FastQCFastQC Report
Mon 15 Aug 2016
SRR2921528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815586
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.1452423625209712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.1402852853018254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.077.804541
GTATCAA49250.060.6796461
TCAACGC64900.045.2308584
CAACGCA66550.043.9932755
ATCAACG69700.042.2037163
AACGCAG69600.041.970666
TATCAAC71700.041.276772
GTACATG85000.036.2813261
ACGCAGA81300.035.7051627
TACATGG87900.034.7533762
CGCAGAG85150.034.090788
ACATGGG89500.033.5323523
GCAGAGT93500.030.664599
CATGGGG75000.029.9304124
AGAGTAC86200.026.74066410-11
CAGAGTA91150.025.97371910-11
TATACCG1859.254163E-725.732835
GTATTAG7300.025.3308851
ATACCGT2351.0426447E-825.3166186
GAGTACT51000.024.96004512-13
Produced by FastQC (version 0.11.5)