FastQCFastQC Report
Mon 15 Aug 2016
SRR2921519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715541
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.21625831151805755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.1959148746663589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.11570694025966152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.080.522831
GTATCAA56800.059.7287371
TCAACGC78950.042.6874664
CAACGCA80850.041.5827455
ATCAACG83600.040.3131073
AACGCAG84500.039.778456
TATCAAC84400.039.719342
ACGCAGA98050.034.2176067
CGCAGAG103300.032.420048
GTACATG94150.032.0442121
TACATGG98300.030.3473172
GCAGAGT108700.030.2613569
ACATGGG98000.030.0756663
GTATAGG5600.029.8118821
TATACCG2701.8189894E-1228.649985
CATGGGG83550.028.0792734
ATGGGGG45400.027.2616545
AGAGTAC103600.027.0724410-11
CAGAGTA107950.025.87131110-11
GAGTACT61400.024.60912112-13
Produced by FastQC (version 0.11.5)