##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921527_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1940660
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.89116795317057	31.0	30.0	34.0	25.0	34.0
2	29.913185720321952	31.0	30.0	34.0	25.0	34.0
3	29.983137695423206	31.0	30.0	34.0	25.0	34.0
4	33.35343491389527	37.0	35.0	37.0	27.0	37.0
5	33.3481583584966	37.0	35.0	37.0	27.0	37.0
6	33.361246173982046	37.0	35.0	37.0	27.0	37.0
7	33.166814898024384	37.0	35.0	37.0	26.0	37.0
8	33.13528181134253	37.0	35.0	37.0	26.0	37.0
9	34.56747137571754	39.0	35.0	39.0	25.0	39.0
10-11	34.459877567425515	39.0	35.0	39.0	25.0	39.0
12-13	34.332234394484345	39.0	35.0	39.0	24.5	39.0
14-15	35.48910241876475	40.0	36.0	41.0	25.0	41.0
16-17	35.44885708985603	40.0	36.0	41.0	25.0	41.0
18-19	35.48117418816279	40.0	36.0	41.0	25.0	41.0
20-21	35.506066492842635	40.0	36.0	41.0	25.0	41.0
22-23	35.48501978708275	40.0	35.5	41.0	25.0	41.0
24-25	35.457311687776325	40.0	35.0	41.0	25.0	41.0
26-27	35.38811151876166	40.0	35.0	41.0	25.0	41.0
28-29	35.34809884266178	40.0	35.0	41.0	25.0	41.0
30-31	35.250993734090464	40.0	35.0	41.0	25.0	41.0
32-33	35.13775983428319	40.0	35.0	41.0	24.0	41.0
34-35	34.97282599734111	40.0	35.0	41.0	24.0	41.0
36-37	34.83078128059526	39.0	34.0	41.0	23.0	41.0
38-39	34.708210608761966	39.0	34.0	41.0	22.5	41.0
40-41	34.552299217791884	39.0	34.0	41.0	21.5	41.0
42-43	34.35605154947286	39.0	34.0	41.0	20.0	41.0
44-45	34.15330428823184	39.0	33.0	41.0	18.5	41.0
46-47	33.89065111869158	38.5	33.0	41.0	15.5	41.0
48-49	33.72708460008451	38.0	33.0	41.0	14.5	41.0
50-51	33.52753831170838	38.0	33.0	40.0	13.0	41.0
52-53	33.33684648521637	38.0	33.0	40.0	10.5	41.0
54-55	33.138449548091884	38.0	33.0	40.0	8.5	41.0
56-57	32.895976111219895	37.0	32.0	40.0	8.0	41.0
58-59	32.65428797419435	37.0	32.0	40.0	7.0	41.0
60-61	32.34970911957788	36.0	32.0	40.0	4.0	41.0
62-63	32.03601867405934	36.0	31.0	39.0	2.0	41.0
64-65	31.72880154174353	35.0	31.0	39.0	2.0	41.0
66-67	31.676603578164126	35.0	31.0	39.0	2.0	41.0
68-69	31.430825080127377	35.0	31.0	38.5	2.0	40.5
70-71	31.12992667443035	35.0	31.0	37.0	2.0	40.0
72-73	30.80121685405996	35.0	31.0	37.0	2.0	39.0
74-75	30.499741325116197	35.0	31.0	36.0	2.0	39.0
76-77	30.216306050518895	35.0	31.0	36.0	2.0	38.5
78-79	29.943073232817703	35.0	30.0	35.5	2.0	37.0
80-81	29.70569419681964	35.0	30.0	35.0	2.0	37.0
82-83	29.502239186668454	35.0	30.0	35.0	2.0	36.5
84-85	29.305811940267745	35.0	30.0	35.0	2.0	36.0
86-87	29.15001339750394	35.0	30.0	35.0	2.0	36.0
88-89	29.023315263879297	34.0	30.0	35.0	2.0	35.5
90-91	28.908156503457587	34.0	30.0	35.0	2.0	35.0
92-93	28.80979666711325	34.0	29.0	35.0	2.0	35.0
94-95	28.74086161409005	34.0	29.0	35.0	2.0	35.0
96-97	28.64127255675904	34.0	29.0	35.0	2.0	35.0
98-99	28.580090278565024	34.0	29.0	35.0	2.0	35.0
100-101	28.51805236362887	34.0	29.0	35.0	2.0	35.0
102-103	27.55923474488061	32.5	27.0	34.5	2.0	35.0
104-105	28.36203301969433	34.0	29.0	35.0	2.0	35.0
106-107	28.678249667638845	34.5	30.0	35.0	2.0	35.0
108-109	28.713026238496184	35.0	30.0	35.0	2.0	35.0
110-111	28.65144873393588	35.0	30.0	35.0	2.0	35.0
112-113	28.579118701884923	35.0	30.0	35.0	2.0	35.0
114-115	28.459607813836527	35.0	29.5	35.0	2.0	35.0
116-117	28.39523358032834	35.0	29.0	35.0	2.0	35.0
118-119	28.237253305576452	35.0	29.0	35.0	2.0	35.0
120-121	28.067411602238415	34.5	29.0	35.0	2.0	35.0
122-123	27.93798939536034	34.0	29.0	35.0	2.0	35.0
124-125	27.10783058340977	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75309.0
3	13120.0
4	8481.0
5	7433.0
6	7740.0
7	9042.0
8	9817.0
9	10497.0
10	11224.0
11	11983.0
12	12171.0
13	12921.0
14	15183.0
15	13083.0
16	9001.0
17	7914.0
18	7326.0
19	6423.0
20	6368.0
21	6660.0
22	7945.0
23	10693.0
24	15327.0
25	21407.0
26	25284.0
27	26376.0
28	30599.0
29	36642.0
30	40808.0
31	49119.0
32	61915.0
33	80384.0
34	113076.0
35	176875.0
36	337028.0
37	501600.0
38	143686.0
39	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.884992154525946	17.141536063407102	12.37626110067568	24.597210681391278
2	16.71416238225933	19.53504194209285	38.30520442743567	25.445591248212146
3	18.917337830552295	24.65247747353056	28.926502013749367	27.503682682167778
4	13.436480867433065	15.361000046905549	35.6544019826099	35.54811710305149
5	14.390294065646087	37.38441419601662	33.170619311559896	15.054672426777401
6	33.215725312755026	34.693730258684376	17.49768143451541	14.592862994045191
7	29.548070682613332	31.689488489769747	20.617583637776093	18.14485718984083
8	27.998263900522737	33.2381605799232	19.572511277172303	19.191064242381756
9	28.18350742364381	14.514673326168698	18.854947077061055	38.44687217312644
10-11	26.42794420828618	24.968511037954023	26.80931696981209	21.794227783947704
12-13	26.638374271480703	22.801143001768835	26.91252665298783	23.647956073762636
14-15	24.181642735197816	23.794990061773706	24.467804889112486	27.555562313915992
16-17	24.182642333806108	26.719762386302158	24.88768427935589	24.209911000535836
18-19	24.22922546584155	25.915681822107	26.51402531901385	23.341067393037594
20-21	25.49908004332059	24.838263185723488	26.150229428456452	23.51242734249947
22-23	25.378512717109597	24.41853630922583	25.958855060575953	24.24409591308862
24-25	24.660842645734153	24.840379099316863	26.098603771502997	24.400174483445987
26-27	24.277996020085165	24.94558549083898	26.216142369595925	24.56027611947993
28-29	24.402743791708563	25.03873617103872	26.225090212553383	24.333429824699333
30-31	24.80616458359701	24.91512329966276	25.994863706230596	24.28384841050963
32-33	24.45017940883121	25.02260172322189	25.961858963700358	24.565359904246545
34-35	24.674814538740023	25.079939976070847	26.13876126157474	24.106484223614395
36-37	24.818873362152743	24.8568180587885	25.97791371190386	24.346394867154896
38-39	24.707475248698888	25.139520926592198	25.833258571565576	24.319745253143346
40-41	24.75320491422329	24.94859429200092	25.993283039042048	24.304917754733747
42-43	24.616274597352568	25.000386690026183	25.83097108692895	24.552367625692302
44-45	24.524925278558747	25.088049364358607	25.82936030609367	24.557665050988973
46-47	24.63780632680084	25.07038143126441	25.701777633863937	24.590034608070816
48-49	24.74824909845003	25.265850911087455	25.46613806667474	24.519761923787772
50-51	24.73951781538322	25.448787217632674	25.326653166102307	24.4850418008818
52-53	24.83350047306129	25.251392759455427	25.464386347992647	24.45072041949064
54-55	24.568875966294712	25.201172312998292	25.66599905658189	24.563952664125107
56-57	24.592730941313352	25.3500727648209	25.340277996155297	24.716918297710446
58-59	24.674686633843415	25.227841148251855	25.45147435267905	24.64599786522568
60-61	24.805244945750026	25.30068283469957	25.60983215988033	24.284240059670072
62-63	24.650162102084362	25.412575451316023	25.474745943839324	24.462516502760298
64-65	24.607815795212083	25.116620382804573	25.632977135754942	24.642586686228405
66-67	24.594197265747827	25.340544396803683	25.482633344020716	24.582624993427775
68-69	24.527954203619874	25.311638882388536	25.51054964109468	24.649857272896902
70-71	24.509339175457306	25.19310276740464	25.621801866314243	24.675756190823815
72-73	24.681473012704995	25.490019711096355	25.32545989121784	24.503047384980807
74-75	24.46938398358877	25.634732664172198	25.37770798005971	24.51817537217932
76-77	24.559934908072083	25.192993597249107	25.418841144098366	24.828230350580444
78-79	24.539341920342718	25.284307123083817	25.49157265363023	24.68477830294324
80-81	24.595661994843255	25.531504205156487	25.27933655554711	24.59349724445315
82-83	24.696620643093116	25.430008606872097	25.389061541712405	24.48430920832238
84-85	24.523284693314313	25.510910890997256	25.210005210510282	24.755799205178146
86-87	24.276585870293747	25.68291845126384	25.39557049688185	24.644925181560566
88-89	24.418843477581873	25.411290407789487	25.359079416628504	24.810786698000133
90-91	24.44415813979855	25.32043072816147	25.416337058443084	24.8190740735969
92-93	24.5493529715994	25.466250337899908	25.346629259954746	24.637767430545946
94-95	24.454298805305484	25.264762580136114	25.410946002322753	24.869992612235652
96-97	24.37772281296937	25.292415328842754	25.44390209713448	24.885959761053396
98-99	24.352855629215522	25.294004271178615	25.312865894886126	25.040274204719736
100-101	24.26236410410749	25.488648029123052	25.41072819996949	24.83825966679997
102-103	24.354298497148612	25.245465898875292	25.57699655947081	24.82323904450529
104-105	24.25220903981296	25.43610081476943	25.355582363547637	24.956107781869978
106-107	24.291804045773908	25.33424478509081	25.45637863348199	24.917572535653292
108-109	24.272842594794774	25.340474759143078	25.41110396201226	24.975578684049886
110-111	24.181002008717154	25.621475855714536	25.43024062349591	24.7672815120724
112-113	24.176574518044642	25.55709037820796	25.460002324135477	24.80633277961192
114-115	24.05671315979061	25.73888096997581	25.439654275027802	24.764751595205777
116-117	24.031809899161466	25.72462237081866	25.41158901082592	24.831978719193952
118-119	24.19278568386625	25.399691274727527	25.44121965335684	24.966303388049383
120-121	23.949322473395487	25.512252722633338	25.603496264289284	24.93492853968189
122-123	23.973777102796635	25.665384884001718	25.47794961849334	24.88288839470831
124-125	23.891138486463422	25.684094663798817	25.478487514647576	24.946279335090182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	203.0
2	276.5
3	279.0
4	232.0
5	218.5
6	240.5
7	291.0
8	399.0
9	493.5
10	512.5
11	544.0
12	652.0
13	767.0
14	869.5
15	960.0
16	1064.5
17	1182.0
18	1356.0
19	1508.0
20	1603.0
21	1800.0
22	2117.0
23	2514.0
24	3006.0
25	3770.5
26	5037.0
27	6445.0
28	7567.0
29	9128.5
30	11477.5
31	13913.0
32	16640.0
33	19325.0
34	22164.5
35	25156.0
36	28755.0
37	32485.0
38	34996.5
39	38177.5
40	41314.0
41	44070.5
42	47093.5
43	51651.5
44	56967.5
45	60715.5
46	64996.5
47	69833.5
48	74937.0
49	79595.5
50	80564.5
51	80592.0
52	80445.5
53	80698.5
54	81529.0
55	80402.0
56	76525.0
57	70093.5
58	65433.0
59	60797.0
60	54180.0
61	47956.0
62	41282.0
63	32831.5
64	25108.5
65	19698.5
66	15417.5
67	11946.0
68	9428.0
69	7628.5
70	6399.5
71	5237.0
72	4122.0
73	3553.5
74	2916.0
75	2276.5
76	1653.5
77	989.0
78	580.5
79	344.0
80	208.5
81	112.5
82	65.0
83	43.0
84	29.5
85	23.0
86	19.5
87	14.0
88	11.5
89	13.0
90	12.0
91	8.5
92	7.5
93	6.0
94	6.5
95	9.5
96	10.5
97	9.5
98	7.0
99	6.5
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03545185658487319
2	0.02499149773788299
3	0.025661372934980883
4	0.030453557037296592
5	0.03354528871620995
6	0.045499984541341604
7	0.04003792524192801
8	0.034988096833036185
9	0.029629095256253027
10-11	0.035786794183422135
12-13	0.03506539012500902
14-15	0.03483351024909052
16-17	0.03630208279657436
18-19	0.05006028876773881
20-21	0.06010841672420723
22-23	0.05302319829336411
24-25	0.06291673966588686
26-27	0.04740655241000484
28-29	0.0482052497603908
30-31	0.048153720899075575
32-33	0.056449867570826415
34-35	0.039522636628775774
36-37	0.05127121700864654
38-39	0.060082652293549615
40-41	0.04861748065091258
42-43	0.057815382395679814
44-45	0.05786691125699504
46-47	0.06327744169509342
48-49	0.048694773942885405
50-51	0.05091051497943998
52-53	0.06003112343223439
54-55	0.0466336194902765
56-57	0.043748003256624035
58-59	0.04516504694279266
60-61	0.03395751960673173
62-63	0.04292354147558047
64-65	0.04220213741716736
66-67	0.03493656797172096
68-69	0.030608143621242256
70-71	0.04918429812538003
72-73	0.032772355796481606
74-75	0.03959992992074861
76-77	0.03269506250450877
78-79	0.032798120227139224
80-81	0.024862675584594932
82-83	0.01803510146032793
84-85	0.018086630321643152
86-87	0.016978759803365866
88-89	0.02347139632908392
90-91	0.011593993795925097
92-93	0.018885327672029103
94-95	0.01491760535075696
96-97	0.011671287087897931
98-99	0.010924118598827203
100-101	0.010511887708305422
102-103	0.005951583481908217
104-105	0.005719703605989715
106-107	0.008553790978326961
108-109	0.01259880659157194
110-111	0.006080405635196274
112-113	0.007909680211886677
114-115	0.00814156008780518
116-117	0.00680180969360939
118-119	0.23881050776539942
120-121	0.351658714045737
122-123	0.02697535889851906
124-125	0.003400904846804695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1940660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.998618649517816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69188067021157	45.4232711578427
2	10.4003333820533	11.856092725430118
3	3.573664418127931	6.110818060506748
4	1.71290451562066	3.905327650755961
5	1.0320028123604224	2.94113673734808
6	0.7052634141678827	2.4119424234967255
7	0.5032773300436791	2.008027882707503
8	0.35849535576516794	1.634699205670562
9	0.284859930016744	1.461296026759745
>10	1.6342852636073908	16.604119557825616
>50	0.07882870658741649	3.054658270503672
>100	0.02356751453655405	2.201496765337959
>500	3.638210864047309E-4	0.1301783967363956
>1k	2.728658148035459E-4	0.25693513907828436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2280	0.11748580379870766	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.1528861315222655E-5
5	0.0	0.0	0.0	0.0	5.1528861315222655E-5
6	0.0	0.0	0.0	0.0	1.0305772263044531E-4
7	0.0	0.0	0.0	0.0	1.0305772263044531E-4
8	0.0	0.0	0.0	0.0	1.0305772263044531E-4
9	0.0	0.0	0.0	0.0	1.0305772263044531E-4
10-11	0.0	0.0	0.0	0.0	1.0305772263044531E-4
12-13	0.0	0.0	0.0	0.0	3.3493759854894727E-4
14-15	0.0	5.1528861315222655E-5	0.0	0.0	4.895241824946152E-4
16-17	0.0	5.1528861315222655E-5	0.0	0.0	5.668174744674492E-4
18-19	0.0	5.1528861315222655E-5	0.0	0.0	6.183463357826718E-4
20-21	0.0	5.1528861315222655E-5	0.0	0.0	6.698751970978945E-4
22-23	0.0	5.1528861315222655E-5	0.0	0.0	6.698751970978945E-4
24-25	0.0	5.1528861315222655E-5	0.0	5.1528861315222655E-5	7.729329197283398E-4
26-27	0.0	5.1528861315222655E-5	0.0	2.0611544526089065E-4	8.244617810435625E-4
28-29	0.0	5.1528861315222655E-5	0.0	0.0010821060876196758	8.502262117011739E-4
30-31	0.0	5.1528861315222655E-5	0.0	0.0045087753650819825	8.759906423587852E-4
32-33	0.0	5.1528861315222655E-5	0.0	0.010924118598827203	8.759906423587852E-4
34-35	0.0	5.1528861315222655E-5	0.0	0.01896262096400194	8.759906423587852E-4
36-37	0.0	1.0305772263044531E-4	0.0	0.02968062411756825	8.759906423587852E-4
38-39	0.0	1.0305772263044531E-4	0.0	0.04941617800129853	9.532839343316191E-4
40-41	0.0	1.0305772263044531E-4	0.0	0.07484567106036091	9.790483649892304E-4
42-43	0.0	1.0305772263044531E-4	0.0	0.09813671637484155	0.0010048127956468417
44-45	0.0	1.0305772263044531E-4	0.0	0.12338585841930064	0.0010563416569620645
46-47	0.0	1.0305772263044531E-4	0.0	0.15378788659528203	0.0010821060876196758
48-49	0.0	1.0305772263044531E-4	0.0	0.18887904115094867	0.0011336349489348984
50-51	0.0	1.0305772263044531E-4	0.0	0.22569641256067524	0.001185163810250121
52-53	0.0	1.0305772263044531E-4	0.0	0.26359589005802153	0.001417043686168623
54-55	0.0	1.0305772263044531E-4	0.0	0.3027835890882483	0.001571630270114291
56-57	0.0	1.0305772263044531E-4	0.0	0.3485154535055084	0.0015973947007719023
58-59	0.0	1.0305772263044531E-4	0.0	0.3984726845506168	0.001648923562087125
60-61	0.0	1.0305772263044531E-4	0.0	0.4557470139024868	0.001726216854059959
62-63	0.0	1.0305772263044531E-4	0.0	0.5134335741448786	0.0019065678686632382
64-65	0.0	1.0305772263044531E-4	0.0	0.572794822380015	0.0019065678686632382
66-67	0.0	1.0305772263044531E-4	0.0	0.6391897601846794	0.0019065678686632382
68-69	0.0	1.0305772263044531E-4	0.0	0.7157358836684427	0.0019323322993208495
70-71	0.0	1.0305772263044531E-4	0.0	0.8002432162254078	0.0020353900219512947
72-73	0.0	1.0305772263044531E-4	0.0	0.8909597765708573	0.002499149773788299
74-75	0.0	1.0305772263044531E-4	0.0	0.9867519297558562	0.0025249142044459104
76-77	0.0	1.0305772263044531E-4	0.0	1.0930302062185029	0.0026279719270763556
78-79	0.0	1.0305772263044531E-4	0.0	1.2136850349880968	0.002653736357733967
80-81	0.0	1.0305772263044531E-4	0.0	1.3427133037214143	0.0028083229416796347
82-83	0.0	1.0305772263044531E-4	0.0	1.4851648408273475	0.002937145094967691
84-85	0.0	1.0305772263044531E-4	0.0	1.6321251532983625	0.002937145094967691
86-87	0.0	1.0305772263044531E-4	0.0	1.7876392567477044	0.0029629095256253025
88-89	0.0	1.0305772263044531E-4	0.0	1.9587408407449014	0.003040202817598137
90-91	0.0	1.0305772263044531E-4	0.0	2.154189811713541	0.003040202817598137
92-93	0.0	1.0305772263044531E-4	0.0	2.3651489699380623	0.0031947894015438047
94-95	0.0	1.0305772263044531E-4	0.0	2.5829614667175083	0.0032720826935166386
96-97	0.0	1.0305772263044531E-4	0.0	2.8072408355920153	0.003323611554831861
98-99	0.0	1.0305772263044531E-4	0.0	3.059397318438057	0.0033493759854894725
100-101	0.0	1.0305772263044531E-4	0.0	3.34120866097101	0.0033493759854894725
102-103	0.0	1.0305772263044531E-4	0.0	3.650149948986427	0.003607020292065586
104-105	0.0	1.0305772263044531E-4	0.0	3.969087836096998	0.0038131357373264764
106-107	0.0	1.0305772263044531E-4	0.0	4.304746838704359	0.0038389001679840877
108-109	0.0	1.0305772263044531E-4	0.0	4.646022487195078	0.003941957890614533
110-111	0.0	1.0305772263044531E-4	0.0	5.0216936506137095	0.004096544474560201
112-113	0.0	1.0305772263044531E-4	0.0	5.429827996660929	0.004122308905217813
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2175	0.0	69.49941	1
GTATCAA	4590	0.0	61.716454	1
TCAACGC	5855	0.0	46.846794	4
CAACGCA	6015	0.0	45.501743	5
AACGCAG	6300	0.0	43.53441	6
ATCAACG	6420	0.0	43.184025	3
TATCAAC	6610	0.0	42.12274	2
GTACATG	10515	0.0	39.108887	1
TACATGG	10985	0.0	36.936573	2
ACGCAGA	7475	0.0	36.45244	7
ACATGGG	11325	0.0	35.775127	3
CGCAGAG	8000	0.0	33.98237	8
CATGGGG	9360	0.0	32.86401	4
GCAGAGT	8615	0.0	30.865955	9
ATGGGGG	5120	0.0	29.865448	5
AGAGTAC	7755	0.0	27.1933	10-11
CAGAGTA	8180	0.0	25.67136	10-11
GTGGTAT	1015	0.0	24.625774	1
GTATCGC	180	2.179784E-5	23.136507	6
CATGGGT	1865	0.0	22.969921	4
>>END_MODULE