##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921529_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1792082
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.86726388636234	31.0	30.0	34.0	25.0	34.0
2	29.90927982090105	31.0	30.0	34.0	25.0	34.0
3	29.978007144762348	31.0	30.0	34.0	25.0	34.0
4	33.35150512085942	37.0	35.0	37.0	27.0	37.0
5	33.337592811043244	37.0	35.0	37.0	27.0	37.0
6	33.35471870148799	37.0	35.0	37.0	27.0	37.0
7	33.15257616559956	37.0	35.0	37.0	26.0	37.0
8	33.119433708948584	37.0	35.0	37.0	26.0	37.0
9	34.56336763607915	39.0	35.0	39.0	26.0	39.0
10-11	34.47185703555976	39.0	35.0	39.0	25.5	39.0
12-13	34.359787107955995	39.0	35.0	39.0	25.0	39.0
14-15	35.5287344552314	40.0	36.0	41.0	25.0	41.0
16-17	35.482679922012494	40.0	36.0	41.0	25.0	41.0
18-19	35.51002325786432	40.0	36.0	41.0	25.0	41.0
20-21	35.5291800263604	40.0	36.0	41.0	25.0	41.0
22-23	35.50279618901367	40.0	36.0	41.0	25.0	41.0
24-25	35.478903867122156	40.0	35.5	41.0	25.0	41.0
26-27	35.41376147966444	40.0	35.0	41.0	25.0	41.0
28-29	35.36771726963387	40.0	35.0	41.0	25.0	41.0
30-31	35.27759416142788	40.0	35.0	41.0	25.0	41.0
32-33	35.16364820359782	40.0	35.0	41.0	24.5	41.0
34-35	35.00468868054028	40.0	35.0	41.0	24.0	41.0
36-37	34.86308439011161	39.0	35.0	41.0	23.5	41.0
38-39	34.73058459378533	39.0	34.0	41.0	23.0	41.0
40-41	34.578154068842835	39.0	34.0	41.0	22.0	41.0
42-43	34.387932862447144	39.0	34.0	41.0	20.5	41.0
44-45	34.18820539461922	39.0	33.0	41.0	19.0	41.0
46-47	33.917313493467375	38.5	33.0	41.0	17.0	41.0
48-49	33.757279242802504	38.0	33.0	40.0	15.0	41.0
50-51	33.56944492495322	38.0	33.0	40.0	14.0	41.0
52-53	33.36266923053744	38.0	33.0	40.0	12.5	41.0
54-55	33.154348963942496	38.0	33.0	40.0	10.0	41.0
56-57	32.9092954451861	37.0	32.0	40.0	8.0	41.0
58-59	32.65750311648686	37.0	32.0	40.0	7.5	41.0
60-61	32.34602211282743	36.0	32.0	40.0	7.0	41.0
62-63	32.03450483850627	36.0	31.0	39.0	4.0	41.0
64-65	31.71831143887389	35.0	31.0	39.0	2.0	41.0
66-67	31.673519403688	35.0	31.0	39.0	2.0	41.0
68-69	31.42252642457209	35.0	31.0	38.5	2.0	40.0
70-71	31.123053520988435	35.0	31.0	37.0	2.0	40.0
72-73	30.79466313483423	35.0	31.0	37.0	2.0	39.0
74-75	30.497924760139327	35.0	31.0	36.0	2.0	39.0
76-77	30.222682890626658	35.0	31.0	36.0	2.0	38.5
78-79	29.958530636432933	35.0	30.0	35.5	2.0	37.0
80-81	29.72027368167305	35.0	30.0	35.0	2.0	37.0
82-83	29.523799134191403	35.0	30.0	35.0	2.0	36.5
84-85	29.325178200551093	35.0	30.0	35.0	2.0	36.0
86-87	29.176001432970143	34.0	30.0	35.0	2.0	36.0
88-89	29.054630033670335	34.0	30.0	35.0	2.0	35.5
90-91	28.948853623885512	34.0	30.0	35.0	2.0	35.0
92-93	28.845444293285688	34.0	29.5	35.0	2.0	35.0
94-95	28.777819876545827	34.0	29.0	35.0	2.0	35.0
96-97	28.679442402747195	34.0	29.0	35.0	2.0	35.0
98-99	28.61579101849134	34.0	29.0	35.0	2.0	35.0
100-101	28.546685363727775	34.0	29.0	35.0	2.0	35.0
102-103	27.57821907702884	32.5	27.5	34.5	2.0	35.0
104-105	28.397580858465183	34.0	29.0	35.0	2.0	35.0
106-107	28.724618348937156	34.0	30.0	35.0	2.0	35.0
108-109	28.764134397867956	35.0	30.0	35.0	2.0	35.0
110-111	28.7049387249021	35.0	30.0	35.0	2.0	35.0
112-113	28.631229207145655	35.0	30.0	35.0	2.0	35.0
114-115	28.512711471908094	35.0	30.0	35.0	2.0	35.0
116-117	28.445203679295926	34.5	30.0	35.0	2.0	35.0
118-119	28.288059084349936	34.0	29.0	35.0	2.0	35.0
120-121	28.11428857608078	34.0	29.0	35.0	2.0	35.0
122-123	27.990744564143828	34.0	29.0	35.0	2.0	35.0
124-125	27.13236336283719	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69220.0
3	11664.0
4	7730.0
5	7009.0
6	7406.0
7	8459.0
8	9335.0
9	9741.0
10	10442.0
11	10683.0
12	10861.0
13	11650.0
14	12847.0
15	11209.0
16	8043.0
17	7023.0
18	6548.0
19	6082.0
20	5888.0
21	6015.0
22	7103.0
23	9746.0
24	14018.0
25	19480.0
26	22904.0
27	24019.0
28	28415.0
29	33260.0
30	38215.0
31	45811.0
32	58233.0
33	76200.0
34	107255.0
35	169703.0
36	325757.0
37	457071.0
38	116891.0
39	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53064361813174	17.14110119868309	12.348601648035743	24.979653535149428
2	16.74304949730218	19.399118011333794	37.98043460909684	25.877397882267182
3	19.287402706496653	23.88593531534398	28.776104241145557	28.050557737013808
4	13.647892544084877	15.23334062685594	35.34219645177568	35.776570377283505
5	14.648265791575705	36.8306271417001	33.193916413481595	15.327190653242601
6	33.69650109558834	34.30896986783157	17.184016971151834	14.810512065428256
7	29.849813103728547	31.26828211735389	20.368567485854385	18.513337293063177
8	27.992055570813267	33.00644619647614	19.5585795592829	19.44291867342769
9	27.857500487006554	14.694369199528678	18.764516004793595	38.683614308671174
10-11	26.594970014287416	24.72032918833573	26.604654979397818	22.080045817979034
12-13	26.81691277707831	22.436773576095412	26.682467748683532	24.06384589814275
14-15	24.215817479245366	23.519740101341498	24.247579149496154	28.016863269916982
16-17	24.409795837254816	26.5354072258004	24.628812770247414	24.42598416669737
18-19	24.47767426838667	25.676901265104785	26.20074134844577	23.64468311806277
20-21	25.640039229404078	24.597523120418245	25.867788644745808	23.89464900543187
22-23	25.4956325599703	24.273475164633926	25.624603943397755	24.606288331998023
24-25	24.801603199698928	24.786499218138623	25.767113355521204	24.644784226641246
26-27	24.50410430606704	24.772300128096443	25.953416886790265	24.770178679046253
28-29	24.612976262023007	24.775018332666647	25.973403885604164	24.638601519706185
30-31	24.860353207082007	24.791461440569268	25.643787696120317	24.704397656228412
32-33	24.51436057790149	24.837938736501407	25.75376179499756	24.89393889059954
34-35	24.8992121184379	24.82198141550732	25.781601508552594	24.49720495750218
36-37	25.029122522282606	24.643585829021134	25.60090936523928	24.72638228345698
38-39	24.89519715999379	24.867949538297985	25.599252253791494	24.63760104791673
40-41	24.916119185007858	24.787213493282838	25.696420117550378	24.600247204158926
42-43	24.86773003750906	24.800059630358774	25.58912896125925	24.743081370872915
44-45	24.805337433725953	24.818039494195578	25.555819832857633	24.820803239220837
46-47	24.972653796222623	24.866926309030248	25.399109398774378	24.76131049597275
48-49	25.022394186248725	25.001849317343694	25.147756966483115	24.827999529924465
50-51	24.96498104873175	25.261295251770765	25.06360357151431	24.71012012798317
52-53	25.016722576091908	25.029397116235025	25.222781564630104	24.731098743042963
54-55	24.947117786790752	25.045429296383244	25.23655334697375	24.770899569852254
56-57	24.838529142073977	25.172901311501494	25.189007225330666	24.799562321093862
58-59	24.949231968660737	24.96497529219357	25.13806810997214	24.94772462917355
60-61	24.915418292200286	25.03961845709345	25.312440167617194	24.732523083089074
62-63	24.817585482425375	25.152171431266968	25.250813855827424	24.77942923048023
64-65	24.88556709289476	24.959562247900795	25.24144722353487	24.91342343566957
66-67	24.796594459130077	25.10347160528147	25.256030994173102	24.84390294141535
68-69	24.852965433166222	25.022683499111498	25.21506413199438	24.909286935727902
70-71	24.790431096424307	24.898348962721162	25.316453929455324	24.994766011399207
72-73	24.997076481205333	25.076451063086324	25.132074671275078	24.79439778443326
74-75	24.726316515183953	25.26551924430115	25.19861433997907	24.809549900535828
76-77	24.80861521114496	24.982549369028163	25.165163488898163	25.043671930928713
78-79	24.771249819980643	25.028663547447522	25.225010242961943	24.975076389609896
80-81	24.77054337293986	25.25264787700991	25.058633954445796	24.918174795604433
82-83	24.949323683252928	25.151052259114483	25.010994751540938	24.88862930609165
84-85	24.79539259394862	25.112517329678006	25.043645114984226	25.048444961389144
86-87	24.576862886050176	25.1816115767055	25.1881415565686	25.053383980675726
88-89	24.71627687615029	25.085095309257994	25.129299939860495	25.069327874731222
90-91	24.66343350010743	25.075662236409958	25.171206214823265	25.089698048659343
92-93	24.709348322395435	25.240099313140853	25.093228159857656	24.957324204606053
94-95	24.70988094424206	25.000258119245512	25.23465829895232	25.05520263756012
96-97	24.731597456597267	24.991552034306412	25.1912616642618	25.08558884483452
98-99	24.663447143936217	25.089898573035423	25.042015915681876	25.204638367346483
100-101	24.585786210239256	25.185163895475004	25.133178763836543	25.095871130449193
102-103	24.661934040864796	24.94835258415655	25.24356039594678	25.14615297903188
104-105	24.445417310865142	25.23528067521766	25.1772435197191	25.1420584941981
106-107	24.465991189328975	25.194400636636278	25.107650015235073	25.231958158799678
108-109	24.420470343071734	25.17271342714002	25.215741798518955	25.191074431269296
110-111	24.456847922440687	25.163583887123142	25.23434398055102	25.145224209885153
112-113	24.53223828056551	25.18912484695341	25.210833187308936	25.06780368517214
114-115	24.273321069133868	25.450287998486555	25.181695891567585	25.09469504081199
116-117	24.254068047543658	25.44385929764899	25.192541322233343	25.109531332574015
118-119	24.449457294923313	25.19946449631247	25.116795345469516	25.2342828632947
120-121	24.194211324131466	25.242734756526076	25.330454693338606	25.23259922600385
122-123	24.184523777961285	25.330087828995936	25.21616772433009	25.269220668712688
124-125	24.21450005650025	25.42181969863744	25.165071406549284	25.19860883831303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	159.0
2	193.0
3	192.0
4	173.0
5	157.5
6	154.0
7	192.5
8	277.0
9	323.0
10	361.5
11	408.5
12	461.5
13	517.5
14	597.0
15	714.5
16	789.0
17	840.5
18	940.5
19	1091.0
20	1194.5
21	1344.0
22	1602.5
23	1955.5
24	2383.5
25	3014.0
26	3992.0
27	5128.5
28	6076.5
29	7402.5
30	9397.0
31	11319.0
32	13373.5
33	15495.0
34	18024.0
35	20845.0
36	23916.5
37	27306.0
38	30115.5
39	33009.5
40	36168.5
41	39347.0
42	42732.0
43	47615.5
44	52756.0
45	56819.0
46	61282.0
47	65555.0
48	70146.0
49	74266.0
50	74964.5
51	75404.0
52	75590.0
53	75496.5
54	76577.0
55	75958.5
56	71957.0
57	65851.5
58	62093.5
59	58435.5
60	52179.5
61	45895.0
62	39637.5
63	31458.5
64	24097.5
65	19172.5
66	14918.5
67	11680.5
68	9526.5
69	7819.0
70	6657.5
71	5537.0
72	4444.0
73	3955.5
74	3306.0
75	2541.0
76	1774.0
77	1106.5
78	672.5
79	421.0
80	266.5
81	125.0
82	71.0
83	43.5
84	31.0
85	24.5
86	16.0
87	12.5
88	11.5
89	12.5
90	12.0
91	11.5
92	10.5
93	11.0
94	10.0
95	8.0
96	6.5
97	6.5
98	8.5
99	12.5
100	31.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03437342710880417
2	0.025054657097164083
3	0.026059075421771997
4	0.03002094770216988
5	0.031973983333351934
6	0.04503142155325482
7	0.039841926876113924
8	0.03560104950554718
9	0.02929553446773083
10-11	0.036019557140800476
12-13	0.03590795510473293
14-15	0.034289725581753513
16-17	0.03727508004656037
18-19	0.04946760248693978
20-21	0.06018139794942419
22-23	0.05507560479933395
24-25	0.0649244844822949
26-27	0.04762616889182526
28-29	0.04913279637873714
30-31	0.048351582126264316
32-33	0.056665933813296494
34-35	0.038837508551506014
36-37	0.052592459496831064
38-39	0.061381119837150305
40-41	0.04743086532870706
42-43	0.05847946689939411
44-45	0.05769825264692129
46-47	0.06456177786507537
48-49	0.04913279637873714
50-51	0.050723125392699665
52-53	0.06082310965681258
54-55	0.046705452094268005
56-57	0.04544992918850811
58-59	0.04723556176558885
60-61	0.03454083016290549
62-63	0.04260407726878569
64-65	0.04176706199827909
66-67	0.03618696019490179
68-69	0.032448291986639004
70-71	0.05049992132056458
72-73	0.03216928689647014
74-75	0.0409300467277725
76-77	0.03345271031124692
78-79	0.03392701896453399
80-81	0.025556866259468035
82-83	0.017744723734739818
84-85	0.019976764456090736
86-87	0.01930715223968546
88-89	0.02312952197499891
90-91	0.013224841274004202
92-93	0.020953282271681764
94-95	0.015317379450270691
96-97	0.012862134656784678
98-99	0.01124390513380526
100-101	0.012471527530548266
102-103	0.006919326236187853
104-105	0.007058828781272285
106-107	0.009151366957538773
108-109	0.013448045346139295
110-111	0.006221813510765691
112-113	0.008035346596863313
114-115	0.008119048123913973
116-117	0.007198331326356718
118-119	0.23617780882794426
120-121	0.35070939834226333
122-123	0.026533384075059063
124-125	0.003041155482840629
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1792082.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.00392155790421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.13897449791523	48.68656661061696
2	9.947701746825572	11.938022305958967
3	3.273952926803709	5.8935004381260185
4	1.6056860569183662	3.8538984082380066
5	0.9507128660171513	2.852325011829172
6	0.6140504277970517	2.2107260221279086
7	0.4174713781186728	1.7534943877712106
8	0.33022584935378224	1.5851876768813313
9	0.23934103316260308	1.2925260521528927
>10	1.3942582411420275	14.955906410331371
>50	0.0662590410752014	2.69857536573304
>100	0.020991194508580273	2.0293406285050253
>500	1.8737018106276799E-4	0.08763876131665509
>1k	1.8737018106276799E-4	0.16229192041127852
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.6740305410131902E-4
14-15	0.0	0.0	0.0	0.0	1.6740305410131902E-4
16-17	0.0	0.0	0.0	2.7900509016886503E-5	1.6740305410131902E-4
18-19	0.0	0.0	0.0	5.5801018033773005E-5	1.6740305410131902E-4
20-21	0.0	0.0	0.0	5.5801018033773005E-5	2.2320407213509202E-4
22-23	0.0	0.0	0.0	5.5801018033773005E-5	2.2320407213509202E-4
24-25	0.0	0.0	0.0	1.1160203606754601E-4	2.2320407213509202E-4
26-27	0.0	0.0	0.0	2.511045811519785E-4	2.2320407213509202E-4
28-29	0.0	0.0	0.0	9.486173065741411E-4	2.2320407213509202E-4
30-31	0.0	0.0	0.0	0.004101374825482316	2.2320407213509202E-4
32-33	0.0	0.0	0.0	0.0101278847731298	2.511045811519785E-4
34-35	0.0	0.0	0.0	0.01844223646016198	3.3480610820263803E-4
36-37	0.0	0.0	0.0	0.03074636093660893	3.3480610820263803E-4
38-39	0.0	0.0	0.0	0.051197434045986735	3.9060712623641104E-4
40-41	0.0	0.0	0.0	0.07301563209719197	3.9060712623641104E-4
42-43	0.0	0.0	0.0	0.09475012862134657	3.9060712623641104E-4
44-45	0.0	0.0	0.0	0.12025119386278083	4.4640814427018404E-4
46-47	0.0	0.0	0.0	0.15085805225430532	5.301096713208435E-4
48-49	0.0	0.0	0.0	0.18302733915077546	6.696122164052761E-4
50-51	0.0	0.0	0.0	0.21678695506120813	6.696122164052761E-4
52-53	0.0	0.0	0.0	0.25004436180933687	6.975127254221626E-4
54-55	0.0	0.0	0.0	0.2889655718878935	7.812142524728221E-4
56-57	0.0	0.0	0.0	0.33438760056738476	8.091147614897086E-4
58-59	0.0	0.0	0.0	0.38156736131493985	8.649157795234815E-4
60-61	0.0	0.0	0.0	0.4373962798577297	8.928162885403681E-4
62-63	0.0	0.0	0.0	0.493141496873469	8.928162885403681E-4
64-65	0.0	0.0	0.0	0.5505328439212045	8.928162885403681E-4
66-67	2.7900509016886503E-5	0.0	0.0	0.6200051113732519	8.928162885403681E-4
68-69	5.5801018033773005E-5	0.0	0.0	0.7019210058468306	8.928162885403681E-4
70-71	5.5801018033773005E-5	0.0	0.0	0.7894449026328036	9.486173065741411E-4
72-73	5.5801018033773005E-5	0.0	0.0	0.8796472482843978	0.0010881198516585738
74-75	5.5801018033773005E-5	0.0	0.0	0.9743973769057442	0.00111602036067546
76-77	5.5801018033773005E-5	0.0	0.0	1.0827071529092978	0.00111602036067546
78-79	5.5801018033773005E-5	0.0	0.0	1.205078785457362	0.0011439208696923467
80-81	5.5801018033773005E-5	0.0	0.0	1.3389733282294003	0.0011997218877261196
82-83	5.5801018033773005E-5	0.0	0.0	1.4846976868245985	0.0012834234147767792
84-85	1.1160203606754601E-4	0.0	0.0	1.6333794993755866	0.0012834234147767792
86-87	1.1160203606754601E-4	0.0	0.0	1.792021793645603	0.0013113239237936658
88-89	1.1160203606754601E-4	0.0	0.0	1.9695806330290688	0.0013950254508443252
90-91	1.1160203606754601E-4	0.0	0.0	2.1728079407080703	0.0013950254508443252
92-93	1.1160203606754601E-4	0.0	0.0	2.3850192122905094	0.0014508264688780981
94-95	1.1160203606754601E-4	0.0	0.0	2.6088649961329895	0.0015066274869118712
96-97	1.1160203606754601E-4	0.0	0.0	2.846744736010964	0.0015345279959287578
98-99	1.1160203606754601E-4	0.0	0.0	3.1057730617237382	0.0015624285049456441
100-101	1.1160203606754601E-4	0.0	0.0	3.392506592890281	0.0015624285049456441
102-103	1.1160203606754601E-4	0.0	0.0	3.7081450513983176	0.0015624285049456441
104-105	1.1160203606754601E-4	0.0	0.0	4.033353384499147	0.0015624285049456441
106-107	1.1160203606754601E-4	0.0	0.0	4.375274122501091	0.0015624285049456441
108-109	1.1160203606754601E-4	0.0	0.0	4.733405056241846	0.0015624285049456441
110-111	1.1160203606754601E-4	0.0	0.0	5.122505554991346	0.0016461300319963036
112-113	1.1160203606754601E-4	0.0	0.0	5.53297784364778	0.0016740305410131902
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1950	0.0	77.2246	1
GTATCAA	4030	0.0	60.40711	1
TCAACGC	5190	0.0	45.85818	4
CAACGCA	5380	0.0	44.23742	5
AACGCAG	5610	0.0	42.420208	6
ATCAACG	5630	0.0	42.27424	3
TATCAAC	5820	0.0	41.202007	2
ACGCAGA	6725	0.0	35.385	7
GTACATG	9010	0.0	35.34262	1
TACATGG	9345	0.0	33.87417	2
CGCAGAG	7040	0.0	33.62989	8
ACATGGG	10005	0.0	31.579237	3
GCAGAGT	7735	0.0	30.147614	9
CATGGGG	8455	0.0	28.430988	4
TACCGTA	275	7.4578566E-11	25.959719	7
CAGAGTA	7405	0.0	25.827543	10-11
ACCGTAT	280	9.458745E-11	25.494017	8
AGAGTAC	7125	0.0	25.089197	10-11
GTATAGG	500	0.0	24.998795	1
CCGTATG	300	2.237357E-10	23.795082	9
>>END_MODULE