8
CATGGGG	7870	0.0	31.250734	4
GTGGTAT	1055	0.0	29.953918	1
GCAGAGT	10730	0.0	29.828354	9
AGAGTAC	10035	0.0	27.477617	10-11
CAGAGTA	10385	0.0	26.408344	10-11
ATGGGGG	4035	0.0	26.24931	5
GAGTACT	6045	0.0	24.997467	12-13
AGTACTT	6450	0.0	24.719156	12-13
>>END_MODULE