FastQCFastQC Report
Mon 15 Aug 2016
SRR2921518_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921518_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561917
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18100.067.0714651
GTATCAA36850.062.497071
TCAACGC47450.048.523224
CAACGCA49150.046.9659465
ATCAACG50700.045.1751753
AACGCAG51250.044.923966
TATCAAC54800.041.7952772
ACGCAGA59850.037.971717
GTACATG86750.036.7003751
CGCAGAG64000.035.3179138
TACATGG89700.034.6861652
ACATGGG94050.032.9553533
GCAGAGT69350.032.422819
CATGGGG76000.030.5299364
ATGGGGG40600.027.6955915
AGAGTAC64100.027.33043110-11
GTATAGA4650.026.8752521
CAGAGTA67850.025.60072110-11
ATGGGGA28550.024.3811765
GAGTACT24950.021.57765412-13
Produced by FastQC (version 0.11.5)