##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921523_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1512287
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.681888424617814	31.0	30.0	34.0	25.0	34.0
2	29.77120744937965	31.0	30.0	34.0	25.0	34.0
3	29.845619912093404	31.0	30.0	34.0	25.0	34.0
4	33.200117438025984	37.0	35.0	37.0	26.0	37.0
5	33.2320293700865	37.0	35.0	37.0	26.0	37.0
6	33.179998902324755	37.0	35.0	37.0	26.0	37.0
7	33.0698987692151	37.0	35.0	37.0	26.0	37.0
8	33.00956762836684	37.0	35.0	37.0	25.0	37.0
9	34.43680663789347	39.0	35.0	39.0	25.0	39.0
10-11	34.32982925859972	39.0	35.0	39.0	25.0	39.0
12-13	34.18199157964064	39.0	35.0	39.0	23.0	39.0
14-15	35.3151633916049	40.0	36.0	41.0	23.5	41.0
16-17	35.246770619597996	40.0	35.5	41.0	23.0	41.0
18-19	35.260718699559014	40.0	35.0	41.0	23.0	41.0
20-21	35.29255855535358	40.0	35.0	41.0	23.0	41.0
22-23	35.25860369096607	40.0	35.0	41.0	23.0	41.0
24-25	35.2196709354772	40.0	35.0	41.0	24.0	41.0
26-27	35.14611578357812	40.0	35.0	41.0	23.5	41.0
28-29	35.10236846577402	40.0	35.0	41.0	24.0	41.0
30-31	35.00599191820071	40.0	35.0	41.0	23.5	41.0
32-33	34.904867925202026	40.0	35.0	41.0	23.0	41.0
34-35	34.73723770686384	40.0	34.0	41.0	22.0	41.0
36-37	34.59821779860569	39.0	34.0	41.0	21.0	41.0
38-39	34.478638314023726	39.0	34.0	41.0	19.5	41.0
40-41	34.32879737774642	39.0	34.0	41.0	18.0	41.0
42-43	34.1364506208147	39.0	33.0	41.0	16.5	41.0
44-45	33.93979780293026	39.0	33.0	41.0	15.0	41.0
46-47	33.68818617101119	38.5	33.0	41.0	13.0	41.0
48-49	33.539398275591864	38.0	33.0	41.0	9.0	41.0
50-51	33.35235077733261	38.0	33.0	40.0	8.0	41.0
52-53	33.165965521094876	38.0	33.0	40.0	7.5	41.0
54-55	32.97468403814884	38.0	32.0	40.0	7.0	41.0
56-57	32.74689195899984	37.0	32.0	40.0	3.5	41.0
58-59	32.50872883255626	37.0	32.0	40.0	2.0	41.0
60-61	32.21435547617615	36.5	31.5	40.0	2.0	41.0
62-63	31.9031837210794	36.0	31.0	39.5	2.0	41.0
64-65	31.604397842473023	35.5	31.0	39.0	2.0	41.0
66-67	31.557961881574066	35.0	31.0	39.0	2.0	41.0
68-69	31.314768294642484	35.0	31.0	38.5	2.0	40.5
70-71	31.015613438454473	35.0	31.0	37.5	2.0	40.0
72-73	30.684342985160885	35.0	31.0	37.0	2.0	39.5
74-75	30.375084887987533	35.0	31.0	36.5	2.0	39.0
76-77	30.091236980811182	35.0	30.0	36.0	2.0	39.0
78-79	29.81149047766727	35.0	30.0	36.0	2.0	37.5
80-81	29.5636149090748	35.0	30.0	35.0	2.0	37.0
82-83	29.35631927008564	35.0	30.0	35.0	2.0	36.5
84-85	29.148762106663618	35.0	30.0	35.0	2.0	36.0
86-87	28.990445927261163	34.0	30.0	35.0	2.0	36.0
88-89	28.853132375005536	34.0	29.0	35.0	2.0	36.0
90-91	28.743478585744636	34.0	29.0	35.0	2.0	35.0
92-93	28.64082611303278	34.0	29.0	35.0	2.0	35.0
94-95	28.57796998850086	34.0	29.0	35.0	2.0	35.0
96-97	28.47228998199416	34.0	29.0	35.0	2.0	35.0
98-99	28.40871375605292	34.0	29.0	35.0	2.0	35.0
100-101	28.34188748564261	34.0	29.0	35.0	2.0	35.0
102-103	27.38070187735529	32.5	26.5	34.5	2.0	35.0
104-105	28.18641798811998	34.0	29.0	35.0	2.0	35.0
106-107	28.49897274789772	34.0	29.5	35.0	2.0	35.0
108-109	28.538087677801897	35.0	30.0	35.0	2.0	35.0
110-111	28.48001007745223	35.0	30.0	35.0	2.0	35.0
112-113	28.409263585549567	35.0	29.0	35.0	2.0	35.0
114-115	28.29376897374639	35.0	29.0	35.0	2.0	35.0
116-117	28.232661855851433	34.5	29.0	35.0	2.0	35.0
118-119	28.06926694470031	34.0	29.0	35.0	2.0	35.0
120-121	27.89630440518235	34.0	29.0	35.0	2.0	35.0
122-123	27.776193606107835	34.0	29.0	35.0	2.0	35.0
124-125	26.95106186854744	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66081.0
3	11104.0
4	7043.0
5	6004.0
6	6317.0
7	7170.0
8	7876.0
9	8393.0
10	8722.0
11	9012.0
12	9240.0
13	10009.0
14	11317.0
15	9901.0
16	7271.0
17	6190.0
18	5571.0
19	5332.0
20	5014.0
21	5289.0
22	6074.0
23	8191.0
24	11571.0
25	16106.0
26	19521.0
27	20619.0
28	23759.0
29	28907.0
30	32194.0
31	37910.0
32	47635.0
33	61446.0
34	86100.0
35	132749.0
36	253732.0
37	394637.0
38	118103.0
39	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15910747619659	16.94947207158818	12.10359065405971	24.787829798155524
2	16.69085228167136	19.13040257474281	38.72551289157216	25.45323225201367
3	19.22215306792635	24.30741899191556	28.594162245678827	27.87626569447926
4	13.249219494126363	15.027119271880624	35.4796803894592	36.243980844533816
5	14.269877114512763	37.273434430425965	33.47329764111194	14.983390813949335
6	33.71980868081052	34.73521609409835	17.084366999424454	14.460608225666673
7	29.773097564541278	31.267236309681707	20.567080790126656	18.39258533565036
8	27.758113518816064	34.00775384037355	19.25892019074368	18.97521245006671
9	27.780195706728293	14.720667471859056	18.665029824308434	38.834106997104215
10-11	26.357511109464777	25.05377787949786	26.870352314565537	21.718358696471824
12-13	26.51301700487342	22.738573429533876	27.106393323069444	23.642016242523265
14-15	23.983472697969937	23.830674860423144	24.40551884622419	27.78033359538273
16-17	24.048018378645473	26.89044418257065	24.920712986136305	24.140824452647568
18-19	24.15549910903217	26.073651797135213	26.57684105510968	23.194008038722945
20-21	25.225515506548117	25.059777984499405	26.238763922282665	23.475942586669806
22-23	24.97301531486323	24.706691441083077	26.122994590492727	24.19729865356097
24-25	24.403286571979447	25.095939953498895	26.258996851846216	24.24177662267544
26-27	24.142621250291086	25.17620588733408	26.3052202193196	24.375952643055232
28-29	24.19853782231981	25.32166652002109	26.302262333278865	24.177533324380235
30-31	24.515930138209278	25.239376296859355	26.10257646859967	24.1421170963317
32-33	24.181720084010458	25.20717297665829	26.178539754286945	24.432567185044313
34-35	24.382821399229222	25.264268864960265	26.27391484390607	24.078994891904443
36-37	24.481148817775818	25.2001192164397	26.09457330339029	24.224158662394192
38-39	24.500184926780243	25.368976895069434	25.989656009723376	24.141182168426944
40-41	24.4650922785076	25.217444516296556	26.200646007439175	24.11681719775667
42-43	24.31819723080944	25.2215997525559	26.07971471151095	24.380488305123713
44-45	24.28422139424532	25.319519652836338	26.052161221503745	24.3440977314146
46-47	24.451845399510972	25.33190989743388	25.904008061578708	24.31223664147644
48-49	24.436143324535674	25.507607454865834	25.70134558191398	24.354903638684515
50-51	24.397165419654637	25.639237694639235	25.558459498268824	24.405137387437303
52-53	24.502396588108066	25.593622419189376	25.6102624056392	24.293718587063353
54-55	24.43363405003007	25.437496816310546	25.736945550144846	24.39192358351454
56-57	24.344745077298267	25.555380249787568	25.59950438082499	24.50037029208918
58-59	24.400700167169543	25.53731413386397	25.608395919925325	24.45358977904116
60-61	24.534625133616263	25.470302792976813	25.75526262872141	24.239809444685513
62-63	24.34820804552877	25.623792924266343	25.810243642830944	24.217755387373945
64-65	24.392667322232377	25.270139709586374	25.849299930573537	24.48789303760771
66-67	24.307459366352234	25.56925803222116	25.703372304195643	24.419910297230963
68-69	24.337117015457295	25.49484304917905	25.76229806931011	24.40574186605355
70-71	24.303456470122807	25.403934052717336	25.81575724858442	24.476852228575435
72-73	24.43501362753778	25.61910957492864	25.56328313694311	24.38259366059047
74-75	24.34418985551538	25.708366242910387	25.62111452003614	24.32632938153809
76-77	24.37608643311276	25.378449135748248	25.746447347184997	24.499017083953994
78-79	24.301841064600808	25.444143151065724	25.761807575064548	24.492208209268924
80-81	24.351580582786266	25.692750275889736	25.516022108217562	24.43964703310644
82-83	24.464104606701408	25.622980564811904	25.550098196095238	24.36281663239145
84-85	24.291780463600357	25.668884570144378	25.460718439352558	24.578616526902703
86-87	24.16165684543296	25.862449819117344	25.562058953856738	24.41383438159296
88-89	24.27961764798283	25.57019625220947	25.624959696287757	24.52522640351994
90-91	24.251755993458136	25.5477952287768	25.626890160550808	24.573558617214257
92-93	24.2816265373994	25.754732361139265	25.591538942050295	24.372102159411035
94-95	24.3715360204275	25.49522032406365	25.59690148566924	24.536342169839614
96-97	24.26779390996316	25.570992619329548	25.619864224232714	24.54134924647458
98-99	24.19498781689868	25.590431545321167	25.537923023432757	24.67665761434739
100-101	24.138063298024477	25.610024253826964	25.622490092634077	24.62942235551448
102-103	24.157656863811592	25.529635553074982	25.70669599268084	24.606011590432587
104-105	24.116328566435843	25.664801502711775	25.63107579390153	24.587794136950848
106-107	24.048458123560412	25.646578946150218	25.672601479614137	24.63236145067523
108-109	24.045552392194985	25.621203553986	25.658370285132314	24.674873768686698
110-111	24.091942955901622	25.757200526253666	25.585728328655264	24.565128189189448
112-113	24.000425878324446	25.760778061182315	25.6830751060232	24.555720954470036
114-115	24.016286577097546	25.907316361866478	25.592965849306836	24.48343121172914
116-117	23.852393184803788	25.785728479985348	25.69112992207605	24.670748413134817
118-119	24.11971340801307	25.59443707134173	25.63016826142238	24.655681259222824
120-121	23.870602922524625	25.69205375151572	25.756233278976527	24.681110046983132
122-123	23.802203806093107	25.83621668709433	25.68153751821457	24.680041988597992
124-125	23.866610590689007	25.796173079034062	25.726970825943603	24.610245504333324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	149.0
1	166.5
2	206.0
3	213.5
4	184.5
5	168.5
6	168.5
7	216.5
8	317.5
9	372.5
10	404.5
11	464.5
12	527.0
13	632.0
14	717.0
15	788.5
16	858.5
17	940.0
18	1069.0
19	1177.5
20	1293.0
21	1485.0
22	1746.0
23	2174.5
24	2650.0
25	3250.5
26	4152.5
27	5190.0
28	6112.0
29	7321.0
30	9154.5
31	10942.5
32	12965.0
33	15224.0
34	17637.5
35	20263.0
36	23395.0
37	26461.0
38	28713.5
39	31323.5
40	33769.5
41	35754.5
42	38079.0
43	41955.0
44	46308.5
45	50099.5
46	52780.5
47	55101.0
48	58868.0
49	62064.5
50	62477.0
51	62308.0
52	62121.5
53	61678.0
54	62508.0
55	61694.5
56	58174.5
57	53583.5
58	50197.5
59	47001.0
60	41597.0
61	35828.5
62	30292.5
63	23783.5
64	18011.0
65	14190.0
66	11134.5
67	8702.0
68	6922.0
69	5512.0
70	4436.0
71	3491.0
72	2696.5
73	2242.0
74	1816.0
75	1368.5
76	970.0
77	589.0
78	347.5
79	232.5
80	136.0
81	59.5
82	40.5
83	33.5
84	28.0
85	20.0
86	17.5
87	22.5
88	18.0
89	10.0
90	11.0
91	7.5
92	6.0
93	4.5
94	3.5
95	6.5
96	6.5
97	5.0
98	5.5
99	8.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03498013273935437
2	0.02440013039852885
3	0.02446625541315901
4	0.02955788153968129
5	0.03127713192006544
6	0.04476663490461797
7	0.0383525084854925
8	0.034385007607682934
9	0.02863213133485906
10-11	0.03392213250527182
12-13	0.034021320027217056
14-15	0.032103694602942434
16-17	0.03521157029055992
18-19	0.048172073158071184
20-21	0.05693363759656731
22-23	0.05167669893346964
24-25	0.061165638532897526
26-27	0.04648588528500212
28-29	0.0466842603288926
30-31	0.046452822777687044
32-33	0.05346207432848395
34-35	0.037625133324560744
36-37	0.0496268234799347
38-39	0.05805776284528003
40-41	0.04592382266064576
42-43	0.05518132470886809
44-45	0.055445824767388734
46-47	0.060868075967061806
48-49	0.04698182289472832
50-51	0.0489655733336331
52-53	0.057363450191663355
54-55	0.04470050988998781
56-57	0.04248532189987747
58-59	0.04284900948034334
60-61	0.032731882241928946
62-63	0.04149344668042508
64-65	0.040699946504863166
66-67	0.03415357005647737
68-69	0.029359506495790808
70-71	0.0473455104751942
72-73	0.030318319207928126
74-75	0.037625133324560744
76-77	0.0303513817152432
78-79	0.0311779443981202
80-81	0.023838067774172494
82-83	0.017291691325786708
84-85	0.01808519150134862
86-87	0.016994128759950988
88-89	0.02178819232063755
90-91	0.011902502633428708
92-93	0.018349691559869256
94-95	0.013720940535758092
96-97	0.010679189862770756
98-99	0.010017939716469164
100-101	0.01021631476035964
102-103	0.006050438838659593
104-105	0.005785938780138955
106-107	0.008993001989701689
108-109	0.012034752662689026
110-111	0.0058520637947691145
112-113	0.00780275172635882
114-115	0.008034189277564377
116-117	0.007108439072742144
118-119	0.2513411806092362
120-121	0.36871308157776933
122-123	0.029624006554311452
124-125	0.003504625775398453
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1512287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.78804930016676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.78026832765683	51.348235195377825
2	9.955544749005817	12.50178469021187
3	3.139525028845995	5.913739568708694
4	1.532801654883099	3.8496650349670882
5	0.8805009889078359	2.7642469750195393
6	0.5780764898528276	2.1777777086488004
7	0.40965117788070743	1.8004838846851667
8	0.2811033299345492	1.4119943790697211
9	0.2144165920423012	1.2116519596733175
>10	1.152448100890553	12.682552382591133
>50	0.059027370723627126	2.4834647179955165
>100	0.016318321140565903	1.6104462262795878
>500	1.0595607845720417E-4	0.04929706629067044
>1k	2.1191215691440833E-4	0.1946602104811089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1643	0.10864339903735203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.612501463015949E-5	6.612501463015949E-5
2	0.0	0.0	0.0	6.612501463015949E-5	6.612501463015949E-5
3	0.0	0.0	0.0	1.3225002926031898E-4	6.612501463015949E-5
4	0.0	0.0	0.0	1.3225002926031898E-4	6.612501463015949E-5
5	0.0	0.0	0.0	1.3225002926031898E-4	6.612501463015949E-5
6	0.0	0.0	0.0	1.3225002926031898E-4	6.612501463015949E-5
7	0.0	0.0	0.0	1.3225002926031898E-4	6.612501463015949E-5
8	0.0	0.0	0.0	1.9837504389047845E-4	6.612501463015949E-5
9	0.0	0.0	0.0	1.9837504389047845E-4	6.612501463015949E-5
10-11	0.0	0.0	0.0	1.9837504389047845E-4	6.612501463015949E-5
12-13	0.0	0.0	0.0	1.9837504389047845E-4	1.9837504389047845E-4
14-15	0.0	0.0	0.0	1.9837504389047845E-4	1.9837504389047845E-4
16-17	0.0	0.0	0.0	2.6450005852063797E-4	1.9837504389047845E-4
18-19	0.0	0.0	0.0	2.6450005852063797E-4	2.6450005852063797E-4
20-21	0.0	0.0	0.0	4.628751024111164E-4	2.6450005852063797E-4
22-23	0.0	0.0	0.0	4.628751024111164E-4	3.3062507315079746E-4
24-25	0.0	0.0	0.0	5.290001170412759E-4	3.3062507315079746E-4
26-27	0.0	0.0	0.0	5.620626243563557E-4	3.636875804658772E-4
28-29	0.0	0.0	0.0	0.0013555627999182695	3.967500877809569E-4
30-31	0.0	0.0	0.0	0.0035376882827135325	4.2981259509603664E-4
32-33	0.0	0.0	0.0	0.0078358142336739	4.628751024111164E-4
34-35	0.0	0.0	0.0	0.014845065784470805	4.628751024111164E-4
36-37	0.0	0.0	0.0	0.024763817978994726	4.628751024111164E-4
38-39	0.0	0.0	0.0	0.040104821373191726	5.290001170412759E-4
40-41	0.0	0.0	0.0	0.056040949899060166	5.620626243563557E-4
42-43	0.0	0.0	0.0	0.07260526606391512	6.281876389865152E-4
44-45	0.0	0.0	0.0	0.09197989535055184	6.612501463015949E-4
46-47	0.0	0.0	0.0	0.11499140044184736	6.612501463015949E-4
48-49	0.0	0.0	0.0	0.13849884314286903	7.273751609317544E-4
50-51	0.0	0.0	0.0	0.16597378672170032	7.935001755619138E-4
52-53	0.0	0.0	0.0	0.19235766755913394	8.265626828769935E-4
54-55	0.0	0.0	0.0	0.22227923667928112	8.596251901920733E-4
56-57	0.0	0.0	0.0	0.25620136918455294	8.92687697507153E-4
58-59	0.0	0.0	0.0	0.2973641907918272	9.257502048222328E-4
60-61	0.0	0.0	0.0	0.3379649497747451	9.257502048222328E-4
62-63	0.0	0.0	0.0	0.38412020998659646	9.918752194523924E-4
64-65	0.0	0.0	0.0	0.42915134494973506	9.918752194523924E-4
66-67	0.0	0.0	0.0	0.4795716686052317	9.918752194523924E-4
68-69	0.0	0.0	0.0	0.5410018071966498	9.918752194523924E-4
70-71	0.0	0.0	0.0	0.6106645101095229	0.0010580002340825519
72-73	0.0	0.0	0.0	0.6814182757637934	0.0010580002340825519
74-75	0.0	0.0	0.0	0.7519736663741736	0.0010580002340825519
76-77	0.0	0.0	0.0	0.8365806225934628	0.0010580002340825519
78-79	0.0	0.0	0.0	0.9302136433097685	0.0010580002340825519
80-81	0.0	0.0	0.0	1.029830977850104	0.0010580002340825519
82-83	0.0	0.0	0.0	1.14025975228247	0.0011241252487127114
84-85	0.0	0.0	0.0	1.2514489643830835	0.0011241252487127114
86-87	0.0	0.0	0.0	1.3721932410977546	0.0011241252487127114
88-89	0.0	0.0	0.0	1.5061625207384577	0.0011241252487127114
90-91	0.0	0.0	0.0	1.6619861177144286	0.0011241252487127114
92-93	0.0	0.0	0.0	1.8224384657145105	0.0011241252487127114
94-95	0.0	0.0	0.0	1.9915862531384585	0.0011241252487127114
96-97	0.0	0.0	0.0	2.168073917186354	0.0011902502633428708
98-99	0.0	0.0	0.0	2.36545708585738	0.0012563752779730303
100-101	0.0	0.0	0.0	2.5969607620775683	0.0012563752779730303
102-103	0.0	0.0	0.0	2.839209753175158	0.0013555627999182695
104-105	0.0	0.0	0.0	3.0969981227108345	0.0013886253072333493
106-107	0.0	0.0	0.0	3.3615973687534177	0.0013886253072333493
108-109	0.0	0.0	0.0	3.6339663040150447	0.0013886253072333493
110-111	0.0	0.0	0.0	3.937711558718682	0.0014547503218635088
112-113	0.0	0.0	0.0	4.271113882483947	0.0014547503218635088
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1705	0.0	76.45011	1
GTATCAA	3560	0.0	59.68657	1
TCAACGC	4545	0.0	45.160294	4
CAACGCA	4595	0.0	44.539413	5
ATCAACG	4750	0.0	43.20984	3
AACGCAG	4750	0.0	42.957928	6
TATCAAC	4960	0.0	42.10005	2
GTACATG	7930	0.0	36.70237	1
ACGCAGA	5655	0.0	36.18834	7
TACATGG	8240	0.0	34.79982	2
ACATGGG	8255	0.0	34.37625	3
CGCAGAG	6120	0.0	33.244328	8
CATGGGG	6995	0.0	32.659916	4
GCAGAGT	6630	0.0	29.878525	9
ATGGGGG	3980	0.0	29.298445	5
AGAGTAC	5960	0.0	26.301298	10-11
CAGAGTA	6340	0.0	25.475538	10-11
GAGTACT	2750	0.0	21.308178	12-13
ATGGGGA	2790	0.0	20.470978	5
TATACCG	175	4.7877498E-4	20.397865	5
>>END_MODULE