##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921522_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1840759
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.686964453249992	31.0	30.0	34.0	25.0	34.0
2	29.79046686719989	31.0	30.0	34.0	25.0	34.0
3	29.87825619757937	31.0	30.0	34.0	25.0	34.0
4	33.24149603505945	37.0	35.0	37.0	26.0	37.0
5	33.26543724626635	37.0	35.0	37.0	27.0	37.0
6	33.28467822240717	37.0	35.0	37.0	26.0	37.0
7	33.1286137946358	37.0	35.0	37.0	26.0	37.0
8	33.057860371727095	37.0	35.0	37.0	25.0	37.0
9	34.4843762817403	39.0	35.0	39.0	25.0	39.0
10-11	34.360952737430594	39.0	35.0	39.0	25.0	39.0
12-13	34.1983885451599	39.0	35.0	39.0	23.0	39.0
14-15	35.31974038969795	40.0	36.0	41.0	23.5	41.0
16-17	35.24700028629495	40.0	35.0	41.0	23.0	41.0
18-19	35.2702933952788	40.0	35.0	41.0	23.0	41.0
20-21	35.291991238396776	40.0	35.0	41.0	23.0	41.0
22-23	35.262591409304534	40.0	35.0	41.0	23.5	41.0
24-25	35.21993998127947	40.0	35.0	41.0	24.0	41.0
26-27	35.14238229990998	40.0	35.0	41.0	24.0	41.0
28-29	35.09374991511653	40.0	35.0	41.0	24.0	41.0
30-31	34.99262070700184	40.0	35.0	41.0	24.0	41.0
32-33	34.87124849043248	40.0	34.5	41.0	23.0	41.0
34-35	34.700899465926824	39.0	34.0	41.0	22.0	41.0
36-37	34.56261112943085	39.0	34.0	41.0	21.0	41.0
38-39	34.43270466150104	39.0	34.0	41.0	19.5	41.0
40-41	34.27308843797586	39.0	33.5	41.0	18.0	41.0
42-43	34.0785325509749	39.0	33.0	41.0	15.5	41.0
44-45	33.883616758087285	39.0	33.0	41.0	15.0	41.0
46-47	33.626499449411895	38.0	33.0	41.0	12.5	41.0
48-49	33.46525427826239	38.0	33.0	40.5	9.0	41.0
50-51	33.266575907003585	38.0	33.0	40.0	8.0	41.0
52-53	33.08074115079704	38.0	32.5	40.0	7.5	41.0
54-55	32.881046894243084	38.0	32.0	40.0	6.5	41.0
56-57	32.642239152436574	37.0	32.0	40.0	2.0	41.0
58-59	32.40761799887981	37.0	32.0	40.0	2.0	41.0
60-61	32.102314045456254	36.0	31.0	40.0	2.0	41.0
62-63	31.7909275467348	36.0	31.0	39.0	2.0	41.0
64-65	31.489397308392896	35.0	31.0	39.0	2.0	41.0
66-67	31.445901120135773	35.0	31.0	39.0	2.0	41.0
68-69	31.20158722570418	35.0	31.0	38.5	2.0	40.5
70-71	30.9006485911518	35.0	31.0	37.0	2.0	40.0
72-73	30.57632069162775	35.0	31.0	37.0	2.0	39.0
74-75	30.277637105128917	35.0	30.0	36.5	2.0	39.0
76-77	29.99358878593015	35.0	30.0	36.0	2.0	39.0
78-79	29.715248981534245	35.0	30.0	35.5	2.0	37.0
80-81	29.476035157236772	35.0	30.0	35.0	2.0	37.0
82-83	29.277858481202593	35.0	30.0	35.0	2.0	36.5
84-85	29.077792367170282	35.0	30.0	35.0	2.0	36.0
86-87	28.92125883942439	34.0	29.0	35.0	2.0	36.0
88-89	28.793106267577667	34.0	29.0	35.0	2.0	35.5
90-91	28.68248179147841	34.0	29.0	35.0	2.0	35.0
92-93	28.579681805168413	34.0	29.0	35.0	2.0	35.0
94-95	28.513172555451312	34.0	29.0	35.0	2.0	35.0
96-97	28.408623290718666	34.0	29.0	35.0	2.0	35.0
98-99	28.34634191656811	34.0	29.0	35.0	2.0	35.0
100-101	28.281775615384742	34.0	29.0	35.0	2.0	35.0
102-103	27.316909220598674	32.5	26.0	34.5	2.0	35.0
104-105	28.123615041404115	34.0	29.0	35.0	2.0	35.0
106-107	28.438216789921984	34.0	29.0	35.0	2.0	35.0
108-109	28.474287508576623	35.0	29.0	35.0	2.0	35.0
110-111	28.414876417825475	35.0	29.0	35.0	2.0	35.0
112-113	28.34063992081527	35.0	29.0	35.0	2.0	35.0
114-115	28.222411244492083	34.5	29.0	35.0	2.0	35.0
116-117	28.15820675058495	34.0	29.0	35.0	2.0	35.0
118-119	28.001994557679737	34.0	29.0	35.0	2.0	35.0
120-121	27.828201573372723	34.0	29.0	35.0	2.0	35.0
122-123	27.696416260901074	34.0	29.0	35.0	2.0	35.0
124-125	26.86600011191036	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78095.0
3	13678.0
4	8799.0
5	7322.0
6	7953.0
7	8942.0
8	9966.0
9	10473.0
10	11097.0
11	11775.0
12	11890.0
13	12593.0
14	14138.0
15	12081.0
16	8460.0
17	7729.0
18	6713.0
19	6488.0
20	6144.0
21	6472.0
22	7487.0
23	10179.0
24	14845.0
25	20043.0
26	24020.0
27	25503.0
28	29651.0
29	35085.0
30	39462.0
31	47250.0
32	58936.0
33	76140.0
34	106049.0
35	164805.0
36	312339.0
37	469328.0
38	138644.0
39	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.023942912635924	17.05327947473191	12.151769959356066	24.771007653276104
2	16.781884515525338	19.488371890829775	38.49486111367714	25.234882479967744
3	18.8690195115245	24.828438018428546	28.945816898033307	27.356725572013644
4	13.175065605244724	15.27030338995301	35.720337163951434	35.83429384085083
5	14.09329922006104	37.54358354563556	33.310327887382726	15.052789346920676
6	33.0353687752999	34.68385719441196	17.757597036405773	14.52317699388237
7	29.429146107705677	31.460957493697123	21.037611825440088	18.072284573157113
8	27.847469076392084	33.502110484272855	19.581718157555652	19.068702281779405
9	28.17599126184311	14.424860273719503	18.804481022494777	38.59466744194261
10-11	26.305862980645127	24.922653870295445	27.082775167721408	21.68870798133802
12-13	26.527840122948902	22.812064904674383	27.09476152021016	23.565333452166552
14-15	24.12548250693294	23.760182030716326	24.620610837609753	27.493724624740985
16-17	24.11406410997735	26.71081491754742	24.984185538952485	24.190935433522746
18-19	24.19889376640932	25.864413145335767	26.61061670090412	23.326076387350792
20-21	25.45038311510413	24.92036554660219	26.068052940332322	23.56119839796136
22-23	25.180435828928598	24.514351296607774	26.00181054108871	24.303402333374915
24-25	24.55578673849495	24.86727215154608	26.13520260821468	24.441738501744293
26-27	24.23277763208492	24.936279378813964	26.232976015935932	24.597966973165185
28-29	24.271294812196274	25.23698646591368	26.133597264593806	24.358121457296235
30-31	24.784577405384724	24.935348763392238	26.076297698970365	24.203776132252678
32-33	24.41884893591867	25.01290269101622	26.016798637845444	24.55144973521966
34-35	24.603734455619236	25.112442352235316	26.127983200493027	24.15583999165242
36-37	24.69556133329311	24.972055697824867	25.994057572464268	24.338325396417748
38-39	24.685546428564635	25.12554633072044	25.879055336055394	24.30985190465953
40-41	24.63939678297661	24.956457485956197	26.07229810173934	24.331847629327857
42-43	24.55589492045218	25.061898309027885	25.834410948241327	24.54779582227861
44-45	24.379468645184645	25.129213099899523	25.940353651301155	24.550964603614677
46-47	24.48348188452771	25.119298935122636	25.76471045495451	24.632508725395148
48-49	24.683125489670683	25.317567488611374	25.47121843789651	24.528088583821432
50-51	24.65597664152326	25.497269880196495	25.38217872582814	24.464574752452105
52-53	24.733674771752295	25.314105588617075	25.539880021830026	24.4123396178006
54-55	24.546686069657824	25.2111162290803	25.670075777648243	24.57212192361363
56-57	24.507660070430745	25.36275855472133	25.38729708496935	24.742284289878576
58-59	24.63204492556113	25.235708375171374	25.484549025575664	24.647697673691834
60-61	24.72493298752678	25.411646108929066	25.606522320961556	24.256898582582597
62-63	24.53997199410099	25.431318290873843	25.551102885129346	24.47760682989582
64-65	24.540625	25.10967391304348	25.673940217391305	24.675760869565217
66-67	24.482539275387875	25.337675814371437	25.591491403664605	24.588293506576083
68-69	24.51451331506891	25.322718122236864	25.555275693074	24.60749286962022
70-71	24.460686600221486	25.136144684725082	25.720617709203815	24.68255100584962
72-73	24.60446178571962	25.50714399251161	25.4685608628492	24.41983335891956
74-75	24.45389833492931	25.555231891445956	25.419202387771584	24.57166738585315
76-77	24.520879994543986	25.159462125928005	25.53749774817062	24.782160131357394
78-79	24.438012046752355	25.292881504039855	25.58796389886988	24.681142550337903
80-81	24.526185233134225	25.47877319082053	25.33952977839241	24.65551179765283
82-83	24.646175571898997	25.456884834512454	25.376033966058394	24.520905627530155
84-85	24.46911286072125	25.550366494422438	25.23796327992132	24.742557364934985
86-87	24.236950431526306	25.688230666078404	25.417344945024134	24.657473957371156
88-89	24.354602252412327	25.401067629967972	25.434566911078292	24.809763206541408
90-91	24.495805156494026	25.243686137742127	25.497742909868254	24.762765795895593
92-93	24.50763979807601	25.559370254926183	25.339960894275173	24.593029052722635
94-95	24.396349121442487	25.32164694318927	25.44123475914139	24.840769176226853
96-97	24.38669447705833	25.31700966934502	25.429013685516193	24.86728216808046
98-99	24.277720609248696	25.345926950572707	25.33609299768223	25.040259442496364
100-101	24.22269690007832	25.482377109190125	25.464311822602685	24.830614168128868
102-103	24.325977679050443	25.241144760199873	25.609221754844423	24.823655805905258
104-105	24.252207594718016	25.443074113844983	25.4444866702216	24.8602316212154
106-107	24.21405063858513	25.41591905867268	25.49135672344639	24.8786735792958
108-109	24.276615905539533	25.33496041327541	25.439740811815813	24.948682869369243
110-111	24.19498459238268	25.57933401715048	25.4761909936066	24.749490396860235
112-113	24.15922260892809	25.580701699103237	25.46440923646297	24.795666455505703
114-115	24.014163801896927	25.706729979096576	25.53320031565665	24.745905903349847
116-117	24.048409538261105	25.744704663460155	25.39775804901477	24.809127749263972
118-119	24.195097699143382	25.388762706225158	25.47991916806932	24.936220426562144
120-121	23.923600964746623	25.50794215009399	25.660264825564916	24.908192059594477
122-123	23.91602159506802	25.62115708048157	25.579423280178894	24.88339804427152
124-125	23.886532890891036	25.591924124188075	25.582362502797864	24.939180482123028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	184.5
2	253.5
3	253.0
4	225.0
5	204.5
6	204.5
7	261.0
8	360.0
9	433.0
10	448.5
11	519.0
12	630.0
13	713.5
14	818.0
15	905.5
16	998.5
17	1149.5
18	1292.0
19	1393.5
20	1569.5
21	1788.0
22	2076.5
23	2487.0
24	2941.0
25	3736.5
26	4927.5
27	6322.0
28	7422.0
29	8960.0
30	11369.0
31	13480.5
32	15869.0
33	18456.5
34	21248.5
35	24337.5
36	27550.0
37	30723.0
38	33265.0
39	36148.0
40	38996.0
41	41424.0
42	43998.5
43	48966.5
44	54323.5
45	57865.5
46	62018.5
47	66268.0
48	71401.5
49	76273.0
50	77437.0
51	77105.5
52	76684.5
53	76893.5
54	77722.0
55	76388.5
56	72114.0
57	66181.5
58	61636.0
59	56944.5
60	50550.0
61	44742.0
62	38774.5
63	30856.0
64	23633.5
65	18572.0
66	14262.0
67	10948.0
68	8622.5
69	6843.0
70	5826.5
71	4842.0
72	3769.0
73	3344.5
74	2761.5
75	2061.0
76	1449.5
77	848.0
78	485.0
79	331.0
80	200.5
81	78.5
82	51.0
83	34.5
84	23.0
85	22.5
86	20.5
87	15.0
88	16.0
89	12.0
90	6.0
91	6.5
92	8.0
93	9.0
94	6.5
95	5.0
96	9.5
97	10.5
98	9.5
99	12.0
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03433366345078307
2	0.02591322383864482
3	0.025098342585857244
4	0.03183469427556785
5	0.03341013136429049
6	0.04514442140443154
7	0.03944025263491853
8	0.03585477512265321
9	0.030096280936287696
10-11	0.035528822621538184
12-13	0.03503989386986564
14-15	0.03422501261707806
16-17	0.036452354708030765
18-19	0.04957194287791069
20-21	0.05992093478831286
22-23	0.0531030949733235
24-25	0.06445710709549703
26-27	0.04843110912400809
28-29	0.04813231933131931
30-31	0.04818664474817182
32-33	0.055765040399096244
34-35	0.03829941888101593
36-37	0.050984403716075814
38-39	0.0597307958293291
40-41	0.048376783707155584
42-43	0.05693303686142509
44-45	0.05788373165634393
46-47	0.06288167000677439
48-49	0.04750757703751551
50-51	0.049599105586336935
52-53	0.059269029786082804
54-55	0.04544321119712032
56-57	0.04321586910616762
58-59	0.04489995702859527
60-61	0.033491619489569247
62-63	0.04288991660505259
64-65	0.04123299139105119
66-67	0.03495840574458688
68-69	0.030938324897501517
70-71	0.04870273620827061
72-73	0.031318602815469054
74-75	0.038951323883245986
76-77	0.03197050781769911
78-79	0.03232362302724039
80-81	0.024799552793168467
82-83	0.018117526520310373
84-85	0.018959570481524197
86-87	0.01711250630853903
88-89	0.022871000494904547
90-91	0.012250381500239845
92-93	0.019747289025885517
94-95	0.014695025258602565
96-97	0.010865083370500973
98-99	0.010702107119943457
100-101	0.011544151081157283
102-103	0.006057283979054292
104-105	0.006763514398136856
106-107	0.009045181905942059
108-109	0.013201076295158681
110-111	0.006030121270628039
112-113	0.007659883776203185
114-115	0.008230300653154487
116-117	0.0070623041908256315
118-119	0.23664151580951118
120-121	0.3528435824570191
122-123	0.028466518430712545
124-125	0.0036398029291178262
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1840759.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.21454937102199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91068129524817	47.91089532200498
2	9.976121971559483	11.814631340324926
3	3.239605115529684	5.754952711684436
4	1.6667669166598706	3.94787407506168
5	0.9931252861141181	2.9403733143107393
6	0.6540419250095787	2.323727871551877
7	0.44637083645699277	1.8502153553216887
8	0.3429680367739743	1.624695819698799
9	0.2640952657306321	1.4074453936133717
>10	1.4119380962751484	14.85227058253495
>50	0.06981134915340302	2.767478997297721
>100	0.023732504591009437	2.356050234420928
>500	4.633755612726787E-4	0.1824116469344347
>1k	2.7802533676360723E-4	0.266977335239478
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2040	0.11082385037910993	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.4325416852504865E-5
5	0.0	0.0	0.0	0.0	5.4325416852504865E-5
6	0.0	0.0	0.0	0.0	5.4325416852504865E-5
7	0.0	0.0	0.0	0.0	5.4325416852504865E-5
8	0.0	0.0	0.0	0.0	5.4325416852504865E-5
9	0.0	0.0	0.0	0.0	1.0865083370500973E-4
10-11	0.0	0.0	0.0	0.0	1.0865083370500973E-4
12-13	0.0	0.0	0.0	5.4325416852504865E-5	1.3581354213126217E-4
14-15	0.0	0.0	0.0	5.4325416852504865E-5	2.444643758362719E-4
16-17	0.0	0.0	0.0	8.14881252787573E-5	3.259525011150292E-4
18-19	0.0	0.0	0.0	1.0865083370500973E-4	3.8027791796753406E-4
20-21	0.0	0.0	0.0	1.9013895898376703E-4	3.8027791796753406E-4
22-23	0.0	0.0	0.0	3.8027791796753406E-4	4.0744062639378647E-4
24-25	0.0	0.0	0.0	4.0744062639378647E-4	4.346033348200389E-4
26-27	0.0	0.0	0.0	5.160914600987962E-4	4.889287516725438E-4
28-29	0.0	0.0	0.0	0.0010865083370500972	4.889287516725438E-4
30-31	0.0	0.0	0.0	0.0037484537628228354	4.889287516725438E-4
32-33	0.0	0.0	0.0	0.009208158156499575	5.432541685250486E-4
34-35	0.0	0.0	0.0	0.01643343859788272	5.432541685250486E-4
36-37	0.0	0.0	0.0	0.027325684676809944	5.432541685250486E-4
38-39	0.0	0.0	0.0	0.04487279432016902	5.975795853775535E-4
40-41	0.0	0.0	0.0	0.06739067960553229	6.519050022300584E-4
42-43	0.0	0.0	0.0	0.08901219551282921	6.519050022300584E-4
44-45	0.0	0.0	0.0	0.11199184684143877	6.519050022300584E-4
46-47	0.0	0.0	0.0	0.1389915790171337	7.062304190825632E-4
48-49	0.0	0.0	0.0	0.1683273041174863	7.877185443613205E-4
50-51	0.0	0.0	0.0	0.2015744592312193	8.692066696400778E-4
52-53	0.0	0.0	0.0	0.23818979018980757	9.235320864925828E-4
54-55	0.0	0.0	0.0	0.27711395136462735	9.778575033450876E-4
56-57	0.0	0.0	0.0	0.31557634649620075	0.00100502021177134
58-59	0.0	0.0	0.0	0.36400745562020886	0.0010865083370500972
60-61	0.0	0.0	0.0	0.4127645172453319	0.0011408337539026022
62-63	0.0	0.0	0.0	0.46562314784281916	0.0013038100044601168
64-65	0.0	0.0	0.0	0.5222845576199817	0.0013038100044601168
66-67	0.0	0.0	0.0	0.5872577561755776	0.0013038100044601168
68-69	0.0	0.0	0.0	0.6606242316348854	0.0013038100044601168
70-71	0.0	0.0	0.0	0.7432803533759715	0.0013038100044601168
72-73	0.0	0.0	0.0	0.826941495328829	0.0015211116718701363
74-75	0.0	0.0	0.0	0.9132645827074592	0.001575437088722641
76-77	0.0	0.0	0.0	1.0122726549211494	0.001575437088722641
78-79	0.0	0.0	0.0	1.1224989257148819	0.0016025997971488935
80-81	0.0	0.0	0.0	1.240792520911211	0.0016569252140013983
82-83	0.0	2.7162708426252433E-5	0.0	1.3704672909381403	0.0017384133392801557
84-85	0.0	5.4325416852504865E-5	0.0	1.504841209522811	0.0017384133392801557
86-87	0.0	5.4325416852504865E-5	0.0	1.6508407673139178	0.001765576047706408
88-89	0.0	5.4325416852504865E-5	0.0	1.808846242229428	0.0017927387561326605
90-91	0.0	5.4325416852504865E-5	0.0	1.9854853351253476	0.0017927387561326605
92-93	0.0	5.4325416852504865E-5	0.0	2.172880860558063	0.0018470641729851653
94-95	0.0	5.4325416852504865E-5	0.0	2.3685066866439333	0.0019013895898376703
96-97	0.0	5.4325416852504865E-5	0.0	2.580158510701292	0.001955715006690175
98-99	0.0	5.4325416852504865E-5	0.0	2.815007287754671	0.00201004042354268
100-101	0.0	8.14881252787573E-5	0.0	3.0801153219948945	0.00201004042354268
102-103	0.0	1.0865083370500973E-4	0.0	3.3657855265137915	0.0020643658403951847
104-105	0.0	1.0865083370500973E-4	0.0	3.665091410662667	0.0021186912572476895
106-107	0.0	1.0865083370500973E-4	0.0	3.981944404454901	0.002145853965673942
108-109	0.0	1.0865083370500973E-4	0.0	4.309418017241801	0.0022816675078052044
110-111	0.0	1.0865083370500973E-4	0.0	4.667232375340824	0.0024174810499364664
112-113	2.7162708426252433E-5	1.3581354213126217E-4	0.0	5.049357357481343	0.002444643758362719
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2075	0.0	73.99929	1
GTATCAA	4025	0.0	58.11023	1
TCAACGC	5305	0.0	43.740833	4
CAACGCA	5440	0.0	42.8741	5
AACGCAG	5700	0.0	40.916214	6
GTACATG	9855	0.0	40.038963	1
ATCAACG	5755	0.0	40.006104	3
TATCAAC	6030	0.0	38.773575	2
TACATGG	10215	0.0	38.554962	2
ACATGGG	10355	0.0	37.459167	3
CATGGGG	8645	0.0	35.58225	4
ACGCAGA	6705	0.0	34.25096	7
ATGGGGG	5035	0.0	32.733173	5
CGCAGAG	7250	0.0	31.592453	8
GCAGAGT	7945	0.0	28.005938	9
AGAGTAC	7125	0.0	25.63317	10-11
CAGAGTA	7635	0.0	24.700119	10-11
GCGCGAC	250	2.0125299E-8	23.79756	9
GTATAAA	790	0.0	23.353958	1
ATGGGGA	3300	0.0	20.554113	5
>>END_MODULE