FastQCFastQC Report
Mon 15 Aug 2016
SRR2921525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985949
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.069.6471941
GTATCAA41800.058.664871
TCAACGC52900.046.1197434
CAACGCA54650.044.8606725
AACGCAG58450.041.8339086
ATCAACG58950.041.485363
TATCAAC61750.039.3141672
GTACATG106900.037.3595851
ACGCAGA68800.035.4523247
TACATGG112000.035.063222
ACATGGG110950.034.4305953
CGCAGAG73050.033.550078
CATGGGG92050.031.9992734
GCAGAGT80100.029.4089269
ATGGGGG52900.029.0216925
AGAGTAC73100.026.69116210-11
CAGAGTA77300.024.12509710-11
GTATAGG5800.022.5762541
TGGGGGG33250.020.5767776
GTATATA6700.020.4319691
Produced by FastQC (version 0.11.5)