##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921528_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1815586
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35489037699123	33.0	31.0	34.0	28.0	34.0
2	31.431775746232898	33.0	31.0	34.0	28.0	34.0
3	31.524710479151086	33.0	31.0	34.0	28.0	34.0
4	35.027139997774825	37.0	35.0	37.0	32.0	37.0
5	34.70263209784609	37.0	35.0	37.0	32.0	37.0
6	34.67740387951879	37.0	35.0	37.0	31.0	37.0
7	34.52949075394941	37.0	35.0	37.0	30.0	37.0
8	34.49734135425147	37.0	35.0	37.0	30.0	37.0
9	36.09299807334932	39.0	37.0	39.0	31.0	39.0
10-11	35.974071456818905	39.0	37.0	39.0	30.0	39.0
12-13	35.8802414206763	39.0	37.0	39.0	30.0	39.0
14-15	37.08475087382256	40.0	37.0	41.0	31.0	41.0
16-17	37.06463285132183	40.0	37.0	41.0	30.5	41.0
18-19	37.086337138532684	40.0	37.0	41.0	30.0	41.0
20-21	37.06824656061459	40.0	37.0	41.0	30.0	41.0
22-23	37.00717068759067	40.0	37.0	41.0	30.0	41.0
24-25	36.9466590951902	40.0	37.0	41.0	30.0	41.0
26-27	36.824875825215656	40.0	37.0	41.0	30.0	41.0
28-29	36.642355966613536	40.0	36.0	41.0	30.0	41.0
30-31	36.60266024302898	40.0	36.0	41.0	30.0	41.0
32-33	36.48681527616979	40.0	36.0	41.0	30.0	41.0
34-35	36.36622914034367	40.0	36.0	41.0	29.5	41.0
36-37	36.240602758558396	40.0	35.0	41.0	29.0	41.0
38-39	36.076761166918004	40.0	35.0	41.0	28.5	41.0
40-41	35.885863021635984	40.0	35.0	41.0	27.5	41.0
42-43	35.72075792609108	40.0	35.0	41.0	27.0	41.0
44-45	35.515721645793704	39.5	35.0	41.0	26.5	41.0
46-47	35.253511263030234	39.0	35.0	41.0	25.5	41.0
48-49	35.00544975561609	39.0	35.0	41.0	24.0	41.0
50-51	34.79675349997191	39.0	34.5	41.0	24.0	41.0
52-53	34.6041267117063	39.0	34.0	41.0	23.0	41.0
54-55	34.38947783250146	38.0	34.0	40.5	23.0	41.0
56-57	34.13373285539765	38.0	34.0	40.0	22.5	41.0
58-59	33.99410686136597	38.0	34.0	40.0	22.5	41.0
60-61	33.87732500691237	37.0	34.0	40.0	22.0	41.0
62-63	33.60739728109822	37.0	33.5	40.0	22.0	41.0
64-65	33.31105191381736	36.0	33.0	39.5	21.5	41.0
66-67	33.007977864997855	36.0	33.0	39.0	21.0	41.0
68-69	32.6889500139349	35.0	33.0	39.0	20.0	41.0
70-71	32.38107365886276	35.0	33.0	38.5	20.0	40.0
72-73	32.061126820762	35.0	32.5	37.0	19.5	39.5
74-75	31.7653024973755	35.0	32.0	37.0	18.5	39.0
76-77	31.466792539708944	35.0	32.0	36.0	18.0	39.0
78-79	31.18647147532532	35.0	32.0	36.0	17.0	37.5
80-81	30.959874938449623	35.0	32.0	35.5	15.5	37.0
82-83	30.76238140192753	35.0	32.0	35.0	14.5	37.0
84-85	30.56450506888685	35.0	31.5	35.0	12.5	36.0
86-87	30.417443734419628	35.0	31.0	35.0	11.0	36.0
88-89	30.31029485796872	35.0	31.0	35.0	10.0	36.0
90-91	30.210816232334906	35.0	31.0	35.0	8.0	35.5
92-93	30.269814263824465	35.0	32.0	35.0	8.5	35.0
94-95	30.238081258612922	35.0	32.0	35.0	7.0	35.0
96-97	30.214585814166888	35.0	32.0	35.0	7.0	35.0
98-99	30.172076949260457	35.0	32.0	35.0	7.0	35.0
100-101	30.125357873435902	35.0	32.0	35.0	4.0	35.0
102-103	29.249801992304413	34.0	29.5	35.0	2.0	35.0
104-105	29.95577653716211	35.0	31.0	35.0	2.0	35.0
106-107	30.060831599274284	35.0	32.0	35.0	2.0	35.0
108-109	30.06547224973094	35.0	32.0	35.0	2.0	35.0
110-111	30.029398497234503	35.0	32.0	35.0	2.0	35.0
112-113	29.98868684821319	35.0	32.0	35.0	2.0	35.0
114-115	29.925260494407866	35.0	32.0	35.0	2.0	35.0
116-117	29.850888087923128	35.0	32.0	35.0	2.0	35.0
118-119	29.762681029706112	35.0	32.0	35.0	2.0	35.0
120-121	29.643004517549706	35.0	32.0	35.0	2.0	35.0
122-123	29.528075783796524	35.0	32.0	35.0	2.0	35.0
124-125	28.624851976166376	34.0	29.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27893.0
3	10402.0
4	5726.0
5	4822.0
6	5161.0
7	6033.0
8	7045.0
9	8084.0
10	9001.0
11	10028.0
12	10460.0
13	11920.0
14	15210.0
15	12951.0
16	7194.0
17	6140.0
18	5545.0
19	4891.0
20	4864.0
21	5111.0
22	6108.0
23	8013.0
24	11135.0
25	15862.0
26	19994.0
27	22158.0
28	25809.0
29	31337.0
30	36246.0
31	43750.0
32	54454.0
33	70319.0
34	97205.0
35	155128.0
36	314523.0
37	539304.0
38	185378.0
39	382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.6404157845015	17.876273888775216	12.45853433095436	25.024775995768916
2	16.733891289440418	19.825507902542196	37.61301640545177	25.827584402565606
3	18.786287183775734	24.571302336317565	29.3104312621095	27.331979217797198
4	13.210541979431897	15.667746877858976	35.766729495276834	35.3549816474323
5	14.152634116107707	37.30586047792851	33.67195096756422	14.86955443839956
6	33.3360326964489	35.40666456226521	16.788826619907088	14.46847612137879
7	29.15609926999579	31.614878160028027	20.92493273957601	18.30408983040017
8	27.772165592395236	33.66753071064955	19.296015299417128	19.26428839753808
9	27.686674201714556	14.21215786586293	18.97355903225309	39.12760890016943
10-11	26.196918858462315	24.89609402777858	27.10844916463482	21.798537949124285
12-13	26.522935711596567	22.693574244208957	27.424038081000894	23.35945196319358
14-15	24.101536218497138	23.625641995902573	24.83655594690144	27.436265838698848
16-17	23.917768143555964	26.64542620965532	25.33478094539313	24.102024701395585
18-19	23.928563363027912	25.60012955230096	27.378003539553937	23.093303545117195
20-21	24.928898596150084	24.586295318021445	27.18450559529351	23.300300490534962
22-23	24.776257194265906	24.25197123643187	26.99329053886384	23.978481030438388
24-25	24.240447826474952	24.791802774787836	27.02630793248229	23.94144146625492
26-27	23.95751855760735	24.798752690825523	27.164530898498867	24.07919785306826
28-29	24.066411527269572	24.8464825605015	27.200026441218554	23.887079471010374
30-31	24.314347996383955	24.792328079491284	27.008780642591653	23.884543281533105
32-33	23.935517099866967	24.866927587565037	27.028145854965807	24.16940945760219
34-35	24.19168957001207	24.86614695491553	27.077400912387752	23.864762562684653
36-37	24.429281722913124	24.77254999603381	26.77795503221428	24.020213248838786
38-39	24.313331006723388	25.037095839894715	26.691789279653467	23.957783873728435
40-41	24.311390802055616	24.854985068164797	26.88256386344401	23.951060266335578
42-43	24.256216772706654	24.928673981915594	26.579218218919305	24.235891026458447
44-45	24.27374811773173	24.95826439372301	26.497792509377945	24.270194979167314
46-47	24.44716449910016	25.050362938165826	26.212470177483567	24.290002385250446
48-49	24.524724726103333	25.35153113573293	25.783265275205036	24.340478862958705
50-51	24.465853516117768	25.485174563687185	25.652417251777365	24.396554668417682
52-53	24.565460324350884	25.34222332156528	25.76704657355243	24.3252697805314
54-55	24.437245099057257	25.352306258757217	25.818871215236587	24.391577426948942
56-57	24.375348436470528	25.40297435287222	25.723067651213167	24.498609559444088
58-59	24.36599270037407	25.23199352613358	25.860099592436175	24.541914181056175
60-61	24.528069579128463	25.36081667774866	25.900437238363494	24.21067650475938
62-63	24.39508776336999	25.37185534331338	25.915342586196065	24.31771430712056
64-65	24.372152517242927	25.107646932933473	25.998156237269733	24.522044312553863
66-67	24.327781999400127	25.37257156108923	25.87122488039068	24.428421559119958
68-69	24.418543795009214	25.353820879866724	25.778929298627578	24.448706026496485
70-71	24.30278862515486	25.152409549951827	26.028829876954113	24.5159719479392
72-73	24.508975445191556	25.41547172181133	25.642433203362998	24.433119629634117
74-75	24.342115410887967	25.56360202898934	25.600868842850282	24.49341371727241
76-77	24.39639598945998	25.199677310339265	25.728193177520904	24.675733522679856
78-79	24.28097977429788	25.265943893186655	25.735672278780434	24.71740405373503
80-81	24.32339450804798	25.491285440256316	25.50604934449367	24.67927070720203
82-83	24.46049704173302	25.404382785039942	25.536611067074798	24.598509106152235
84-85	24.228183945212496	25.4189555615843	25.524278008293038	24.828582484910164
86-87	24.156996224182585	25.527379151946285	25.61631426346772	24.699310360403413
88-89	24.288829594274443	25.296663589920726	25.55982590631102	24.854680909493812
90-91	24.193942865956437	25.287488019037337	25.671815267326952	24.846753847679274
92-93	24.23518090711896	25.482273933577236	25.556139428971797	24.726405730332004
94-95	24.191237587849685	25.215386301756194	25.650345174415946	24.943030935978175
96-97	24.213637462731537	25.269905477314502	25.615008843084407	24.901448216869554
98-99	24.138423902132324	25.256292364835296	25.546479351889108	25.058804381143275
100-101	24.11924029672914	25.280311205342315	25.650506529440182	24.949941968488364
102-103	24.10695032234309	25.09054496072644	25.77954315932856	25.02296155760191
104-105	24.037990974017873	25.346942900900093	25.606425428516584	25.008640696565447
106-107	24.049635915344982	25.26238853083077	25.580441076559264	25.107534477264988
108-109	24.08746719140224	25.20733215866457	25.574964736524475	25.130235913408715
110-111	24.10292734190657	25.295636857144743	25.544759447711247	25.05667635323744
112-113	23.959695499137943	25.290399519673024	25.661716507934763	25.08818847325427
114-115	23.79413553182958	25.409408970534454	25.68439542087661	25.112060076759363
116-117	23.806566397955333	25.350669666832832	25.522141832212863	25.320622102998975
118-119	23.815766606781715	25.029311310213505	25.711052312529485	25.443869770475295
120-121	23.607872816113133	25.239863333393924	25.792351803594908	25.359912046898035
122-123	23.695309666444423	25.29422505107887	25.655350413877258	25.355114868599443
124-125	23.553056190267625	25.265690702168303	25.67891236290564	25.50234074465843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	30.5
2	60.0
3	95.5
4	83.0
5	69.0
6	106.5
7	254.0
8	441.5
9	519.5
10	459.0
11	412.0
12	481.0
13	585.5
14	740.0
15	874.0
16	980.5
17	1143.0
18	1339.5
19	1471.5
20	1666.5
21	1987.5
22	2348.0
23	2715.0
24	3118.5
25	3937.0
26	5161.5
27	6380.5
28	7238.5
29	8731.0
30	11286.0
31	13498.0
32	16290.0
33	19472.5
34	22685.5
35	25998.0
36	29576.5
37	33160.5
38	35373.5
39	37816.5
40	40652.0
41	42998.5
42	45156.5
43	49639.5
44	54975.5
45	59244.0
46	62776.5
47	65952.0
48	70604.0
49	74373.5
50	74463.5
51	73767.0
52	73736.0
53	73713.0
54	74287.5
55	73477.0
56	69530.0
57	63941.5
58	59980.5
59	55730.0
60	49577.0
61	43382.0
62	36855.5
63	28859.5
64	21745.5
65	17149.0
66	13316.0
67	10164.5
68	8044.0
69	6328.0
70	5160.0
71	4226.5
72	3366.5
73	2934.0
74	2324.5
75	1747.5
76	1209.0
77	685.0
78	395.5
79	240.5
80	132.5
81	58.5
82	35.0
83	18.0
84	13.5
85	7.0
86	3.0
87	2.0
88	1.5
89	2.0
90	1.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12882892906202184
2	0.0017074377088168778
3	0.02087480295618054
4	0.06201854387509046
5	0.025391251089180025
6	0.01889197206852223
7	0.01398997348514474
8	0.0050672344906823475
9	0.003855504503780047
10-11	0.006747132881615082
12-13	0.004819380629725058
14-15	0.003111942920908181
16-17	0.010051851027712264
18-19	0.005673099484133498
20-21	0.012172378504791291
22-23	0.00906043558388311
24-25	0.013218872584388731
26-27	0.00870242445138925
28-29	0.013136254630736303
30-31	0.01889197206852223
32-33	0.012172378504791291
34-35	0.019222443883131945
36-37	0.013329029855925306
38-39	0.01958045501562581
40-41	0.009335828762724542
42-43	0.008509649226200245
44-45	0.015945265054918906
46-47	0.014485681207059318
48-49	0.019194904565247806
50-51	0.01432044529975446
52-53	0.019855848194467243
54-55	0.0163858941410652
56-57	0.01839626434660765
58-59	0.015835107783382336
60-61	0.01812087116776622
62-63	0.020048623419656245
64-65	0.015229242789931185
66-67	0.010822951928468274
68-69	0.022141611578851125
70-71	0.013604423034766736
72-73	0.016523590730485916
74-75	0.016413433458949342
76-77	0.01208976055113886
78-79	0.027236385387417614
80-81	0.019167365247363663
82-83	0.009087974901767253
84-85	0.011483895557687711
86-87	0.01197960327960229
88-89	0.019635533651394095
90-91	0.011346198968266996
92-93	0.006829750835267511
94-95	0.00699498674257237
96-97	0.0023683813380363143
98-99	0.0017900556624693075
100-101	0.010630176703279271
102-103	0.002836549742066749
104-105	0.0027263924705301762
106-107	3.029324967255751E-4
108-109	0.017762860035272356
110-111	7.711009007560093E-4
112-113	0.008592267179852675
114-115	0.002313302702268028
116-117	0.007215301285645515
118-119	0.008840121040809964
120-121	0.004020740411084906
122-123	6.334043113352934E-4
124-125	0.025253554499759308
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1815586.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.49950603197114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02338872682337	43.85759039311134
2	10.68981997923365	11.86559456836327
3	3.7283992612305394	6.207729518647833
4	1.8184204448312844	4.036857457862941
5	1.0640767637839479	2.952786738505377
6	0.7257064236204791	2.416580882109899
7	0.4932230929905904	1.9161546617177112
8	0.3827139007556099	1.6992345954804138
9	0.2913443831797754	1.455252241650017
>10	1.6561180163629607	16.70989282911633
>50	0.09603419887095642	3.629229395993352
>100	0.030356776016691837	2.8379039648602986
>500	9.950807502582286E-5	0.05108450334366081
>1k	2.985242250774686E-4	0.36410824923757373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2637	0.1452423625209712	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2547	0.1402852853018254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.507863576828638E-5
7	0.0	0.0	0.0	0.0	5.507863576828638E-5
8	0.0	0.0	0.0	0.0	5.507863576828638E-5
9	0.0	0.0	0.0	0.0	5.507863576828638E-5
10-11	0.0	0.0	0.0	0.0	5.507863576828638E-5
12-13	0.0	0.0	0.0	0.0	1.3769658942071596E-4
14-15	0.0	0.0	0.0	0.0	1.6523590730485916E-4
16-17	0.0	0.0	0.0	0.0	1.6523590730485916E-4
18-19	0.0	0.0	0.0	5.507863576828638E-5	1.6523590730485916E-4
20-21	0.0	0.0	0.0	5.507863576828638E-5	3.0293249672557507E-4
22-23	0.0	0.0	0.0	5.507863576828638E-5	3.8555045037800466E-4
24-25	0.0	0.0	0.0	2.478538609572887E-4	4.1308976826214786E-4
26-27	0.0	0.0	0.0	2.753931788414319E-4	5.232470397987206E-4
28-29	0.0	0.0	0.0	9.91415443829155E-4	5.507863576828638E-4
30-31	0.0	5.507863576828638E-5	0.0	0.0034424147355178987	5.507863576828638E-4
32-33	0.0	5.507863576828638E-5	0.0	0.00853718854408439	6.058649934511502E-4
34-35	0.0	5.507863576828638E-5	0.0	0.016936680498748063	6.058649934511502E-4
36-37	0.0	8.261795365242957E-5	0.0	0.029191676957191784	6.058649934511502E-4
38-39	0.0	1.1015727153657276E-4	0.0	0.04940553628415288	6.334043113352934E-4
40-41	0.0	1.1015727153657276E-4	0.0	0.07435615828718661	6.609436292194366E-4
42-43	0.0	1.1015727153657276E-4	0.0	0.09454247829626358	6.884829471035798E-4
44-45	0.0	1.3769658942071596E-4	0.0	0.12100776278292519	7.160222649877229E-4
46-47	0.0	1.6523590730485916E-4	0.0	0.15072268677991568	7.435615828718661E-4
48-49	0.0	1.6523590730485916E-4	0.0	0.1819247339426499	8.261795365242957E-4
50-51	0.0	1.6523590730485916E-4	0.0	0.21571547698649363	8.261795365242957E-4
52-53	0.0	1.6523590730485916E-4	0.0	0.24942360207668487	9.638761259450117E-4
54-55	0.0	1.6523590730485916E-4	0.0	0.2883917368827475	9.91415443829155E-4
56-57	0.0	1.6523590730485916E-4	0.0	0.3328401959477546	9.91415443829155E-4
58-59	0.0	2.2031454307314553E-4	0.0	0.38029044066213336	9.91415443829155E-4
60-61	0.0	2.2031454307314553E-4	0.0	0.42925534786013997	9.91415443829155E-4
62-63	0.0	2.2031454307314553E-4	0.0	0.4816902091115486	9.91415443829155E-4
64-65	0.0	2.2031454307314553E-4	0.0	0.5374297885090544	0.0010464940795974413
66-67	0.0	2.2031454307314553E-4	0.0	0.6004122085100898	0.0010464940795974413
68-69	0.0	2.2031454307314553E-4	0.0	0.6750988386118861	0.0010464940795974413
70-71	0.0	2.2031454307314553E-4	0.0	0.7534206586743895	0.0010464940795974413
72-73	0.0	2.2031454307314553E-4	0.0	0.8354052080154837	0.0011841906690181572
74-75	0.0	2.2031454307314553E-4	0.0	0.9203364643701812	0.0012117299869023005
76-77	0.0	2.2031454307314553E-4	0.0	1.0129787297324389	0.0012117299869023005
78-79	0.0	2.2031454307314553E-4	0.0	1.1244303492095664	0.0012117299869023005
80-81	0.0	2.2031454307314553E-4	0.0	1.247448482198034	0.0012392693047864438
82-83	0.0	2.2031454307314553E-4	0.0	1.3765252651210131	0.0012668086226705869
84-85	0.0	2.2031454307314553E-4	0.0	1.5097054064087296	0.0012668086226705869
86-87	0.0	2.2031454307314553E-4	0.0	1.6504863994324697	0.0013769658942071597
88-89	0.0	2.2031454307314553E-4	0.0	1.811866802233549	0.0014871231657437322
90-91	0.0	2.2031454307314553E-4	0.0	1.9918362446064246	0.0014871231657437322
92-93	0.0	2.2031454307314553E-4	0.0	2.184143301391397	0.0015697411193961617
94-95	0.0	2.2031454307314553E-4	0.0	2.3812421994882094	0.0016798983909327345
96-97	0.0	2.2031454307314553E-4	0.0	2.587153679307948	0.00190021293400588
98-99	0.0	2.2031454307314553E-4	0.0	2.815509703203263	0.0021205274770790257
100-101	0.0	2.2031454307314553E-4	0.0	3.0757011785726482	0.0021480667949631688
102-103	0.0	2.753931788414319E-4	0.0	3.354509232831714	0.0023683813380363143
104-105	0.0	2.753931788414319E-4	0.0	3.6503090462252956	0.002423459973804601
106-107	0.0	2.753931788414319E-4	0.0	3.9446492757710185	0.002450999291688744
108-109	0.0	2.753931788414319E-4	0.0	4.258569960332366	0.0025336172453411738
110-111	0.0	2.753931788414319E-4	0.0	4.607217724745619	0.0026713138347618896
112-113	0.0	2.753931788414319E-4	0.0	4.983404807043016	0.0026988531526460327
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2415	0.0	77.80454	1
GTATCAA	4925	0.0	60.679646	1
TCAACGC	6490	0.0	45.230858	4
CAACGCA	6655	0.0	43.993275	5
ATCAACG	6970	0.0	42.203716	3
AACGCAG	6960	0.0	41.97066	6
TATCAAC	7170	0.0	41.27677	2
GTACATG	8500	0.0	36.281326	1
ACGCAGA	8130	0.0	35.705162	7
TACATGG	8790	0.0	34.753376	2
CGCAGAG	8515	0.0	34.09078	8
ACATGGG	8950	0.0	33.532352	3
GCAGAGT	9350	0.0	30.66459	9
CATGGGG	7500	0.0	29.930412	4
AGAGTAC	8620	0.0	26.740664	10-11
CAGAGTA	9115	0.0	25.973719	10-11
TATACCG	185	9.254163E-7	25.73283	5
GTATTAG	730	0.0	25.330885	1
ATACCGT	235	1.0426447E-8	25.316618	6
GAGTACT	5100	0.0	24.960045	12-13
>>END_MODULE