##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921520_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768591
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.929795526495386	31.0	30.0	34.0	26.0	34.0
2	29.962609218298635	31.0	30.0	34.0	25.0	34.0
3	30.12390089059596	31.0	31.0	34.0	26.0	34.0
4	33.51784782349339	37.0	35.0	37.0	27.0	37.0
5	33.54478904393385	37.0	35.0	37.0	27.0	37.0
6	33.53972286413309	37.0	35.0	37.0	28.0	37.0
7	33.419554323187214	37.0	35.0	37.0	27.0	37.0
8	33.33525331747136	37.0	35.0	37.0	26.0	37.0
9	34.819630428968594	39.0	35.0	39.0	27.0	39.0
10-11	34.70514324679929	39.0	35.0	39.0	27.0	39.0
12-13	34.55262437725851	39.0	35.0	39.0	25.5	39.0
14-15	35.70868052591017	40.0	36.0	41.0	25.5	41.0
16-17	35.63832451934902	40.0	36.0	41.0	25.0	41.0
18-19	35.64357983275953	40.0	36.0	41.0	25.0	41.0
20-21	35.664248263165426	40.0	36.0	41.0	25.0	41.0
22-23	35.63503772211891	40.0	36.0	41.0	25.0	41.0
24-25	35.587626534342874	40.0	35.5	41.0	25.0	41.0
26-27	35.51750432971784	40.0	35.0	41.0	25.0	41.0
28-29	35.4764467873013	40.0	35.0	41.0	25.0	41.0
30-31	35.382110957253545	40.0	35.0	41.0	25.0	41.0
32-33	35.277451655018034	40.0	35.0	41.0	25.0	41.0
34-35	35.11361332269587	40.0	35.0	41.0	24.0	41.0
36-37	34.97101591040551	39.0	35.0	41.0	24.0	41.0
38-39	34.83162981152793	39.0	34.5	41.0	23.5	41.0
40-41	34.678073958309184	39.0	34.0	41.0	23.0	41.0
42-43	34.47981981136397	39.0	34.0	41.0	22.0	41.0
44-45	34.26846851533226	39.0	33.5	41.0	20.5	41.0
46-47	34.01071757121912	38.0	33.0	41.0	19.0	41.0
48-49	33.861182432795374	38.0	33.0	40.0	18.5	41.0
50-51	33.65894601974114	38.0	33.0	40.0	16.0	41.0
52-53	33.44721249853697	38.0	33.0	40.0	15.0	41.0
54-55	33.235303979269375	37.5	33.0	40.0	14.0	41.0
56-57	32.98009262740792	37.0	32.5	40.0	13.0	41.0
58-59	32.72797554663571	36.5	32.0	40.0	11.0	41.0
60-61	32.41106677575539	36.0	32.0	39.5	8.0	41.0
62-63	32.094492734611904	35.5	31.5	39.0	7.5	41.0
64-65	31.787054497054434	35.0	31.0	39.0	7.0	40.5
66-67	31.74652279696097	35.0	31.0	39.0	5.5	40.5
68-69	31.499753476072193	35.0	31.0	37.5	2.0	40.0
70-71	31.20826691982488	35.0	31.0	37.0	2.0	39.5
72-73	30.893248071487413	35.0	31.0	37.0	2.0	39.0
74-75	30.599215420637105	35.0	31.0	36.0	2.0	39.0
76-77	30.334286728814067	35.0	31.0	36.0	2.0	38.0
78-79	30.07962864223554	35.0	31.0	35.0	2.0	37.0
80-81	29.842493827006923	35.0	30.0	35.0	2.0	37.0
82-83	29.658808339519993	35.0	30.0	35.0	2.0	36.0
84-85	29.470451053974603	35.0	30.0	35.0	2.0	36.0
86-87	29.328795917201887	34.0	30.0	35.0	2.0	36.0
88-89	29.214396375419753	34.0	30.0	35.0	2.0	35.0
90-91	29.105774596840085	34.0	30.0	35.0	2.0	35.0
92-93	29.01149983235242	34.0	30.0	35.0	2.0	35.0
94-95	28.95293145786674	34.0	30.0	35.0	2.0	35.0
96-97	28.855970939578455	34.0	29.5	35.0	2.0	35.0
98-99	28.792645671045484	34.0	29.0	35.0	2.0	35.0
100-101	28.72810248384166	34.0	29.0	35.0	2.0	35.0
102-103	27.722138131427783	32.5	28.0	34.5	2.0	35.0
104-105	28.564858127175814	34.0	29.0	35.0	2.0	35.0
106-107	28.906089932607372	34.5	30.0	35.0	2.0	35.0
108-109	28.948872294385758	35.0	30.0	35.0	2.0	35.0
110-111	28.890447254339755	35.0	30.0	35.0	2.0	35.0
112-113	28.816958528003365	35.0	30.0	35.0	2.0	35.0
114-115	28.69624661665699	35.0	30.0	35.0	2.0	35.0
116-117	28.63491502557686	35.0	30.0	35.0	2.0	35.0
118-119	28.474787556874368	34.5	30.0	35.0	2.0	35.0
120-121	28.305791729122223	34.0	29.5	35.0	2.0	35.0
122-123	28.183054476699247	34.0	29.5	35.0	2.0	35.0
124-125	27.305532200491804	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63954.0
3	11594.0
4	7567.0
5	6818.0
6	7240.0
7	8190.0
8	9168.0
9	9347.0
10	9966.0
11	10474.0
12	10452.0
13	10679.0
14	11695.0
15	10033.0
16	7608.0
17	6786.0
18	6252.0
19	5809.0
20	5632.0
21	5918.0
22	6935.0
23	9655.0
24	13786.0
25	18983.0
26	22205.0
27	23488.0
28	27962.0
29	33054.0
30	37829.0
31	46131.0
32	58230.0
33	76138.0
34	108519.0
35	174486.0
36	326559.0
37	448950.0
38	110388.0
39	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.07348055235981	16.60382651820328	12.109257015641598	25.21343591379531
2	17.017415068173953	19.044026323945605	37.87036429571682	26.068194312163627
3	19.53801177197857	23.54056366816979	28.375479399603076	28.54594516024856
4	13.812673108063548	14.732729916636641	34.613694350193974	36.840902625105834
5	14.930678105684214	36.77284409813664	32.672524185739235	15.623953610439914
6	34.38147356607153	33.424842428239934	17.05084270203183	15.142841303656706
7	30.51776742775884	30.748776230302884	20.072198735447408	18.66125760649087
8	28.21189535777534	32.97736892147874	19.17326511184881	19.63747060889711
9	28.11180402452861	14.552407217410288	18.39713403067094	38.938654727390166
10-11	26.963376327123473	24.433071064331745	26.186128316200204	22.417424292344577
12-13	27.101992311527646	22.01339218420659	26.423587710548503	24.461027793717264
14-15	24.495427840652315	23.205341906568105	23.737049507828978	28.5621807449506
16-17	24.901439520348426	26.163579286744536	24.236884527277354	24.698096665629684
18-19	25.06853491971543	25.2098192465741	25.676524230191344	24.045121603519128
20-21	26.12083955790171	24.25520876502206	25.30469857436286	24.319253102713372
22-23	25.846789294972105	23.816429794220245	25.242162050850354	25.094618859957297
24-25	25.372111687680505	24.347611087520896	25.17550036167997	25.104776863118627
26-27	24.99328234135436	24.396160271172786	25.329561261935513	25.280996125537342
28-29	24.890572288193912	24.620902807260993	25.29227629157228	25.196248612972816
30-31	25.350192901671058	24.418975641214207	25.224297690836888	25.006533766277844
32-33	24.96109098006602	24.40584186899074	25.168407742767673	25.46465940817556
34-35	25.205084425066442	24.586317033016293	25.329867391229953	24.87873115068731
36-37	25.291896371767276	24.44976847988731	25.176489889204117	25.081845259141293
38-39	25.446207063160642	24.415490953652853	25.063889349361162	25.074412633825343
40-41	25.382636321260645	24.42706376970317	25.119955694741748	25.070344214294444
42-43	25.356215619570488	24.431087517729452	25.081313623065743	25.131383239634314
44-45	25.10100144324146	24.48817543389179	25.13336263945158	25.27746048341517
46-47	25.16460294155089	24.540789335958607	25.068390068093027	25.226217654397477
48-49	25.47701195266453	24.679036251720436	24.66783540181352	25.176116393801518
50-51	25.346440835214317	24.887169130333938	24.68840690392546	25.07798313052628
52-53	25.417309755181293	24.69604166760961	24.82574375732668	25.060904819882413
54-55	25.22782588527458	24.596681926242088	25.059532143397583	25.11596004508575
56-57	25.126230057178283	24.684834970392643	24.710912521976418	25.478022450452652
58-59	25.336793807802245	24.59266546100659	24.719350278570833	25.35119045262033
60-61	25.300075283955124	24.662367581405373	24.994244821612092	25.043312313027403
62-63	25.128794724613336	24.757775411414826	24.896391485053023	25.217038378918815
64-65	25.14042592186153	24.476026056715668	24.99937777033616	25.38417025108664
66-67	25.069253291846877	24.727561297117337	24.974511677681676	25.228673733354114
68-69	25.306104442021148	24.692877472251507	24.805234544327455	25.19578354139989
70-71	25.027174858440414	24.516516628637422	25.033850129418227	25.422458383503933
72-73	25.32016575515284	24.794212992486834	24.81949537606697	25.066125876293356
74-75	25.065297486439192	24.89274525481363	24.819860957010142	25.222096301737036
76-77	25.106807664756143	24.542191254653172	24.84003949062635	25.51096158996434
78-79	25.0945199230662	24.70518466063749	24.89695355832973	25.303341857966583
80-81	25.090745384682382	24.856458843051488	24.795886510863994	25.256909261402132
82-83	25.20411963255497	24.84532150026198	24.706145488980862	25.244413378202186
84-85	25.013127285611	24.88390446333366	24.65512068761245	25.447847563442892
86-87	24.810204466532394	24.999483963129236	24.752210405056427	25.438101165281946
88-89	24.945141207662587	24.753474767217742	24.749317941200424	25.55206608391925
90-91	24.94644068410027	24.70585141711291	24.78753697268072	25.560170926106096
92-93	25.031938339070642	24.91388383210302	24.75089792116617	25.30327990766017
94-95	24.914686865665143	24.732481931485403	24.79615752300818	25.556673679841275
96-97	24.84962301846483	24.801641494037792	24.745347318943967	25.603388168553415
98-99	24.87431424534685	24.754771054148712	24.663049646332542	25.707865054171897
100-101	24.779545485390116	24.870164533655434	24.80960103868373	25.540688942270716
102-103	24.96189518950623	24.64456008063433	25.00339981707429	25.39014491278515
104-105	24.821972169783717	24.830991247536364	24.79850560384422	25.548530978835704
106-107	24.75832424572369	24.838677846806227	24.83036540531493	25.57263250215516
108-109	24.831156830888784	24.71101706273111	24.876142091218007	25.581684015162097
110-111	24.815498494047013	24.964694287161045	24.786716739756677	25.433090479035265
112-113	24.772802132944292	24.983269223429026	24.811960773503312	25.431967870123373
114-115	24.561675331563787	25.15273365535009	24.861883764090116	25.42370724899601
116-117	24.60987221077786	25.144518476255318	24.80108388150361	25.444525431463212
118-119	24.748738805678308	24.847866043905036	24.807937433653045	25.595457716763615
120-121	24.43033133835822	24.9322525515936	24.996794195280604	25.640621914767582
122-123	24.51017486826485	24.94399453434163	24.9280454898421	25.617785107551423
124-125	24.528436312397126	25.10962488076262	24.87069777120107	25.491241035639185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	172.5
2	203.5
3	214.0
4	187.5
5	155.0
6	159.0
7	183.0
8	245.5
9	304.0
10	310.0
11	348.5
12	404.0
13	452.0
14	558.0
15	611.0
16	659.5
17	735.0
18	822.0
19	918.5
20	1013.0
21	1193.0
22	1450.5
23	1740.5
24	2109.0
25	2655.5
26	3326.0
27	4084.0
28	5020.0
29	6173.5
30	7424.0
31	8754.5
32	10373.5
33	11963.5
34	13555.5
35	15571.5
36	18433.0
37	21633.0
38	25090.0
39	28712.0
40	31998.5
41	35414.0
42	39165.0
43	45128.5
44	51030.5
45	55310.5
46	60203.0
47	64610.5
48	70186.0
49	76018.5
50	76501.5
51	76011.5
52	76767.5
53	77780.0
54	79311.0
55	78297.5
56	73946.5
57	67972.0
58	64259.0
59	60447.5
60	54478.0
61	48972.0
62	43039.5
63	34109.0
64	26303.0
65	21527.5
66	16655.0
67	12765.0
68	10355.0
69	8353.5
70	7150.0
71	5943.5
72	4764.5
73	4399.0
74	3701.0
75	2814.5
76	1926.0
77	1097.0
78	631.5
79	446.5
80	287.0
81	136.5
82	82.5
83	50.0
84	30.5
85	18.0
86	16.5
87	13.5
88	13.0
89	11.5
90	8.0
91	8.5
92	7.0
93	8.0
94	9.0
95	7.0
96	7.5
97	7.0
98	9.5
99	12.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034660359574373044
2	0.026405200524032973
3	0.02793183952649312
4	0.030985117531413423
5	0.03432110646271524
6	0.04664730285294904
7	0.039183734396477196
8	0.03528232361241237
9	0.0301369847522689
10-11	0.03519751033449792
12-13	0.03502788377866901
14-15	0.03437764864799154
16-17	0.0362435407621095
18-19	0.04970058085786934
20-21	0.06024569841189964
22-23	0.055015546273841726
24-25	0.06471253104872748
26-27	0.04873936370817221
28-29	0.04927651446829708
30-31	0.04811739967013289
32-33	0.05688143838795968
34-35	0.0407951866768518
36-37	0.05235806356585554
38-39	0.061178644468958626
40-41	0.0483435684112381
42-43	0.05948237891066956
44-45	0.058916957057906554
46-47	0.06536276617940497
48-49	0.04907861681983002
50-51	0.05094450893394798
52-53	0.06100901791312971
54-55	0.04797604420694213
56-57	0.044498699812449576
58-59	0.046251507556014926
60-61	0.034943070500754556
62-63	0.04288724753207497
64-65	0.04268934988360791
66-67	0.03531059470505052
68-69	0.03205941905166316
70-71	0.04933305665357338
72-73	0.031748437032643496
74-75	0.0407386444915755
76-77	0.03259656981178803
78-79	0.03265311199706433
80-81	0.025839778671269954
82-83	0.018461023492712562
84-85	0.018517565677988863
86-87	0.017330179787186522
88-89	0.023465006889665276
90-91	0.01269372059452977
92-93	0.019224342993942635
94-95	0.01427690178226622
96-97	0.011421521425812979
98-99	0.01088437066568811
100-101	0.011251894869984071
102-103	0.006417538028860263
104-105	0.006643706769965469
106-107	0.008905394181017545
108-109	0.013230871354654637
110-111	0.0061913692877550545
112-113	0.008113803587149318
114-115	0.008650954347274186
116-117	0.007463568456471846
118-119	0.23702484067825744
120-121	0.3484694878578484
122-123	0.02600940522709886
124-125	0.003392531116578112
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1768591.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.906844775877865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51055771844146	44.67788121350948
2	11.151387159948325	12.691805162937936
3	3.909521733481345	6.674356393054315
4	1.8813469378377161	4.282460726444161
5	1.1247439473069256	3.200281461100169
6	0.7545042815494334	2.576187481972131
7	0.5046679111279103	2.010334093734576
8	0.35193881412580286	1.6022181972850875
9	0.28053444400965233	1.4367897053590035
>10	1.429032767192977	14.34612460485219
>50	0.07097641549004315	2.738744909177294
>100	0.028489177349999492	2.8332025846603517
>500	0.0020988058655126724	0.7701374085000323
>1k	1.9988627290596882E-4	0.15947605741334395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1308437055260374E-4
2	0.0	0.0	0.0	0.0	1.1308437055260374E-4
3	0.0	0.0	0.0	0.0	1.1308437055260374E-4
4	0.0	0.0	0.0	0.0	1.1308437055260374E-4
5	0.0	0.0	0.0	0.0	1.1308437055260374E-4
6	0.0	0.0	0.0	0.0	1.696265558289056E-4
7	0.0	0.0	0.0	0.0	1.696265558289056E-4
8	0.0	0.0	0.0	0.0	1.696265558289056E-4
9	0.0	0.0	0.0	0.0	2.2616874110520748E-4
10-11	0.0	0.0	0.0	0.0	2.2616874110520748E-4
12-13	0.0	0.0	0.0	0.0	3.6752420429596213E-4
14-15	0.0	0.0	0.0	0.0	5.936929454011696E-4
16-17	0.0	0.0	0.0	0.0	7.915905938682262E-4
18-19	0.0	0.0	0.0	0.0	7.915905938682262E-4
20-21	0.0	0.0	0.0	0.0	9.329460570589808E-4
22-23	0.0	0.0	0.0	0.0	0.0010460304276115845
24-25	0.0	0.0	0.0	5.654218527630187E-5	0.0010743015202497356
26-27	0.0	0.0	0.0	2.544398337433584E-4	0.0010743015202497356
28-29	0.0	0.0	0.0	0.0010460304276115845	0.0010743015202497356
30-31	0.0	0.0	0.0	0.0037600553208740743	0.0010743015202497356
32-33	0.0	0.0	0.0	0.00907502073684645	0.0010743015202497356
34-35	0.0	0.0	0.0	0.017697703991482485	0.0011308437055260374
36-37	0.0	0.0	0.0	0.03027834021545965	0.0011308437055260374
38-39	0.0	0.0	0.0	0.04899380354191557	0.0011308437055260374
40-41	0.0	0.0	0.0	0.07124315344814036	0.0011308437055260374
42-43	0.0	0.0	0.0	0.09329460570589808	0.0011591147981641883
44-45	0.0	0.0	0.0	0.11975634841520735	0.0012156569834404901
46-47	0.0	0.0	0.0	0.14862113399875948	0.0013852835392693958
48-49	0.0	0.0	0.0	0.18172658347803422	0.0015266390024601506
50-51	0.0	0.0	0.0	0.21621731649657833	0.0016397233730127542
52-53	0.0	0.0	0.0	0.25127347136788547	0.0019507053920324144
54-55	0.0	0.0	0.0	0.2916163205625269	0.002035518669946867
56-57	0.0	0.0	0.0	0.3367935265982921	0.002035518669946867
58-59	0.0	0.0	0.0	0.3867202761972667	0.002035518669946867
60-61	0.0	0.0	0.0	0.44111385843306905	0.0020920608552231694
62-63	0.0	0.0	0.0	0.5014443701228831	0.002148603040499471
64-65	0.0	0.0	0.0	0.5611811888672961	0.002233416318413924
66-67	0.0	0.0	0.0	0.6314913962583775	0.0022899585036902257
68-69	0.0	0.0	0.0	0.7131100407047192	0.0023747717816046785
70-71	0.0	0.0	0.0	0.8061219354842357	0.0023747717816046785
72-73	0.0	0.0	0.0	0.8965611608336805	0.002657482707986188
74-75	0.0	0.0	0.0	0.9929655867297753	0.0027705670785387916
76-77	0.0	0.0	0.0	1.0957310084694538	0.0028271092638150934
78-79	0.0	0.0	0.0	1.2195301231319169	0.0028271092638150934
80-81	0.0	0.0	0.0	1.349011727414648	0.0028271092638150934
82-83	0.0	0.0	0.0	1.494296872482106	0.003053278004920301
84-85	0.0	0.0	0.0	1.6435116994262664	0.003053278004920301
86-87	0.0	0.0	0.0	1.7989178956581822	0.0031663623754729048
88-89	0.0	0.0	0.0	1.9792309245043087	0.0032794467460255084
90-91	0.0	0.0	0.0	2.178711754159102	0.0032794467460255084
92-93	0.0	0.0	0.0	2.3916778950022928	0.0036186998576833197
94-95	0.0	0.0	0.0	2.6174225697179283	0.0037317842282359234
96-97	0.0	0.0	0.0	2.8522422651704096	0.003816597506150376
98-99	0.0	0.0	0.0	3.1080108402677613	0.003844868598788527
100-101	0.0	0.0	0.0	3.397591642160341	0.003844868598788527
102-103	0.0	0.0	0.0	3.714991199208862	0.003844868598788527
104-105	0.0	5.654218527630187E-5	0.0	4.038214601340841	0.003844868598788527
106-107	0.0	5.654218527630187E-5	0.0	4.390755126538584	0.003844868598788527
108-109	0.0	5.654218527630187E-5	0.0	4.754151751309376	0.003873139691426678
110-111	0.0	5.654218527630187E-5	0.0	5.1463000772931675	0.0040144951546174324
112-113	0.0	5.654218527630187E-5	5.654218527630187E-5	5.566945664656215	0.004071037339893734
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1960	0.0	72.55411	1
GTATCAA	3870	0.0	59.654186	1
TCAACGC	4810	0.0	46.876247	4
CAACGCA	4940	0.0	45.522236	5
ATCAACG	5275	0.0	42.967167	3
AACGCAG	5210	0.0	42.816917	6
TATCAAC	5540	0.0	41.770912	2
GTACATG	9605	0.0	36.177258	1
ACGCAGA	6195	0.0	35.43192	7
TACATGG	9905	0.0	34.594112	2
ACATGGG	10085	0.0	33.38679	3
CGCAGAG	6700	0.0	32.67067	8
CATGGGG	8575	0.0	31.428438	4
TATACCG	210	9.276846E-11	31.162487	5
ATGGGGG	4810	0.0	29.684166	5
GCAGAGT	7205	0.0	29.47182	9
CAGAGTA	6895	0.0	25.362492	10-11
GTATAGG	445	0.0	24.067593	1
AGAGTAC	6605	0.0	23.999557	10-11
GTGGTAT	790	0.0	21.841934	1
>>END_MODULE