FastQCFastQC Report
Mon 15 Aug 2016
SRR2921519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715541
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23010.1341267856611996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18690.10894522485909693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.071.857851
GTATCAA40750.060.451971
TCAACGC54800.044.621934
CAACGCA56100.043.6926845
AACGCAG58600.041.829886
ATCAACG59500.041.195963
TATCAAC61450.040.1767962
GTACATG96400.036.973241
ACGCAGA69950.034.871497
TACATGG100400.034.66342
ACATGGG100750.034.0135353
CGCAGAG74400.032.6210758
CATGGGG87750.030.3751164
GCAGAGT79600.029.8192149
GTATAGG5250.029.4680771
AGAGTAC74700.026.40010510-11
CAGAGTA78400.024.85066210-11
CATGGGT17450.024.5484524
ATGGGGG48800.024.5047685
ATGGGGA32050.023.946175