FastQCFastQC Report
Mon 15 Aug 2016
SRR2921529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792082
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32570.18174391573599868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.15641025354866572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.076.634651
GTATCAA56350.058.705631
TCAACGC73100.044.715934
CAACGCA75350.043.4378625
ATCAACG77450.042.66573
TATCAAC77650.042.632492
AACGCAG79050.041.618946
ACGCAGA92700.035.296087
GTACATG86400.034.0105671
CGCAGAG99300.032.7685628
TACATGG90150.032.362172
GTGGTAT9050.030.2963241
GCAGAGT105050.030.295429
ACATGGG95500.030.2374843
CATGGGG82200.027.5969094
CAGAGTA100150.026.43189610-11
AGAGTAC97350.026.2144410-11
GAGTACT57050.025.80707712-13
GTACTTT62700.023.3405514-15
AGTACTT62200.022.52266712-13
Produced by FastQC (version 0.11.5)