FastQCFastQC Report
Mon 15 Aug 2016
SRR2921526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939277
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.18661593985799863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31300.1614003569371472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.079.209971
GTATCAA59100.057.5913051
TCAACGC75800.044.379554
CAACGCA76850.043.6709255
AACGCAG79600.042.229336
ATCAACG80100.041.92283
TATCAAC81500.041.4959372
GTACATG93550.037.6575431
TACATGG98450.035.6821632
ACGCAGA94100.035.6570857
ACATGGG100600.033.9125373
CGCAGAG100600.033.4123348
CATGGGG83050.031.2572334
GCAGAGT106800.030.8034299
CAGAGTA104400.026.6968410-11
AGAGTAC99800.026.22846410-11
GAGTACT59550.025.1733812-13
GTATAGG6200.024.9971181
ATGGGGG45950.024.8641075
GTATATA6600.024.3853021
Produced by FastQC (version 0.11.5)