FastQCFastQC Report
Mon 15 Aug 2016
SRR2921527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940660
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.11748580379870766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.069.499411
GTATCAA45900.061.7164541
TCAACGC58550.046.8467944
CAACGCA60150.045.5017435
AACGCAG63000.043.534416
ATCAACG64200.043.1840253
TATCAAC66100.042.122742
GTACATG105150.039.1088871
TACATGG109850.036.9365732
ACGCAGA74750.036.452447
ACATGGG113250.035.7751273
CGCAGAG80000.033.982378
CATGGGG93600.032.864014
GCAGAGT86150.030.8659559
ATGGGGG51200.029.8654485
AGAGTAC77550.027.193310-11
CAGAGTA81800.025.6713610-11
GTGGTAT10150.024.6257741
GTATCGC1802.179784E-523.1365076
CATGGGT18650.022.9699214
Produced by FastQC (version 0.11.5)