##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1512287
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192434372576106	31.0	31.0	34.0	28.0	34.0
2	31.264342019735672	33.0	31.0	34.0	28.0	34.0
3	31.35132749273121	33.0	31.0	34.0	28.0	34.0
4	34.86372692484958	37.0	35.0	37.0	32.0	37.0
5	34.5151846177346	37.0	35.0	37.0	30.0	37.0
6	34.48466924598307	37.0	35.0	37.0	30.0	37.0
7	34.33286406614617	37.0	35.0	37.0	30.0	37.0
8	34.30137864043002	37.0	35.0	37.0	30.0	37.0
9	35.86540782272148	39.0	37.0	39.0	30.0	39.0
10-11	35.73879462033331	39.0	36.0	39.0	30.0	39.0
12-13	35.63336258263147	39.0	35.0	39.0	30.0	39.0
14-15	36.80503270873849	40.0	37.0	41.0	30.0	41.0
16-17	36.7901410248187	40.0	37.0	41.0	30.0	41.0
18-19	36.814316991417634	40.0	37.0	41.0	30.0	41.0
20-21	36.80623651462983	40.0	37.0	41.0	30.0	41.0
22-23	36.7506409167043	40.0	37.0	41.0	30.0	41.0
24-25	36.68168641269811	40.0	36.5	41.0	30.0	41.0
26-27	36.5626015432256	40.0	36.0	41.0	29.5	41.0
28-29	36.37544394681697	40.0	36.0	41.0	28.5	41.0
30-31	36.33023890306536	40.0	36.0	41.0	29.0	41.0
32-33	36.20478520280872	40.0	35.5	41.0	29.0	41.0
34-35	36.08279347769306	40.0	35.0	41.0	28.0	41.0
36-37	35.95266639202744	40.0	35.0	41.0	28.0	41.0
38-39	35.7883037412872	40.0	35.0	41.0	27.0	41.0
40-41	35.59047885751845	40.0	35.0	41.0	26.5	41.0
42-43	35.418360073187166	39.0	35.0	41.0	26.0	41.0
44-45	35.20293932302532	39.0	35.0	41.0	25.0	41.0
46-47	34.924188662601736	39.0	34.5	41.0	24.0	41.0
48-49	34.66019545231825	39.0	34.0	41.0	23.0	41.0
50-51	34.44722496457352	39.0	34.0	41.0	22.0	41.0
52-53	34.247965829237444	38.5	34.0	41.0	22.0	41.0
54-55	34.035128583397196	38.0	33.5	40.0	21.0	41.0
56-57	33.78498062867696	38.0	33.0	40.0	20.0	41.0
58-59	33.63926853831316	37.0	33.0	40.0	20.0	41.0
60-61	33.52774341113823	37.0	33.0	40.0	20.0	41.0
62-63	33.25703487499396	36.5	33.0	40.0	19.5	41.0
64-65	32.96260498172635	36.0	33.0	39.5	18.0	41.0
66-67	32.66456796891066	35.5	33.0	39.0	18.0	41.0
68-69	32.344487521217864	35.0	32.0	39.0	17.0	41.0
70-71	32.040710857132275	35.0	32.0	37.5	15.5	40.0
72-73	31.72191918597462	35.0	32.0	37.0	13.0	39.5
74-75	31.42574425357092	35.0	32.0	37.0	12.0	39.0
76-77	31.127472166328218	35.0	32.0	36.0	9.0	39.0
78-79	30.84561693646775	35.0	31.0	36.0	7.5	37.5
80-81	30.61655195078712	35.0	31.0	35.0	7.0	37.0
82-83	30.423877213782834	35.0	31.0	35.0	7.0	37.0
84-85	30.223768372008752	35.0	31.0	35.0	6.5	36.0
86-87	30.072247529734767	35.0	31.0	35.0	3.5	36.0
88-89	29.96032532184698	35.0	31.0	35.0	2.0	36.0
90-91	29.861199957415494	35.0	31.0	35.0	2.0	35.0
92-93	29.91112302096097	35.0	31.0	35.0	2.0	35.0
94-95	29.87470268540297	35.0	31.0	35.0	2.0	35.0
96-97	29.850502252548623	35.0	31.0	35.0	2.0	35.0
98-99	29.811174069472266	35.0	31.0	35.0	2.0	35.0
100-101	29.758693951611036	35.0	31.0	35.0	2.0	35.0
102-103	28.88582557411391	33.5	29.5	35.0	2.0	35.0
104-105	29.585744967721077	34.5	31.0	35.0	2.0	35.0
106-107	29.691372074216073	35.0	31.0	35.0	2.0	35.0
108-109	29.695599446401374	35.0	31.0	35.0	2.0	35.0
110-111	29.663727189349643	35.0	31.0	35.0	2.0	35.0
112-113	29.620323721621624	35.0	31.0	35.0	2.0	35.0
114-115	29.55442584641672	35.0	31.0	35.0	2.0	35.0
116-117	29.47856193963183	35.0	31.0	35.0	2.0	35.0
118-119	29.38645839050392	35.0	31.0	35.0	2.0	35.0
120-121	29.265483668113262	35.0	31.0	35.0	2.0	35.0
122-123	29.15026612012138	35.0	31.0	35.0	2.0	35.0
124-125	28.24479182853519	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26219.0
3	9587.0
4	5287.0
5	4284.0
6	4705.0
7	5403.0
8	6354.0
9	7010.0
10	8000.0
11	8779.0
12	9258.0
13	10726.0
14	13733.0
15	11906.0
16	6643.0
17	5639.0
18	4973.0
19	4587.0
20	4465.0
21	4613.0
22	5474.0
23	7238.0
24	10017.0
25	14100.0
26	17402.0
27	19406.0
28	22770.0
29	27163.0
30	31663.0
31	37338.0
32	46843.0
33	60230.0
34	83042.0
35	129620.0
36	259807.0
37	433555.0
38	144067.0
39	381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.045627801874105	17.57678401295574	12.484068504932225	24.893519680237926
2	16.930122881577287	19.570724520571407	37.589608727018806	25.9095438708325
3	19.150260314705665	24.368194516460143	28.97442884202284	27.507116326811353
4	13.365644188016091	15.538256934152022	35.118039381748886	35.978059496083
5	14.17591248358902	37.25896113945944	33.3775368813019	15.187589495649641
6	33.92334890217818	34.99749337629647	16.413358783169773	14.665798938355575
7	29.446921193098436	31.66587635568095	20.345184030030495	18.542018421190125
8	27.82312034450652	34.06458968173354	18.80032429744371	19.311965676316234
9	27.735904204668095	14.352966227225597	18.549037708699238	39.36209185940707
10-11	26.452799394782645	24.9744409410495	26.613890902165345	21.958868762002506
12-13	26.760410167493102	22.673977813288683	27.187929730728804	23.377682288489407
14-15	24.12603955921003	23.601188832853527	24.729217825615585	27.543553782320856
16-17	23.906123368075637	26.660311407689864	25.23573480780632	24.19783041642818
18-19	23.94023397745135	25.45541354730898	27.435248185256285	23.16910428998338
20-21	25.009101535439775	24.40315916692431	27.280104093128298	23.307635204507616
22-23	24.726422758716694	24.140333427790985	27.176391891896362	23.956851921595963
24-25	24.16776311112243	24.629575926477653	27.28273452971709	23.919926432682832
26-27	23.92450639137734	24.697840068590875	27.340327988972025	24.037325551059762
28-29	24.01917618967471	24.823827520107187	27.295946288680483	23.861050001537613
30-31	24.28775607117016	24.721888223537054	27.21004615134307	23.780309553949717
32-33	23.92748861098867	24.712757511357598	27.228210424818304	24.131543452835423
34-35	24.099512760361037	24.811721264799232	27.234757970802658	23.854008004037073
36-37	24.277040725391377	24.769347115355046	27.018355343498452	23.935256815755128
38-39	24.313155015985597	24.908894659077532	26.885665608014893	23.89228471692198
40-41	24.238493413943473	24.77776440447738	27.0175460640393	23.966196117539848
42-43	24.189061619115797	24.81641236581628	26.82609723556321	24.168428779504715
44-45	24.15882765122518	24.92719280522393	26.704723382261662	24.209256161289225
46-47	24.41701200631201	24.99530435081658	26.37040206661473	24.21728157625668
48-49	24.43532689894913	25.246282856729017	25.954922713975904	24.36346753034595
50-51	24.349169481795794	25.395286455371053	25.863420163643436	24.39212389918972
52-53	24.546559367182162	25.441816705716224	25.815105152723188	24.19651877437842
54-55	24.382577755438458	25.299919148563276	25.92788184116413	24.38962125483414
56-57	24.3333932986152	25.446234290529148	25.820014206480746	24.400358204374907
58-59	24.330416856944733	25.303968178122094	25.979015787595777	24.386599177337395
60-61	24.487753742164724	25.322182484370785	26.029328849184107	24.160734924280376
62-63	24.363862677805283	25.371979864773074	26.082208922490224	24.18194853493142
64-65	24.332014022241015	25.107000439476423	26.087869491705334	24.473116046577225
66-67	24.363331912581785	25.436699235935468	25.919501253380623	24.280467598102124
68-69	24.35223907842843	25.403207404531532	25.854019242839826	24.390534274200213
70-71	24.28491169546882	25.14843077545759	26.11482217902165	24.45183535005193
72-73	24.497010152222156	25.4087293700834	25.75121516818245	24.34304530951199
74-75	24.234782453418305	25.578646063778216	25.761976850850616	24.42459463195286
76-77	24.341905992616862	25.30990136856571	25.83172739260919	24.516465246208238
78-79	24.319304536250016	25.280144352515403	25.84395522188147	24.556595889353108
80-81	24.332283276046457	25.512952874407812	25.659581701092804	24.495182148452923
82-83	24.44136715137339	25.462528060184447	25.572272354985593	24.52383243345657
84-85	24.308884026634455	25.52904021323965	25.5025208294549	24.65955493067099
86-87	24.186317502663503	25.613729716217172	25.641637794150814	24.558314986968515
88-89	24.238616281653773	25.37265605985248	25.63912713362046	24.74960052487328
90-91	24.195953712117305	25.39061518336726	25.666621696155417	24.74680940836002
92-93	24.210735844165463	25.62479830283408	25.6346186402569	24.529847212743558
94-95	24.251662329675273	25.33479699904443	25.68376223808595	24.72977843319435
96-97	24.156501124973754	25.458098240544352	25.68345668644394	24.70194394803795
98-99	24.068769897778854	25.473653514060572	25.568974392969995	24.888602195190575
100-101	24.062033149332034	25.45811297596035	25.651648307318105	24.82820556738951
102-103	24.035073797809872	25.29287679204359	25.764627307834736	24.907422102311802
104-105	24.053381350053662	25.52079648153646	25.59674286874335	24.829079299666525
106-107	24.039886727512883	25.371350921537477	25.676551834726645	24.912210516222995
108-109	24.061416179236673	25.320863855577862	25.638751544299915	24.978968420885547
110-111	24.05499258898753	25.48806385604749	25.543311689363684	24.913631865601296
112-113	23.89239198362602	25.44325813160688	25.64799904771667	25.01635083705043
114-115	23.834968308916153	25.600808064777436	25.599187967637732	24.965035658668675
116-117	23.755547487684925	25.452974564384583	25.604412463934306	25.18706548399619
118-119	23.8186325923555	25.286017355467	25.678175255563946	25.217174796613556
120-121	23.618468277383233	25.395683381876577	25.8518984106537	25.133949930086487
122-123	23.629453540349672	25.46515856851906	25.644556563599334	25.26083132753194
124-125	23.675479715084528	25.360823924010194	25.68525293226279	25.278443428642483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	55.5
2	81.5
3	124.5
4	109.0
5	83.0
6	150.5
7	339.0
8	584.5
9	677.0
10	599.0
11	530.5
12	576.5
13	691.0
14	821.0
15	984.0
16	1129.5
17	1273.5
18	1451.5
19	1616.0
20	1789.0
21	1982.5
22	2325.5
23	2813.5
24	3178.0
25	3707.5
26	4619.0
27	5657.0
28	6508.0
29	7635.5
30	9333.0
31	10945.0
32	12975.0
33	15201.5
34	17444.5
35	19837.0
36	22892.0
37	25869.0
38	27902.5
39	30402.0
40	32830.0
41	34959.5
42	37239.5
43	41110.5
44	45506.0
45	49232.5
46	52363.0
47	55109.5
48	59196.5
49	62323.5
50	62617.0
51	62464.5
52	62126.5
53	62043.0
54	63136.5
55	62143.0
56	58604.5
57	54314.0
58	50776.0
59	47178.0
60	41609.5
61	36065.5
62	30382.5
63	23683.5
64	17967.0
65	13784.0
66	10708.0
67	8476.5
68	6690.5
69	5287.0
70	4274.5
71	3375.5
72	2615.0
73	2192.5
74	1739.5
75	1273.0
76	869.5
77	499.5
78	290.0
79	144.5
80	74.0
81	39.0
82	18.0
83	13.0
84	10.5
85	5.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12656327800212525
2	0.0011902502633428708
3	0.01791987896477322
4	0.06129788856215785
5	0.022614755003514543
6	0.01937462928663673
7	0.014812003277155726
8	0.006281876389865151
9	0.004165875921700048
10-11	0.006810876506906427
12-13	0.004992438604577041
14-15	0.0031409381949325755
16-17	0.010150189745729482
18-19	0.005951251316714354
20-21	0.011869440126113628
22-23	0.009621189628688204
24-25	0.013390315462607296
26-27	0.008761564438496131
28-29	0.013456440477237454
30-31	0.020168129462198644
32-33	0.012795190330935862
34-35	0.019837504389047845
36-37	0.014580565725950166
38-39	0.019903629403678007
40-41	0.010117127238414401
42-43	0.00889381446775645
44-45	0.016233691091704153
46-47	0.016465128642909714
48-49	0.020234254476828802
50-51	0.014481378204004927
52-53	0.021126942174335958
54-55	0.01682881622337559
56-57	0.019903629403678007
58-59	0.016233691091704153
60-61	0.01947381680858197
62-63	0.020498754535349443
64-65	0.017225566311156546
66-67	0.01167106508222315
68-69	0.0230115050912955
70-71	0.014448315696689848
72-73	0.01676269120874543
74-75	0.017589253891622424
76-77	0.01276212782362078
78-79	0.02859906882754398
80-81	0.020234254476828802
82-83	0.00895993948238661
84-85	0.012266190213894586
86-87	0.011902502633428708
88-89	0.020035879432938327
90-91	0.012233127706579505
92-93	0.007868876740988979
94-95	0.006744751492276268
96-97	0.001950687931589705
98-99	0.0017523128876992264
100-101	0.010580002340825517
102-103	0.003107875687617496
104-105	0.0023805005266857417
106-107	2.975625658357177E-4
108-109	0.01818437902329386
110-111	6.943126536166747E-4
112-113	0.008728501931181052
114-115	0.0021160004681651037
116-117	0.007670501697098501
118-119	0.009323627062852488
120-121	0.004066688399754808
122-123	4.6287510241111644E-4
124-125	0.026450005852063797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1512287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.06964461943893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84384190983856	49.371046951039446
2	10.331432311931366	12.618737993988738
3	3.3720160841715723	6.1778347173417
4	1.6519122228364713	4.035267695645228
5	0.9683737778047007	2.95691212346583
6	0.6062659007148665	2.2214665860924567
7	0.4141080095519059	1.7702600284178682
8	0.3006443977367176	1.4688197221285277
9	0.21268807735373096	1.1689906768905678
>10	1.214486579759381	13.069549287553924
>50	0.06320148381898903	2.6026133987052105
>100	0.020595680363304135	2.0345657705228146
>500	1.083910296030429E-4	0.037201037316796186
>1k	3.251730888091287E-4	0.4667340108909785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2949	0.19500266814434034	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2627	0.17371041343342897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.612501463015949E-5
2	0.0	0.0	0.0	0.0	6.612501463015949E-5
3	0.0	0.0	0.0	0.0	6.612501463015949E-5
4	0.0	0.0	0.0	0.0	6.612501463015949E-5
5	0.0	0.0	0.0	0.0	6.612501463015949E-5
6	0.0	0.0	0.0	0.0	6.612501463015949E-5
7	0.0	0.0	0.0	0.0	6.612501463015949E-5
8	0.0	0.0	0.0	0.0	6.612501463015949E-5
9	0.0	0.0	0.0	0.0	6.612501463015949E-5
10-11	0.0	0.0	0.0	3.3062507315079746E-5	6.612501463015949E-5
12-13	0.0	0.0	0.0	9.918752194523924E-5	1.3225002926031898E-4
14-15	0.0	0.0	0.0	1.3225002926031898E-4	1.3225002926031898E-4
16-17	0.0	0.0	0.0	1.9837504389047845E-4	1.3225002926031898E-4
18-19	0.0	0.0	0.0	1.9837504389047845E-4	1.6531253657539873E-4
20-21	0.0	0.0	0.0	1.9837504389047845E-4	2.3143755120555822E-4
22-23	0.0	0.0	0.0	1.9837504389047845E-4	2.6450005852063797E-4
24-25	0.0	0.0	0.0	2.6450005852063797E-4	2.6450005852063797E-4
26-27	0.0	0.0	0.0	2.975625658357177E-4	3.3062507315079746E-4
28-29	0.0	0.0	0.0	0.001157187756027791	3.3062507315079746E-4
30-31	0.0	0.0	0.0	0.0034715632680833728	3.3062507315079746E-4
32-33	0.0	0.0	0.0	0.008067251784879458	3.3062507315079746E-4
34-35	0.0	0.0	0.0	0.015241815872251763	3.3062507315079746E-4
36-37	0.0	0.0	0.0	0.025127505559460604	3.3062507315079746E-4
38-39	0.0	0.0	0.0	0.0409644465633838	3.9675008778095695E-4
40-41	0.0	0.0	0.0	0.05752876272823876	4.628751024111164E-4
42-43	0.0	0.0	0.0	0.07439064145892943	4.959376097261962E-4
44-45	0.0	0.0	0.0	0.09492245850159395	5.620626243563557E-4
46-47	0.0	0.0	0.0	0.11842990120261565	5.951251316714354E-4
48-49	0.0	0.0	0.0	0.1421687814548429	5.951251316714354E-4
50-51	0.0	0.0	0.0	0.17083397529701705	6.612501463015949E-4
52-53	0.0	0.0	0.0	0.19777991875880702	7.273751609317544E-4
54-55	0.0	0.0	0.0	0.22789986292284467	7.273751609317544E-4
56-57	0.0	0.0	0.0	0.2614252453403355	7.60437668246834E-4
58-59	0.0	0.0	0.0	0.3031170670646511	7.935001755619138E-4
60-61	0.0	0.0	0.0	0.34447826371581586	9.257502048222328E-4
62-63	0.0	0.0	0.0	0.39112946153739336	9.257502048222328E-4
64-65	0.0	0.0	0.0	0.436590409095628	9.588127121373125E-4
66-67	0.0	0.0	0.0	0.4874736078535358	9.918752194523924E-4
68-69	0.0	0.0	0.0	0.5496311216058857	9.918752194523924E-4
70-71	0.0	0.0	0.0	0.6206493873186769	0.0010580002340825519
72-73	0.0	0.0	0.0	0.6925272782216603	0.0012233127706579505
74-75	0.0	0.0	0.0	0.7642729190953834	0.0013225002926031898
76-77	0.0	0.0	0.0	0.8501031880853304	0.0013225002926031898
78-79	0.0	0.0	0.0	0.9456868967332259	0.0013225002926031898
80-81	0.0	0.0	0.0	1.0466928565807945	0.0013555627999182695
82-83	0.0	0.0	0.0	1.1590723189447507	0.0014547503218635088
84-85	0.0	0.0	0.0	1.2717493438745424	0.0014547503218635088
86-87	0.0	0.0	0.0	1.393948370911077	0.001553937843808748
88-89	0.0	0.0	0.0	1.5289425882785477	0.001653125365753987
90-91	0.0	0.0	0.0	1.6875434358689851	0.001653125365753987
92-93	0.0	0.0	0.0	1.8513681596152054	0.001653125365753987
94-95	0.0	0.0	0.0	2.023756072756031	0.0017192503803841466
96-97	0.0	0.0	0.0	2.2033846749988593	0.0018515004096444655
98-99	0.0	0.0	0.0	2.4040410318940784	0.0018515004096444655
100-101	0.0	0.0	0.0	2.638255833714103	0.0018515004096444655
102-103	0.0	0.0	0.0	2.883976388079776	0.001917625424274625
104-105	0.0	0.0	0.0	3.1459967585517825	0.001917625424274625
106-107	3.3062507315079746E-5	0.0	0.0	3.4152908806331075	0.001950687931589705
108-109	6.612501463015949E-5	0.0	0.0	3.6947351924601612	0.0019837504389047847
110-111	6.612501463015949E-5	0.0	0.0	4.0062831988901575	0.002049875453534944
112-113	6.612501463015949E-5	0.0	0.0	4.347488274381781	0.002049875453534944
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2320	0.0	83.507416	1
GTATCAA	4620	0.0	58.321156	1
TCAACGC	6135	0.0	43.096085	4
CAACGCA	6230	0.0	42.316727	5
ATCAACG	6390	0.0	41.562668	3
TATCAAC	6515	0.0	40.857983	2
AACGCAG	6540	0.0	40.482166	6
GTACATG	7770	0.0	36.825603	1
ACGCAGA	7530	0.0	34.921623	7
TACATGG	8125	0.0	34.52084	2
ACATGGG	8245	0.0	33.65617	3
CGCAGAG	7990	0.0	32.910027	8
CATGGGG	6905	0.0	31.304903	4
GCAGAGT	8825	0.0	29.053669	9
ATGGGGG	3725	0.0	28.756937	5
CAGAGTA	8525	0.0	26.447851	10-11
AGAGTAC	8165	0.0	26.411764	10-11
GAGTACT	5025	0.0	25.217167	12-13
CGTATAC	145	1.3329847E-4	24.640678	3
GTACTTT	5220	0.0	24.218163	14-15
>>END_MODULE