FastQCFastQC Report
Mon 15 Aug 2016
SRR2921527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940660
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40670.20956787896901055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34840.17952655282223576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20290.10455205960858677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.078.670111
GTATCAA62700.062.483931
TCAACGC86800.045.2096254
CAACGCA88250.044.0987135
ATCAACG91700.042.599033
TATCAAC91850.042.529462
AACGCAG92700.041.844776
ACGCAGA107350.036.186867
GTACATG105100.035.4040071
CGCAGAG112700.034.5209248
TACATGG109850.033.2838442
ACATGGG112450.032.302463
GCAGAGT120800.031.5660089
CATGGGG93400.030.2201564
ATGGGGG53350.028.2194775
AGAGTAC112300.027.67838310-11
CAGAGTA117000.026.59193610-11
GAGTACT67200.025.4518612-13
AGTACTT70750.024.88950212-13
GTGGTAT12950.023.9444071
Produced by FastQC (version 0.11.5)