FastQCFastQC Report
Mon 15 Aug 2016
SRR2921522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840759
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.11082385037910993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.073.999291
GTATCAA40250.058.110231
TCAACGC53050.043.7408334
CAACGCA54400.042.87415
AACGCAG57000.040.9162146
GTACATG98550.040.0389631
ATCAACG57550.040.0061043
TATCAAC60300.038.7735752
TACATGG102150.038.5549622
ACATGGG103550.037.4591673
CATGGGG86450.035.582254
ACGCAGA67050.034.250967
ATGGGGG50350.032.7331735
CGCAGAG72500.031.5924538
GCAGAGT79450.028.0059389
AGAGTAC71250.025.6331710-11
CAGAGTA76350.024.70011910-11
GCGCGAC2502.0125299E-823.797569
GTATAAA7900.023.3539581
ATGGGGA33000.020.5541135
Produced by FastQC (version 0.11.5)