##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921519_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1715541 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.795129932773392 31.0 30.0 34.0 25.0 34.0 2 29.866489929415852 31.0 30.0 34.0 25.0 34.0 3 29.982941241276077 31.0 30.0 34.0 25.0 34.0 4 33.344217363502246 37.0 35.0 37.0 27.0 37.0 5 33.39129755569817 37.0 35.0 37.0 27.0 37.0 6 33.39627907464759 37.0 35.0 37.0 27.0 37.0 7 33.24060573311859 37.0 35.0 37.0 26.0 37.0 8 33.160661855356416 37.0 35.0 37.0 26.0 37.0 9 34.60906209761236 39.0 35.0 39.0 26.0 39.0 10-11 34.48553867264029 39.0 35.0 39.0 25.0 39.0 12-13 34.323385159550256 39.0 35.0 39.0 24.5 39.0 14-15 35.45521033889601 40.0 36.0 41.0 24.5 41.0 16-17 35.381133997963325 40.0 36.0 41.0 24.0 41.0 18-19 35.40667696079546 40.0 36.0 41.0 24.0 41.0 20-21 35.4360344637639 40.0 36.0 41.0 24.0 41.0 22-23 35.403142798685664 40.0 35.0 41.0 24.0 41.0 24-25 35.369501515848356 40.0 35.0 41.0 24.0 41.0 26-27 35.28850170296134 40.0 35.0 41.0 24.0 41.0 28-29 35.23739129522407 40.0 35.0 41.0 24.0 41.0 30-31 35.13882326333209 40.0 35.0 41.0 24.0 41.0 32-33 35.01969641063664 40.0 35.0 41.0 24.0 41.0 34-35 34.848508721155596 39.5 34.5 41.0 23.0 41.0 36-37 34.703862804794525 39.0 34.0 41.0 22.5 41.0 38-39 34.571783769667995 39.0 34.0 41.0 21.5 41.0 40-41 34.414091531476075 39.0 34.0 41.0 20.5 41.0 42-43 34.216508086953326 39.0 33.5 41.0 18.5 41.0 44-45 34.019607517395386 39.0 33.0 41.0 16.5 41.0 46-47 33.75409331516997 38.5 33.0 41.0 15.0 41.0 48-49 33.589693571881995 38.0 33.0 41.0 12.0 41.0 50-51 33.391175145333165 38.0 33.0 40.0 9.0 41.0 52-53 33.19530923481281 38.0 33.0 40.0 8.0 41.0 54-55 32.99345687453695 38.0 32.5 40.0 7.5 41.0 56-57 32.75319884514565 37.0 32.0 40.0 6.5 41.0 58-59 32.502994973597254 37.0 32.0 40.0 2.0 41.0 60-61 32.19677874209943 36.0 31.5 40.0 2.0 41.0 62-63 31.880283245926506 36.0 31.0 39.0 2.0 41.0 64-65 31.581742727221325 35.0 31.0 39.0 2.0 41.0 66-67 31.53715708339235 35.0 31.0 39.0 2.0 41.0 68-69 31.287126043621225 35.0 31.0 38.5 2.0 40.5 70-71 30.989245375074102 35.0 31.0 37.0 2.0 40.0 72-73 30.668266745009298 35.0 31.0 37.0 2.0 39.0 74-75 30.372297426875836 35.0 31.0 36.0 2.0 39.0 76-77 30.09927160003754 35.0 30.0 36.0 2.0 38.5 78-79 29.826307852741497 35.0 30.0 35.5 2.0 37.0 80-81 29.592428277726967 35.0 30.0 35.0 2.0 37.0 82-83 29.397884107695475 35.0 30.0 35.0 2.0 36.5 84-85 29.19686034900944 35.0 30.0 35.0 2.0 36.0 86-87 29.04464451738548 34.5 30.0 35.0 2.0 36.0 88-89 28.919947701628814 34.0 30.0 35.0 2.0 35.5 90-91 28.813248998420907 34.0 29.0 35.0 2.0 35.0 92-93 28.706290610367226 34.0 29.0 35.0 2.0 35.0 94-95 28.641893140414595 34.0 29.0 35.0 2.0 35.0 96-97 28.542797578140075 34.0 29.0 35.0 2.0 35.0 98-99 28.483891378871157 34.0 29.0 35.0 2.0 35.0 100-101 28.414442441189106 34.0 29.0 35.0 2.0 35.0 102-103 27.467167499931506 32.5 26.5 34.5 2.0 35.0 104-105 28.26142278150158 34.0 29.0 35.0 2.0 35.0 106-107 28.56945010349505 34.5 29.5 35.0 2.0 35.0 108-109 28.60227065398029 35.0 30.0 35.0 2.0 35.0 110-111 28.54238400597829 35.0 30.0 35.0 2.0 35.0 112-113 28.471784993771642 35.0 30.0 35.0 2.0 35.0 114-115 28.349063356690397 35.0 29.0 35.0 2.0 35.0 116-117 28.288085507720304 35.0 29.0 35.0 2.0 35.0 118-119 28.131751733126748 34.5 29.0 35.0 2.0 35.0 120-121 27.958571377775293 34.0 29.0 35.0 2.0 35.0 122-123 27.82863539839619 34.0 29.0 35.0 2.0 35.0 124-125 26.994234763261268 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68895.0 3 12281.0 4 7847.0 5 6808.0 6 6969.0 7 8151.0 8 9137.0 9 9627.0 10 10038.0 11 10571.0 12 11065.0 13 11706.0 14 13369.0 15 11597.0 16 8065.0 17 7038.0 18 6392.0 19 5845.0 20 5532.0 21 5805.0 22 7085.0 23 9506.0 24 13578.0 25 18774.0 26 22051.0 27 23381.0 28 27187.0 29 32747.0 30 36442.0 31 43720.0 32 54921.0 33 71476.0 34 99820.0 35 155469.0 36 293701.0 37 440070.0 38 128680.0 39 195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.95122389264218 17.00737869240754 12.47631109696094 24.565086317989337 2 16.724254142100158 19.1784951302336 38.77405729905217 25.323193428614076 3 19.18546073652541 24.25566154348485 28.771019427665184 27.787858292324557 4 13.344579426064424 15.053826139993445 35.51866334376309 36.08293109017905 5 14.284739654855724 37.339899179852296 33.22854802077047 15.146813144521513 6 33.49370722026566 34.481143821552266 17.27022650041435 14.754922457767725 7 29.807069361145043 31.46871619631866 20.42318199974925 18.30103244278704 8 27.950778137170694 33.606580797222506 19.32861360441161 19.11402746119519 9 28.039437601930718 14.6755155791822 18.710178873279677 38.57486794560741 10-11 26.51388876335733 24.951987822239925 26.61167703978502 21.92244637461773 12-13 26.66436725442598 22.585106922669194 26.94353300511799 23.80699281778684 14-15 24.180723260400857 23.670093895276374 24.355568772713955 27.793614071608815 16-17 24.215454653332902 26.81966733230528 24.82181848504383 24.143059529317984 18-19 24.319103818150857 25.881374179716932 26.48213748329107 23.31738451884114 20-21 25.512274703244923 24.725263508472924 26.17254052439805 23.5899212638841 22-23 25.16980120770207 24.336156087341582 26.110061452421107 24.383981252535243 24-25 24.653588618055476 24.760506521379643 26.153909867565872 24.431994992999005 26-27 24.31452354826773 24.81933444392508 26.231183678325355 24.634958329481837 28-29 24.330272136813146 25.113796146123292 26.13713040673848 24.41880131032508 30-31 24.793631104062293 24.857927746902753 26.05165834309447 24.296782805940484 32-33 24.38335239563898 24.86365250884633 26.07408461564035 24.678910479874347 34-35 24.65613670676145 24.983919904504933 26.222743290532772 24.137200098200836 36-37 24.713006847243854 24.909900465493607 26.009816103343486 24.36727658391905 38-39 24.828059459653357 24.999803146563654 25.894246834378016 24.277890559404973 40-41 24.81092289339804 24.908490383653685 25.977737933021345 24.30284878992693 42-43 24.60356020087372 24.990726213320425 25.91384710500376 24.491866480802095 44-45 24.519763136904942 25.00069262478547 25.894659789996165 24.58488444831342 46-47 24.612707295434383 25.04312001684553 25.751822356613186 24.5923503311069 48-49 24.76240492406172 25.250512923095947 25.44847965978531 24.53860249305702 50-51 24.755189778405963 25.426932521581 25.351348340720193 24.466529359292842 52-53 24.865849006641056 25.297552735922018 25.425958567174067 24.410639690262855 54-55 24.5773041240841 25.180584682639847 25.691487996790123 24.550623196485933 56-57 24.58071986398053 25.228800974133613 25.378268434344392 24.812210727541466 58-59 24.66156293236799 25.18434822416581 25.437765981487175 24.716322861979027 60-61 24.804041133212962 25.21174636559532 25.628110669346217 24.356101831845496 62-63 24.590791067776028 25.366332274416443 25.55585847349768 24.487018184309857 64-65 24.569398861216275 25.01784452173183 25.6807743356123 24.7319822814396 66-67 24.494907086694983 25.266220468216815 25.606321416825857 24.632551028262345 68-69 24.589973714469636 25.25171835532346 25.509238419323015 24.64906951088389 70-71 24.443327939701746 25.082836325102726 25.695838252871606 24.777997482323926 72-73 24.687309857483676 25.40567867048125 25.372879769725476 24.534131702309598 74-75 24.500735779283126 25.531643174309565 25.37273700854624 24.59488403786107 76-77 24.479993935789338 25.140322336120533 25.466973375782807 24.912710352307315 78-79 24.498732782947425 25.13809939891375 25.601603751883772 24.76156406625506 80-81 24.51294124918045 25.41774859025611 25.268662507765505 24.80064765279793 82-83 24.692311347306237 25.37527361195768 25.289717495744785 24.642697544991293 84-85 24.501010499104645 25.475771196067726 25.22802182666406 24.795196478163568 86-87 24.18727869357096 25.626143972230132 25.336126333304282 24.850451000894626 88-89 24.33672652834434 25.375441251879938 25.375441251879938 24.912390967895785 90-91 24.411890217952738 25.21033902593443 25.41659719493554 24.96117356117729 92-93 24.44641440714504 25.485561634195808 25.283282547358056 24.784741411301102 94-95 24.443444284963057 25.265728900553963 25.332948168289526 24.957878646193453 96-97 24.371591612302012 25.352326980191453 25.329386995214666 24.946694412291865 98-99 24.26835137089659 25.30076127076865 25.250713336098553 25.18017402223621 100-101 24.239610972769203 25.40945594076563 25.37841281297084 24.97252027349433 102-103 24.306066439008582 25.16686700422866 25.55915748630962 24.967909070453143 104-105 24.262836833252255 25.305802665140142 25.387268431010156 25.044092070597447 106-107 24.191994151787693 25.312405998381713 25.44884679689121 25.04675305293938 108-109 24.35356795046054 25.195772565985518 25.410047515929246 25.04061196762469 110-111 24.235995089289357 25.462710894152735 25.39141698252454 24.90987703403337 112-113 24.186468812803955 25.45768279548737 25.38568824025778 24.970160151450894 114-115 23.96586223932867 25.656748197580452 25.45020775007615 24.92718181301473 116-117 24.04247015229246 25.59139427655033 25.312715599972368 25.053419971184844 118-119 24.093256074709565 25.265866229306667 25.443344097889486 25.197533598094278 120-121 23.794211466273723 25.383288397720243 25.659512261894385 25.162987874111646 122-123 23.90492951895407 25.472120827482144 25.462471280937947 25.16047837262584 124-125 23.78741840852355 25.512530731149987 25.491020747807102 25.209030112519365 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 219.5 2 268.0 3 273.0 4 246.0 5 225.0 6 223.0 7 269.5 8 380.0 9 469.5 10 516.5 11 577.0 12 639.5 13 719.5 14 822.0 15 924.0 16 1062.0 17 1154.5 18 1226.5 19 1342.5 20 1469.0 21 1663.0 22 1959.5 23 2361.5 24 2796.0 25 3399.5 26 4431.5 27 5552.5 28 6366.0 29 7676.0 30 9650.5 31 11401.0 32 13443.5 33 15672.0 34 17993.5 35 20484.5 36 23704.5 37 27178.5 38 30009.0 39 33118.0 40 35839.0 41 38310.5 42 41033.5 43 45957.5 44 51552.5 45 55538.0 46 59461.5 47 63176.5 48 67849.5 49 72822.0 50 73190.0 51 71953.0 52 71871.5 53 72081.0 54 72463.5 55 71220.5 56 67507.5 57 61915.0 58 57996.0 59 54037.0 60 48096.0 61 42488.5 62 37014.5 63 29109.0 64 21670.5 65 17179.0 66 13199.5 67 9879.5 68 7809.5 69 6379.5 70 5403.0 71 4463.5 72 3635.5 73 3298.0 74 2687.5 75 1954.5 76 1379.5 77 811.5 78 464.0 79 288.0 80 164.5 81 91.5 82 65.5 83 38.0 84 26.5 85 24.5 86 18.0 87 10.5 88 8.0 89 8.0 90 8.5 91 9.0 92 12.0 93 12.0 94 6.5 95 4.5 96 5.0 97 4.0 98 6.5 99 10.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03514926195293497 2 0.027338314852282747 3 0.02687198965224381 4 0.03351712375279868 5 0.03532413390294956 6 0.04651593870388408 7 0.039404479403290275 8 0.035673877802978766 9 0.030777463202569917 10-11 0.03564473247797633 12-13 0.03555729650296904 14-15 0.03488695402791306 16-17 0.03768490522814669 18-19 0.051820387854327 20-21 0.06295390200525665 22-23 0.05610475062968475 24-25 0.06653877698055599 26-27 0.048935000679086064 28-29 0.049663633804146914 30-31 0.048526966129052 32-33 0.057591162204808866 34-35 0.04010396720334868 36-37 0.05301534617942678 38-39 0.062225268880195805 40-41 0.048410384829042266 42-43 0.060097660155018155 44-45 0.06111774653010334 46-47 0.06645134100554868 48-49 0.05007166835418098 50-51 0.052257567729363505 52-53 0.061496635755134976 54-55 0.04855611145405443 56-57 0.04584559622882811 58-59 0.04567072427881351 60-61 0.03360455972780598 62-63 0.043047645028594476 64-65 0.04249388385354824 66-67 0.035499005852964165 68-69 0.030835753852574786 70-71 0.05039226692920776 72-73 0.03173925892765023 74-75 0.040366275128370585 76-77 0.032701054652730537 78-79 0.032788490627737844 80-81 0.024744380927066156 82-83 0.017050015126423675 84-85 0.017458049676457747 86-87 0.017516340326462612 88-89 0.022879080126910402 90-91 0.012532489751046464 92-93 0.019614803726637837 94-95 0.015097278351260623 96-97 0.01139582207595155 98-99 0.010812915575902879 100-101 0.010812915575902879 102-103 0.006003936950501329 104-105 0.0056250477254696915 106-107 0.008452144250705755 108-109 0.012911378976078101 110-111 0.006237099550520798 112-113 0.00778180177564978 114-115 0.008073255025674117 116-117 0.007257185925605974 118-119 0.23666003901976113 120-121 0.3531247577294859 122-123 0.02532728742711483 124-125 0.0034100030252847357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1715541.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.56655611109911 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87549252360805 45.9815702226196 2 10.319191543011724 11.880806379639278 3 3.599401511125064 6.216154471696677 4 1.7582338858889421 4.048618785938466 5 1.0699779807636156 3.079747373363461 6 0.6972560698902434 2.4083178402684684 7 0.4833472980379138 1.9477247547553402 8 0.35440740527587594 1.6321611025602207 9 0.2658249357359782 1.3772363470897055 >10 1.4713120653656226 15.092362662399328 >50 0.0784061517925583 3.0720781263133174 >100 0.025924042444027004 2.597570443441467 >500 8.163913735475752E-4 0.2793374666441283 >1k 4.081956867737876E-4 0.38631402327061776 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2301 0.1341267856611996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1869 0.10894522485909693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 5.829065000486727E-5 10-11 0.0 0.0 0.0 0.0 8.743597500730091E-5 12-13 0.0 0.0 0.0 5.829065000486727E-5 2.331626000194691E-4 14-15 0.0 0.0 0.0 5.829065000486727E-5 2.331626000194691E-4 16-17 0.0 0.0 0.0 5.829065000486727E-5 2.331626000194691E-4 18-19 0.0 0.0 0.0 5.829065000486727E-5 2.331626000194691E-4 20-21 0.0 0.0 0.0 5.829065000486727E-5 3.4974390002920363E-4 22-23 0.0 0.0 0.0 5.829065000486727E-5 3.4974390002920363E-4 24-25 0.0 0.0 0.0 5.829065000486727E-5 3.788892250316373E-4 26-27 0.0 2.9145325002433636E-5 0.0 1.1658130000973454E-4 4.080345500340709E-4 28-29 0.0 5.829065000486727E-5 0.0 8.452144250705755E-4 4.080345500340709E-4 30-31 0.0 5.829065000486727E-5 0.0 0.0037306016003115054 4.371798750365045E-4 32-33 0.0 5.829065000486727E-5 0.0 0.00915163205076416 4.954705250413717E-4 34-35 0.0 5.829065000486727E-5 0.0 0.016000783426336065 5.246158500438054E-4 36-37 0.0 5.829065000486727E-5 0.0 0.025443868727124563 5.246158500438054E-4 38-39 0.0 5.829065000486727E-5 0.0 0.04118234422843872 5.246158500438054E-4 40-41 0.0 5.829065000486727E-5 0.0 0.061554926405139834 5.246158500438054E-4 42-43 0.0 5.829065000486727E-5 0.0 0.08271443235690665 5.246158500438054E-4 44-45 0.0 5.829065000486727E-5 0.0 0.10439855415871727 5.246158500438054E-4 46-47 0.0 5.829065000486727E-5 0.0 0.13062934666090753 5.246158500438054E-4 48-49 0.0 5.829065000486727E-5 0.0 0.15761791761316107 5.246158500438054E-4 50-51 0.0 5.829065000486727E-5 0.0 0.18956119381582837 6.120518250511064E-4 52-53 0.0 5.829065000486727E-5 0.0 0.2213878887184859 6.994878000584073E-4 54-55 0.0 5.829065000486727E-5 0.0 0.25607082547138194 7.577784500632745E-4 56-57 0.0 5.829065000486727E-5 0.0 0.29428034654957236 7.869237750657081E-4 58-59 0.0 5.829065000486727E-5 0.0 0.33677423040312066 8.452144250705755E-4 60-61 0.0 5.829065000486727E-5 0.0 0.3836107676820315 8.74359750073009E-4 62-63 0.0 5.829065000486727E-5 0.0 0.43572260878638286 8.74359750073009E-4 64-65 0.0 5.829065000486727E-5 0.0 0.48713496209067575 8.74359750073009E-4 66-67 0.0 5.829065000486727E-5 0.0 0.54239449829529 9.326504000778764E-4 68-69 0.0 5.829065000486727E-5 0.0 0.6072719917507072 0.0010492317000876108 70-71 0.0 5.829065000486727E-5 0.0 0.6823503489569762 0.0011075223500924781 72-73 0.0 5.829065000486727E-5 0.0 0.7607221278885203 0.0014572662501216816 74-75 0.0 5.829065000486727E-5 0.0 0.8396476679951106 0.0014864115751241154 76-77 0.0 5.829065000486727E-5 0.0 0.9288615078275599 0.001515556900126549 78-79 0.0 5.829065000486727E-5 0.0 1.032035958336175 0.001515556900126549 80-81 0.0 1.1658130000973454E-4 0.0 1.143808279720508 0.0015447022251289825 82-83 0.0 1.1658130000973454E-4 0.0 1.2650528317306318 0.0015738475501314163 84-85 0.0 1.1658130000973454E-4 0.0 1.3878712312908874 0.0015738475501314163 86-87 0.0 1.1658130000973454E-4 0.0 1.5185005779517948 0.00160299287513385 88-89 0.0 1.1658130000973454E-4 0.0 1.669007036264362 0.0016612835251387172 90-91 0.0 1.1658130000973454E-4 0.0 1.8335615412281023 0.0016904288501411507 92-93 0.0 1.1658130000973454E-4 0.0 2.0116686223179743 0.0016904288501411507 94-95 5.829065000486727E-5 1.1658130000973454E-4 0.0 2.1910580977079537 0.001748719500146018 96-97 5.829065000486727E-5 1.1658130000973454E-4 0.0 2.3855157061241905 0.0018070101501508854 98-99 5.829065000486727E-5 1.1658130000973454E-4 0.0 2.601045384517187 0.0018070101501508854 100-101 5.829065000486727E-5 1.1658130000973454E-4 0.0 2.849800733412958 0.0018070101501508854 102-103 5.829065000486727E-5 1.1658130000973454E-4 0.0 3.113624215334988 0.0020401727501703545 104-105 5.829065000486727E-5 1.1658130000973454E-4 0.0 3.3987237845087934 0.0020984634001752216 106-107 5.829065000486727E-5 1.1658130000973454E-4 0.0 3.688661477633003 0.0020984634001752216 108-109 5.829065000486727E-5 1.1658130000973454E-4 0.0 3.986876443057904 0.0021567540501800887 110-111 5.829065000486727E-5 1.1658130000973454E-4 0.0 4.321115030185814 0.0022441900251873896 112-113 5.829065000486727E-5 1.1658130000973454E-4 0.0 4.680680904740837 0.0022733353501898233 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2095 0.0 71.85785 1 GTATCAA 4075 0.0 60.45197 1 TCAACGC 5480 0.0 44.62193 4 CAACGCA 5610 0.0 43.692684 5 AACGCAG 5860 0.0 41.82988 6 ATCAACG 5950 0.0 41.19596 3 TATCAAC 6145 0.0 40.176796 2 GTACATG 9640 0.0 36.97324 1 ACGCAGA 6995 0.0 34.87149 7 TACATGG 10040 0.0 34.6634 2 ACATGGG 10075 0.0 34.013535 3 CGCAGAG 7440 0.0 32.621075 8 CATGGGG 8775 0.0 30.375116 4 GCAGAGT 7960 0.0 29.819214 9 GTATAGG 525 0.0 29.468077 1 AGAGTAC 7470 0.0 26.400105 10-11 CAGAGTA 7840 0.0 24.850662 10-11 CATGGGT 1745 0.0 24.548452 4 ATGGGGG 4880 0.0 24.504768 5 ATGGGGA 3205 0.0 23.94617 5 >>END_MODULE