##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1840759
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18016807197466	31.0	31.0	34.0	28.0	34.0
2	31.24298183521037	33.0	31.0	34.0	28.0	34.0
3	31.335084603687935	33.0	31.0	34.0	28.0	34.0
4	34.839826397697905	37.0	35.0	37.0	32.0	37.0
5	34.48682527153201	37.0	35.0	37.0	30.0	37.0
6	34.45700605022167	37.0	35.0	37.0	30.0	37.0
7	34.302115051454315	37.0	35.0	37.0	30.0	37.0
8	34.25896545935671	37.0	35.0	37.0	30.0	37.0
9	35.8201312610722	39.0	37.0	39.0	30.0	39.0
10-11	35.69108503611825	39.0	36.0	39.0	30.0	39.0
12-13	35.588267394047776	39.0	35.0	39.0	30.0	39.0
14-15	36.755728479393554	40.0	37.0	41.0	30.0	41.0
16-17	36.74128498081498	40.0	37.0	41.0	30.0	41.0
18-19	36.765602123906504	40.0	37.0	41.0	30.0	41.0
20-21	36.75452870256237	40.0	37.0	41.0	30.0	41.0
22-23	36.69168152919529	40.0	37.0	41.0	30.0	41.0
24-25	36.625340416643354	40.0	36.0	41.0	29.5	41.0
26-27	36.4993182160185	40.0	36.0	41.0	29.0	41.0
28-29	36.31800795215452	40.0	36.0	41.0	28.5	41.0
30-31	36.25932129083709	40.0	36.0	41.0	29.0	41.0
32-33	36.131993378818194	40.0	35.0	41.0	28.5	41.0
34-35	35.996431634994046	40.0	35.0	41.0	28.0	41.0
36-37	35.86095898485353	40.0	35.0	41.0	27.0	41.0
38-39	35.68981898227851	40.0	35.0	41.0	27.0	41.0
40-41	35.484721519764406	39.5	35.0	41.0	26.0	41.0
42-43	35.310269839778044	39.0	35.0	41.0	25.5	41.0
44-45	35.087871905013095	39.0	35.0	41.0	24.5	41.0
46-47	34.79964623288546	39.0	34.5	41.0	23.5	41.0
48-49	34.537673318451795	39.0	34.0	41.0	22.5	41.0
50-51	34.31125829073768	39.0	34.0	41.0	22.0	41.0
52-53	34.11794808554515	38.0	34.0	41.0	21.0	41.0
54-55	33.900238977508735	38.0	33.0	40.0	20.0	41.0
56-57	33.643406334017655	37.5	33.0	40.0	19.5	41.0
58-59	33.50014206096507	37.0	33.0	40.0	19.5	41.0
60-61	33.38248352989174	37.0	33.0	40.0	19.0	41.0
62-63	33.10840962885418	36.5	33.0	40.0	18.0	41.0
64-65	32.818696798440214	36.0	33.0	39.0	17.5	41.0
66-67	32.517082355702186	35.5	32.5	39.0	16.0	41.0
68-69	32.202836438664704	35.0	32.0	39.0	13.5	41.0
70-71	31.90421695615776	35.0	32.0	37.5	13.0	40.0
72-73	31.592162254809022	35.0	32.0	37.0	11.0	39.5
74-75	31.30368587088261	35.0	32.0	36.5	9.0	39.0
76-77	31.012455188321773	35.0	31.5	36.0	7.5	39.0
78-79	30.736762118234925	35.0	31.0	36.0	7.0	37.5
80-81	30.512939771040095	35.0	31.0	35.0	6.5	37.0
82-83	30.32475707031719	35.0	31.0	35.0	3.5	36.5
84-85	30.132336172198535	35.0	31.0	35.0	2.0	36.0
86-87	29.985839536843223	35.0	31.0	35.0	2.0	36.0
88-89	29.87678099088474	35.0	31.0	35.0	2.0	36.0
90-91	29.775051215286737	35.0	31.0	35.0	2.0	35.0
92-93	29.833909001667248	35.0	31.0	35.0	2.0	35.0
94-95	29.80080499402692	35.0	31.0	35.0	2.0	35.0
96-97	29.77829878870618	35.0	31.0	35.0	2.0	35.0
98-99	29.738326690240278	35.0	31.0	35.0	2.0	35.0
100-101	29.69184124592084	35.0	31.0	35.0	2.0	35.0
102-103	28.8141196104433	33.5	29.5	35.0	2.0	35.0
104-105	29.514421768411836	34.5	31.0	35.0	2.0	35.0
106-107	29.615923377259055	35.0	31.0	35.0	2.0	35.0
108-109	29.622257720864056	35.0	31.0	35.0	2.0	35.0
110-111	29.591496225198412	35.0	31.0	35.0	2.0	35.0
112-113	29.545432617740836	35.0	31.0	35.0	2.0	35.0
114-115	29.480763641519612	35.0	31.0	35.0	2.0	35.0
116-117	29.40295334696177	35.0	31.0	35.0	2.0	35.0
118-119	29.313464717543145	35.0	31.0	35.0	2.0	35.0
120-121	29.19142538485483	35.0	31.0	35.0	2.0	35.0
122-123	29.068296555931546	35.0	31.0	35.0	2.0	35.0
124-125	28.16785168509294	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33306.0
3	12036.0
4	6447.0
5	5499.0
6	5798.0
7	6758.0
8	7757.0
9	8790.0
10	10290.0
11	11320.0
12	11829.0
13	13401.0
14	17171.0
15	14211.0
16	8136.0
17	6840.0
18	6034.0
19	5496.0
20	5378.0
21	5456.0
22	6543.0
23	8726.0
24	12373.0
25	17004.0
26	21547.0
27	23909.0
28	27496.0
29	32725.0
30	38134.0
31	45761.0
32	56850.0
33	73482.0
34	102340.0
35	162562.0
36	318978.0
37	521230.0
38	168706.0
39	440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03783245154612	17.613153324365882	12.39950372613341	24.949510497954584
2	16.92125824267164	19.90747129121879	37.65628344468433	25.514987021425238
3	18.748325741550463	24.98762475093283	29.288723390497655	26.97532611701905
4	13.231645106566631	15.712755219348812	35.47852659695033	35.57707307713422
5	14.026475362132985	37.480118482163284	33.28292425542459	15.210481900279143
6	33.13353861602085	35.15207926150396	17.07791124593768	14.636470876537514
7	29.068246104561513	31.815074931295324	20.895024466363775	18.22165449777939
8	27.982651959695087	33.56611101724427	19.247186756554274	19.20405026650636
9	28.186060662034347	14.106014592353954	18.71070087847492	38.99722386713678
10-11	26.395617798821323	24.877059589732305	26.86369186595332	21.863630745493047
12-13	26.802228960073972	22.6741870798765	27.229326786473905	23.294257173575623
14-15	24.258496013612202	23.520054718269094	24.962113628961564	27.259335639157133
16-17	23.935910262849852	26.467917235668953	25.383962787725906	24.21220971375529
18-19	24.014429606771483	25.303369443569157	27.49899492573316	23.1832060239262
20-21	25.225491256084503	24.25275884157721	27.225016394671776	23.296733507666513
22-23	24.91186922008912	23.95606079251283	27.100408374821424	24.03166161257663
24-25	24.283157943636052	24.41100221377594	27.171226835017592	24.13461300757041
26-27	23.98284471522586	24.45698384058668	27.30801222862775	24.25215921555972
28-29	24.177446998907094	24.688475360967605	27.204280130801095	23.92979750932421
30-31	24.51230074723861	24.456306754706645	27.135324520641436	23.89606797741331
32-33	24.14825415634694	24.506738146135742	27.095423950240377	24.249583747276937
34-35	24.29109320243329	24.65153379466759	27.1673640292406	23.89000897365852
36-37	24.516630476152702	24.522579973881978	26.911371228826646	24.049418321138674
38-39	24.470326780338844	24.73453851921886	26.71355915626121	24.081575544181096
40-41	24.4655893607392	24.612879968748963	26.812459557563134	24.109071112948698
42-43	24.421373924698543	24.709569792487564	26.59347811854741	24.275578164266484
44-45	24.3342242400895	24.715055635940615	26.58063503051161	24.370085093458275
46-47	24.48109581002627	24.791071821523364	26.21970534620327	24.508127022247095
48-49	24.684710139808843	25.128995799757657	25.74139982720866	24.44489423322484
50-51	24.614627711162175	25.3392433440388	25.615718179857243	24.430410764941783
52-53	24.726256227377068	25.20920482198742	25.664495806937932	24.400043143697584
54-55	24.54204552771227	25.12139084169841	25.90375831425048	24.432805316338843
56-57	24.446512861223543	25.18437798342405	25.68905718773925	24.680051967613156
58-59	24.518961058577716	25.051189384013156	25.893963469647286	24.535886087761845
60-61	24.71003586176918	25.157438600304282	25.917762442947183	24.214763094979354
62-63	24.582268548982615	25.249150029043278	25.85954790293405	24.309033519040053
64-65	24.49215503720968	24.86923703651583	26.014745862217826	24.623862064056667
66-67	24.531300384206954	25.154594197532305	25.837815928927554	24.476289489333183
68-69	24.527124394675756	25.12171690386973	25.805070378452633	24.546088323001886
70-71	24.428372025043345	24.96605521684726	25.98722404752215	24.61834871058724
72-73	24.65970874986518	25.335306044107252	25.565956720804827	24.439028485222735
74-75	24.524165743193628	25.43808483418644	25.480873852269486	24.556875570350442
76-77	24.526524798649945	25.055181611001494	25.599377565011068	24.818916025337494
78-79	24.449103888700808	25.179474945781205	25.63102200867392	24.740399156844067
80-81	24.521692258914452	25.352830153308968	25.375624501288733	24.749853086487843
82-83	24.594546431503325	25.330423401588508	25.414907043545202	24.660123123362958
84-85	24.418403430995284	25.380851180009078	25.321302645916322	24.879442743079316
86-87	24.253021477763355	25.465850489383097	25.45262061529509	24.82850741755846
88-89	24.31917635274298	25.234982947995803	25.47821818034617	24.96762251891505
90-91	24.322043107148485	25.149033158927047	25.574617093989232	24.954306639935236
92-93	24.428344175706204	25.354473334499612	25.410052493238684	24.807129996555506
94-95	24.329020862954064	25.14159546372956	25.455726867579653	25.07365680573673
96-97	24.285222240089706	25.183135656202598	25.507140715114584	25.024501388593112
98-99	24.198485930891735	25.190942748412997	25.30874895082671	25.30182236986856
100-101	24.174059596746307	25.308772605631948	25.438326025296192	25.078841772325553
102-103	24.283072436494336	25.0858639801554	25.58295693708161	25.048106646268653
104-105	24.226783121160807	25.215961806228982	25.381033172363626	25.17622190024659
106-107	24.234763516807917	25.20355104387902	25.421423660786925	25.140261778526142
108-109	24.294645168844767	25.120469505736104	25.305782128756004	25.279103196663122
110-111	24.19812489270666	25.31544241797356	25.379275164226716	25.10715752509306
112-113	24.17487100610619	25.28467758453671	25.389127731255883	25.151323678101217
114-115	23.915698993895575	25.488159890173023	25.454911913233648	25.141229202697758
116-117	23.983064360807564	25.39397826433603	25.330168087794547	25.292789287061858
118-119	23.96074171205319	25.080796564342915	25.515034802897674	25.443426920706223
120-121	23.69653428688931	25.251604154748573	25.625404400200143	25.42645715816197
122-123	23.746101110499342	25.29867605487659	25.509378386020686	25.44584444860338
124-125	23.71930160123978	25.369989197554343	25.405499249045278	25.505209952160595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	54.0
2	111.0
3	160.5
4	113.0
5	98.0
6	185.0
7	405.0
8	658.0
9	767.0
10	664.0
11	548.5
12	629.5
13	743.0
14	906.0
15	1087.5
16	1212.0
17	1405.5
18	1658.5
19	1881.0
20	2085.0
21	2372.0
22	2742.0
23	3173.5
24	3584.0
25	4326.0
26	5636.5
27	6920.5
28	7933.5
29	9456.0
30	11767.0
31	13828.5
32	16059.5
33	18516.0
34	21073.5
35	23934.0
36	27103.0
37	30217.5
38	32632.5
39	35407.5
40	38206.5
41	40783.0
42	43475.0
43	48158.0
44	53482.5
45	57499.0
46	61931.5
47	66092.5
48	71504.0
49	76272.5
50	76945.5
51	77206.5
52	76973.5
53	76851.5
54	78019.5
55	76677.5
56	72004.5
57	66284.0
58	62271.0
59	57511.0
60	50646.0
61	44642.0
62	38658.0
63	30504.5
64	23216.5
65	18312.5
66	13985.5
67	10581.5
68	8345.5
69	6718.5
70	5570.0
71	4558.0
72	3634.0
73	3302.0
74	2695.0
75	2002.5
76	1395.5
77	755.5
78	429.0
79	272.0
80	155.5
81	66.5
82	37.5
83	13.5
84	8.5
85	8.0
86	4.5
87	2.0
88	1.5
89	1.0
90	1.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12266679125295599
2	0.0017927387561326605
3	0.021295563406181908
4	0.05530327435584995
5	0.026184850922907343
6	0.020100404235426802
7	0.014830838800733827
8	0.004889287516725438
9	0.003748453762822836
10-11	0.0071437923161043896
12-13	0.0043460333482003895
14-15	0.0031237114690190293
16-17	0.009968713992434642
18-19	0.005378216268397982
20-21	0.012060242541256079
22-23	0.00926248357335208
24-25	0.01295661191932241
26-27	0.008692066696400779
28-29	0.013825818588962488
30-31	0.02080663465450936
32-33	0.012522008584502371
34-35	0.02023621777755806
36-37	0.01453204900804505
38-39	0.020589332987099345
40-41	0.009778575033450875
42-43	0.00926248357335208
44-45	0.016949530057981516
46-47	0.01597167255463643
48-49	0.021132587155624393
50-51	0.014450560882766294
52-53	0.02159435319887068
54-55	0.017356970684375302
56-57	0.02075230923765686
58-59	0.015727208178800158
60-61	0.019502824650049246
62-63	0.021838817574706956
64-65	0.01686804193270276
66-67	0.011652801914862293
68-69	0.022979651328609556
70-71	0.014667862550176314
72-73	0.017031018183260277
74-75	0.018117526520310373
76-77	0.013336889837289943
78-79	0.028792470931827577
80-81	0.02121407528090315
82-83	0.009018019197515806
84-85	0.013228239003584935
86-87	0.012359032333944857
88-89	0.019529987358475498
90-91	0.012440520459223616
92-93	0.007659883776203185
94-95	0.006953653357120623
96-97	0.0025532945920677284
98-99	0.0013038100044601166
100-101	0.01116387316318975
102-103	0.0030965487605927773
104-105	0.0021730166741001948
106-107	2.444643758362719E-4
108-109	0.01931268569106548
110-111	5.975795853775535E-4
112-113	0.009126670031220818
114-115	0.002471806466788971
116-117	0.007795697318334448
118-119	0.009751412325024623
120-121	0.0038027791796753406
122-123	5.704168769513011E-4
124-125	0.023631556330839614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1840759.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.486744539524956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.15425846992493	46.07807380415629
2	10.2551418991689	11.790694451482027
3	3.4138003905207555	5.887448128763916
4	1.7351399134178662	3.989901797719453
5	1.0494247236293932	3.016400550037226
6	0.6662230060136165	2.2979395051835505
7	0.4771979916639071	1.9202791329090172
8	0.34144755300612123	1.570296660264704
9	0.2743313041480632	1.419337224067899
>10	1.52567196186067	15.58652488226816
>50	0.07860332039854757	3.004246399218865
>100	0.027905450177904734	2.77488022477519
>500	4.744533719252305E-4	0.15573899258823493
>1k	3.7956269754018426E-4	0.5082382465655286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3402	0.18481506813222154	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3052	0.16580117223384483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.4325416852504865E-5
2	0.0	0.0	0.0	0.0	5.4325416852504865E-5
3	0.0	0.0	0.0	0.0	5.4325416852504865E-5
4	0.0	0.0	0.0	0.0	5.4325416852504865E-5
5	0.0	0.0	0.0	0.0	5.4325416852504865E-5
6	0.0	0.0	0.0	0.0	5.4325416852504865E-5
7	0.0	0.0	0.0	0.0	5.4325416852504865E-5
8	0.0	0.0	0.0	0.0	5.4325416852504865E-5
9	0.0	0.0	0.0	0.0	5.4325416852504865E-5
10-11	0.0	0.0	0.0	0.0	5.4325416852504865E-5
12-13	0.0	0.0	0.0	0.0	2.716270842625243E-4
14-15	0.0	0.0	0.0	0.0	3.5311520954128166E-4
16-17	0.0	0.0	0.0	2.7162708426252433E-5	3.8027791796753406E-4
18-19	0.0	0.0	0.0	5.4325416852504865E-5	3.8027791796753406E-4
20-21	0.0	0.0	0.0	8.14881252787573E-5	3.8027791796753406E-4
22-23	0.0	0.0	0.0	1.629762505575146E-4	4.889287516725438E-4
24-25	0.0	0.0	0.0	1.9013895898376703E-4	5.975795853775535E-4
26-27	0.0	0.0	0.0	2.9878979268877675E-4	5.975795853775535E-4
28-29	0.0	0.0	0.0	9.506947949188352E-4	5.975795853775535E-4
30-31	0.0	0.0	0.0	0.003748453762822836	5.975795853775535E-4
32-33	0.0	0.0	0.0	0.009316808990204584	6.519050022300584E-4
34-35	0.0	0.0	0.0	0.016705065682145245	7.062304190825632E-4
36-37	0.0	0.0	0.0	0.02770596259477748	7.062304190825632E-4
38-39	0.0	0.0	0.0	0.04557902473925158	7.605558359350681E-4
40-41	0.0	0.0	0.0	0.06861300148471364	7.605558359350681E-4
42-43	0.0	0.0	0.0	0.09083209697738814	7.877185443613205E-4
44-45	0.0	0.0	0.0	0.11432783976609648	8.420439612138253E-4
46-47	0.0	0.0	0.0	0.1420066396524477	8.692066696400778E-4
48-49	0.0	0.0	0.0	0.17169547996234164	9.778575033450876E-4
50-51	0.0	0.0	0.0	0.2050512859097796	9.778575033450876E-4
52-53	0.0	0.0	0.0	0.24174810499364663	0.0011136710454763498
54-55	0.0	0.0	0.0	0.282030401589779	0.0013309727128863692
56-57	0.0	0.0	0.0	0.320981725473025	0.001385298129738874
58-59	0.0	0.0	0.0	0.3706351564762145	0.0014124608381651264
60-61	0.0	0.0	0.0	0.4199083095614363	0.0014124608381651264
62-63	0.0	0.0	0.0	0.47420656370551495	0.0014124608381651264
64-65	0.0	0.0	0.0	0.5321174580702851	0.0014124608381651264
66-67	0.0	0.0	0.0	0.5983401412134886	0.0014396235465913788
68-69	0.0	0.0	0.0	0.6731462402193877	0.0014667862550176312
70-71	0.0	0.0	0.0	0.7571604973817865	0.0015482743802963887
72-73	0.0	0.0	0.0	0.8408488020430702	0.0016569252140013983
74-75	0.0	0.0	0.0	0.9294535569295057	0.0016840879224276507
76-77	0.0	0.0	0.0	1.0313408762363787	0.0016840879224276507
78-79	0.0	0.0	0.0	1.142843794326145	0.0016840879224276507
80-81	0.0	0.0	0.0	1.2630931045291642	0.001765576047706408
82-83	0.0	0.0	0.0	1.3970595824874414	0.0018470641729851653
84-85	0.0	0.0	0.0	1.5334978669125072	0.0018470641729851653
86-87	0.0	0.0	0.0	1.682403834505223	0.0018470641729851653
88-89	0.0	0.0	0.0	1.8443750648509663	0.0019013895898376703
90-91	0.0	0.0	0.0	2.0265553502658413	0.0019013895898376703
92-93	0.0	0.0	0.0	2.2194649055090863	0.00201004042354268
94-95	0.0	0.0	0.0	2.418621883690369	0.00201004042354268
96-97	0.0	0.0	0.0	2.6339949988021245	0.002145853965673942
98-99	0.0	0.0	0.0	2.8744664564997375	0.0021730166741001943
100-101	0.0	0.0	0.0	3.1466096322223605	0.0021730166741001943
102-103	0.0	0.0	0.0	3.4395322798910666	0.002254504799378952
104-105	0.0	0.0	0.0	3.7457918173970626	0.0022816675078052044
106-107	0.0	0.0	0.0	4.0680501901661215	0.002335992924657709
108-109	0.0	0.0	0.0	4.404215869649422	0.0024989691752152236
110-111	0.0	0.0	0.0	4.771700151948191	0.002526131883641476
112-113	0.0	0.0	0.0	5.165369285169867	0.0025532945920677284
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2880	0.0	81.96665	1
GTATCAA	5730	0.0	58.15562	1
TCAACGC	7780	0.0	42.476788	4
CAACGCA	7935	0.0	41.700554	5
ATCAACG	8215	0.0	40.22756	3
AACGCAG	8300	0.0	40.070953	6
TATCAAC	8610	0.0	38.4512	2
GTACATG	10045	0.0	37.506077	1
TACATGG	10265	0.0	36.54432	2
ACATGGG	10630	0.0	34.785366	3
ACGCAGA	9775	0.0	34.018894	7
CGCAGAG	10315	0.0	32.2371	8
CATGGGG	8765	0.0	31.45338	4
GCAGAGT	11040	0.0	29.527374	9
AGAGTAC	10155	0.0	27.883434	10-11
ATGGGGG	4930	0.0	27.523388	5
CAGAGTA	10780	0.0	25.577038	10-11
GTATAGG	540	0.0	25.390343	1
TATCACG	165	1.1039521E-5	25.261106	2
GAGTACT	5805	0.0	24.44057	12-13
>>END_MODULE