FastQCFastQC Report
Mon 15 Aug 2016
SRR2921524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040744
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32020.15690356066218986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.13519579133884504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.077.132821
GTATCAA58100.057.680341
TCAACGC76950.043.1007964
CAACGCA78300.042.4066855
AACGCAG81550.040.780636
TATCAAC83750.039.9596982
ATCAACG83450.039.8882983
GTACATG100450.036.6271251
TACATGG103650.034.9305152
ACGCAGA96600.034.3031127
ACATGGG105950.033.3845443
CGCAGAG101900.032.4013868
CATGGGG87900.030.5510674
GCAGAGT110850.029.6235779
AGAGTAC101650.027.06789410-11
GTGGTAT12000.025.8398341
CAGAGTA106400.025.83154910-11
ATGGGGG48100.025.361255
GAGTACT62550.024.25375412-13
AGTACTT64950.023.26594212-13
Produced by FastQC (version 0.11.5)