##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921525_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1985949
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.918245131169027	31.0	30.0	34.0	26.0	34.0
2	30.01258642593541	31.0	30.0	34.0	25.0	34.0
3	30.10090943926556	31.0	30.0	34.0	26.0	34.0
4	33.484851322969526	37.0	35.0	37.0	27.0	37.0
5	33.51185705171684	37.0	35.0	37.0	27.0	37.0
6	33.576344609050885	37.0	35.0	37.0	28.0	37.0
7	33.45998814672482	37.0	35.0	37.0	27.0	37.0
8	33.43171148906644	37.0	35.0	37.0	27.0	37.0
9	34.91058179238238	39.0	35.0	39.0	27.0	39.0
10-11	34.79721961641512	39.0	35.0	39.0	27.0	39.0
12-13	34.643588279457326	39.0	35.0	39.0	26.5	39.0
14-15	35.81119706498002	40.0	36.0	41.0	26.0	41.0
16-17	35.738111099529746	40.0	36.0	41.0	26.0	41.0
18-19	35.75857511950206	40.0	36.0	41.0	26.0	41.0
20-21	35.77590210020499	40.0	36.0	41.0	26.0	41.0
22-23	35.74839912807428	40.0	36.0	41.0	26.0	41.0
24-25	35.71388515012218	40.0	36.0	41.0	26.0	41.0
26-27	35.63424916752646	40.0	35.5	41.0	25.5	41.0
28-29	35.59033162483024	40.0	35.0	41.0	25.5	41.0
30-31	35.49122686433539	40.0	35.0	41.0	25.0	41.0
32-33	35.389501190614666	40.0	35.0	41.0	25.0	41.0
34-35	35.22960987417099	40.0	35.0	41.0	25.0	41.0
36-37	35.09984219131508	40.0	35.0	41.0	24.0	41.0
38-39	34.97810417085232	39.5	35.0	41.0	24.0	41.0
40-41	34.822993188646834	39.0	34.5	41.0	23.5	41.0
42-43	34.63717749045922	39.0	34.0	41.0	22.5	41.0
44-45	34.45076887674357	39.0	34.0	41.0	21.5	41.0
46-47	34.211538161352586	39.0	33.0	41.0	20.0	41.0
48-49	34.06587706935073	39.0	33.0	41.0	18.5	41.0
50-51	33.87478278646632	38.0	33.0	41.0	18.0	41.0
52-53	33.68574847591756	38.0	33.0	40.0	16.5	41.0
54-55	33.48221404477154	38.0	33.0	40.0	15.0	41.0
56-57	33.24167438338044	37.5	33.0	40.0	13.5	41.0
58-59	33.001322541515414	37.0	32.5	40.0	13.0	41.0
60-61	32.692147683550786	36.5	32.0	40.0	9.5	41.0
62-63	32.38016081983979	36.0	32.0	39.5	8.0	41.0
64-65	32.07215945625995	35.5	31.5	39.0	7.5	41.0
66-67	32.02049020392769	35.0	32.0	39.0	7.0	41.0
68-69	31.76886591750342	35.0	32.0	38.5	6.0	40.5
70-71	31.472679056712934	35.0	31.5	37.5	2.0	40.0
72-73	31.143938489860513	35.0	31.0	37.0	2.0	39.0
74-75	30.841364506339286	35.0	31.0	36.5	2.0	39.0
76-77	30.558983639559727	35.0	31.0	36.0	2.0	39.0
78-79	30.287472639025474	35.0	31.0	35.5	2.0	37.0
80-81	30.045582993319567	35.0	31.0	35.0	2.0	37.0
82-83	29.847406202274076	35.0	30.5	35.0	2.0	36.5
84-85	29.644416347046175	35.0	30.0	35.0	2.0	36.0
86-87	29.4878989843143	35.0	30.0	35.0	2.0	36.0
88-89	29.360221486050246	35.0	30.0	35.0	2.0	35.5
90-91	29.248192677656878	34.5	30.0	35.0	2.0	35.0
92-93	29.1516222219201	34.0	30.0	35.0	2.0	35.0
94-95	29.084853639242496	34.0	30.0	35.0	2.0	35.0
96-97	28.9849069638747	34.0	30.0	35.0	2.0	35.0
98-99	28.922358026313866	34.0	30.0	35.0	2.0	35.0
100-101	28.85972348736045	34.0	30.0	35.0	2.0	35.0
102-103	27.886916532096244	33.5	28.0	34.5	2.0	35.0
104-105	28.70050414184856	34.0	29.5	35.0	2.0	35.0
106-107	29.01958433977912	35.0	30.0	35.0	2.0	35.0
108-109	29.057502987236834	35.0	30.0	35.0	2.0	35.0
110-111	29.000939852936806	35.0	30.0	35.0	2.0	35.0
112-113	28.92874942911424	35.0	30.0	35.0	2.0	35.0
114-115	28.81136046293233	35.0	30.0	35.0	2.0	35.0
116-117	28.74560877444486	35.0	30.0	35.0	2.0	35.0
118-119	28.582727955249606	35.0	30.0	35.0	2.0	35.0
120-121	28.413109551151614	35.0	30.0	35.0	2.0	35.0
122-123	28.2863031729415	35.0	30.0	35.0	2.0	35.0
124-125	27.447374277990022	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69741.0
3	12607.0
4	8446.0
5	7294.0
6	7677.0
7	8938.0
8	9834.0
9	10341.0
10	10879.0
11	11267.0
12	11469.0
13	12069.0
14	13444.0
15	11743.0
16	8621.0
17	7601.0
18	7009.0
19	6517.0
20	6362.0
21	6643.0
22	7842.0
23	10588.0
24	15181.0
25	20624.0
26	24843.0
27	26815.0
28	30958.0
29	37098.0
30	41916.0
31	49821.0
32	63107.0
33	82131.0
34	114677.0
35	180843.0
36	347223.0
37	527578.0
38	155984.0
39	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.864612796224044	17.044068718690102	12.10641775744952	24.98490072763633
2	16.721717879908777	19.408650242970772	38.33604308198148	25.53358879513897
3	18.991368589967983	24.413487705138905	28.949448052127934	27.645695652765177
4	13.33388740297485	15.19666953775481	35.66747762314198	35.80196543612836
5	14.37053178130341	37.35031640516585	33.36975153518084	14.909400278349908
6	33.505419245310584	34.56851321499908	17.42944150162086	14.496626038069474
7	29.71934464216001	31.33325777324417	20.75005981840392	18.197337766191897
8	27.95308847314898	33.31120367063607	19.613916300511416	19.121791555703535
9	28.053942644386332	14.392747339536047	18.736073110879193	38.817236905198435
10-11	26.43614183885593	24.74614245841995	27.002312535291225	21.815403167432894
12-13	26.639528508288656	22.469271866067825	27.113367503268442	23.777832122375077
14-15	24.110794279707225	23.578804633898542	24.39896478235093	27.911436304043303
16-17	24.274051472171607	26.631717204026202	24.845214513555614	24.24901681024658
18-19	24.382664505460323	25.846944777147634	26.311201119394056	23.459189597997987
20-21	25.568766667489594	24.850373618799495	25.887668992536696	23.69319072117422
22-23	25.31856848850947	24.415069814776096	25.7807339934858	24.485627703228634
24-25	24.68374983152387	24.87047806913127	25.829286757083242	24.616485342261623
26-27	24.380950509793607	24.97039071162543	25.94083482324351	24.707823955337453
28-29	24.468132000442317	25.09067341525238	25.897999932997767	24.543194651307537
30-31	24.881322358348797	24.936511303028364	25.752708187762313	24.42945815086053
32-33	24.469770748303777	25.059916396662917	25.702761267985625	24.767551587047677
34-35	24.758393056610238	25.10709891585094	25.79944816573666	24.335059861802165
36-37	24.86409610718252	24.899462228403177	25.719432298185772	24.517009366228528
38-39	24.86616123128392	25.046283991873064	25.56345044671704	24.524104330125972
40-41	24.82935862823934	24.97049134740311	25.76849700077229	24.43165302358526
42-43	24.74947004611197	24.961426410407878	25.644100224577016	24.645003318903136
44-45	24.59963070350227	25.046539923571242	25.643107431021413	24.710721941905074
46-47	24.68177685495494	25.102665020432234	25.549299741551945	24.66625838306088
48-49	24.811368541852065	25.241716525488517	25.29723229698016	24.64968263567926
50-51	24.766419643621816	25.39358277455075	25.251439568356197	24.588558013471236
52-53	24.874206405787184	25.209555224483687	25.392017025401564	24.52422134432756
54-55	24.61103791147717	25.147571217674443	25.50312748969363	24.738263381154763
56-57	24.642542386499795	25.250573587013825	25.2729906445307	24.83389338195568
58-59	24.79237507997199	25.152034940833325	25.30427138589571	24.751318593298976
60-61	24.776728429590094	25.26000393390139	25.504978702280983	24.458288934227536
62-63	24.636951460203694	25.350480118722462	25.41206286326263	24.60050555781121
64-65	24.64983525066532	25.067463857658183	25.52058119918172	24.762119692494775
66-67	24.625309623741828	25.26632993707413	25.44368594235804	24.664674496826002
68-69	24.61658880958371	25.2520124097869	25.38637033880612	24.74502844182327
70-71	24.529790414825246	25.090976414268567	25.555587741515396	24.82364542939079
72-73	24.744490767114495	25.36627061539306	25.324766577563945	24.564472039928496
74-75	24.541770455799053	25.470067172242526	25.302804006659297	24.685358365299127
76-77	24.595213660145944	25.089965811858185	25.38036983636284	24.934450691633035
78-79	24.538686979575523	25.20479505132248	25.44266380770836	24.813854161393635
80-81	24.57915951268477	25.43836763803009	25.18722069251384	24.795252156771298
82-83	24.80214403868657	25.33432614901754	25.24241431487786	24.62111549741803
84-85	24.504450240554224	25.434662068277813	25.165401488600807	24.895486202567156
86-87	24.3510991742155	25.53661891180972	25.31444727534426	24.797834638630516
88-89	24.4571615640798	25.321648692717876	25.31976001158391	24.90142973161841
90-91	24.51811124182164	25.2426314177974	25.363292454958586	24.875964885422373
92-93	24.56904496900166	25.43171047396492	25.25760605160179	24.741638505431634
94-95	24.466236504988824	25.207843630984232	25.351018224166694	24.97490163986025
96-97	24.424458602062003	25.19079798503227	25.40865138872732	24.976092024178403
98-99	24.39768470817634	25.30250474127208	25.218581959296067	25.08122859125551
100-101	24.332877837813882	25.348741117648526	25.36629116613148	24.952089878406113
102-103	24.425431480312092	25.13566061279923	25.533754515725853	24.905153391162823
104-105	24.338043105785953	25.29753849198486	25.38447922801259	24.979939174216597
106-107	24.331113805263165	25.253596504148867	25.40731424514014	25.007975445447826
108-109	24.3228727328581	25.21774382398191	25.42187787209077	25.037505571069218
110-111	24.27366997805695	25.444167341240213	25.38809485742054	24.894067823282292
112-113	24.286113908047803	25.415404897815925	25.476966880157075	24.821514313979204
114-115	24.117324325351895	25.616699008885362	25.43259133812688	24.833385327635867
116-117	24.100501783775986	25.556654180612448	25.427387388203993	24.915456647407577
118-119	24.318978450007776	25.285780195328982	25.360563090211947	25.034678264451294
120-121	24.024145967421795	25.379292778572765	25.615986734828734	24.980574519176706
122-123	24.02204612965008	25.521908246899528	25.467586220791205	24.988459402659185
124-125	24.002092773937616	25.55029732395849	25.48538910438239	24.962220797721514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	187.5
2	233.5
3	253.5
4	225.5
5	197.5
6	198.0
7	250.5
8	321.5
9	371.5
10	398.5
11	413.5
12	458.5
13	505.0
14	604.0
15	718.0
16	813.0
17	916.5
18	1028.0
19	1133.5
20	1260.0
21	1473.0
22	1746.5
23	2143.5
24	2654.5
25	3404.0
26	4531.5
27	5860.0
28	7168.5
29	8884.5
30	11254.5
31	13438.0
32	16040.0
33	18792.0
34	21497.5
35	24494.5
36	28277.0
37	32277.5
38	35064.0
39	38273.0
40	41970.5
41	44954.0
42	48024.0
43	53486.5
44	59073.5
45	63524.5
46	68361.5
47	72338.0
48	77723.0
49	83082.5
50	83518.5
51	83328.5
52	83781.5
53	84073.5
54	85094.0
55	83860.5
56	79093.0
57	72670.0
58	67827.5
59	62679.0
60	55905.0
61	49482.5
62	42789.5
63	33743.0
64	25380.5
65	19917.5
66	15416.0
67	11991.5
68	9461.0
69	7521.0
70	6393.5
71	5239.0
72	4071.0
73	3589.0
74	2941.5
75	2103.0
76	1434.5
77	854.0
78	481.0
79	300.0
80	178.0
81	91.5
82	64.0
83	39.5
84	27.5
85	23.0
86	20.0
87	20.0
88	17.5
89	13.0
90	12.0
91	10.5
92	13.0
93	11.5
94	6.5
95	6.0
96	8.0
97	9.5
98	8.0
99	9.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037966735298842015
2	0.02643572418022819
3	0.026486077940571482
4	0.03217605285936346
5	0.03413984951275184
6	0.04501626174690286
7	0.038973810505707855
8	0.034240557033438423
9	0.029255534759452532
10-11	0.034341264554125006
12-13	0.03401396511189361
14-15	0.03353560438863234
16-17	0.035323162880819194
18-19	0.04765983416492568
20-21	0.05901460712233799
22-23	0.05307286340182955
24-25	0.06276596226791323
26-27	0.046828997119261376
28-29	0.047357711602865935
30-31	0.0478360723261272
32-33	0.055389136377620975
34-35	0.03784085089798379
36-37	0.05027822970277686
38-39	0.05999650544903218
40-41	0.047231827202007706
42-43	0.055716435819852375
44-45	0.05508701381556123
46-47	0.061305703217957766
48-49	0.04670311271840314
50-51	0.04929633137608267
52-53	0.05790682439478557
54-55	0.04484002358570134
56-57	0.04297693445299955
58-59	0.04426095534175349
60-61	0.03235229102056498
62-63	0.04103831467978281
64-65	0.04088725339875294
66-67	0.034064318872236904
68-69	0.029230357879280886
70-71	0.0481633717683586
72-73	0.030212256205975077
74-75	0.037966735298842015
76-77	0.030791324449922934
78-79	0.030740970689579643
80-81	0.023062022237227642
82-83	0.017648993000323774
84-85	0.016415325871913126
86-87	0.016213910830539958
88-89	0.021979416389846868
90-91	0.011128181035867487
92-93	0.018076999963241757
94-95	0.013167508329770804
96-97	0.010574289672091277
98-99	0.0098189832669419
100-101	0.010045575188486713
102-103	0.005589267398105389
104-105	0.005715151798963619
106-107	0.008383901097158085
108-109	0.0122863175237632
110-111	0.006369750683426411
112-113	0.007326472129948956
114-115	0.007779655973038584
116-117	0.006722227005829455
118-119	0.24577670423560727
120-121	0.3642339254432012
122-123	0.028525405234474804
124-125	0.0034240557033438424
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1985949.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.960213996700645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47022366494959	45.26640946321193
2	10.551272426299336	12.020054706789942
3	3.666610000116735	6.265526707472755
4	1.804029976026231	4.1103173396366754
5	1.020281681472572	2.9057731456795635
6	0.6871997188801818	2.348582582753261
7	0.4788348005785526	1.9092172897015285
8	0.35685723730888935	1.6261331682708582
9	0.27384813502771	1.4038603536398162
>10	1.5809748904075742	16.1267670700168
>50	0.08410156458406624	3.294012625471751
>100	0.025053029922040785	2.3722604168175603
>500	4.4554651665306246E-4	0.1491743817623047
>1k	2.6732790999183653E-4	0.2019107487752453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.03537603432918E-5
7	0.0	0.0	0.0	0.0	5.03537603432918E-5
8	0.0	0.0	0.0	0.0	5.03537603432918E-5
9	0.0	0.0	0.0	0.0	5.03537603432918E-5
10-11	0.0	0.0	0.0	0.0	5.03537603432918E-5
12-13	0.0	0.0	0.0	0.0	7.55306405149377E-5
14-15	0.0	0.0	0.0	0.0	1.258844008582295E-4
16-17	0.0	0.0	0.0	0.0	1.510612810298754E-4
18-19	0.0	0.0	0.0	0.0	1.510612810298754E-4
20-21	0.0	0.0	0.0	7.55306405149377E-5	2.51768801716459E-4
22-23	0.0	0.0	0.0	1.510612810298754E-4	2.769456818881049E-4
24-25	0.0	0.0	0.0	2.014150413731672E-4	3.021225620597508E-4
26-27	0.0	0.0	0.0	2.769456818881049E-4	3.524763224030426E-4
28-29	5.03537603432918E-5	0.0	0.0	9.567214465225441E-4	3.524763224030426E-4
30-31	5.03537603432918E-5	0.0	0.0	0.0038017089059185303	3.524763224030426E-4
32-33	5.03537603432918E-5	0.0	0.0	0.009214738142822398	3.524763224030426E-4
34-35	5.03537603432918E-5	0.0	0.0	0.016893686595174397	3.524763224030426E-4
36-37	5.03537603432918E-5	0.0	0.0	0.027619037548295554	3.524763224030426E-4
38-39	5.03537603432918E-5	0.0	0.0	0.04866690937179152	3.524763224030426E-4
40-41	5.03537603432918E-5	0.0	0.0	0.07326472129948956	4.028300827463344E-4
42-43	5.03537603432918E-5	0.0	0.0	0.09627638977637391	4.2800696291798025E-4
44-45	5.03537603432918E-5	0.0	0.0	0.1212770317868183	4.783607232612721E-4
46-47	5.03537603432918E-5	0.0	0.0	0.15010455958335284	5.287144836045638E-4
48-49	5.03537603432918E-5	0.0	0.0	0.18160083667808186	6.545988844627933E-4
50-51	5.03537603432918E-5	0.0	0.0	0.2163449313149532	7.049526448060852E-4
52-53	5.03537603432918E-5	0.0	0.0	0.2540095440517355	9.063676861792522E-4
54-55	5.03537603432918E-5	0.0	0.0	0.29134685734628635	9.8189832669419E-4
56-57	5.03537603432918E-5	0.0	0.0	0.3334174241131066	0.001007075206865836
58-59	5.03537603432918E-5	0.0	0.0	0.38273893236936096	0.001007075206865836
60-61	5.03537603432918E-5	0.0	0.0	0.4368692247383996	0.0010574289672091277
62-63	5.03537603432918E-5	0.0	0.0	0.4975706828322379	0.0010574289672091277
64-65	5.03537603432918E-5	0.0	0.0	0.5564594055537176	0.0011077827275524194
66-67	5.03537603432918E-5	0.0	0.0	0.6209122187931312	0.0011077827275524194
68-69	5.03537603432918E-5	0.0	0.0	0.6968205125106435	0.0011581364878957114
70-71	5.03537603432918E-5	0.0	0.0	0.781037176684799	0.0011581364878957114
72-73	5.03537603432918E-5	0.0	0.0	0.8717242990630676	0.001460259049955462
74-75	5.03537603432918E-5	0.0	0.0	0.9631163740861421	0.0015357896904703997
76-77	5.03537603432918E-5	0.0	0.0	1.061532798677106	0.0015861434508136916
78-79	5.03537603432918E-5	0.0	0.0	1.1759868959374082	0.0016113203309853375
80-81	5.03537603432918E-5	0.0	0.0	1.3033063789654216	0.0016113203309853375
82-83	5.03537603432918E-5	0.0	0.0	1.438858701809563	0.0018379122525301506
84-85	5.03537603432918E-5	0.0	0.0	1.5749649160174808	0.0018630891327017964
86-87	5.03537603432918E-5	0.0	0.0	1.7212425898147434	0.0018630891327017964
88-89	5.03537603432918E-5	0.0	0.0	1.885798678616621	0.0018882660128734423
90-91	5.03537603432918E-5	0.0	0.0	2.0758085932720327	0.0019134428930450882
92-93	5.03537603432918E-5	0.0	0.0	2.2777523491288045	0.0020645041740749636
94-95	5.03537603432918E-5	0.0	0.0	2.4861917400698608	0.0020645041740749636
96-97	5.03537603432918E-5	2.51768801716459E-5	0.0	2.700900174173657	0.0022155654551048393
98-99	5.03537603432918E-5	5.03537603432918E-5	0.0	2.934617152807046	0.002316272975791423
100-101	5.03537603432918E-5	5.03537603432918E-5	0.0	3.203304817998851	0.002316272975791423
102-103	5.03537603432918E-5	5.03537603432918E-5	0.0	3.5019026168345713	0.002467334256821298
104-105	5.03537603432918E-5	5.03537603432918E-5	0.0	3.814272169124182	0.0025176880171645898
106-107	5.03537603432918E-5	5.03537603432918E-5	0.0	4.132910764576533	0.0025428648973362354
108-109	5.03537603432918E-5	5.03537603432918E-5	0.0	4.468644461665431	0.0025680417775078815
110-111	1.007075206865836E-4	5.03537603432918E-5	0.0	4.8346407687206465	0.0026183955378511732
112-113	1.007075206865836E-4	5.03537603432918E-5	0.0	5.229817079894801	0.0026687492981944654
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2145	0.0	69.647194	1
GTATCAA	4180	0.0	58.66487	1
TCAACGC	5290	0.0	46.119743	4
CAACGCA	5465	0.0	44.860672	5
AACGCAG	5845	0.0	41.833908	6
ATCAACG	5895	0.0	41.48536	3
TATCAAC	6175	0.0	39.314167	2
GTACATG	10690	0.0	37.359585	1
ACGCAGA	6880	0.0	35.452324	7
TACATGG	11200	0.0	35.06322	2
ACATGGG	11095	0.0	34.430595	3
CGCAGAG	7305	0.0	33.55007	8
CATGGGG	9205	0.0	31.999273	4
GCAGAGT	8010	0.0	29.408926	9
ATGGGGG	5290	0.0	29.021692	5
AGAGTAC	7310	0.0	26.691162	10-11
CAGAGTA	7730	0.0	24.125097	10-11
GTATAGG	580	0.0	22.576254	1
TGGGGGG	3325	0.0	20.576777	6
GTATATA	670	0.0	20.431969	1
>>END_MODULE