FastQCFastQC Report
Mon 15 Aug 2016
SRR2921523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512287
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.19500266814434034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26270.17371041343342897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.083.5074161
GTATCAA46200.058.3211561
TCAACGC61350.043.0960854
CAACGCA62300.042.3167275
ATCAACG63900.041.5626683
TATCAAC65150.040.8579832
AACGCAG65400.040.4821666
GTACATG77700.036.8256031
ACGCAGA75300.034.9216237
TACATGG81250.034.520842
ACATGGG82450.033.656173
CGCAGAG79900.032.9100278
CATGGGG69050.031.3049034
GCAGAGT88250.029.0536699
ATGGGGG37250.028.7569375
CAGAGTA85250.026.44785110-11
AGAGTAC81650.026.41176410-11
GAGTACT50250.025.21716712-13
CGTATAC1451.3329847E-424.6406783
GTACTTT52200.024.21816314-15
Produced by FastQC (version 0.11.5)