##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921528_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1815586
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.92412532372468	31.0	30.0	34.0	26.0	34.0
2	30.035623209255853	31.0	30.0	34.0	25.0	34.0
3	30.198156407903564	31.0	31.0	34.0	26.0	34.0
4	33.56836635664739	37.0	35.0	37.0	27.0	37.0
5	33.63710449408621	37.0	35.0	37.0	28.0	37.0
6	33.622753204750424	37.0	35.0	37.0	28.0	37.0
7	33.4546377863676	37.0	35.0	37.0	27.0	37.0
8	33.45172632968089	37.0	35.0	37.0	27.0	37.0
9	34.928717229588685	39.0	35.0	39.0	27.0	39.0
10-11	34.81903198196065	39.0	35.0	39.0	27.0	39.0
12-13	34.68844714599033	39.0	35.0	39.0	26.5	39.0
14-15	35.86957268892799	40.0	36.0	41.0	26.5	41.0
16-17	35.80672025450736	40.0	36.0	41.0	26.0	41.0
18-19	35.821211443577994	40.0	36.0	41.0	26.0	41.0
20-21	35.84069991727189	40.0	36.0	41.0	26.0	41.0
22-23	35.81295956236719	40.0	36.0	41.0	26.0	41.0
24-25	35.7760235538278	40.0	36.0	41.0	26.0	41.0
26-27	35.702362487924006	40.0	36.0	41.0	26.0	41.0
28-29	35.66493159784224	40.0	36.0	41.0	26.0	41.0
30-31	35.568267215103006	40.0	35.0	41.0	25.0	41.0
32-33	35.46583967931015	40.0	35.0	41.0	25.0	41.0
34-35	35.31307935839999	40.0	35.0	41.0	25.0	41.0
36-37	35.18315904617022	40.0	35.0	41.0	24.0	41.0
38-39	35.06850515480952	40.0	35.0	41.0	24.0	41.0
40-41	34.92189849448057	39.5	35.0	41.0	23.5	41.0
42-43	34.73826274271778	39.0	34.5	41.0	23.0	41.0
44-45	34.5578273350863	39.0	34.0	41.0	22.0	41.0
46-47	34.32020956319337	39.0	34.0	41.0	20.0	41.0
48-49	34.174373177585636	39.0	33.5	41.0	19.5	41.0
50-51	33.98841283200025	39.0	33.0	41.0	18.0	41.0
52-53	33.80675467865471	38.0	33.0	40.0	17.5	41.0
54-55	33.608303600049794	38.0	33.0	40.0	15.5	41.0
56-57	33.37614054085017	38.0	33.0	40.0	14.5	41.0
58-59	33.14170961882279	37.0	33.0	40.0	13.5	41.0
60-61	32.8393083555392	37.0	32.0	40.0	11.5	41.0
62-63	32.53063033092346	36.0	32.0	39.5	8.0	41.0
64-65	32.221375632991226	36.0	32.0	39.0	8.0	41.0
66-67	32.17342665122996	35.0	32.0	39.0	7.5	41.0
68-69	31.929384507261016	35.0	32.0	39.0	7.0	41.0
70-71	31.62417505973278	35.0	32.0	37.5	3.5	40.0
72-73	31.288825205746242	35.0	31.5	37.0	2.0	39.5
74-75	30.982057032825765	35.0	31.0	36.5	2.0	39.0
76-77	30.69602761863112	35.0	31.0	36.0	2.0	39.0
78-79	30.41366947090361	35.0	31.0	36.0	2.0	37.5
80-81	30.163577764975056	35.0	31.0	35.0	2.0	37.0
82-83	29.95713505171333	35.0	31.0	35.0	2.0	37.0
84-85	29.74924129179229	35.0	30.5	35.0	2.0	36.0
86-87	29.590296741658065	35.0	30.0	35.0	2.0	36.0
88-89	29.457826563985403	35.0	30.0	35.0	2.0	36.0
90-91	29.34309996882549	35.0	30.0	35.0	2.0	35.0
92-93	29.241020530010694	34.5	30.0	35.0	2.0	35.0
94-95	29.17464664301223	34.0	30.0	35.0	2.0	35.0
96-97	29.07487747757473	34.0	30.0	35.0	2.0	35.0
98-99	29.011872475333035	34.0	30.0	35.0	2.0	35.0
100-101	28.946228380258496	34.0	30.0	35.0	2.0	35.0
102-103	27.981231679468777	33.5	28.0	34.5	2.0	35.0
104-105	28.796356383008018	34.0	29.5	35.0	2.0	35.0
106-107	29.109256185055404	35.0	30.0	35.0	2.0	35.0
108-109	29.151530965759815	35.0	31.0	35.0	2.0	35.0
110-111	29.091232527679768	35.0	31.0	35.0	2.0	35.0
112-113	29.026440499100566	35.0	30.0	35.0	2.0	35.0
114-115	28.9098690450356	35.0	30.0	35.0	2.0	35.0
116-117	28.849114555851386	35.0	30.0	35.0	2.0	35.0
118-119	28.69332270682854	35.0	30.0	35.0	2.0	35.0
120-121	28.526623635564498	35.0	30.0	35.0	2.0	35.0
122-123	28.400149593574746	35.0	30.0	35.0	2.0	35.0
124-125	27.568569321420192	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64573.0
3	11486.0
4	7571.0
5	6416.0
6	6781.0
7	7930.0
8	8624.0
9	8970.0
10	9760.0
11	10173.0
12	10147.0
13	10942.0
14	12124.0
15	10742.0
16	7766.0
17	6935.0
18	6322.0
19	5799.0
20	5578.0
21	5897.0
22	6968.0
23	9401.0
24	13352.0
25	18322.0
26	21922.0
27	23326.0
28	27404.0
29	32982.0
30	37239.0
31	44273.0
32	56370.0
33	73063.0
34	102230.0
35	160793.0
36	314720.0
37	495637.0
38	152877.0
39	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53781493158369	17.409050987427534	12.18010951458964	24.873024566399142
2	16.6058352941695	19.54072635137927	38.40254703940826	25.450891315042966
3	18.82963233118786	24.495038671969475	29.107192341743527	27.56813665509914
4	13.22505928296265	15.34156022179321	36.04861423320022	35.38476626204392
5	14.195088248225614	37.37731364401205	33.734877360476126	14.692720747286216
6	33.173058908840844	35.10996228793854	17.386175035981847	14.330803767238775
7	29.464508080921618	31.262852004416793	21.099767920074584	18.172871994587005
8	27.605161839150377	33.65206400839223	19.6634512609283	19.079322891529092
9	27.791486855537904	14.420756113027444	19.106671316550777	38.68108571488387
10-11	26.175280574485193	24.957747503211465	27.307716359643337	21.559255562660002
12-13	26.388425542418403	22.74726149609655	27.31823449797286	23.54607846351219
14-15	23.912313454935934	23.857299999090912	24.554320760127833	27.676065785845317
16-17	24.069621914798255	26.841589234449298	25.04233530553735	24.046453545215098
18-19	24.084945593370197	26.152488449285	26.560348749073203	23.202217208271605
20-21	25.260534924630868	25.04204931729103	26.167901876843448	23.52951388123465
22-23	25.029117112927008	24.745151211980023	26.014814390287516	24.210917284805454
24-25	24.456173089895813	25.242193803542218	26.11209640795919	24.189536698602776
26-27	24.2208277942706	25.215751372424766	26.201059367272578	24.362361466032056
28-29	24.213332833726543	25.242421796154368	26.243132358236803	24.301113011882283
30-31	24.590568634393033	25.21523025994046	26.043777560728316	24.150423544938192
32-33	24.15322830631884	25.318022442808036	26.006681867340824	24.5220673835323
34-35	24.441603193074325	25.297367168284364	26.145279592358484	24.115750046282827
36-37	24.58109994362683	25.152711781000974	25.9119035561387	24.3542847192335
38-39	24.513757847770883	25.375359322093676	25.925392168101013	24.185490662034425
40-41	24.494408336111487	25.233047987193864	26.079884061484883	24.192659615209767
42-43	24.386143677318454	25.25520860313657	25.91582469687434	24.442823022670638
44-45	24.348272231515768	25.31720141377029	25.90361794365144	24.430908411062504
46-47	24.55941558531027	25.303629444491904	25.778507714395154	24.358447255802677
48-49	24.504410778511083	25.51404897289392	25.58177146386931	24.399768784725687
50-51	24.49551445953095	25.564026406277552	25.473130841121495	24.467328293070008
52-53	24.554517157991963	25.478190711956916	25.62817296649083	24.33911916356029
54-55	24.427407936596712	25.444161614903717	25.729235057062905	24.399195391436663
56-57	24.395432187982397	25.504103829037383	25.556119734663778	24.544344248316442
58-59	24.48015419736535	25.37490005879381	25.612802833563936	24.532142910276907
60-61	24.558017556159463	25.51075531705355	25.676151804444274	24.255075322342712
62-63	24.376873937763293	25.59330284983179	25.691023805397823	24.33879940700709
64-65	24.42452086337436	25.254162949706288	25.74510477007007	24.57621141684928
66-67	24.303628901070716	25.534263526939554	25.65652258114814	24.50558499084159
68-69	24.409471811004536	25.5130105175007	25.63124287217573	24.446274799319035
70-71	24.381626123198398	25.312007591172176	25.780256615343415	24.52610967028601
72-73	24.51676458507745	25.56021754994335	25.55297249662058	24.37004536835862
74-75	24.320840452536107	25.7003174232352	25.557722156318135	24.421119967910553
76-77	24.386748038745214	25.350006638983118	25.677906290615564	24.585339031656105
78-79	24.316073417908424	25.42216840015118	25.731225971524502	24.530532210415892
80-81	24.37326288939512	25.64535810991348	25.445017092204246	24.53636190848715
82-83	24.495620637198044	25.493155171212305	25.519459718373326	24.491764473216325
84-85	24.303908271901054	25.555452112568982	25.515072622485373	24.625566993044593
86-87	24.17117260295253	25.731115763565114	25.59279079574316	24.504920837739196
88-89	24.306669410157696	25.441940320255753	25.592061064160504	24.659329205426054
90-91	24.260539678237812	25.484058967333524	25.646614644443193	24.60878670998547
92-93	24.389219389629798	25.59830305207136	25.509472752923962	24.503004805374882
94-95	24.3732713283855	25.34920474908543	25.645705843371402	24.631818079157668
96-97	24.32145250353434	25.415506800554255	25.628985512036305	24.634055183875102
98-99	24.228982799326207	25.461112023064963	25.512726163025768	24.797179014583058
100-101	24.218068544405686	25.5145925743331	25.657977544800918	24.609361336460296
102-103	24.22178940103411	25.31860737579449	25.747006712828956	24.71259651034245
104-105	24.14833677320065	25.510182723328363	25.634337422065933	24.707143081405054
106-107	24.12520783466097	25.524809199725357	25.642190856183785	24.70779210942989
108-109	24.161491401733866	25.49050714842076	25.59544466515292	24.75255678469245
110-111	24.112881193114344	25.635780871657367	25.598710867184728	24.652627068043564
112-113	24.07902589735744	25.62053985900508	25.686694055568974	24.613740188068505
114-115	23.93295886287303	25.797480242529065	25.687562742622017	24.581998151975885
116-117	23.923157703687526	25.769743566156976	25.63225763629408	24.674841093861414
118-119	24.136078461145583	25.408380323976616	25.62686783493343	24.828673379944373
120-121	23.85818188049723	25.579760737963237	25.835319260201473	24.72673812133806
122-123	23.907375273719182	25.6717121811614	25.705265077522892	24.715647467596526
124-125	23.84076650638113	25.687602932509325	25.769150266316355	24.7024802947932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	96.5
2	126.0
3	147.5
4	150.0
5	145.5
6	146.5
7	184.0
8	243.0
9	308.5
10	331.5
11	339.5
12	402.5
13	479.0
14	554.5
15	641.0
16	703.5
17	811.0
18	953.0
19	1057.5
20	1178.5
21	1357.0
22	1651.5
23	2063.5
24	2460.5
25	3186.5
26	4481.0
27	5839.0
28	6849.5
29	8463.0
30	11114.5
31	13432.5
32	16360.5
33	19860.0
34	23193.5
35	26371.0
36	30263.5
37	34042.5
38	35972.0
39	38553.0
40	41625.5
41	43797.5
42	46038.5
43	50764.0
44	55715.5
45	59678.5
46	63176.5
47	66090.5
48	70485.5
49	74474.5
50	74378.0
51	73466.0
52	73462.5
53	73455.0
54	74019.0
55	72765.5
56	69154.0
57	63854.0
58	59444.5
59	55180.5
60	48912.0
61	42986.5
62	37020.0
63	29107.5
64	22010.5
65	17237.5
66	13423.0
67	10489.0
68	8353.0
69	6598.5
70	5426.0
71	4389.0
72	3461.0
73	2921.5
74	2364.5
75	1840.5
76	1309.5
77	784.0
78	483.5
79	297.0
80	171.5
81	99.5
82	63.5
83	39.0
84	25.5
85	20.0
86	16.0
87	14.5
88	11.5
89	10.5
90	11.0
91	8.5
92	8.0
93	9.0
94	7.5
95	8.0
96	7.5
97	7.0
98	7.5
99	6.0
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0367925286932153
2	0.02528109381764345
3	0.024620150188424016
4	0.03095419330177695
5	0.032606552374825536
6	0.04307149317079995
7	0.03822457322319075
8	0.03161513693099638
9	0.026823295619155467
10-11	0.03329503532192912
12-13	0.03246885578540482
14-15	0.031697754884648814
16-17	0.03368058577230713
18-19	0.04800103107206158
20-21	0.0578325675567007
22-23	0.05130574921815876
24-25	0.06223885841816361
26-27	0.045357256555183835
28-29	0.045467413826720406
30-31	0.04645882927054956
32-33	0.0536190519204268
34-35	0.03624174233553244
36-37	0.049185221741079733
38-39	0.058273196642847
40-41	0.04577034632344599
42-43	0.05463800668214009
44-45	0.05538156826501196
46-47	0.061743150696249034
48-49	0.04555003178037284
50-51	0.04769809857533601
52-53	0.05717162392748126
54-55	0.043539661574830385
56-57	0.04166698795870865
58-59	0.04235547090581223
60-61	0.030486024897746513
62-63	0.039904471614123485
64-65	0.03894059548817847
66-67	0.03235869851386825
68-69	0.028420576056435776
70-71	0.04662406517785442
72-73	0.030541103533514802
74-75	0.03629682097130072
76-77	0.030238171036789222
78-79	0.030403406944094085
80-81	0.02373889201613143
82-83	0.017101916406052922
84-85	0.016964219816632205
86-87	0.01627573686952863
88-89	0.020957420909832967
90-91	0.011456356239803567
92-93	0.018010713896229647
94-95	0.013604423034766736
96-97	0.010051851027712264
98-99	0.010575098067510984
100-101	0.010657716021163416
102-103	0.005893414027206643
104-105	0.006003571298743216
106-107	0.007738548325444237
108-109	0.012117299869023004
110-111	0.005618020848365211
112-113	0.007187761967761372
114-115	0.007352997875066232
116-117	0.007022526060456513
118-119	0.2477712430036363
120-121	0.36390454652106813
122-123	0.029825081268527075
124-125	0.004185976318389765
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1815586.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.83008562927765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.54966230451636	45.20814120545785
2	10.61200401608658	12.061621938648774
3	3.5215960755544535	6.003978195764632
4	1.759646033594012	4.000033390654659
5	1.0453308769957919	2.970312162529938
6	0.6928406283139752	2.3624515340715444
7	0.4784204157217432	1.9032071234582897
8	0.36239582149425087	1.6475988453768555
9	0.26639836050243226	1.3625497474967163
>10	1.5943697493886662	16.32203599819509
>50	0.08792891284180882	3.329186328792802
>100	0.029016121108340908	2.6069130515832857
>500	1.9534194081110624E-4	0.08736722488724866
>1k	1.9534194081110624E-4	0.13460325308232401
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.507863576828638E-5
5	0.0	0.0	0.0	0.0	5.507863576828638E-5
6	0.0	0.0	0.0	0.0	5.507863576828638E-5
7	0.0	0.0	0.0	0.0	5.507863576828638E-5
8	0.0	0.0	0.0	0.0	5.507863576828638E-5
9	0.0	0.0	0.0	0.0	5.507863576828638E-5
10-11	0.0	0.0	0.0	0.0	1.1015727153657276E-4
12-13	0.0	0.0	0.0	0.0	1.6523590730485916E-4
14-15	0.0	0.0	0.0	0.0	1.9277522518900233E-4
16-17	0.0	0.0	0.0	0.0	2.753931788414319E-4
18-19	0.0	0.0	0.0	2.753931788414319E-5	3.304718146097183E-4
20-21	0.0	0.0	0.0	5.507863576828638E-5	3.5801113249386146E-4
22-23	0.0	0.0	0.0	5.507863576828638E-5	4.4062908614629105E-4
24-25	0.0	0.0	0.0	2.478538609572887E-4	5.507863576828638E-4
26-27	0.0	0.0	0.0	2.753931788414319E-4	5.507863576828638E-4
28-29	0.0	0.0	0.0	9.638761259450117E-4	5.507863576828638E-4
30-31	0.0	5.507863576828638E-5	0.0	0.0032221001924447536	5.507863576828638E-4
32-33	0.0	5.507863576828638E-5	0.0	0.008206716729474671	5.507863576828638E-4
34-35	0.0	5.507863576828638E-5	0.0	0.016413433458949342	5.507863576828638E-4
36-37	0.0	8.261795365242957E-5	0.0	0.028200261513362627	5.507863576828638E-4
38-39	0.0	1.1015727153657276E-4	0.0	0.048083649025714006	5.507863576828638E-4
40-41	0.0	1.1015727153657276E-4	0.0	0.07198777694915029	5.507863576828638E-4
42-43	0.0	1.1015727153657276E-4	0.0	0.09214655764034312	5.507863576828638E-4
44-45	0.0	1.1015727153657276E-4	0.0	0.11797843781566944	5.78325675567007E-4
46-47	0.0	1.1015727153657276E-4	0.0	0.14675702500459908	6.609436292194365E-4
48-49	0.0	1.1015727153657276E-4	0.0	0.17743582512753459	7.711009007560093E-4
50-51	0.0	1.1015727153657276E-4	0.0	0.21018007409178086	7.711009007560093E-4
52-53	0.0	1.1015727153657276E-4	0.0	0.2435026487315941	7.986402186401525E-4
54-55	0.0	1.6523590730485913E-4	0.0	0.2813141321865227	8.261795365242957E-4
56-57	0.0	2.753931788414319E-4	0.0	0.3254871980726884	8.261795365242957E-4
58-59	0.0	2.753931788414319E-4	0.0	0.3721938812041952	8.261795365242957E-4
60-61	0.0	2.753931788414319E-4	0.0	0.41983690114376293	8.261795365242957E-4
62-63	0.0	2.753931788414319E-4	0.0	0.47072956059365956	8.261795365242957E-4
64-65	0.0	2.753931788414319E-4	0.0	0.5259183536334825	8.261795365242957E-4
66-67	0.0	2.753931788414319E-4	0.0	0.587551347058195	8.261795365242957E-4
68-69	0.0	2.753931788414319E-4	0.0	0.6607508539942476	8.261795365242957E-4
70-71	0.0	3.029324967255751E-4	0.0	0.7376130902088913	9.638761259450117E-4
72-73	0.0	3.304718146097183E-4	0.0	0.818523606152504	0.0012668086226705869
74-75	0.0	3.304718146097183E-4	0.0	0.9022706718381834	0.0013218872584388733
76-77	0.0	3.304718146097183E-4	0.0	0.9938113644850755	0.0013494265763230166
78-79	0.0	3.304718146097183E-4	0.0	1.1033352317103127	0.0013769658942071597
80-81	0.0	3.304718146097183E-4	0.0	1.224535769718427	0.0014871231657437325
82-83	0.0	3.304718146097183E-4	0.0	1.3517674183431687	0.0016523590730485914
84-85	0.0	3.304718146097183E-4	0.0	1.4824690210213123	0.0016523590730485914
86-87	0.0	3.8555045037800466E-4	0.0	1.6203583856672172	0.0016798983909327345
88-89	0.0	3.8555045037800466E-4	0.0	1.778048519871821	0.0017349770267010211
90-91	0.0	3.8555045037800466E-4	0.0	1.9541899970588008	0.0017625163445851642
92-93	0.0	3.8555045037800466E-4	0.0	2.141842909121353	0.001872673616121737
94-95	0.0	3.8555045037800466E-4	0.0	2.3343427411315134	0.001872673616121737
96-97	0.0	4.1308976826214786E-4	0.0	2.538051075520521	0.00198283088765831
98-99	0.0	4.4062908614629105E-4	0.0	2.7626066735478245	0.00198283088765831
100-101	0.0	4.4062908614629105E-4	0.0	3.018171543512673	0.00198283088765831
102-103	0.0	4.4062908614629105E-4	0.0	3.291444194877026	0.0021205274770790257
104-105	0.0	4.4062908614629105E-4	0.0	3.5823420096872303	0.0021480667949631688
106-107	0.0	4.4062908614629105E-4	0.0	3.8706235892984413	0.0021480667949631688
108-109	0.0	4.4062908614629105E-4	0.0	4.178540702561047	0.0021756061128473123
110-111	0.0	4.4062908614629105E-4	0.0	4.51994562637077	0.0022306847486155985
112-113	0.0	4.4062908614629105E-4	0.0	4.8876505987598495	0.0022582240664997416
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1815	0.0	67.88536	1
GTATCAA	3660	0.0	56.92058	1
TCAACGC	4590	0.0	44.462738	4
CAACGCA	4705	0.0	43.123055	5
ATCAACG	5025	0.0	40.966686	3
AACGCAG	4955	0.0	40.703793	6
TATCAAC	5385	0.0	38.669907	2
GTACATG	8705	0.0	38.15468	1
TACATGG	9220	0.0	35.555885	2
ACATGGG	9575	0.0	34.424034	3
ACGCAGA	5980	0.0	33.726044	7
CATGGGG	7915	0.0	32.62523	4
CGCAGAG	6430	0.0	31.271498	8
GCAGAGT	7110	0.0	27.527655	9
ATGGGGG	4420	0.0	27.326784	5
TACCGTA	200	6.12672E-8	26.771912	7
AGAGTAC	6245	0.0	26.054323	10-11
CAGAGTA	6765	0.0	23.392076	10-11
ATGGGGA	3300	0.0	22.718067	5
GAGTACA	6115	0.0	20.343805	1
>>END_MODULE