FastQCFastQC Report
Mon 15 Aug 2016
SRR2921522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840759
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34020.18481506813222154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.16580117223384483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.081.966651
GTATCAA57300.058.155621
TCAACGC77800.042.4767884
CAACGCA79350.041.7005545
ATCAACG82150.040.227563
AACGCAG83000.040.0709536
TATCAAC86100.038.45122
GTACATG100450.037.5060771
TACATGG102650.036.544322
ACATGGG106300.034.7853663
ACGCAGA97750.034.0188947
CGCAGAG103150.032.23718
CATGGGG87650.031.453384
GCAGAGT110400.029.5273749
AGAGTAC101550.027.88343410-11
ATGGGGG49300.027.5233885
CAGAGTA107800.025.57703810-11
GTATAGG5400.025.3903431
TATCACG1651.1039521E-525.2611062
GAGTACT58050.024.4405712-13
Produced by FastQC (version 0.11.5)