FastQCFastQC Report
Mon 15 Aug 2016
SRR2921521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830303
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.13533278369756266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.12205629341152803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.077.883831
GTATCAA48900.055.706721
TCAACGC64600.041.663394
CAACGCA66900.040.2979855
AACGCAG70000.038.6728256
ATCAACG70200.038.424643
TATCAAC72150.037.470712
GTACATG92050.034.1908871
TACATGG93750.033.3472822
ACATGGG94550.032.6224753
ACGCAGA83650.032.2893077
CGCAGAG87450.030.8853828
CATGGGG77250.029.5222174
GCAGAGT95400.027.6879989
AGAGTAC84500.026.01445210-11
CAGAGTA90750.025.10782410-11
GTGGTAT11650.025.0709271
ATGGGGG42200.024.6795255
GAGTACT45650.023.39271412-13
AGTACTT50300.021.3484412-13
Produced by FastQC (version 0.11.5)