##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921521_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1830303
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.807291470319395	31.0	30.0	34.0	25.0	34.0
2	29.92566094247783	31.0	30.0	34.0	25.0	34.0
3	30.012807169086212	31.0	30.0	34.0	25.0	34.0
4	33.394896364153915	37.0	35.0	37.0	27.0	37.0
5	33.42515091763495	37.0	35.0	37.0	27.0	37.0
6	33.424058748742695	37.0	35.0	37.0	27.0	37.0
7	33.26582702426866	37.0	35.0	37.0	26.0	37.0
8	33.21543536780522	37.0	35.0	37.0	26.0	37.0
9	34.69753641883339	39.0	35.0	39.0	27.0	39.0
10-11	34.576104612187166	39.0	35.0	39.0	26.0	39.0
12-13	34.43267590120324	39.0	35.0	39.0	25.0	39.0
14-15	35.58268303117025	40.0	36.0	41.0	25.0	41.0
16-17	35.49534011581689	40.0	36.0	41.0	25.0	41.0
18-19	35.500348029807085	40.0	36.0	41.0	25.0	41.0
20-21	35.51763259962968	40.0	36.0	41.0	25.0	41.0
22-23	35.46875899782714	40.0	36.0	41.0	25.0	41.0
24-25	35.422593144413796	40.0	35.0	41.0	25.0	41.0
26-27	35.333843904533836	40.0	35.0	41.0	24.0	41.0
28-29	35.2771560228006	40.0	35.0	41.0	24.0	41.0
30-31	35.18011471324694	40.0	35.0	41.0	24.0	41.0
32-33	35.06759181403298	40.0	35.0	41.0	24.0	41.0
34-35	34.9057773494334	40.0	35.0	41.0	23.0	41.0
36-37	34.76686155243148	39.0	34.5	41.0	22.5	41.0
38-39	34.64249143447834	39.0	34.0	41.0	21.5	41.0
40-41	34.49177240052603	39.0	34.0	41.0	20.5	41.0
42-43	34.297470965189916	39.0	34.0	41.0	18.5	41.0
44-45	34.10750077992551	39.0	33.0	41.0	17.5	41.0
46-47	33.86233454242276	38.5	33.0	41.0	15.0	41.0
48-49	33.714582776731504	38.0	33.0	41.0	14.5	41.0
50-51	33.51590802178656	38.0	33.0	40.0	12.5	41.0
52-53	33.3212749473721	38.0	33.0	40.0	9.5	41.0
54-55	33.110955399188	38.0	33.0	40.0	8.5	41.0
56-57	32.86345648780557	37.0	32.0	40.0	8.0	41.0
58-59	32.61300478663915	37.0	32.0	40.0	7.0	41.0
60-61	32.29771873837283	36.0	32.0	40.0	4.0	41.0
62-63	31.981040297699344	36.0	31.0	39.0	2.0	41.0
64-65	31.668998247831098	35.0	31.0	39.0	2.0	41.0
66-67	31.62464165769274	35.0	31.0	39.0	2.0	41.0
68-69	31.377445428434527	35.0	31.0	38.5	2.0	40.0
70-71	31.081943536124893	35.0	31.0	37.0	2.0	40.0
72-73	30.76081091491409	35.0	31.0	37.0	2.0	39.0
74-75	30.465141290813598	35.0	31.0	36.0	2.0	39.0
76-77	30.194965533029233	35.0	31.0	36.0	2.0	38.5
78-79	29.925080437501332	35.0	30.0	35.5	2.0	37.0
80-81	29.692311600866084	35.0	30.0	35.0	2.0	37.0
82-83	29.500060645696365	35.0	30.0	35.0	2.0	36.5
84-85	29.31454628004216	35.0	30.0	35.0	2.0	36.0
86-87	29.164520300737088	34.5	30.0	35.0	2.0	36.0
88-89	29.03786886652101	34.0	30.0	35.0	2.0	35.5
90-91	28.929746877975944	34.0	30.0	35.0	2.0	35.0
92-93	28.828825882927582	34.0	29.5	35.0	2.0	35.0
94-95	28.766208928248492	34.0	29.0	35.0	2.0	35.0
96-97	28.66793339682009	34.0	29.0	35.0	2.0	35.0
98-99	28.607277866014535	34.0	29.0	35.0	2.0	35.0
100-101	28.544091060332633	34.0	29.0	35.0	2.0	35.0
102-103	27.568861275974527	32.5	27.5	34.5	2.0	35.0
104-105	28.383015271241973	34.0	29.0	35.0	2.0	35.0
106-107	28.701301096048034	34.5	30.0	35.0	2.0	35.0
108-109	28.738954697664813	35.0	30.0	35.0	2.0	35.0
110-111	28.678996046009868	35.0	30.0	35.0	2.0	35.0
112-113	28.60461956299039	35.0	30.0	35.0	2.0	35.0
114-115	28.481942060959305	35.0	30.0	35.0	2.0	35.0
116-117	28.418288119508084	35.0	30.0	35.0	2.0	35.0
118-119	28.25879458209925	34.5	29.0	35.0	2.0	35.0
120-121	28.08063856093772	34.5	29.0	35.0	2.0	35.0
122-123	27.9538218535401	34.0	29.0	35.0	2.0	35.0
124-125	27.10168589572328	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73788.0
3	13052.0
4	8556.0
5	7353.0
6	7578.0
7	8813.0
8	9604.0
9	10019.0
10	10428.0
11	10840.0
12	11111.0
13	11381.0
14	12127.0
15	10475.0
16	7918.0
17	7121.0
18	6656.0
19	6031.0
20	6039.0
21	6241.0
22	7504.0
23	9977.0
24	14122.0
25	18890.0
26	22670.0
27	24739.0
28	29215.0
29	34372.0
30	39545.0
31	46998.0
32	58984.0
33	76447.0
34	106085.0
35	167057.0
36	321293.0
37	473573.0
38	133562.0
39	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.59591748394234	16.581368300926513	11.72914471354178	25.093569501589368
2	17.075920671216647	18.954481263034552	38.08434148452905	25.88525658121975
3	19.843433360804998	23.40902979837837	28.182322966240452	28.565213874576173
4	13.532957630393353	14.54703856557339	35.006561410815365	36.913442393217885
5	14.610087932172855	36.81941110367776	33.18122291745919	15.38927804669019
6	34.487480566506825	33.779128373665344	16.947536674910186	14.785854384917641
7	30.635295017942404	30.70930572599093	19.941950242283944	18.713449013782718
8	28.150317073197396	33.35406707456385	19.016685013659128	19.478930838579625
9	27.74761746263118	14.55964391334598	18.453357906110785	39.239380717912056
10-11	26.807723316161297	24.456976537625884	26.623877782484524	22.111422363728288
12-13	26.919913004646567	22.081274736639003	26.75713833605809	24.241673922656343
14-15	24.26580627670727	23.40423206042115	23.943979912088057	28.38598175078352
16-17	24.655240408647284	26.419821377376707	24.444527041412144	24.480411172563862
18-19	24.647200509942458	25.57076052931677	25.969453747103966	23.812585213636805
20-21	25.593364671083474	24.606430571939	25.727618545131964	24.07258621184556
22-23	25.402337967993372	24.22138121343081	25.601747257268055	24.774533561307763
24-25	24.89186294273862	24.759353698257286	25.601540573745616	24.74724278525848
26-27	24.580788599592843	24.76772129616874	25.756313454343488	24.895176649894932
28-29	24.60426164419095	24.829816038688644	25.787848215996483	24.778074101123917
30-31	24.967240941033694	24.777410599339078	25.59377673546479	24.66157172416244
32-33	24.531605362141356	24.879258878586423	25.582602562981105	25.006533196291116
34-35	24.878720314149234	24.87273490542781	25.68694180597546	24.561602974447496
36-37	24.958636843099356	24.712740289669203	25.50222785666822	24.82639501056322
38-39	24.890323540089316	24.870804848071312	25.47995752907574	24.758914082763628
40-41	24.906166703474756	24.756219291666575	25.717429760324666	24.620184244534
42-43	24.822295658801107	24.80756156813369	25.527591156593317	24.842551616471894
44-45	24.768173649568112	24.817953236165184	25.54668701952858	24.867186094738123
46-47	24.87531790755523	24.881058901780612	25.37945921474681	24.86416397591735
48-49	24.978720798840616	25.053286837071564	25.1410550001066	24.826937363981212
50-51	24.915893940536986	25.192402519519057	25.0737192207903	24.81798431915366
52-53	24.99411580586123	25.05288940585358	25.232053207876753	24.720941580408436
54-55	24.862269570075515	25.0147050945226	25.326245368441885	24.796779966960003
56-57	24.819800367341585	25.121217256308213	25.094295272663665	24.964687103686536
58-59	24.904351010880944	24.956172837819	25.180925413194338	24.958550738105714
60-61	24.927962317405246	25.08132934119954	25.356007166601625	24.634701174793587
62-63	24.749481525697412	25.18932374770827	25.24472424382559	24.816470482768725
64-65	24.80078429057452	24.851046012296766	25.350930128827436	24.99723956830128
66-67	24.748338792802354	25.077819980180845	25.258930782168004	24.914910444848797
68-69	24.762084344461204	25.07750158501126	25.24603200629632	24.914382064231212
70-71	24.68121628584275	24.88187146970834	25.444832124509535	24.992080119939374
72-73	24.906149390876276	25.139911670979455	25.148574593915242	24.80536434422903
74-75	24.631961171066553	25.2899147043623	25.13383043798978	24.94429368658137
76-77	24.76158879587585	24.944250474964093	25.269428709475015	25.024732019685047
78-79	24.737510644414783	24.9952175286593	25.26863824730897	24.998633579616943
80-81	24.682823631384892	25.269943875720475	25.127085054283892	24.92014743861074
82-83	24.842119873865304	25.14920501861118	25.095760891631652	24.912914215891867
84-85	24.66254423381741	25.180931375455522	25.04491349694643	25.11161089378064
86-87	24.504399493640864	25.261943580794092	25.2568067624574	24.976850163107645
88-89	24.631816990699413	25.06161089783999	25.178506971000864	25.128065140459732
90-91	24.580748243486774	25.121581643302953	25.20428448246656	25.093385630743708
92-93	24.63154949497047	25.226529638618217	25.201254936764062	24.940665929647253
94-95	24.62412871386951	24.97481531917126	25.280829341717386	25.120226625241838
96-97	24.61079711014071	25.04805184763623	25.23181686665616	25.109334175566904
98-99	24.53227485848099	25.034254444334852	25.13069965949101	25.30277103769314
100-101	24.498020825592224	25.113056649632583	25.230949216854565	25.157973307920635
102-103	24.5610047353434	24.899305994759512	25.406525665462866	25.133163604434223
104-105	24.361779204727878	25.082806696223187	25.289973696375085	25.265440402673857
106-107	24.412942623817464	25.07825291571853	25.29766433795315	25.211140122510855
108-109	24.427173908588806	25.02243818740429	25.233363979503043	25.317023924503868
110-111	24.446758741952245	25.143661596278815	25.292472990199265	25.117106671569672
112-113	24.432892210793536	25.087896209118256	25.36178213542505	25.11742944466316
114-115	24.21558636705143	25.24800218564306	25.377364934089204	25.15904651321631
116-117	24.242127032956226	25.27441664777027	25.280618308285362	25.20283801098814
118-119	24.380657305896936	24.962086089360174	25.277063193137213	25.380193411605685
120-121	24.041428020363632	25.052316899078075	25.494265497604786	25.411989582953503
122-123	24.026668881877697	25.141916510955188	25.419546577635586	25.411868029531533
124-125	24.048798385116143	25.192210026693445	25.411172235974327	25.347819352216085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	157.5
2	182.0
3	194.5
4	178.5
5	162.0
6	157.0
7	206.0
8	265.0
9	301.0
10	305.5
11	326.0
12	378.0
13	447.0
14	517.5
15	569.5
16	646.0
17	729.5
18	799.0
19	899.0
20	986.0
21	1087.5
22	1319.0
23	1610.5
24	1895.0
25	2426.5
26	3334.0
27	4379.0
28	5359.0
29	6777.5
30	8718.5
31	10776.0
32	13552.5
33	16312.5
34	18925.0
35	21726.0
36	25302.0
37	29212.5
38	31930.0
39	34855.5
40	37657.0
41	39948.5
42	42875.0
43	48107.5
44	53631.5
45	57550.5
46	61670.5
47	65476.5
48	70349.5
49	75782.5
50	76823.0
51	76867.5
52	77327.5
53	77360.0
54	78854.5
55	78572.5
56	74867.0
57	68952.5
58	64757.5
59	60638.0
60	53986.5
61	47889.0
62	41653.5
63	33179.5
64	25116.0
65	19675.0
66	15394.0
67	11867.5
68	9434.0
69	7496.0
70	6416.0
71	5313.0
72	4137.5
73	3680.5
74	2966.5
75	2221.5
76	1514.5
77	860.0
78	528.5
79	323.0
80	190.5
81	100.0
82	58.0
83	29.0
84	16.5
85	18.0
86	15.5
87	12.5
88	14.5
89	11.0
90	8.5
91	8.0
92	8.5
93	9.5
94	11.5
95	12.0
96	7.0
97	4.5
98	7.0
99	12.0
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03534933833359832
2	0.030104305134177237
3	0.0294486759842496
4	0.03638741782098374
5	0.03884602713321237
6	0.05392549758154797
7	0.04578476896994651
8	0.04135927220793497
9	0.03474834494616465
10-11	0.04250662322030833
12-13	0.04261589474529627
14-15	0.04070364305800733
16-17	0.043899835163904555
18-19	0.05884271620600523
20-21	0.07132698793587729
22-23	0.062858444749312
24-25	0.0750695376667142
26-27	0.056548014181258516
28-29	0.05641142477502359
30-31	0.05671192146874042
32-33	0.06463410703036601
34-35	0.04665894116985002
36-37	0.05892466984974619
38-39	0.07031622632973884
40-41	0.05436258368149974
42-43	0.06619122626144415
44-45	0.06774834549252227
46-47	0.07384023301059989
48-49	0.057476822143656
50-51	0.0581870870560776
52-53	0.06832202099870896
54-55	0.05490894130643942
56-57	0.05152152403181331
58-59	0.05234106046922286
60-61	0.038518212558248555
62-63	0.049008278957090715
64-65	0.04802483523219926
66-67	0.04152317949541688
68-69	0.036223510533501826
70-71	0.057121689687445194
72-73	0.03641473570223072
74-75	0.046030629901169365
76-77	0.03775331188333298
78-79	0.038518212558248555
80-81	0.02865645742808704
82-83	0.019368377804112217
84-85	0.021663079828858938
86-87	0.020570364578979546
88-89	0.02581539777840063
90-91	0.014041390960950183
92-93	0.022400662622527524
94-95	0.01756539764181122
96-97	0.013576986979751439
98-99	0.013576986979751439
100-101	0.013495033336010485
102-103	0.0070753312429690605
104-105	0.007485099461673832
106-107	0.00980711936766754
108-109	0.015052152567088618
110-111	0.007321192174191923
112-113	0.008823675642776087
114-115	0.009725165723926586
116-117	0.008086092849107497
118-119	0.24345695767312844
120-121	0.35849255560418136
122-123	0.028956954121803876
124-125	0.004288907355776612
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1830303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.58355835381176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.02351781556291	46.080389078097625
2	10.254138005877556	11.809395084589784
3	3.508037742542359	6.06015888165186
4	1.7456845120090314	4.020909038584699
5	1.0441312398464286	3.0062396089367316
6	0.6727200088578591	2.3242567131505973
7	0.49165740767224436	1.981796811734495
8	0.34016570202230895	1.5670361241893576
9	0.2696816927673703	1.397630834318214
>10	1.5429914327458372	15.716994411789178
>50	0.08063069363581546	3.144530895643339
>100	0.025975441754004222	2.546951109052654
>500	3.8188840355792984E-4	0.14277327691221153
>1k	2.864163026684474E-4	0.2009381313493004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.185430499758783E-4
2	0.0	0.0	0.0	0.0	2.185430499758783E-4
3	0.0	0.0	0.0	0.0	2.185430499758783E-4
4	0.0	0.0	0.0	0.0	2.185430499758783E-4
5	0.0	0.0	0.0	0.0	2.185430499758783E-4
6	0.0	0.0	0.0	0.0	2.185430499758783E-4
7	0.0	0.0	0.0	0.0	2.185430499758783E-4
8	0.0	0.0	0.0	0.0	2.185430499758783E-4
9	0.0	0.0	0.0	0.0	2.185430499758783E-4
10-11	0.0	0.0	0.0	0.0	2.185430499758783E-4
12-13	0.0	0.0	0.0	0.0	3.2781457496381744E-4
14-15	0.0	0.0	0.0	0.0	3.8245033745778703E-4
16-17	5.4635762493969576E-5	0.0	0.0	0.0	4.6440398119874146E-4
18-19	5.4635762493969576E-5	0.0	0.0	0.0	4.917218624457262E-4
20-21	5.4635762493969576E-5	0.0	0.0	0.0	5.463576249396957E-4
22-23	5.4635762493969576E-5	0.0	0.0	0.0	5.736755061866805E-4
24-25	5.4635762493969576E-5	0.0	0.0	5.4635762493969576E-5	7.102649124216045E-4
26-27	5.4635762493969576E-5	0.0	0.0	1.0927152498793915E-4	7.375827936685893E-4
28-29	5.4635762493969576E-5	0.0	0.0	9.834437248914523E-4	7.649006749155741E-4
30-31	5.4635762493969576E-5	0.0	0.0	0.0034420530371200835	7.649006749155741E-4
32-33	5.4635762493969576E-5	0.0	0.0	0.009370033267715783	8.195364374095436E-4
34-35	5.4635762493969576E-5	0.0	0.0	0.0168824506106366	8.195364374095436E-4
36-37	5.4635762493969576E-5	0.0	0.0	0.028847682596815936	8.741721999035132E-4
38-39	5.4635762493969576E-5	0.0	0.0	0.04698675574481384	9.288079623974828E-4
40-41	5.4635762493969576E-5	0.0	0.0	0.06703808058010068	9.288079623974828E-4
42-43	5.4635762493969576E-5	0.0	0.0	0.08602400804675511	9.288079623974828E-4
44-45	5.4635762493969576E-5	0.0	0.0	0.10719536601316831	9.561258436444676E-4
46-47	5.4635762493969576E-5	0.0	0.0	0.13292881014782798	9.834437248914525E-4
48-49	5.4635762493969576E-5	0.0	0.0	0.16240480401332458	0.0010927152498793914
50-51	5.4635762493969576E-5	0.0	0.0	0.192181294572538	0.0010927152498793914
52-53	5.4635762493969576E-5	0.0	0.0	0.22332367919410062	0.0011200331311263761
54-55	5.4635762493969576E-5	0.0	0.0	0.25960182549009647	0.001147351012373361
56-57	5.4635762493969576E-5	0.0	0.0	0.29877566719827264	0.001174668893620346
58-59	5.4635762493969576E-5	0.0	0.0	0.3440140785432795	0.0012019867748673306
60-61	5.4635762493969576E-5	0.0	0.0	0.39318626478785207	0.0012019867748673306
62-63	5.4635762493969576E-5	0.0	0.0	0.44347848416355107	0.0013658940623492394
64-65	5.4635762493969576E-5	0.0	0.0	0.4941258359954609	0.0013932119435962243
66-67	5.4635762493969576E-5	0.0	0.0	0.5526680555077492	0.001420529824843209
68-69	5.4635762493969576E-5	0.0	0.0	0.6237765003936506	0.001420529824843209
70-71	1.0927152498793915E-4	0.0	0.0	0.7014412367788284	0.001420529824843209
72-73	1.0927152498793915E-4	0.0	0.0	0.7819197149324456	0.0016390728748190873
74-75	1.0927152498793915E-4	0.0	0.0	0.8633270010484603	0.0016390728748190873
76-77	1.0927152498793915E-4	0.0	0.0	0.953721870094733	0.0016937086373130569
78-79	1.0927152498793915E-4	0.0	0.0	1.0581308122207087	0.0017483443998070264
80-81	1.0927152498793915E-4	0.0	0.0	1.172565416764328	0.001830298043547981
82-83	1.0927152498793915E-4	0.0	0.0	1.295168067800796	0.0020215232122768744
84-85	1.0927152498793915E-4	0.0	0.0	1.420365917555727	0.0020215232122768744
86-87	1.0927152498793915E-4	0.0	0.0	1.5603700589465241	0.0020761589747708437
88-89	1.0927152498793915E-4	0.0	0.0	1.7161366178168316	0.0021307947372648135
90-91	1.0927152498793915E-4	0.0	0.0	1.8900422498351366	0.0021307947372648135
92-93	1.0927152498793915E-4	0.0	0.0	2.0762409284145846	0.002185430499758783
94-95	1.0927152498793915E-4	0.0	0.0	2.272082819074219	0.0024039735497346612
96-97	1.0927152498793915E-4	0.0	0.0	2.4770215641890987	0.0024859271934756155
98-99	1.0927152498793915E-4	0.0	0.0	2.70496196531394	0.00256788083721657
100-101	1.0927152498793915E-4	0.0	0.0	2.9594007112483562	0.00256788083721657
102-103	1.0927152498793915E-4	0.0	0.0	3.236130848280312	0.0026225165997105396
104-105	1.0927152498793915E-4	0.0	0.0	3.5279131378793567	0.0026225165997105396
106-107	1.0927152498793915E-4	0.0	0.0	3.8204876460345636	0.0027317881246984787
108-109	1.0927152498793915E-4	0.0	0.0	4.128879207431775	0.0028137417684394334
110-111	1.0927152498793915E-4	0.0	0.0	4.4705985839503075	0.002977649055921342
112-113	1.0927152498793915E-4	5.4635762493969576E-5	0.0	4.842367629840524	0.0030596026996622962
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2025	0.0	66.42898	1
GTATCAA	3590	0.0	55.873978	1
TCAACGC	4465	0.0	43.573486	4
CAACGCA	4595	0.0	42.599686	5
AACGCAG	4910	0.0	40.10687	6
ATCAACG	4945	0.0	39.100063	3
GTACATG	8945	0.0	37.729134	1
TATCAAC	5255	0.0	37.58597	2
TACATGG	9310	0.0	36.04042	2
ACATGGG	9470	0.0	34.928925	3
ACGCAGA	5940	0.0	32.74989	7
CATGGGG	7805	0.0	31.101639	4
CGCAGAG	6255	0.0	30.813604	8
GCAGAGT	6865	0.0	26.949858	9
ATGGGGG	4195	0.0	26.238306	5
CAGAGTA	6595	0.0	24.400295	10-11
AGAGTAC	6105	0.0	23.873878	10-11
ATGGGGA	3300	0.0	22.53679	5
GTATAGA	595	0.0	22.007963	1
GTATAGG	390	1.2732926E-11	21.366707	1
>>END_MODULE