FastQCFastQC Report
Mon 15 Aug 2016
SRR2921526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939277
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.068.098281
GTATCAA44300.058.710691
TCAACGC54400.046.5912634
CAACGCA54600.046.529575
AACGCAG57000.044.3570946
ATCAACG57550.044.1410563
TATCAAC59700.043.3486062
GTACATG96300.038.2563821
ACGCAGA66100.037.5304347
TACATGG102250.035.491692
ACATGGG103750.035.265273
CGCAGAG71550.034.6699338
CATGGGG83450.033.7944834
ATGGGGG45000.032.392725
GCAGAGT80550.030.3530889
AGAGTAC71100.026.22977410-11
CAGAGTA76200.025.5281510-11
GAGTACT27950.023.62477912-13
TATAGGG3256.1118044E-1021.966362
ATGGGGA33950.021.7307995
Produced by FastQC (version 0.11.5)