##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1940660
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250768810610822	31.0	31.0	34.0	28.0	34.0
2	31.314067379139054	33.0	31.0	34.0	28.0	34.0
3	31.404842682386406	33.0	31.0	34.0	28.0	34.0
4	34.91148372203271	37.0	35.0	37.0	32.0	37.0
5	34.56360568054167	37.0	35.0	37.0	31.0	37.0
6	34.53205971164449	37.0	35.0	37.0	31.0	37.0
7	34.376635268413835	37.0	35.0	37.0	30.0	37.0
8	34.34107623179743	37.0	35.0	37.0	30.0	37.0
9	35.90617934104892	39.0	37.0	39.0	30.0	39.0
10-11	35.781662939412364	39.0	37.0	39.0	30.0	39.0
12-13	35.67809224696752	39.0	35.0	39.0	30.0	39.0
14-15	36.85640735626024	40.0	37.0	41.0	30.0	41.0
16-17	36.84407289272722	40.0	37.0	41.0	30.0	41.0
18-19	36.875977760143456	40.0	37.0	41.0	30.0	41.0
20-21	36.86406402976307	40.0	37.0	41.0	30.0	41.0
22-23	36.80599538301402	40.0	37.0	41.0	30.0	41.0
24-25	36.737634103861566	40.0	37.0	41.0	30.0	41.0
26-27	36.61167489410819	40.0	36.0	41.0	30.0	41.0
28-29	36.42689110921027	40.0	36.0	41.0	29.0	41.0
30-31	36.36956009811095	40.0	36.0	41.0	29.5	41.0
32-33	36.2323225088372	40.0	35.0	41.0	29.0	41.0
34-35	36.09481104366556	40.0	35.0	41.0	28.5	41.0
36-37	35.95535487926788	40.0	35.0	41.0	28.0	41.0
38-39	35.777113456246845	40.0	35.0	41.0	27.0	41.0
40-41	35.5694444158173	39.5	35.0	41.0	26.5	41.0
42-43	35.39423443570744	39.0	35.0	41.0	26.0	41.0
44-45	35.15952742881288	39.0	35.0	41.0	24.5	41.0
46-47	34.852462564282256	39.0	34.5	41.0	23.5	41.0
48-49	34.56930296909299	39.0	34.0	41.0	23.0	41.0
50-51	34.34348675192976	39.0	34.0	41.0	22.0	41.0
52-53	34.137656518916245	38.0	34.0	41.0	21.5	41.0
54-55	33.91571656034545	38.0	33.0	40.0	20.0	41.0
56-57	33.654836498923046	37.5	33.0	40.0	19.5	41.0
58-59	33.50095792153185	37.0	33.0	40.0	19.5	41.0
60-61	33.376499747508575	37.0	33.0	40.0	19.5	41.0
62-63	33.10443637731494	36.0	33.0	40.0	18.0	41.0
64-65	32.81079529644554	36.0	33.0	39.0	18.0	41.0
66-67	32.510558778972104	35.0	33.0	39.0	16.5	41.0
68-69	32.19008043655251	35.0	32.0	39.0	14.0	41.0
70-71	31.88789071759092	35.0	32.0	37.5	13.0	40.0
72-73	31.575760565993015	35.0	32.0	37.0	11.5	39.0
74-75	31.289326054022858	35.0	32.0	36.5	9.0	39.0
76-77	31.003816485113312	35.0	32.0	36.0	7.5	39.0
78-79	30.72699854688611	35.0	31.0	36.0	7.0	37.0
80-81	30.508591407047085	35.0	31.0	35.0	7.0	37.0
82-83	30.325578411468264	35.0	31.0	35.0	3.5	36.5
84-85	30.139453330310307	35.0	31.0	35.0	2.0	36.0
86-87	29.997873661537827	35.0	31.0	35.0	2.0	36.0
88-89	29.897352962394237	35.0	31.0	35.0	2.0	36.0
90-91	29.80367143136871	35.0	31.0	35.0	2.0	35.0
92-93	29.862913132645595	35.0	31.0	35.0	2.0	35.0
94-95	29.830977347912565	35.0	31.0	35.0	2.0	35.0
96-97	29.81472875207404	35.0	31.0	35.0	2.0	35.0
98-99	29.776487895870474	35.0	31.0	35.0	2.0	35.0
100-101	29.726662578710336	35.0	31.0	35.0	2.0	35.0
102-103	28.847122113095544	33.5	29.5	35.0	2.0	35.0
104-105	29.549463842198016	34.5	31.0	35.0	2.0	35.0
106-107	29.654313738625003	35.0	31.0	35.0	2.0	35.0
108-109	29.658713530448402	35.0	31.0	35.0	2.0	35.0
110-111	29.62880463347521	35.0	31.0	35.0	2.0	35.0
112-113	29.58555594488473	35.0	31.0	35.0	2.0	35.0
114-115	29.517708666123895	35.0	31.0	35.0	2.0	35.0
116-117	29.446095142889533	35.0	31.0	35.0	2.0	35.0
118-119	29.354609514288953	35.0	31.0	35.0	2.0	35.0
120-121	29.231722197602878	35.0	31.0	35.0	2.0	35.0
122-123	29.109894314305443	35.0	31.0	35.0	2.0	35.0
124-125	28.206202271392208	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32194.0
3	12179.0
4	6745.0
5	5562.0
6	5988.0
7	7134.0
8	8217.0
9	9368.0
10	10743.0
11	11861.0
12	12592.0
13	14624.0
14	18816.0
15	15963.0
16	8721.0
17	7416.0
18	6526.0
19	5655.0
20	5518.0
21	5790.0
22	6892.0
23	9141.0
24	13007.0
25	18272.0
26	22682.0
27	24346.0
28	29080.0
29	34268.0
30	39578.0
31	48087.0
32	59704.0
33	76725.0
34	107209.0
35	171093.0
36	339985.0
37	551895.0
38	176698.0
39	386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.82180979218107	17.622459013517876	12.728380527074448	24.8273506672266
2	16.893465633875973	19.9648361976923	37.37895695835171	25.762741210080016
3	18.867260358905664	24.667793991301288	29.36957264569623	27.095373004096817
4	13.414322277012298	15.71942394113195	35.49922558318433	35.36702819867143
5	14.303621184273323	37.26142849632563	33.159312934685914	15.275637384715138
6	33.13514836814369	35.17568385924675	16.882709925139476	14.806457847470075
7	29.134589517278624	31.92286641647435	20.565725988907293	18.376818077339728
8	28.110566307061642	33.150567749947825	19.248771614306218	19.49009432868432
9	28.099675662973944	14.061870353823148	18.868128708310017	38.97032527489289
10-11	26.437677803821817	24.85075494075933	26.655377576021642	22.05618967939721
12-13	26.80126292855343	22.669304724542133	27.119520099517203	23.409912247387236
14-15	24.34915153883989	23.432657348765318	24.944173237009245	27.274017875385542
16-17	23.99870751096915	26.444567784040956	25.325673222904882	24.231051482085007
18-19	24.007248447414618	25.231795979858322	27.603979998490118	23.156975574236938
20-21	25.172193078881822	24.165343492016376	27.40996872308879	23.25249470601301
22-23	24.961903696874533	23.76459499391129	27.276515001208466	23.996986308005713
24-25	24.325167704731445	24.27692996689582	27.327322684440418	24.070579643932312
26-27	23.973803368602127	24.360898613409013	27.48587526220981	24.17942275577905
28-29	24.170835081267334	24.502444359008983	27.4064799478042	23.920240611919482
30-31	24.512667400590438	24.369438878168655	27.220557493444165	23.897336227796746
32-33	24.209142451631987	24.44489165817834	27.16952272187907	24.17644316831061
34-35	24.317788535255076	24.527606587286964	27.316367328479153	23.838237548978807
36-37	24.513350067138788	24.34415670922784	27.117242545094822	24.025250678538544
38-39	24.53687740722473	24.616609277243743	26.851910549809745	23.99460276572179
40-41	24.49791518398482	24.50412505494834	26.938394472647964	24.059565288418874
42-43	24.41799793195338	24.54005545539412	26.74319761852553	24.298748994126964
44-45	24.337276313361773	24.60148449281203	26.615836720221242	24.44540247360495
46-47	24.532075278190863	24.720288793333367	26.282378509644783	24.465257418830987
48-49	24.732095827023993	25.05862658652563	25.751348024941166	24.45792956150921
50-51	24.700486123011153	25.23817193010033	25.62676111685892	24.434580830029596
52-53	24.823849383814295	25.0749261819267	25.690802646884954	24.410421787374055
54-55	24.57730063025459	25.023745997453005	25.906793030246966	24.492160342045437
56-57	24.583483656829497	25.101558554008225	25.61875727995217	24.696200509210104
58-59	24.580889266946294	24.965978698967724	25.85835336645316	24.594778667632827
60-61	24.753198835320635	25.070937054589898	25.957743651484723	24.218120458604737
62-63	24.552729416791372	25.163761915082176	25.88328611839615	24.400222549730305
64-65	24.521477497414732	24.832559964933644	26.007571425663222	24.6383911119884
66-67	24.517719789316207	25.039868463060124	25.884881471684178	24.557530275939495
68-69	24.563679901701057	25.070868836472865	25.745617471152514	24.61983379067356
70-71	24.458654759686855	24.87978480442822	25.952052039438776	24.70950839644615
72-73	24.690648898385646	25.253238977349774	25.568549173676498	24.48756295058809
74-75	24.469904840046343	25.335770742024994	25.568619892058596	24.625704525870066
76-77	24.518186800741802	25.006255139070234	25.5609075324243	24.91465052776367
78-79	24.443979979439263	25.11113442026735	25.623915812825643	24.820969787467742
80-81	24.522306288847886	25.268211476812386	25.421567212641605	24.787915021698122
82-83	24.60012981367692	25.192304867858283	25.460950639109477	24.74661467935532
84-85	24.368517328909533	25.247814447796824	25.349751483382278	25.033916739911366
86-87	24.192372636563757	25.3287236819423	25.524351281409736	24.95455240008421
88-89	24.27344462892636	25.112194523592997	25.50433097274939	25.110029874731254
90-91	24.293485416972118	25.0577258005791	25.56758872483249	25.081200057616286
92-93	24.384338376327097	25.22573915983085	25.439110462485715	24.950812001356343
94-95	24.284261060998336	25.002473573984595	25.51308623703435	25.200179127982718
96-97	24.161077802655342	25.01784228195701	25.558804811876644	25.262275103511
98-99	24.11927812909662	25.049597449067928	25.394744164763498	25.43638025707195
100-101	24.020970479921587	25.181994617721116	25.52766328496291	25.269371617394388
102-103	24.12697103988457	24.91149644439864	25.677548180974956	25.28398433474183
104-105	24.060995002622874	25.088708854601986	25.467351122168296	25.38294502060684
106-107	24.144270877616062	25.004186730768733	25.45676588574476	25.394776505870443
108-109	24.09181008180229	24.938282358082926	25.465807137909422	25.50410042220536
110-111	24.00511375318257	25.157846679126415	25.448161929333953	25.388877638357066
112-113	23.94638019414814	25.10885210182857	25.534984439392993	25.409783264630292
114-115	23.736646588777027	25.27348373629103	25.537189614188122	25.452680060743816
116-117	23.714716676990435	25.1980411294786	25.478071183495437	25.609171010035524
118-119	23.68515237068738	24.916393173549967	25.598646521494793	25.79980793426786
120-121	23.532077731279724	24.972031600812745	25.7460258077083	25.749864860199228
122-123	23.54459620940851	25.04924903910912	25.619316650580963	25.7868381009014
124-125	23.409433601959403	25.04343021516286	25.535884142797983	26.011252040079757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	66.0
2	113.5
3	164.5
4	123.5
5	104.5
6	204.5
7	439.0
8	744.5
9	866.5
10	747.5
11	660.0
12	717.0
13	848.0
14	1021.0
15	1227.5
16	1400.0
17	1591.0
18	1871.0
19	2121.5
20	2374.5
21	2633.5
22	2980.0
23	3353.5
24	3754.5
25	4495.5
26	5695.5
27	7069.0
28	8059.5
29	9472.0
30	11892.5
31	14082.0
32	16543.5
33	19280.5
34	22049.5
35	24931.0
36	28368.0
37	31914.0
38	34254.5
39	37267.5
40	40422.0
41	42940.5
42	45910.0
43	50713.0
44	55994.5
45	60074.5
46	64714.5
47	69444.0
48	74448.5
49	79627.5
50	81073.0
51	81120.5
52	80559.5
53	80545.0
54	81777.5
55	81000.0
56	77248.0
57	70775.0
58	65919.5
59	60853.0
60	54147.0
61	47789.0
62	41119.0
63	32683.0
64	24652.5
65	19505.0
66	15174.0
67	11428.0
68	8994.0
69	7267.5
70	6152.5
71	5082.5
72	4008.0
73	3511.0
74	2873.0
75	2147.0
76	1518.5
77	869.0
78	468.5
79	264.5
80	150.5
81	69.0
82	38.0
83	20.5
84	11.0
85	7.5
86	6.5
87	5.0
88	3.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12047447775499058
2	0.0014428081168262345
3	0.01922026527057805
4	0.05853678645409294
5	0.024012449372893756
6	0.019838611606360725
7	0.014737254336153681
8	0.005410530438098379
9	0.0044314820731091486
10-11	0.007394391598734451
12-13	0.004689126379685262
14-15	0.0030144383869405256
16-17	0.011233291766718539
18-19	0.005487823730071213
20-21	0.012933744190120887
22-23	0.009429781620685748
24-25	0.013938556985767727
26-27	0.009352488328712912
28-29	0.014118908000371007
30-31	0.020817659971349954
32-33	0.012907979759463274
34-35	0.020998010985953233
36-37	0.014247730153659066
38-39	0.020972246555295622
40-41	0.010357301124359754
42-43	0.009352488328712912
44-45	0.017210639679284368
46-47	0.015999711438376633
48-49	0.02133294858450218
50-51	0.015845124854430966
52-53	0.021487535168447848
54-55	0.017159110817969142
56-57	0.02061154452608906
58-59	0.01708181752599631
60-61	0.019761318314387887
62-63	0.021616357321735905
64-65	0.01744251955520287
66-67	0.011155998474745705
68-69	0.02349716075974153
70-71	0.014711489905496068
72-73	0.017957808168355096
74-75	0.017932043737697485
76-77	0.013165624066039388
78-79	0.02857275359929096
80-81	0.02061154452608906
82-83	0.009610132635289025
84-85	0.012701864314202387
86-87	0.012289633423680604
88-89	0.02017354920490967
90-91	0.012186575701050159
92-93	0.007523213752022508
94-95	0.007626271474652954
96-97	0.0023187987591850195
98-99	0.0018550390073480156
100-101	0.011233291766718539
102-103	0.00309173167891336
104-105	0.0021642121752393517
106-107	2.5764430657611325E-4
108-109	0.01916873640926283
110-111	5.668174744674492E-4
112-113	0.009429781620685748
114-115	0.0026279719270763556
116-117	0.007935444642544288
118-119	0.009197901744767244
120-121	0.003967722321272144
122-123	6.698751970978945E-4
124-125	0.024656560139334042
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1940660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.563666724842484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16370479172394	43.98625709751166
2	10.560685700842443	11.735808413348382
3	3.661148350174041	6.102804802778321
4	1.7863874373313868	3.9703294483730645
5	1.0859893577474338	3.0170775370302083
6	0.7211127016305703	2.4040599494671073
7	0.5353728720782347	2.082309588637373
8	0.39138362143264543	1.7397367282276346
9	0.2929477952986477	1.4649528299176298
>10	1.6865692255702975	16.90762066682184
>50	0.08597314376959461	3.2407003604330566
>100	0.027888677699868816	2.6582223307161432
>500	5.575498005543668E-4	0.19548071630618793
>1k	2.787749002771825E-4	0.49463953043139863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4067	0.20956787896901055	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3484	0.17952655282223576	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2029	0.10455205960858677	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0305772263044531E-4
2	0.0	0.0	0.0	0.0	1.0305772263044531E-4
3	0.0	0.0	0.0	0.0	1.0305772263044531E-4
4	0.0	0.0	0.0	0.0	1.0305772263044531E-4
5	0.0	0.0	0.0	0.0	1.0305772263044531E-4
6	0.0	0.0	0.0	0.0	1.5458658394566796E-4
7	0.0	0.0	0.0	0.0	1.5458658394566796E-4
8	0.0	0.0	0.0	0.0	1.5458658394566796E-4
9	0.0	0.0	0.0	0.0	2.0611544526089062E-4
10-11	0.0	0.0	0.0	5.1528861315222655E-5	2.0611544526089062E-4
12-13	0.0	0.0	0.0	5.1528861315222655E-5	2.576443065761133E-4
14-15	0.0	0.0	0.0	5.1528861315222655E-5	3.607020292065586E-4
16-17	0.0	0.0	0.0	5.1528861315222655E-5	4.637597518370039E-4
18-19	0.0	0.0	0.0	5.1528861315222655E-5	5.410530438098379E-4
20-21	0.0	0.0	0.0	5.1528861315222655E-5	6.183463357826718E-4
22-23	0.0	0.0	0.0	5.1528861315222655E-5	6.956396277555058E-4
24-25	0.0	0.0	0.0	1.0305772263044531E-4	8.502262117011739E-4
26-27	0.0	0.0	0.0	2.5764430657611325E-4	9.790483649892304E-4
28-29	0.0	0.0	0.0	0.0013655148248534004	9.790483649892304E-4
30-31	0.0	0.0	0.0	0.005178650562179877	9.790483649892304E-4
32-33	0.0	0.0	0.0	0.011825873671843599	0.0010305772263044532
34-35	0.0	0.0	0.0	0.019787082745045502	0.0011078705182772871
36-37	0.0	0.0	0.0	0.030917316789133592	0.0011336349489348984
38-39	0.0	0.0	0.0	0.051296981439304154	0.001185163810250121
40-41	0.0	0.0	0.0	0.07719023425020355	0.0012366926715653439
42-43	0.0	0.0	0.0	0.10104809703915163	0.0013139859635381778
44-45	0.0	0.0	0.0	0.1273278163099152	0.001339750394195789
46-47	0.0	0.0	0.0	0.15837395525233683	0.001339750394195789
48-49	0.0	0.0	0.0	0.19387734069852525	0.0013912792555110117
50-51	0.0	0.0	0.0	0.2318025826265291	0.0014428081168262343
52-53	0.0	0.0	0.0	0.2709645172260983	0.0016489235620871252
54-55	2.5764430657611327E-5	0.0	0.0	0.3108993847453959	0.0017519812847175704
56-57	5.1528861315222655E-5	0.0	0.0	0.3575845330969876	0.0017777457153751817
58-59	1.0305772263044531E-4	0.0	0.0	0.4087784568136613	0.001803510146032793
60-61	1.0305772263044531E-4	0.0	0.0	0.46682571908525966	0.001803510146032793
62-63	1.0305772263044531E-4	0.0	0.0	0.5261096740284233	0.001958096729978461
64-65	1.0305772263044531E-4	0.0	0.0	0.5869394948110437	0.001958096729978461
66-67	1.0305772263044531E-4	0.0	0.0	0.6543907742726701	0.001958096729978461
68-69	1.0305772263044531E-4	0.0	0.0	0.7322251192893139	0.001958096729978461
70-71	1.0305772263044531E-4	0.0	0.0	0.8191543083280945	0.0020096255912936834
72-73	1.0305772263044531E-4	0.0	0.0	0.9115197922356312	0.0021384477445817404
74-75	1.0305772263044531E-4	0.0	0.0	1.009295806581266	0.0021642121752393517
76-77	1.0305772263044531E-4	0.0	0.0	1.1169138334381086	0.0021642121752393517
78-79	1.0305772263044531E-4	0.0	0.0	1.2400162831201755	0.0021642121752393517
80-81	1.0305772263044531E-4	0.0	0.0	1.3717755815031998	0.0021642121752393517
82-83	1.0305772263044531E-4	0.0	0.0	1.5160821576164811	0.002370327620500242
84-85	1.0305772263044531E-4	0.0	0.0	1.6658507930291755	0.002370327620500242
86-87	1.0305772263044531E-4	0.0	0.0	1.8245339214494039	0.0024733853431306877
88-89	1.0305772263044531E-4	0.0	0.0	2.00006698751971	0.0025249142044459104
90-91	1.0305772263044531E-4	0.0	0.0	2.200436964743953	0.0025249142044459104
92-93	1.0305772263044531E-4	0.0	0.0	2.415750311749611	0.002576443065761133
94-95	1.0305772263044531E-4	0.0	0.0	2.638767223521895	0.002679500788391578
96-97	1.0305772263044531E-4	0.0	0.0	2.868740531571733	0.0027825585110220234
98-99	1.0305772263044531E-4	0.0	0.0	3.12602413611864	0.002834087372337246
100-101	1.0305772263044531E-4	0.0	0.0	3.4146115239145445	0.002834087372337246
102-103	1.0305772263044531E-4	0.0	0.0	3.7311275545432996	0.0031174961095709708
104-105	1.0305772263044531E-4	0.0	0.0	4.057743241989838	0.003143260540228582
106-107	1.0305772263044531E-4	0.0	0.0	4.399869116692259	0.0031947894015438047
108-109	1.0305772263044531E-4	0.0	0.0	4.749801613883936	0.00329784712417425
110-111	1.0305772263044531E-4	0.0	0.0	5.135675491842981	0.003426669277462307
112-113	1.0305772263044531E-4	0.0	0.0	5.555171951810209	0.0035039625694351408
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3085	0.0	78.67011	1
GTATCAA	6270	0.0	62.48393	1
TCAACGC	8680	0.0	45.209625	4
CAACGCA	8825	0.0	44.098713	5
ATCAACG	9170	0.0	42.59903	3
TATCAAC	9185	0.0	42.52946	2
AACGCAG	9270	0.0	41.84477	6
ACGCAGA	10735	0.0	36.18686	7
GTACATG	10510	0.0	35.404007	1
CGCAGAG	11270	0.0	34.520924	8
TACATGG	10985	0.0	33.283844	2
ACATGGG	11245	0.0	32.30246	3
GCAGAGT	12080	0.0	31.566008	9
CATGGGG	9340	0.0	30.220156	4
ATGGGGG	5335	0.0	28.219477	5
AGAGTAC	11230	0.0	27.678383	10-11
CAGAGTA	11700	0.0	26.591936	10-11
GAGTACT	6720	0.0	25.45186	12-13
AGTACTT	7075	0.0	24.889502	12-13
GTGGTAT	1295	0.0	23.944407	1
>>END_MODULE