FastQCFastQC Report
Mon 15 Aug 2016
SRR2921520_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921520_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768591
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.072.554111
GTATCAA38700.059.6541861
TCAACGC48100.046.8762474
CAACGCA49400.045.5222365
ATCAACG52750.042.9671673
AACGCAG52100.042.8169176
TATCAAC55400.041.7709122
GTACATG96050.036.1772581
ACGCAGA61950.035.431927
TACATGG99050.034.5941122
ACATGGG100850.033.386793
CGCAGAG67000.032.670678
CATGGGG85750.031.4284384
TATACCG2109.276846E-1131.1624875
ATGGGGG48100.029.6841665
GCAGAGT72050.029.471829
CAGAGTA68950.025.36249210-11
GTATAGG4450.024.0675931
AGAGTAC66050.023.99955710-11
GTGGTAT7900.021.8419341
Produced by FastQC (version 0.11.5)