##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921526_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1939277
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.913541489947026	31.0	30.0	34.0	26.0	34.0
2	29.937577767384443	31.0	30.0	34.0	25.0	34.0
3	30.089022867800733	31.0	30.0	34.0	26.0	34.0
4	33.473625995667454	37.0	35.0	37.0	27.0	37.0
5	33.50713384421101	37.0	35.0	37.0	27.0	37.0
6	33.51069651215376	37.0	35.0	37.0	27.0	37.0
7	33.38236208648893	37.0	35.0	37.0	27.0	37.0
8	33.301779993265534	37.0	35.0	37.0	26.0	37.0
9	34.77206505311	39.0	35.0	39.0	27.0	39.0
10-11	34.661941538006175	39.0	35.0	39.0	26.5	39.0
12-13	34.519081338045055	39.0	35.0	39.0	25.5	39.0
14-15	35.67266305948041	40.0	36.0	41.0	25.0	41.0
16-17	35.59830596660508	40.0	36.0	41.0	25.0	41.0
18-19	35.61450478709334	40.0	36.0	41.0	25.0	41.0
20-21	35.642043400710676	40.0	36.0	41.0	25.0	41.0
22-23	35.61155549207256	40.0	36.0	41.0	25.0	41.0
24-25	35.57695238998864	40.0	35.5	41.0	25.0	41.0
26-27	35.5019331946906	40.0	35.0	41.0	25.0	41.0
28-29	35.46518651023035	40.0	35.0	41.0	25.0	41.0
30-31	35.3706360669466	40.0	35.0	41.0	25.0	41.0
32-33	35.27098062834757	40.0	35.0	41.0	25.0	41.0
34-35	35.11333012251473	40.0	35.0	41.0	24.0	41.0
36-37	34.97974502868853	40.0	35.0	41.0	24.0	41.0
38-39	34.86112530597743	39.0	34.5	41.0	23.5	41.0
40-41	34.72182055477376	39.0	34.0	41.0	23.0	41.0
42-43	34.529726284589565	39.0	34.0	41.0	21.5	41.0
44-45	34.33608788223653	39.0	34.0	41.0	20.5	41.0
46-47	34.09084777471192	39.0	33.0	41.0	18.0	41.0
48-49	33.94899233064693	39.0	33.0	41.0	17.5	41.0
50-51	33.7657126857071	38.0	33.0	40.5	15.0	41.0
52-53	33.58368402244754	38.0	33.0	40.0	14.5	41.0
54-55	33.387417320991275	38.0	33.0	40.0	13.5	41.0
56-57	33.15588902462103	37.5	33.0	40.0	12.5	41.0
58-59	32.917985156323724	37.0	32.0	40.0	10.0	41.0
60-61	32.61193656192488	36.5	32.0	40.0	8.0	41.0
62-63	32.297878281442	36.0	32.0	39.5	7.0	41.0
64-65	31.99614727550525	35.5	31.0	39.0	6.5	41.0
66-67	31.956842421170364	35.0	32.0	39.0	3.5	41.0
68-69	31.70981582311346	35.0	32.0	39.0	2.0	40.5
70-71	31.408514358701723	35.0	31.0	37.5	2.0	40.0
72-73	31.07709471107016	35.0	31.0	37.0	2.0	39.5
74-75	30.76848846245276	35.0	31.0	36.5	2.0	39.0
76-77	30.478713974331672	35.0	31.0	36.0	2.0	39.0
78-79	30.19829245641546	35.0	31.0	36.0	2.0	37.5
80-81	29.94934813335073	35.0	30.5	35.0	2.0	37.0
82-83	29.742146686625993	35.0	30.0	35.0	2.0	37.0
84-85	29.535408814728374	35.0	30.0	35.0	2.0	36.0
86-87	29.37655554105989	35.0	30.0	35.0	2.0	36.0
88-89	29.245346074851607	34.5	30.0	35.0	2.0	36.0
90-91	29.128870450172926	34.0	30.0	35.0	2.0	35.0
92-93	29.024715912167267	34.0	30.0	35.0	2.0	35.0
94-95	28.958410273519462	34.0	30.0	35.0	2.0	35.0
96-97	28.856664622949687	34.0	30.0	35.0	2.0	35.0
98-99	28.793181685751957	34.0	29.0	35.0	2.0	35.0
100-101	28.72776555386363	34.0	29.0	35.0	2.0	35.0
102-103	27.74747650799757	33.5	28.0	34.5	2.0	35.0
104-105	28.569164951680445	34.0	29.0	35.0	2.0	35.0
106-107	28.89043545610039	34.5	30.0	35.0	2.0	35.0
108-109	28.92881599688956	35.0	30.0	35.0	2.0	35.0
110-111	28.869945861266853	35.0	30.0	35.0	2.0	35.0
112-113	28.801216123328437	35.0	30.0	35.0	2.0	35.0
114-115	28.684079169711186	35.0	30.0	35.0	2.0	35.0
116-117	28.619138214912052	35.0	30.0	35.0	2.0	35.0
118-119	28.462084065350126	35.0	30.0	35.0	2.0	35.0
120-121	28.286865414275525	34.5	29.5	35.0	2.0	35.0
122-123	28.16263251717006	34.5	29.5	35.0	2.0	35.0
124-125	27.32323618544437	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72265.0
3	12890.0
4	8392.0
5	7126.0
6	7576.0
7	8655.0
8	9770.0
9	10030.0
10	10551.0
11	11265.0
12	11336.0
13	12226.0
14	13295.0
15	11708.0
16	8843.0
17	7567.0
18	7062.0
19	6486.0
20	6164.0
21	6464.0
22	7805.0
23	10146.0
24	14658.0
25	20696.0
26	24665.0
27	25919.0
28	30444.0
29	36173.0
30	41472.0
31	48921.0
32	61107.0
33	79631.0
34	111634.0
35	173128.0
36	334165.0
37	513498.0
38	155388.0
39	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66571820023852	17.242779206094394	12.23330829767127	24.85819429599581
2	16.558884331622295	19.693529552544277	38.39468450327628	25.35290161255715
3	18.82624243152591	24.674502101558748	29.04999435217369	27.449261114741653
4	13.240535658467692	15.429685283628498	35.9593397218133	35.370439336090506
5	14.088994689617287	37.49826555008498	33.65374447748568	14.758995282812052
6	33.08682456640854	35.020323431195685	17.506782645301314	14.386069357094474
7	29.38451189428494	31.443120277906456	21.049123701182467	18.123244126626133
8	27.62786355031234	33.74277180837619	19.57489721911286	19.05446742219861
9	27.75275839355781	14.640749897225952	18.957517824914287	38.64897388430195
10-11	26.027413512596166	25.108273677064858	27.25816901422981	21.606143796109162
12-13	26.331948741293644	22.88373424602064	27.315907505296998	23.468409507388717
14-15	23.946561148689252	23.96229393276788	24.52962844322296	27.561516475319902
16-17	23.955843061861344	26.951532444661325	25.093657205214832	23.9989672882625
18-19	24.039829192907078	26.24977622454828	26.520705742632693	23.189688839911945
20-21	25.18080787077287	25.179466355572732	26.17450945690821	23.465216316746186
22-23	25.07937629886144	24.816895362587424	25.965826658879514	24.137901679671618
24-25	24.406482926475633	25.248310861799094	26.13428123038454	24.210924981340728
26-27	24.05717869037212	25.367472087650057	26.222841192574386	24.35250802940344
28-29	24.153012371410586	25.396344294602596	26.265412672699316	24.185230661287495
30-31	24.53344050418451	25.288236623334008	26.073471190267128	24.104851682214353
32-33	24.18573466204135	25.375592020012654	26.08473905973846	24.353934258207534
34-35	24.44271403327634	25.380643886626142	26.151126797726825	24.02551528237069
36-37	24.522883262432973	25.251698216488972	25.996119762666297	24.229298758411762
38-39	24.484567266625714	25.522798177291268	25.881550762613674	24.11108379346934
40-41	24.473156900396113	25.322183231201162	26.041072300083883	24.16358756831884
42-43	24.2700217734529	25.351161422807433	25.96584870030029	24.412968103439383
44-45	24.287882024309628	25.385855258745142	25.913803902872928	24.412458814072302
46-47	24.425749220736133	25.39979680575342	25.81062275579488	24.363831217715568
48-49	24.42017427983364	25.568746535336405	25.62456808591153	24.38651109891842
50-51	24.414689060334748	25.67665452879503	25.51671941086094	24.391937000009285
52-53	24.494054759675045	25.578928902200857	25.67675641928585	24.250259918838246
54-55	24.3356258781475	25.47820104834722	25.804219957392355	24.38195311611293
56-57	24.35233962514306	25.607279538820155	25.55981819208897	24.480562643947813
58-59	24.415413636139693	25.477732800832438	25.643849142630742	24.463004420397127
60-61	24.480902617029194	25.589907715734235	25.745558911591925	24.183630755644643
62-63	24.299470890463517	25.66440480271434	25.722337821723645	24.313786485098497
64-65	24.31253382196889	25.365483613530127	25.83815288329544	24.483829681205535
66-67	24.339321265649556	25.620559441677692	25.64921423702287	24.390905055649885
68-69	24.28380946977219	25.58543246874831	25.664325744895404	24.466432316584093
70-71	24.272695661505157	25.39495501779374	25.90588663803021	24.426462682670888
72-73	24.416393517882103	25.66527386362669	25.56427608501042	24.35405653348079
74-75	24.22426204882206	25.794747406382946	25.610662627823753	24.37032791697124
76-77	24.251005588378813	25.43759471719213	25.733210362176877	24.57818933225218
78-79	24.145516275192712	25.51143678919829	25.751242097618142	24.591804837990853
80-81	24.31527931043998	25.766743011081843	25.455829284225334	24.462148394252843
82-83	24.421223262109653	25.687818360406993	25.55344070995547	24.337517667527877
84-85	24.221509858919575	25.705189384142123	25.471684246384406	24.601616510553892
86-87	24.114310970875312	25.84418474576402	25.627960194052836	24.41354408930783
88-89	24.236067772132966	25.60045903499497	25.61827887665369	24.545194316218378
90-91	24.20169506035442	25.56984207698012	25.705372642010555	24.5230902206549
92-93	24.264279628410925	25.751579748684318	25.608561691171765	24.37557893173299
94-95	24.253465493593506	25.49819895157811	25.65748130682186	24.590854248006522
96-97	24.251258603422084	25.561949280422308	25.682136753282787	24.504655362872825
98-99	24.136296769984696	25.587115522716342	25.593561887031335	24.683025820267627
100-101	24.07964180988736	25.627539217010938	25.706829314077485	24.585989659024218
102-103	24.16673735844133	25.453215925683647	25.759661545831865	24.620385170043154
104-105	23.998019773898402	25.6423551798512	25.65385493038912	24.705770115861274
106-107	24.00478362922026	25.607938885475246	25.69676813657375	24.690509348730746
108-109	24.00484776596636	25.59757095981898	25.687357774153412	24.710223500061243
110-111	23.973425716595983	25.76821605967095	25.687846540371957	24.57051168336111
112-113	23.93253565520172	25.747114166780122	25.77091352401698	24.54943665400118
114-115	23.84225100219507	25.910149379731045	25.743243643954717	24.504355974119168
116-117	23.796301262203816	25.860042657909553	25.723023719695455	24.620632360191177
118-119	23.97592718508451	25.572158358330576	25.703599008328627	24.748315448256292
120-121	23.764302136465318	25.682499944372317	25.86718385751861	24.686014061643753
122-123	23.765891062629628	25.832483557679264	25.750729723888675	24.650895655802433
124-125	23.669198359234258	25.801915361178086	25.808387047036778	24.72049923255088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	150.0
1	188.0
2	225.5
3	233.0
4	214.0
5	187.5
6	190.5
7	246.5
8	353.0
9	416.0
10	438.0
11	474.5
12	546.0
13	645.5
14	755.5
15	842.5
16	905.5
17	1008.0
18	1114.0
19	1230.5
20	1368.0
21	1544.5
22	1863.5
23	2289.0
24	2760.0
25	3684.0
26	5140.0
27	6668.0
28	7824.0
29	9558.0
30	12329.5
31	14756.0
32	17811.0
33	21159.0
34	24643.0
35	28402.5
36	32733.0
37	36937.0
38	39106.5
39	41886.0
40	44687.0
41	46745.5
42	49244.0
43	54011.0
44	59155.5
45	62796.0
46	66307.0
47	69669.0
48	74457.0
49	78180.0
50	78616.0
51	78608.5
52	78386.0
53	78420.0
54	79342.0
55	78335.5
56	74281.0
57	68422.5
58	63759.0
59	59156.0
60	52579.5
61	45822.5
62	39049.5
63	31130.0
64	23732.0
65	18327.0
66	14139.0
67	11005.0
68	8668.0
69	6845.5
70	5537.5
71	4446.5
72	3406.0
73	2712.5
74	2139.0
75	1584.5
76	1113.5
77	648.5
78	367.0
79	220.0
80	115.5
81	66.0
82	46.0
83	27.0
84	20.5
85	18.5
86	17.0
87	16.5
88	16.0
89	10.0
90	10.0
91	9.5
92	7.5
93	8.5
94	7.0
95	4.5
96	5.5
97	10.0
98	12.0
99	7.0
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03656001695477232
2	0.025421845357831808
3	0.02464836121915539
4	0.029856487752909975
5	0.03207380895044906
6	0.045068342480212985
7	0.04037587203890935
8	0.03542557355138023
9	0.028773609958762984
10-11	0.03460052380345871
12-13	0.03503883148204202
14-15	0.033517646009311716
16-17	0.03565761879298316
18-19	0.0489873287828402
20-21	0.06033176281676109
22-23	0.05367979922414384
24-25	0.06355461339457953
26-27	0.04808493062105104
28-29	0.04831697586265397
30-31	0.04842010708114416
32-33	0.056902649801962274
34-35	0.038803120956933945
36-37	0.05123043278500183
38-39	0.060460676839873835
40-41	0.04842010708114416
42-43	0.058630097711672956
44-45	0.05749565430828087
46-47	0.06342569937146679
48-49	0.04937407085217841
50-51	0.0511015187618891
52-53	0.060434894035251284
54-55	0.046022306251247244
56-57	0.044217509927668916
58-59	0.045197256503325726
60-61	0.03338873198619898
62-63	0.04287680408729645
64-65	0.04218066836248767
66-67	0.0352450939190224
68-69	0.030011184580645257
70-71	0.04924515682906568
72-73	0.031996460536581416
74-75	0.03960238790023292
76-77	0.031506587248753014
78-79	0.03202224334120397
80-81	0.024519447196042647
82-83	0.01745495872946464
84-85	0.017919049212670495
86-87	0.01652677776305293
88-89	0.022018515147655543
90-91	0.011911655735616934
92-93	0.018615184937479275
94-95	0.0137938004730629
96-97	0.011499130861656173
98-99	0.010287339044396443
100-101	0.010055293802793516
102-103	0.005543302993847707
104-105	0.005388606166112422
106-107	0.008430977111573024
108-109	0.01234996341420024
110-111	0.005620651407715349
112-113	0.007683275777519147
114-115	0.00817314906534755
116-117	0.006651963592617248
118-119	0.2353196577899908
120-121	0.3499242243372143
122-123	0.027329772899900323
124-125	0.003119719359328244
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1939277.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.22978613376999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57933148359861	45.54308121474736
2	10.455265303008803	11.967051945260392
3	3.613397046830723	6.20381820619555
4	1.7857298817222576	4.0878775689458875
5	1.0477595781828533	2.998152828950687
6	0.694288437668331	2.3840387281744726
7	0.5004294509385174	2.00476293165759
8	0.38334782637698844	1.755113129472051
9	0.2763518877089974	1.4234003488124585
>10	1.5577535491032495	16.017354718778616
>50	0.08021374827145629	3.0968692437584653
>100	0.025769029734946842	2.2928000889966373
>500	1.8138842718005966E-4	0.07339240372521598
>1k	1.8138842718005966E-4	0.1522866425245995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.156560924509495E-5
2	0.0	0.0	0.0	0.0	5.156560924509495E-5
3	0.0	0.0	0.0	0.0	5.156560924509495E-5
4	0.0	0.0	0.0	0.0	1.031312184901899E-4
5	0.0	0.0	0.0	0.0	1.031312184901899E-4
6	0.0	0.0	0.0	0.0	1.031312184901899E-4
7	0.0	0.0	0.0	0.0	1.031312184901899E-4
8	0.0	0.0	0.0	0.0	1.031312184901899E-4
9	0.0	0.0	0.0	0.0	1.5469682773528484E-4
10-11	0.0	0.0	0.0	0.0	1.5469682773528484E-4
12-13	0.0	0.0	0.0	0.0	2.5782804622547477E-4
14-15	0.0	0.0	0.0	0.0	3.3517646009311716E-4
16-17	0.0	0.0	0.0	0.0	3.6095926471566464E-4
18-19	0.0	0.0	0.0	0.0	3.6095926471566464E-4
20-21	0.0	0.0	0.0	0.0	3.6095926471566464E-4
22-23	0.0	0.0	0.0	7.734841386764242E-5	5.156560924509495E-4
24-25	0.0	0.0	0.0	1.5469682773528484E-4	5.414388970734971E-4
26-27	0.0	0.0	0.0	1.8047963235783232E-4	5.672217016960445E-4
28-29	0.0	0.0	0.0	8.250497479215192E-4	5.672217016960445E-4
30-31	0.0	0.0	0.0	0.0031712849685733395	5.672217016960445E-4
32-33	0.0	0.0	0.0	0.008430977111573024	5.672217016960445E-4
34-35	0.0	0.0	0.0	0.01693930263701369	5.672217016960445E-4
36-37	0.0	0.0	0.0	0.02900565520036591	6.187873109411394E-4
38-39	0.0	0.0	0.0	0.04767240574709028	6.703529201862343E-4
40-41	0.0	0.0	0.0	0.0684791290774861	6.703529201862343E-4
42-43	0.0	0.0	0.0	0.0887959791200535	6.961357248087819E-4
44-45	0.0	0.0	0.0	0.11249037656817464	7.219185294313293E-4
46-47	0.0	0.0	0.0	0.13896931691553088	7.219185294313293E-4
48-49	0.0	0.0	0.0	0.16743353321882332	7.734841386764242E-4
50-51	0.0	0.0	0.0	0.19986830143398804	7.734841386764242E-4
52-53	0.0	0.0	0.0	0.23343751305254484	8.250497479215192E-4
54-55	0.0	0.0	0.0	0.27242111364183663	9.023981617891617E-4
56-57	0.0	5.156560924509495E-5	0.0	0.31527213492451056	9.539637710342566E-4
58-59	0.0	5.156560924509495E-5	0.0	0.36348597956867434	9.79746575656804E-4
60-61	0.0	1.031312184901899E-4	0.0	0.4155414621015977	9.79746575656804E-4
62-63	0.0	1.031312184901899E-4	0.0	0.4680868179223494	0.001031312184901899
64-65	0.0	1.031312184901899E-4	0.0	0.5204001285014983	0.001031312184901899
66-67	0.0	1.031312184901899E-4	0.0	0.5806803257090143	0.001031312184901899
68-69	0.0	1.031312184901899E-4	0.0	0.6520213460996032	0.001031312184901899
70-71	0.0	1.031312184901899E-4	0.0	0.7342684928455295	0.001031312184901899
72-73	0.0	1.031312184901899E-4	0.0	0.8196869245600293	0.001134443403392089
74-75	0.0	1.031312184901899E-4	0.0	0.9068070213796173	0.001134443403392089
76-77	0.0	1.031312184901899E-4	0.0	1.0049621585776554	0.0011602262080146364
78-79	0.0	1.5469682773528484E-4	0.0	1.1166532682025312	0.0012117918172597313
80-81	0.0	2.062624369803798E-4	0.0	1.2370589657898279	0.0012633574265048264
82-83	0.0	2.062624369803798E-4	0.0	1.3646838486714379	0.0013149230357499212
84-85	0.0	2.062624369803798E-4	0.0	1.5028023330344247	0.0013407058403724686
86-87	0.0	2.062624369803798E-4	0.0	1.64556172222947	0.001366488644995016
88-89	0.0	2.062624369803798E-4	0.0	1.8044353643136075	0.0013922714496175637
90-91	0.0	2.062624369803798E-4	0.0	1.9911544353900963	0.0013922714496175637
92-93	0.0	2.062624369803798E-4	0.0	2.186768574061364	0.0014696198634852062
94-95	0.0	2.062624369803798E-4	0.0	2.3861212194029013	0.0015985338865979434
96-97	0.0	2.062624369803798E-4	0.0	2.5991645339990113	0.001727447909710681
98-99	0.0	2.062624369803798E-4	0.0	2.83092616475109	0.0017532307143332284
100-101	0.0	2.062624369803798E-4	0.0	3.0941943827519225	0.0018047963235783232
102-103	0.0	2.062624369803798E-4	0.0	3.381079649787008	0.0019337103466910608
104-105	0.0	2.062624369803798E-4	0.0	3.6860644456671223	0.002062624369803798
106-107	2.5782804622547475E-5	2.062624369803798E-4	0.0	4.000382616820598	0.002114189979048893
108-109	5.156560924509495E-5	2.062624369803798E-4	0.0	4.3350692036258875	0.002165755588293988
110-111	5.156560924509495E-5	2.062624369803798E-4	0.0	4.692908748982224	0.0023720180252743677
112-113	5.156560924509495E-5	2.062624369803798E-4	0.0	5.080295388435999	0.0024235836345194626
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2150	0.0	68.09828	1
GTATCAA	4430	0.0	58.71069	1
TCAACGC	5440	0.0	46.591263	4
CAACGCA	5460	0.0	46.52957	5
AACGCAG	5700	0.0	44.357094	6
ATCAACG	5755	0.0	44.141056	3
TATCAAC	5970	0.0	43.348606	2
GTACATG	9630	0.0	38.256382	1
ACGCAGA	6610	0.0	37.530434	7
TACATGG	10225	0.0	35.49169	2
ACATGGG	10375	0.0	35.26527	3
CGCAGAG	7155	0.0	34.669933	8
CATGGGG	8345	0.0	33.794483	4
ATGGGGG	4500	0.0	32.39272	5
GCAGAGT	8055	0.0	30.353088	9
AGAGTAC	7110	0.0	26.229774	10-11
CAGAGTA	7620	0.0	25.52815	10-11
GAGTACT	2795	0.0	23.624779	12-13
TATAGGG	325	6.1118044E-10	21.96636	2
ATGGGGA	3395	0.0	21.730799	5
>>END_MODULE