FastQCFastQC Report
Mon 15 Aug 2016
SRR2921525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985949
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.17447577958950608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29600.14904713061614372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.080.263741
GTATCAA61700.059.908861
TCAACGC81950.044.5342064
CAACGCA83150.043.8011365
ATCAACG85850.042.5132453
AACGCAG86450.042.3948866
TATCAAC88300.041.6044242
GTACATG105150.036.740941
ACGCAGA100050.036.4509057
ACATGGG110150.034.377693
TACATGG111650.033.863362
CGCAGAG108600.033.6907168
CATGGGG91550.032.125574
GCAGAGT114800.030.9894089
ATGGGGG49500.029.0941965
AGAGTAC107100.027.13495610-11
CAGAGTA112300.026.67311510-11
GTATAGG5600.025.5509911
GAGTACT61850.024.19030612-13
AGTACTT64800.023.59397912-13
Produced by FastQC (version 0.11.5)