FastQCFastQC Report
Mon 15 Aug 2016
SRR2921524_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921524_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040744
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.070.030821
GTATCAA40900.060.675831
TCAACGC50650.047.6893924
CAACGCA52150.046.6576545
AACGCAG55650.043.937016
ATCAACG56850.042.696693
TATCAAC59750.040.5248152
GTACATG97450.038.168151
ACGCAGA65400.037.0220037
TACATGG103850.035.3462372
ACATGGG103150.035.0093463
CGCAGAG70000.034.5857358
CATGGGG86950.032.4327624
GCAGAGT77850.030.4121029
AGAGTAC70900.025.9678710-11
ATGGGGG49250.025.2460775
CAGAGTA75700.025.0678310-11
ATGGGGA37800.023.7650665
GTCCTAA6500.021.0580161
CATGGGT18150.020.9786974
Produced by FastQC (version 0.11.5)