FastQCFastQC Report
Mon 15 Aug 2016
SRR2921520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768591
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.17629853369150922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.15543446732455385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26950.080.497361
GTATCAA55600.063.4579351
TCAACGC73950.047.099264
CAACGCA75550.045.9969445
ATCAACG78950.044.2672273
AACGCAG79650.043.7675176
TATCAAC81250.042.867572
ACGCAGA94300.036.777717
CGCAGAG99350.034.788538
GTACATG93650.034.4929661
GCAGAGT100250.033.467449
TACATGG98000.032.928322
ACATGGG100100.031.7616863
CATGGGG86650.029.408414
AGAGTAC95300.027.59073810-11
CAGAGTA99150.027.2993710-11
ATGGGGG44200.027.1944145
TATAGCG1107.723812E-427.0628592
GAGTACT52950.025.72821212-13
GTGGTAT9250.024.4839171
Produced by FastQC (version 0.11.5)