##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768591
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07294168069384	31.0	31.0	34.0	28.0	34.0
2	31.144797751430374	33.0	31.0	34.0	27.0	34.0
3	31.219045556604097	33.0	31.0	34.0	27.0	34.0
4	34.737050567372556	37.0	35.0	37.0	32.0	37.0
5	34.378944029456214	37.0	35.0	37.0	30.0	37.0
6	34.350934161714044	37.0	35.0	37.0	30.0	37.0
7	34.19286426313376	37.0	35.0	37.0	30.0	37.0
8	34.1694422283049	37.0	35.0	37.0	30.0	37.0
9	35.71823389353446	39.0	37.0	39.0	30.0	39.0
10-11	35.57821423947085	39.0	35.0	39.0	30.0	39.0
12-13	35.475457299058974	39.0	35.0	39.0	30.0	39.0
14-15	36.61664257027204	40.0	36.0	41.0	29.0	41.0
16-17	36.583958360073076	40.0	36.0	41.0	29.0	41.0
18-19	36.58981810944418	40.0	36.5	41.0	29.0	41.0
20-21	36.565169391905755	40.0	36.5	41.0	29.0	41.0
22-23	36.495472101803074	40.0	36.0	41.0	29.0	41.0
24-25	36.412500402863074	40.0	36.0	41.0	28.5	41.0
26-27	36.27609549070418	40.0	36.0	41.0	28.0	41.0
28-29	36.07493648899039	40.0	36.0	41.0	27.0	41.0
30-31	36.02364170121865	40.0	35.5	41.0	27.5	41.0
32-33	35.890031386567046	40.0	35.0	41.0	27.0	41.0
34-35	35.753061052555395	40.0	35.0	41.0	27.0	41.0
36-37	35.608503039990595	40.0	35.0	41.0	26.5	41.0
38-39	35.41816112374201	39.0	35.0	41.0	25.5	41.0
40-41	35.20139987142307	39.0	35.0	41.0	25.0	41.0
42-43	35.012170705380726	39.0	35.0	41.0	24.0	41.0
44-45	34.77455104091336	39.0	34.0	41.0	23.5	41.0
46-47	34.477885503205655	39.0	34.0	41.0	22.5	41.0
48-49	34.200222097703765	39.0	34.0	41.0	20.5	41.0
50-51	33.95826112425088	38.0	33.0	40.5	19.5	41.0
52-53	33.747861433197386	38.0	33.0	40.0	18.5	41.0
54-55	33.515346114505846	38.0	33.0	40.0	18.0	41.0
56-57	33.24591920913315	37.0	33.0	40.0	16.0	41.0
58-59	33.09140496587396	37.0	33.0	40.0	16.0	41.0
60-61	32.96435806808923	36.5	33.0	40.0	15.0	41.0
62-63	32.69812834058298	36.0	33.0	39.5	13.0	41.0
64-65	32.39868601615636	35.0	32.0	39.0	12.5	41.0
66-67	32.09800343889571	35.0	32.0	39.0	10.5	41.0
68-69	31.78632199304418	35.0	32.0	38.0	8.0	40.0
70-71	31.494977640392833	35.0	32.0	37.0	7.5	40.0
72-73	31.193705328139746	35.0	31.5	37.0	7.0	39.0
74-75	30.91706222637116	35.0	31.0	36.0	5.5	39.0
76-77	30.64504908144393	35.0	31.0	36.0	2.0	38.0
78-79	30.381283179661096	35.0	31.0	35.0	2.0	37.0
80-81	30.176240860662528	35.0	31.0	35.0	2.0	37.0
82-83	30.009568068592458	35.0	31.0	35.0	2.0	36.0
84-85	29.83613254845241	35.0	31.0	35.0	2.0	36.0
86-87	29.70105779120215	35.0	31.0	35.0	2.0	36.0
88-89	29.609186352299655	35.0	30.5	35.0	2.0	35.0
90-91	29.512996503996682	35.0	30.0	35.0	2.0	35.0
92-93	29.583286073490143	35.0	31.0	35.0	2.0	35.0
94-95	29.55475969288547	35.0	31.0	35.0	2.0	35.0
96-97	29.544363281278713	35.0	31.0	35.0	2.0	35.0
98-99	29.504178750202847	35.0	31.0	35.0	2.0	35.0
100-101	29.454311087187484	35.0	31.0	35.0	2.0	35.0
102-103	28.568530824820435	33.5	29.0	35.0	2.0	35.0
104-105	29.27780956705083	34.5	30.5	35.0	2.0	35.0
106-107	29.390522455446174	35.0	31.0	35.0	2.0	35.0
108-109	29.395868519064045	35.0	31.0	35.0	2.0	35.0
110-111	29.362506368063617	35.0	31.0	35.0	2.0	35.0
112-113	29.311329470748184	35.0	31.0	35.0	2.0	35.0
114-115	29.24307428908097	35.0	31.0	35.0	2.0	35.0
116-117	29.163127597053247	35.0	31.0	35.0	2.0	35.0
118-119	29.07085357779159	35.0	31.0	35.0	2.0	35.0
120-121	28.947484466448152	35.0	31.0	35.0	2.0	35.0
122-123	28.8208949949423	35.0	31.0	35.0	2.0	35.0
124-125	27.895039045206044	34.0	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34403.0
3	12363.0
4	7116.0
5	6000.0
6	6325.0
7	7463.0
8	8382.0
9	9014.0
10	10095.0
11	11063.0
12	11439.0
13	13447.0
14	16614.0
15	14090.0
16	7701.0
17	6567.0
18	6015.0
19	5241.0
20	5104.0
21	5492.0
22	6607.0
23	8991.0
24	12483.0
25	16847.0
26	20929.0
27	23250.0
28	27241.0
29	32470.0
30	38063.0
31	45818.0
32	56988.0
33	73277.0
34	103035.0
35	165744.0
36	323456.0
37	476969.0
38	132228.0
39	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01744105083322	17.243657310806206	12.642525821925796	25.09637581643478
2	17.321473603978827	19.61457952845306	36.921578095650595	26.142368771917525
3	19.7511538302754	23.765199427001182	28.643606838660567	27.840039904062852
4	13.9836817915406	15.322166377384876	34.21641596835476	36.47773586271976
5	14.886697897066368	36.748939144834516	32.63576768699923	15.728595271099891
6	34.381982566875706	33.99227368636551	16.404476081624527	15.221267665134262
7	30.057630222191058	31.265936529281934	19.839759594151136	18.836673654375875
8	28.421955567970354	32.86210509590336	18.80696568106626	19.908973655060024
9	28.167832231098405	14.148828856540579	18.26468113595035	39.41865777641066
10-11	27.09898768856262	24.388633725902647	25.92523795185407	22.587140633680665
12-13	27.39630017901995	22.000002261780768	26.507872693407702	24.095824865791585
14-15	24.67124142021671	22.875558297291505	24.27786765067595	28.175332631815834
16-17	24.653786604750685	25.964732320073647	24.660685426183175	24.72079564899249
18-19	24.787701441899916	24.59406276505513	26.809923664122138	23.808312128922815
20-21	25.78307148483733	23.607927893693073	26.66678541881905	23.942215202650548
22-23	25.48651663981624	23.17543890402779	26.602048473258055	24.73599598289791
24-25	24.99076832946065	23.812875027779835	26.494696525442368	24.701660117317143
26-27	24.685165744427053	23.806731449157883	26.674302439366432	24.833800367048635
28-29	24.69903517648752	24.111398478076655	26.531699384936676	24.657866960499152
30-31	24.998225622241055	23.883209466213444	26.46220745035065	24.65635746119485
32-33	24.66891808214236	23.846832872550923	26.422200343932744	25.06204870137398
34-35	24.842278496403885	24.014556950392414	26.56844212636666	24.574722426837045
36-37	25.035831799637116	23.90906343069724	26.33920732622814	24.715897443437502
38-39	25.179388427975997	23.96107972379159	26.144138488771258	24.715393359461157
40-41	25.16669555311709	23.9228157814454	26.249075091670893	24.661413573766616
42-43	25.139480946816427	23.991627608985702	26.069627237470037	24.799264206727827
44-45	25.016958450030184	24.07639007686794	25.920061302075307	24.986590171026567
46-47	25.049489524846834	24.248382701272718	25.66638023393609	25.03574753994436
48-49	25.415683810542856	24.47160467156158	25.044606728419534	25.06810478947604
50-51	25.234189752936526	24.709316298815097	25.040956698717604	25.015537249530773
52-53	25.433175736050945	24.543269502663694	25.07366164843741	24.94989311284795
54-55	25.223980068919555	24.429796297956194	25.300098654437175	25.04612497868708
56-57	25.11532699593323	24.483877407558847	24.996140214553172	25.404655381954754
58-59	25.242838961568488	24.38257842341931	25.157757316837653	25.216825298174548
60-61	25.260928515582474	24.4639835091425	25.353336132707295	24.921751842567733
62-63	25.06042813773883	24.546972768032866	25.304204916141465	25.088394178086844
64-65	25.144745503529993	24.232585863292837	25.3456756750643	25.276992958112878
66-67	25.107640769924146	24.519702191042434	25.267136851653092	25.105520187380325
68-69	25.326896218633415	24.525282585206796	25.06846715852738	25.079354037632406
70-71	25.04615934460234	24.33596990608659	25.343925995755317	25.273944753555753
72-73	25.367603189315712	24.67416058212533	24.95598828013	25.002247948428952
74-75	25.10949326159424	24.751118260639913	24.934943089518253	25.2044453882476
76-77	25.15715783869342	24.428292680444173	24.985304225851497	25.429245255010912
78-79	25.14127640970286	24.54398890545743	25.047473907379008	25.267260777460702
80-81	25.091652205435537	24.688735961624978	24.947497035883554	25.272114797055934
82-83	25.17936102097312	24.69449605172496	24.87997125134406	25.24617167595786
84-85	24.98884561597364	24.6923001668775	24.797058070090813	25.52179614705804
86-87	24.82249189931971	24.762888988164242	24.888541425154237	25.52607768736181
88-89	24.909860807725206	24.587025215786905	24.843862886065928	25.659251090421957
90-91	24.876297952568535	24.52798126175523	24.928775895433176	25.66694489024306
92-93	24.95026001634768	24.710501561681397	24.828316830619414	25.510921591351504
94-95	24.81651465635038	24.541587042551864	24.898393550793045	25.743504750304712
96-97	24.827718402176707	24.57488323018348	24.885873601638863	25.71152476600095
98-99	24.75896398404694	24.488320605960716	24.85969545340046	25.89301995659189
100-101	24.757292259251678	24.62570304379936	24.96047234138325	25.65653235556571
102-103	24.95663079518879	24.311605757530522	25.097566574839302	25.634196872441382
104-105	24.784858761215066	24.562190158873314	24.932296135670658	25.720654944240966
106-107	24.772749054399153	24.573606745959832	24.81835049442082	25.83529370522019
108-109	24.840908236181672	24.382288495258422	24.891975950104072	25.884827318455834
110-111	24.79172532961509	24.5960879305295	24.844309948444458	25.767876791410956
112-113	24.769273136160656	24.559482886826924	24.861529953354182	25.80971402365823
114-115	24.461302587969737	24.733163947723234	24.947266077237913	25.858267387069112
116-117	24.50811943277771	24.718106469393266	24.845337069200724	25.9284370286283
118-119	24.500051317386585	24.407397281111553	25.02592022818283	26.06663117331903
120-121	24.27404192759503	24.534089142081676	25.07951541538853	26.11235351493476
122-123	24.306258999090513	24.50721100497772	25.0462022813198	26.140327714611967
124-125	24.353230039410345	24.617030800697094	24.952571569635737	26.077167590256817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	45.0
2	70.0
3	103.5
4	79.5
5	72.5
6	145.5
7	307.0
8	515.5
9	603.5
10	539.5
11	488.0
12	553.5
13	664.5
14	756.5
15	896.5
16	1042.5
17	1202.0
18	1441.0
19	1608.0
20	1792.5
21	2056.0
22	2310.0
23	2616.5
24	2960.5
25	3453.5
26	4064.0
27	4827.0
28	5648.0
29	6653.0
30	7913.0
31	9144.5
32	10633.5
33	12068.0
34	13561.0
35	15596.0
36	18461.0
37	21376.5
38	24560.5
39	27985.0
40	31069.0
41	34506.5
42	38202.0
43	44099.5
44	50229.0
45	54740.5
46	59703.0
47	64474.5
48	70399.0
49	76079.0
50	76265.0
51	75705.5
52	76788.0
53	78015.0
54	79468.0
55	77911.5
56	73318.0
57	67646.0
58	64250.5
59	60826.0
60	54728.0
61	48726.5
62	42688.5
63	33776.5
64	25784.5
65	21151.5
66	16527.5
67	12474.5
68	9918.5
69	8042.0
70	6904.5
71	5765.0
72	4721.5
73	4412.5
74	3605.0
75	2692.5
76	1789.0
77	980.5
78	583.0
79	379.0
80	209.5
81	85.0
82	55.0
83	28.0
84	13.5
85	6.5
86	2.5
87	1.5
88	0.5
89	1.5
90	2.0
91	0.5
92	0.5
93	0.5
94	0.0
95	1.0
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11698578133666857
2	0.0016397233730127544
3	0.019789764846705655
4	0.05625947434992036
5	0.02499164589212543
6	0.019676680476153053
7	0.014022461948522864
8	0.005201881045419772
9	0.0035621576724070175
10-11	0.006417538028860263
12-13	0.004127579525170036
14-15	0.002487856152157282
16-17	0.009781798052800224
18-19	0.00514533886014347
20-21	0.011421521425812979
22-23	0.008594412161997885
24-25	0.012552365131339015
26-27	0.00907502073684645
28-29	0.013372226817845391
30-31	0.020666168718488333
32-33	0.012552365131339015
34-35	0.020553084347935728
36-37	0.014672697079200336
38-39	0.020411728884744976
40-41	0.009753526960162073
42-43	0.009018478551570147
44-45	0.01687784230497611
46-47	0.01625587826693679
48-49	0.020355186699468675
50-51	0.014700968171838486
52-53	0.020977150737507996
54-55	0.016821300119699806
56-57	0.02024210232891607
58-59	0.016368962637489392
60-61	0.019139529716028184
62-63	0.0212033194786132
64-65	0.01781078836203509
66-67	0.011817316722747091
68-69	0.02304094050009301
70-71	0.014700968171838486
72-73	0.017641161806206184
74-75	0.01874373441909407
76-77	0.012750262779806072
78-79	0.02909095432465731
80-81	0.02021383123627792
82-83	0.00907502073684645
84-85	0.012891618242996826
86-87	0.012495822946062714
88-89	0.019987662495172708
90-91	0.012948160428273127
92-93	0.00817034577242562
94-95	0.0070960442521758845
96-97	0.002685753800624339
98-99	0.0015549100950983017
100-101	0.011817316722747091
102-103	0.0031098201901966034
104-105	0.0024595850595191316
106-107	3.675242042959622E-4
108-109	0.019818035939343805
110-111	7.350484085919244E-4
112-113	0.008933665273655696
114-115	0.0024030428742428294
116-117	0.008396514513530828
118-119	0.010432033183477695
120-121	0.003731784228235923
122-123	5.371507601248678E-4
124-125	0.022645145203158898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1768591.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.66132846517752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75196196746482	43.27777493883049
2	11.686820177106261	13.010078731827512
3	3.9808054776365327	6.647307637401148
4	1.941870593743587	4.3234838782092435
5	1.1295671842226807	3.143660503225215
6	0.7222188816270199	2.411979743639683
7	0.47929821863187066	1.8674862906030112
8	0.3982754378051592	1.773483197062828
9	0.27414223285279554	1.3733208782096968
>10	1.5248885700600667	14.897458730019292
>50	0.0762577898506611	2.8859584673008682
>100	0.03154577483191294	3.2127204361250543
>500	0.0019393995289762145	0.6789458804106789
>1k	4.082946376792031E-4	0.49634068713537166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3118	0.17629853369150922	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2749	0.15543446732455385	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.654218527630187E-5
2	0.0	0.0	0.0	0.0	5.654218527630187E-5
3	0.0	0.0	0.0	0.0	5.654218527630187E-5
4	0.0	0.0	0.0	1.696265558289056E-4	5.654218527630187E-5
5	0.0	0.0	0.0	1.696265558289056E-4	5.654218527630187E-5
6	0.0	0.0	0.0	1.696265558289056E-4	5.654218527630187E-5
7	0.0	0.0	0.0	1.696265558289056E-4	5.654218527630187E-5
8	0.0	0.0	0.0	1.696265558289056E-4	5.654218527630187E-5
9	0.0	0.0	0.0	1.696265558289056E-4	5.654218527630187E-5
10-11	0.0	0.0	0.0	1.696265558289056E-4	5.654218527630187E-5
12-13	0.0	0.0	0.0	1.696265558289056E-4	1.4135546319075468E-4
14-15	0.0	0.0	0.0	1.9789764846705655E-4	2.2616874110520748E-4
16-17	0.0	0.0	0.0	2.2616874110520748E-4	3.957952969341131E-4
18-19	0.0	0.0	0.0	2.2616874110520748E-4	4.5233748221041497E-4
20-21	0.0	0.0	0.0	2.2616874110520748E-4	4.5233748221041497E-4
22-23	0.0	0.0	0.0	2.2616874110520748E-4	4.806085748485659E-4
24-25	0.0	0.0	0.0	2.8271092638150935E-4	5.088796674867168E-4
26-27	0.0	0.0	0.0	4.806085748485659E-4	5.654218527630187E-4
28-29	0.0	0.0	0.0	0.001300470261354943	5.936929454011696E-4
30-31	0.0	0.0	0.0	0.004042766247255584	6.502351306774715E-4
32-33	0.0	0.0	0.0	0.009442544941142411	7.350484085919243E-4
34-35	0.0	0.0	0.0	0.018178312566331054	7.915905938682262E-4
36-37	0.0	0.0	0.0	0.030928575346137126	7.915905938682262E-4
38-39	0.0	0.0	0.0	0.0499550206916127	8.48132779144528E-4
40-41	0.0	0.0	0.0	0.0726001658947716	8.48132779144528E-4
42-43	0.0	0.0	0.0	0.09541493765375941	8.48132779144528E-4
44-45	0.0	0.0	0.0	0.12210284910417388	8.76403871782679E-4
46-47	0.0	0.0	0.0	0.15173095418895607	9.046749644208299E-4
48-49	0.0	0.0	0.0	0.18568453644737534	9.046749644208299E-4
50-51	0.0	0.0	0.0	0.22076896241132066	9.046749644208299E-4
52-53	0.0	0.0	0.0	0.2562209125795619	0.0010177593349734336
54-55	0.0	0.0	0.0	0.29752497892390045	0.0010743015202497356
56-57	0.0	0.0	0.0	0.3433524200903431	0.0010743015202497356
58-59	0.0	0.0	0.0	0.3942121157463766	0.0010743015202497356
60-61	0.0	0.0	0.0	0.4496234573171525	0.0011308437055260374
62-63	0.0	0.0	0.0	0.5105193908597296	0.0011308437055260374
64-65	0.0	0.0	0.0	0.5708781736421819	0.0011591147981641883
66-67	0.0	0.0	0.0	0.6419517005344932	0.0012156569834404901
68-69	0.0	0.0	0.0	0.7248142730569137	0.001243928076078641
70-71	0.0	0.0	0.0	0.8187025717082129	0.001243928076078641
72-73	0.0	0.0	0.0	0.9103574540410982	0.001357012446631245
74-75	0.0	0.0	0.0	1.0085146876807582	0.0014418257245456976
76-77	0.0	0.0	0.0	1.113004646071364	0.0014700968171838485
78-79	0.0	0.0	0.0	1.2379911466246294	0.0015266390024601506
80-81	0.0	0.0	0.0	1.3699323359668798	0.0015831811877364524
82-83	0.0	0.0	0.0	1.5170833731484556	0.001696265558289056
84-85	0.0	0.0	0.0	1.6683054476699248	0.001696265558289056
86-87	0.0	0.0	0.0	1.8259167891276165	0.001752807743565358
88-89	0.0	0.0	0.0	2.008350149921604	0.0018376210214798108
90-91	0.0	0.0	0.0	2.2115062216193566	0.0018658921141179617
92-93	0.0	0.0	0.0	2.428317231061336	0.0019224342993942635
94-95	0.0	0.0	0.0	2.658613551691714	0.0019224342993942635
96-97	0.0	0.0	0.0	2.897249844650346	0.002092060855223169
98-99	0.0	0.0	0.0	3.156524035234828	0.002261687411052075
100-101	0.0	0.0	0.0	3.4507978385053413	0.002261687411052075
102-103	0.0	0.0	0.0	3.772381517264308	0.0022899585036902257
104-105	0.0	0.0	0.0	4.099506330180352	0.0023182295963283766
106-107	0.0	0.0	0.0	4.454365084974423	0.0023465006889665276
108-109	0.0	0.0	0.0	4.823359386087569	0.0024313139668809803
110-111	0.0	0.0	0.0	5.222632027416175	0.0024313139668809803
112-113	0.0	0.0	0.0	5.651702400385391	0.0024313139668809803
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2695	0.0	80.49736	1
GTATCAA	5560	0.0	63.457935	1
TCAACGC	7395	0.0	47.09926	4
CAACGCA	7555	0.0	45.996944	5
ATCAACG	7895	0.0	44.267227	3
AACGCAG	7965	0.0	43.767517	6
TATCAAC	8125	0.0	42.86757	2
ACGCAGA	9430	0.0	36.77771	7
CGCAGAG	9935	0.0	34.78853	8
GTACATG	9365	0.0	34.492966	1
GCAGAGT	10025	0.0	33.46744	9
TACATGG	9800	0.0	32.92832	2
ACATGGG	10010	0.0	31.761686	3
CATGGGG	8665	0.0	29.40841	4
AGAGTAC	9530	0.0	27.590738	10-11
CAGAGTA	9915	0.0	27.29937	10-11
ATGGGGG	4420	0.0	27.194414	5
TATAGCG	110	7.723812E-4	27.062859	2
GAGTACT	5295	0.0	25.728212	12-13
GTGGTAT	925	0.0	24.483917	1
>>END_MODULE