FastQCFastQC Report
Mon 15 Aug 2016
SRR2921521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830303
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.066.428981
GTATCAA35900.055.8739781
TCAACGC44650.043.5734864
CAACGCA45950.042.5996865
AACGCAG49100.040.106876
ATCAACG49450.039.1000633
GTACATG89450.037.7291341
TATCAAC52550.037.585972
TACATGG93100.036.040422
ACATGGG94700.034.9289253
ACGCAGA59400.032.749897
CATGGGG78050.031.1016394
CGCAGAG62550.030.8136048
GCAGAGT68650.026.9498589
ATGGGGG41950.026.2383065
CAGAGTA65950.024.40029510-11
AGAGTAC61050.023.87387810-11
ATGGGGA33000.022.536795
GTATAGA5950.022.0079631
GTATAGG3901.2732926E-1121.3667071
Produced by FastQC (version 0.11.5)