FastQCFastQC Report
Mon 15 Aug 2016
SRR2921528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815586
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.067.885361
GTATCAA36600.056.920581
TCAACGC45900.044.4627384
CAACGCA47050.043.1230555
ATCAACG50250.040.9666863
AACGCAG49550.040.7037936
TATCAAC53850.038.6699072
GTACATG87050.038.154681
TACATGG92200.035.5558852
ACATGGG95750.034.4240343
ACGCAGA59800.033.7260447
CATGGGG79150.032.625234
CGCAGAG64300.031.2714988
GCAGAGT71100.027.5276559
ATGGGGG44200.027.3267845
TACCGTA2006.12672E-826.7719127
AGAGTAC62450.026.05432310-11
CAGAGTA67650.023.39207610-11
ATGGGGA33000.022.7180675
GAGTACA61150.020.3438051
Produced by FastQC (version 0.11.5)