FastQCFastQC Report
Mon 15 Aug 2016
SRR2921523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512287
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.10864339903735203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.076.450111
GTATCAA35600.059.686571
TCAACGC45450.045.1602944
CAACGCA45950.044.5394135
ATCAACG47500.043.209843
AACGCAG47500.042.9579286
TATCAAC49600.042.100052
GTACATG79300.036.702371
ACGCAGA56550.036.188347
TACATGG82400.034.799822
ACATGGG82550.034.376253
CGCAGAG61200.033.2443288
CATGGGG69950.032.6599164
GCAGAGT66300.029.8785259
ATGGGGG39800.029.2984455
AGAGTAC59600.026.30129810-11
CAGAGTA63400.025.47553810-11
GAGTACT27500.021.30817812-13
ATGGGGA27900.020.4709785
TATACCG1754.7877498E-420.3978655
Produced by FastQC (version 0.11.5)