##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921525_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1985949
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.390298038872096	33.0	31.0	34.0	28.0	34.0
2	31.457214158067504	33.0	31.0	34.0	28.0	34.0
3	31.54803421437308	33.0	31.0	34.0	28.0	34.0
4	35.06641056744156	37.0	35.0	37.0	32.0	37.0
5	34.74528499976586	37.0	35.0	37.0	32.0	37.0
6	34.71663421366812	37.0	35.0	37.0	32.0	37.0
7	34.56938269814582	37.0	35.0	37.0	31.0	37.0
8	34.535244862783486	37.0	35.0	37.0	31.0	37.0
9	36.142380292746694	39.0	37.0	39.0	31.0	39.0
10-11	36.01539918698819	39.0	37.0	39.0	30.0	39.0
12-13	35.918793987156775	39.0	37.0	39.0	30.0	39.0
14-15	37.11974073855875	40.0	37.0	41.0	31.0	41.0
16-17	37.09981097198367	40.0	37.0	41.0	31.0	41.0
18-19	37.11776359815887	40.0	37.0	41.0	30.5	41.0
20-21	37.0949890958932	40.0	37.0	41.0	30.0	41.0
22-23	37.035234036725015	40.0	37.0	41.0	30.0	41.0
24-25	36.969144978043246	40.0	37.0	41.0	30.0	41.0
26-27	36.84544013970147	40.0	37.0	41.0	30.0	41.0
28-29	36.6634888408514	40.0	36.0	41.0	30.0	41.0
30-31	36.61741842313171	40.0	36.0	41.0	30.0	41.0
32-33	36.4939124821433	40.0	36.0	41.0	30.0	41.0
34-35	36.36850065132589	40.0	35.5	41.0	30.0	41.0
36-37	36.24038306119644	40.0	35.0	41.0	29.0	41.0
38-39	36.06937967692021	40.0	35.0	41.0	28.5	41.0
40-41	35.87298767491008	40.0	35.0	41.0	27.5	41.0
42-43	35.70442821039211	40.0	35.0	41.0	27.0	41.0
44-45	35.490004526803055	39.0	35.0	41.0	26.5	41.0
46-47	35.220099055917345	39.0	35.0	41.0	25.5	41.0
48-49	34.96436011196663	39.0	35.0	41.0	24.0	41.0
50-51	34.744033457052524	39.0	34.0	41.0	24.0	41.0
52-53	34.541981692379814	38.5	34.0	41.0	23.0	41.0
54-55	34.32488120289091	38.0	34.0	40.5	23.0	41.0
56-57	34.06454722654006	38.0	34.0	40.0	22.5	41.0
58-59	33.913202705608256	37.0	33.5	40.0	22.5	41.0
60-61	33.79409617266103	37.0	34.0	40.0	22.0	41.0
62-63	33.522820827725184	36.5	33.0	40.0	22.0	41.0
64-65	33.22480990196627	36.0	33.0	39.5	21.5	41.0
66-67	32.91991033002358	35.5	33.0	39.0	21.0	41.0
68-69	32.60330804063951	35.0	33.0	39.0	20.0	41.0
70-71	32.29950542536591	35.0	33.0	37.5	20.0	40.0
72-73	31.98140309746121	35.0	32.0	37.0	19.5	39.5
74-75	31.69604657521417	35.0	32.0	36.5	18.5	39.0
76-77	31.40464533580671	35.0	32.0	36.0	18.0	39.0
78-79	31.12931978615765	35.0	32.0	36.0	17.5	37.5
80-81	30.90875974156436	35.0	32.0	35.0	16.5	37.0
82-83	30.72231487314125	35.0	32.0	35.0	15.0	36.5
84-85	30.53142779598066	35.0	31.0	35.0	12.5	36.0
86-87	30.391718266682577	35.0	31.0	35.0	11.0	36.0
88-89	30.290390135899763	35.0	31.0	35.0	10.5	36.0
90-91	30.190753891464485	35.0	31.0	35.0	10.0	35.0
92-93	30.251090033027033	35.0	31.5	35.0	10.0	35.0
94-95	30.222557074728506	35.0	32.0	35.0	7.5	35.0
96-97	30.20232770327939	35.0	32.0	35.0	7.0	35.0
98-99	30.161038375104297	35.0	32.0	35.0	7.0	35.0
100-101	30.111522501333116	35.0	32.0	35.0	6.0	35.0
102-103	29.230427619238963	34.0	29.5	35.0	2.0	35.0
104-105	29.935606100660188	35.0	31.0	35.0	2.0	35.0
106-107	30.042215585596608	35.0	32.0	35.0	2.0	35.0
108-109	30.050472595217702	35.0	32.0	35.0	2.0	35.0
110-111	30.017824979392724	35.0	32.0	35.0	2.0	35.0
112-113	29.971811713191023	35.0	32.0	35.0	2.0	35.0
114-115	29.90986979021113	35.0	32.0	35.0	2.0	35.0
116-117	29.833268880520095	35.0	32.0	35.0	2.0	35.0
118-119	29.741969204647248	35.0	32.0	35.0	2.0	35.0
120-121	29.62124077707937	35.0	31.5	35.0	2.0	35.0
122-123	29.503244041010117	35.0	31.5	35.0	2.0	35.0
124-125	28.593073638849738	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28101.0
3	10820.0
4	6214.0
5	5326.0
6	5604.0
7	6579.0
8	7664.0
9	8570.0
10	10044.0
11	11160.0
12	11619.0
13	13251.0
14	16833.0
15	14532.0
16	8121.0
17	6853.0
18	6089.0
19	5685.0
20	5395.0
21	5910.0
22	6798.0
23	9004.0
24	12725.0
25	17407.0
26	22097.0
27	24645.0
28	29325.0
29	35183.0
30	40569.0
31	48726.0
32	60773.0
33	78611.0
34	109587.0
35	174658.0
36	350462.0
37	580686.0
38	189907.0
39	416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12194691577175	17.43263388902697	12.403965259862861	25.041453935338414
2	16.813207010370533	19.78428079751651	37.54878733480537	25.853724857307586
3	18.861910230065675	24.5265723840606	29.285527217051456	27.325990168822273
4	13.415419991707028	15.455813721494167	35.42368978840828	35.70507649839053
5	14.18894449987635	37.363750422454906	33.3221350254027	15.125170052266046
6	33.504556234862285	34.976512461968426	16.861670060402282	14.657261242767012
7	29.373795101236677	31.67355276172365	20.57785786374597	18.374794273293702
8	28.03315654943067	33.39720180557262	19.211048960345483	19.358592684651224
9	28.11803989282285	13.985701991720509	18.63081740959005	39.265440705866595
10-11	26.50287615777751	24.666118443415254	26.864567538972782	21.966437859834457
12-13	26.777486915608538	22.42637126461931	27.229029661478904	23.567112158293252
14-15	24.251622441457155	23.374965254978104	24.79956150775267	27.573850795812067
16-17	24.080601629164722	26.37782913838483	25.20134166506567	24.34022756738478
18-19	24.191543462918524	25.245406658604573	27.270850344426645	23.29219953405026
20-21	25.27257472702239	24.21493442594881	27.034074736590835	23.478416110437962
22-23	25.021710792851216	23.8961487609174	26.898646288946292	24.183494157285097
24-25	24.485034759083593	24.349941682093885	26.91890084344412	24.2461227153784
26-27	24.11931420468108	24.386589263071173	27.163203211631487	24.330893320616262
28-29	24.26427466911151	24.56523003621184	27.004499474108695	24.165995820567954
30-31	24.60333009322448	24.40270356025847	26.858546584539138	24.13541976197791
32-33	24.2442585709113	24.52511684802178	26.809551699426752	24.421072881640168
34-35	24.412685405971114	24.624491605942257	26.916939283313894	24.045883704772727
36-37	24.616551440727132	24.453583284986784	26.707287839231615	24.222577435054472
38-39	24.6591662551488	24.64294896767144	26.55419658827556	24.143688188904203
40-41	24.57497914521702	24.532224636920304	26.651443241627682	24.24135297623499
42-43	24.556484325310798	24.61792111013249	26.44478419322171	24.380810371335006
44-45	24.49819362042182	24.63709359607152	26.36792931512189	24.49678346838477
46-47	24.598958624485874	24.727128693325035	26.10330759356037	24.57060508862872
48-49	24.783579080859848	24.967131116187254	25.607208717223344	24.642081085729554
50-51	24.666971857478913	25.212311578667464	25.548676171899988	24.57204039195364
52-53	24.891741206333855	25.082472097989445	25.576926951126154	24.448859744550546
54-55	24.592204019615103	25.00751657044953	25.739330876661263	24.66094853327411
56-57	24.59428471851311	25.098934217527606	25.521565581896883	24.785215482062405
58-59	24.638789493431506	24.97369832520403	25.693450140132907	24.694062041231557
60-61	24.752198746652713	25.022575460964973	25.823985438890634	24.40124035349168
62-63	24.59189491105092	25.145830961995813	25.742299994258428	24.519974132694845
64-65	24.57144986740914	24.822303568231476	25.88698388588549	24.719262678473896
66-67	24.605642012813945	25.052959179947905	25.720800059424064	24.62059874781409
68-69	24.662565254508323	25.036779241448603	25.580799750188238	24.719855753854834
70-71	24.53199628538104	24.85377716718001	25.880434191415684	24.73379235602326
72-73	24.795791895334247	25.134254332576045	25.47578889371811	24.594164878371593
74-75	24.588767808016517	25.313364432441283	25.377375713737116	24.720492045805084
76-77	24.567385351421105	24.95483302382676	25.470372352145038	25.007409272607095
78-79	24.538889244266432	25.017843030691022	25.573835392816147	24.869432332226403
80-81	24.61389924929156	25.210290782108203	25.34224583982721	24.833564128773027
82-83	24.680595307248165	25.15116973840554	25.340265676015463	24.827969278330837
84-85	24.515570363569935	25.192053326981284	25.294736924271227	24.997639385177557
86-87	24.338593163504953	25.30983242522009	25.38846926578673	24.963105145488225
88-89	24.43762745138528	25.034889383907117	25.41611665418489	25.11136651052272
90-91	24.45389700187238	25.017273453371242	25.44898388784644	25.07984565690993
92-93	24.468385645820735	25.197767539484268	25.362487794536282	24.97135902015872
94-95	24.373780721782488	25.002769656250408	25.423228653755704	25.200220968211397
96-97	24.373963319512608	24.949191955711903	25.439950329975336	25.236894394800157
98-99	24.28187330414945	25.02543539786602	25.32350981047261	25.369181487511916
100-101	24.24428219776324	25.091956199439196	25.4464609453057	25.217300657491865
102-103	24.34206567472022	24.895670387158223	25.501594118717847	25.26066981940371
104-105	24.297329354292035	25.037193360912692	25.335445216300574	25.330032068494702
106-107	24.27302259805216	24.91561324316302	25.382166831869252	25.429197326915563
108-109	24.21858022022408	24.88247068786597	25.441454172948454	25.457494918961498
110-111	24.232370201534845	25.081391153635728	25.33756777980341	25.348670865026012
112-113	24.166477193899503	25.08234196901107	25.422209531953165	25.32897130513626
114-115	24.0381911232981	25.212415589555704	25.389438022384237	25.359955264761965
116-117	23.980293115607864	25.11789308458767	25.365928703419044	25.535885096385417
118-119	23.991488399532575	24.81583252360997	25.47484956015592	25.717829516701535
120-121	23.782977956283187	24.964801356784324	25.598024444677197	25.65419624225529
122-123	23.791004289917776	25.021859974807843	25.45276520813491	25.73437052713948
124-125	23.75216039192903	25.11695643556926	25.3838968924857	25.746986280016003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	58.5
2	96.0
3	144.5
4	120.5
5	107.5
6	179.0
7	396.5
8	680.5
9	785.5
10	657.5
11	562.0
12	615.5
13	714.5
14	854.0
15	996.0
16	1167.5
17	1378.0
18	1563.5
19	1715.5
20	1903.5
21	2159.0
22	2523.5
23	2974.0
24	3381.5
25	4130.5
26	5315.0
27	6564.0
28	7765.5
29	9287.0
30	11405.5
31	13640.0
32	16315.5
33	18818.5
34	21185.5
35	24035.0
36	27536.0
37	31168.0
38	33894.0
39	37181.0
40	41032.5
41	43986.0
42	46823.5
43	52460.5
44	58304.5
45	62743.0
46	67700.0
47	72000.5
48	77694.5
49	82987.5
50	83823.0
51	84228.5
52	84681.0
53	84888.0
54	85611.0
55	83759.0
56	79145.5
57	72854.0
58	68165.5
59	63286.0
60	56083.5
61	49433.0
62	42391.0
63	33269.0
64	24948.5
65	19450.0
66	15160.5
67	11695.0
68	9206.0
69	7332.0
70	6156.0
71	5060.5
72	4006.0
73	3582.0
74	2891.0
75	2050.5
76	1374.0
77	768.5
78	417.0
79	250.5
80	131.0
81	51.5
82	24.0
83	12.0
84	8.5
85	6.0
86	2.5
87	1.5
88	1.5
89	1.5
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12195680755145272
2	0.0017120278516719212
3	0.02160176318727218
4	0.05720187174997948
5	0.026687492981944654
6	0.02155140942692889
7	0.015357896904703999
8	0.004884314753299305
9	0.00392759330677676
10-11	0.007049526448060851
12-13	0.004557015311067908
14-15	0.002668749298194465
16-17	0.010448405271233049
18-19	0.005664798038620327
20-21	0.012840208887539409
22-23	0.009340622543680628
24-25	0.014149406656464993
26-27	0.00840907797732973
28-29	0.013721399693547015
30-31	0.021098225583839263
32-33	0.012588440085822949
34-35	0.020519157339891406
36-37	0.014350821697838162
38-39	0.020821279901951158
40-41	0.009743452626426964
42-43	0.008459431737673022
44-45	0.017497931719293897
46-47	0.01540825066504729
48-49	0.020569511100234698
50-51	0.01540825066504729
52-53	0.02160176318727218
54-55	0.016918863475346043
56-57	0.020770926141607866
58-59	0.01681815595465946
60-61	0.019436551492510633
62-63	0.021702470707958763
64-65	0.016591564033114646
66-67	0.011153357916039133
68-69	0.022860607195854477
70-71	0.014149406656464993
72-73	0.01772452364083871
74-75	0.018202884364099985
76-77	0.013167508329770804
78-79	0.02920518099910924
80-81	0.021375171265727366
82-83	0.009290268783337337
84-85	0.0119590180815318
86-87	0.012009371841875094
88-89	0.01953725901319722
90-91	0.012235963763419908
92-93	0.007955894134240105
94-95	0.007200587729090727
96-97	0.002165211694761547
98-99	0.0017875584921868588
100-101	0.011128181035867487
102-103	0.0027946336990526946
104-105	0.002190388574933193
106-107	1.762381612015213E-4
108-109	0.019159605810622527
110-111	7.30129524977731E-4
112-113	0.00883708494024771
114-115	0.0021400348145899015
116-117	0.007326472129948956
118-119	0.009491683824710504
120-121	0.003877239546433468
122-123	6.797757646344393E-4
124-125	0.024547458167354753
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1985949.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.57642351673691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98081006412917	43.89470949819
2	10.812984961263556	12.018940633745808
3	3.6164026062927124	6.029601685630649
4	1.840994664448202	4.092635966537049
5	1.0980291140745966	3.0512265538758596
6	0.7142501938370355	2.381728276175911
7	0.4837494234757355	1.8819543984545353
8	0.39920512935320085	1.7749114671189785
9	0.2789969917311635	1.3955089475112017
>10	1.6534161766044335	16.589080720840624
>50	0.09019225951264086	3.4241194710317377
>100	0.030151497287069094	2.8518061663058774
>500	4.5384332806005227E-4	0.1416523062830288
>1k	3.6307466244804093E-4	0.47212390829874057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3465	0.17447577958950608	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2960	0.14904713061614372	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.007075206865836E-4	0.0
5	0.0	0.0	0.0	1.007075206865836E-4	0.0
6	0.0	0.0	0.0	1.007075206865836E-4	0.0
7	0.0	0.0	0.0	1.007075206865836E-4	0.0
8	0.0	0.0	0.0	1.007075206865836E-4	0.0
9	0.0	0.0	0.0	1.007075206865836E-4	0.0
10-11	0.0	0.0	0.0	1.007075206865836E-4	0.0
12-13	0.0	0.0	0.0	1.007075206865836E-4	1.762381612015213E-4
14-15	0.0	0.0	0.0	1.007075206865836E-4	3.2729944223139666E-4
16-17	0.0	0.0	0.0	1.007075206865836E-4	5.03537603432918E-4
18-19	0.0	0.0	0.0	1.007075206865836E-4	5.03537603432918E-4
20-21	0.0	0.0	0.0	1.762381612015213E-4	5.538913637762097E-4
22-23	0.0	0.0	0.0	2.51768801716459E-4	5.538913637762097E-4
24-25	0.0	0.0	0.0	3.021225620597508E-4	5.538913637762097E-4
26-27	0.0	0.0	0.0	3.776532025746885E-4	5.790682439478557E-4
28-29	0.0	0.0	0.0	0.0010574289672091277	6.042451241195016E-4
30-31	0.0	0.0	0.0	0.003877239546433468	6.042451241195016E-4
32-33	0.0	0.0	0.0	0.009416153184195565	6.042451241195016E-4
34-35	0.0	0.0	0.0	0.017170632277062503	6.545988844627933E-4
36-37	0.0	0.0	0.0	0.028399520833616576	6.545988844627933E-4
38-39	0.0	0.0	0.0	0.04992575338037382	6.797757646344392E-4
40-41	0.0	0.0	0.0	0.0745990959485868	7.049526448060852E-4
42-43	0.0	0.0	0.0	0.09788771010735925	7.049526448060852E-4
44-45	0.0	0.0	0.0	0.12354295100226642	7.049526448060852E-4
46-47	0.0	0.0	0.0	0.15294954704274882	8.056601654926688E-4
48-49	0.0	0.0	0.0	0.18484865422022417	8.056601654926688E-4
50-51	0.0	0.0	0.0	0.22022217086138668	8.056601654926688E-4
52-53	0.0	0.0	0.0	0.25821408304040033	8.308370456643146E-4
54-55	0.0	0.0	0.0	0.2959794032978692	9.063676861792524E-4
56-57	0.0	0.0	0.0	0.3393339909534434	9.063676861792524E-4
58-59	0.0	0.0	0.0	0.3896877512967352	9.063676861792524E-4
60-61	0.0	0.0	0.0	0.44517759519504274	0.001007075206865836
62-63	0.0	0.0	0.0	0.5070120128966051	0.0011077827275524194
64-65	0.0	0.0	0.0	0.5670588721059806	0.0011077827275524194
66-67	0.0	0.0	0.0	0.6329467675151779	0.0011077827275524194
68-69	0.0	0.0	0.0	0.7097362520386978	0.0011077827275524194
70-71	0.0	0.0	0.0	0.7956397671843536	0.0012084902482390031
72-73	0.0	0.0	0.0	0.8875605566910328	0.0015609665706420458
74-75	0.0	0.0	0.0	0.9807150133261227	0.0016868509715002751
76-77	0.0	0.0	0.0	1.0823289016988855	0.001712027851671921
78-79	0.0	0.0	0.0	1.1982180811289715	0.001712027851671921
80-81	0.0	0.0	0.0	1.3277279527319181	0.001712027851671921
82-83	0.0	0.0	0.0	1.4655461947915076	0.001712027851671921
84-85	0.0	0.0	0.0	1.6054037641450007	0.001712027851671921
86-87	0.0	0.0	0.0	1.7537459421163382	0.0017875584921868588
88-89	0.0	0.0	0.0	1.9214491409396715	0.0019134428930450882
90-91	0.0	0.0	0.0	2.1154873564225465	0.0019134428930450882
92-93	0.0	0.0	0.0	2.322441311433476	0.002014150413731672
94-95	0.0	0.0	0.0	2.5352111257640555	0.002014150413731672
96-97	0.0	0.0	0.0	2.754375867658233	0.002039327293903318
98-99	0.0	0.0	0.0	2.992574330962175	0.0020645041740749636
100-101	0.0	0.0	0.0	3.267984223159809	0.0020645041740749636
102-103	0.0	0.0	0.0	3.57194469747209	0.002240742335276485
104-105	0.0	0.0	0.0	3.890860238606329	0.002316272975791423
106-107	0.0	0.0	0.0	4.216321768585195	0.002316272975791423
108-109	0.0	0.0	0.0	4.559935829167818	0.002316272975791423
110-111	0.0	0.0	0.0	4.936002888291693	0.0023918036163063606
112-113	0.0	0.0	0.0	5.341829019778453	0.0024169804964780063
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3090	0.0	80.26374	1
GTATCAA	6170	0.0	59.90886	1
TCAACGC	8195	0.0	44.534206	4
CAACGCA	8315	0.0	43.801136	5
ATCAACG	8585	0.0	42.513245	3
AACGCAG	8645	0.0	42.394886	6
TATCAAC	8830	0.0	41.604424	2
GTACATG	10515	0.0	36.74094	1
ACGCAGA	10005	0.0	36.450905	7
ACATGGG	11015	0.0	34.37769	3
TACATGG	11165	0.0	33.86336	2
CGCAGAG	10860	0.0	33.690716	8
CATGGGG	9155	0.0	32.12557	4
GCAGAGT	11480	0.0	30.989408	9
ATGGGGG	4950	0.0	29.094196	5
AGAGTAC	10710	0.0	27.134956	10-11
CAGAGTA	11230	0.0	26.673115	10-11
GTATAGG	560	0.0	25.550991	1
GAGTACT	6185	0.0	24.190306	12-13
AGTACTT	6480	0.0	23.593979	12-13
>>END_MODULE