FastQCFastQC Report
Mon 15 Aug 2016
SRR2921529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792082
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.077.22461
GTATCAA40300.060.407111
TCAACGC51900.045.858184
CAACGCA53800.044.237425
AACGCAG56100.042.4202086
ATCAACG56300.042.274243
TATCAAC58200.041.2020072
ACGCAGA67250.035.3857
GTACATG90100.035.342621
TACATGG93450.033.874172
CGCAGAG70400.033.629898
ACATGGG100050.031.5792373
GCAGAGT77350.030.1476149
CATGGGG84550.028.4309884
TACCGTA2757.4578566E-1125.9597197
CAGAGTA74050.025.82754310-11
ACCGTAT2809.458745E-1125.4940178
AGAGTAC71250.025.08919710-11
GTATAGG5000.024.9987951
CCGTATG3002.237357E-1023.7950829
Produced by FastQC (version 0.11.5)