##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921518_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561917
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.94402775563618	31.0	30.0	34.0	26.0	34.0
2	30.054626462225585	31.0	30.0	34.0	25.0	34.0
3	30.219568005214107	31.0	31.0	34.0	26.0	34.0
4	33.588361609483734	37.0	35.0	37.0	28.0	37.0
5	33.66390147491832	37.0	35.0	37.0	28.0	37.0
6	33.64997691938816	37.0	35.0	37.0	28.0	37.0
7	33.47923097065977	37.0	35.0	37.0	27.0	37.0
8	33.48145259959396	37.0	35.0	37.0	27.0	37.0
9	34.94456235510594	39.0	35.0	39.0	27.0	39.0
10-11	34.836687864976184	39.0	35.0	39.0	27.0	39.0
12-13	34.70376242783708	39.0	35.0	39.0	26.5	39.0
14-15	35.88393429356361	40.0	36.0	41.0	27.0	41.0
16-17	35.82172420173415	40.0	36.0	41.0	26.0	41.0
18-19	35.84162922869781	40.0	36.0	41.0	26.0	41.0
20-21	35.87191540907743	40.0	36.0	41.0	26.0	41.0
22-23	35.846384282903635	40.0	36.0	41.0	26.0	41.0
24-25	35.80695293027735	40.0	36.0	41.0	26.0	41.0
26-27	35.734289017918364	40.0	36.0	41.0	26.0	41.0
28-29	35.69620408766919	40.0	35.5	41.0	26.0	41.0
30-31	35.598276668990735	40.0	35.0	41.0	26.0	41.0
32-33	35.495542016637245	40.0	35.0	41.0	25.0	41.0
34-35	35.34243272849966	40.0	35.0	41.0	25.0	41.0
36-37	35.21363171026373	40.0	35.0	41.0	25.0	41.0
38-39	35.094523268521954	40.0	35.0	41.0	24.0	41.0
40-41	34.950620935683524	39.0	35.0	41.0	24.0	41.0
42-43	34.75476065629608	39.0	34.5	41.0	23.0	41.0
44-45	34.57005910045156	39.0	34.0	41.0	22.5	41.0
46-47	34.323645878750284	39.0	34.0	41.0	21.0	41.0
48-49	34.17847875399269	39.0	33.5	41.0	20.0	41.0
50-51	33.989476073312474	38.5	33.0	41.0	18.0	41.0
52-53	33.799554649830945	38.0	33.0	40.0	18.0	41.0
54-55	33.60149258891478	38.0	33.0	40.0	16.5	41.0
56-57	33.36256311955117	37.5	33.0	40.0	14.5	41.0
58-59	33.122008723895064	37.0	33.0	40.0	13.5	41.0
60-61	32.811231966871475	36.5	32.0	40.0	12.0	41.0
62-63	32.48943669862099	36.0	32.0	39.5	8.0	41.0
64-65	32.184355826846115	35.5	32.0	39.0	8.0	41.0
66-67	32.14162404276284	35.0	32.0	39.0	7.5	41.0
68-69	31.889895557830535	35.0	32.0	39.0	7.0	40.5
70-71	31.585948229003208	35.0	32.0	37.5	4.0	40.0
72-73	31.252688523141757	35.0	31.0	37.0	2.0	39.5
74-75	30.943181679948424	35.0	31.0	36.5	2.0	39.0
76-77	30.659302959120108	35.0	31.0	36.0	2.0	39.0
78-79	30.385409724076247	35.0	31.0	36.0	2.0	37.0
80-81	30.142801442074067	35.0	31.0	35.0	2.0	37.0
82-83	29.94216177940313	35.0	31.0	35.0	2.0	36.5
84-85	29.735222486214056	35.0	30.5	35.0	2.0	36.0
86-87	29.582732629198606	35.0	30.0	35.0	2.0	36.0
88-89	29.447274727146194	35.0	30.0	35.0	2.0	36.0
90-91	29.336159988014728	35.0	30.0	35.0	2.0	35.0
92-93	29.234287417321152	34.0	30.0	35.0	2.0	35.0
94-95	29.162948159217166	34.0	30.0	35.0	2.0	35.0
96-97	29.063371485168545	34.0	30.0	35.0	2.0	35.0
98-99	28.997953156281675	34.0	30.0	35.0	2.0	35.0
100-101	28.931865777758997	34.0	30.0	35.0	2.0	35.0
102-103	27.957669645698203	33.5	28.0	34.5	2.0	35.0
104-105	28.771453924888455	34.0	29.5	35.0	2.0	35.0
106-107	29.083310764912603	35.0	30.0	35.0	2.0	35.0
108-109	29.119865844343842	35.0	31.0	35.0	2.0	35.0
110-111	29.058149696814873	35.0	30.0	35.0	2.0	35.0
112-113	28.990982555411076	35.0	30.0	35.0	2.0	35.0
114-115	28.868640267056442	35.0	30.0	35.0	2.0	35.0
116-117	28.80105504966013	35.0	30.0	35.0	2.0	35.0
118-119	28.63997478739267	35.0	30.0	35.0	2.0	35.0
120-121	28.464869131970524	35.0	30.0	35.0	2.0	35.0
122-123	28.338442119523634	35.0	30.0	35.0	2.0	35.0
124-125	27.481719579209397	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53830.0
3	9736.0
4	6322.0
5	5466.0
6	5890.0
7	6619.0
8	7529.0
9	7708.0
10	8530.0
11	8817.0
12	9047.0
13	9537.0
14	10829.0
15	9373.0
16	6647.0
17	5852.0
18	5435.0
19	5106.0
20	4901.0
21	5246.0
22	6200.0
23	8238.0
24	11844.0
25	16016.0
26	19497.0
27	20570.0
28	23718.0
29	28602.0
30	32393.0
31	38350.0
32	49106.0
33	62978.0
34	89605.0
35	141292.0
36	272889.0
37	423961.0
38	124097.0
39	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.514801858382256	17.42547192892341	12.375188777974046	24.684537434720287
2	16.576111668274113	19.513897694626003	38.75299234957559	25.15699828752429
3	19.017851710722233	24.515217982996845	29.046510510895146	27.420419795385776
4	13.310447522215995	15.437290849411575	36.0044191818109	35.24784244656152
5	14.153484731841722	37.49719794486068	33.5529931072646	14.796324216033007
6	32.97973741659748	35.078316370978484	17.56667076632947	14.375275446094559
7	29.50867133476297	31.62270150060593	20.756759388074578	18.111867776556522
8	27.71804222820859	33.65863375105519	19.65150135205588	18.971822668680343
9	28.040438197893554	14.639344944744922	19.00114961300632	38.31906724435521
10-11	26.2674419684561	25.103848786047646	27.01717720248936	21.611532043006896
12-13	26.496679028239466	22.922359074867575	27.078473569932555	23.502488326960396
14-15	24.156701744048267	23.877288943259188	24.499202592694598	27.466806719997948
16-17	24.031481921529622	26.893113778016332	25.03873431812548	24.03666998232857
18-19	24.118222656406395	26.151293610819724	26.554871359592582	23.1756123731813
20-21	25.37248457590959	25.063041765169423	26.17719605092032	23.387277608000666
22-23	25.164687259167106	24.73457532372105	25.933879098443356	24.166858318668485
24-25	24.576514434612573	25.153495297594752	26.051690258246268	24.218300009546404
26-27	24.13195044987912	25.235541155323922	26.224436065661983	24.408072329134974
28-29	24.216787936111174	25.316876946399407	26.265273720248892	24.20106139724052
30-31	24.79541210647859	25.16820247715502	25.97640072642715	24.05998468993924
32-33	24.318050414892237	25.2881663220576	25.970956742619467	24.4228265204307
34-35	24.475231315198766	25.37653672363971	26.112684473290543	24.035547487870986
36-37	24.62025170495966	25.156178532946754	25.917853166554988	24.3057165955386
38-39	24.493353574097632	25.44925879786133	25.91220381625324	24.1451838117878
40-41	24.630195708655968	25.13721129257906	26.108003607721752	24.124589391043216
42-43	24.437548288396563	25.26167025639777	25.832418282899855	24.468363172305814
44-45	24.40086769118804	25.317760505956727	25.885180828791153	24.39619097406408
46-47	24.503233188731986	25.308663331289566	25.767101495794257	24.421001984184187
48-49	24.478488386617787	25.556903619080806	25.541433629259615	24.423174365041795
50-51	24.532951625296633	25.609340102693466	25.45332366891974	24.404384603090158
52-53	24.622644742940995	25.492337256185788	25.554705260726767	24.330312740146447
54-55	24.430794005849638	25.313876814240015	25.70868900700906	24.546640172901284
56-57	24.38373924163904	25.491466493548586	25.545208255733918	24.57958600907845
58-59	24.558308017762805	25.394786780708174	25.52373204622941	24.523173155299617
60-61	24.669331675788605	25.520104294661238	25.644388125498168	24.166175904051986
62-63	24.444699948692755	25.523689407327648	25.661917917374193	24.3696927266054
64-65	24.417288573546	25.1848426171524	25.756873109218397	24.640995700083206
66-67	24.37454185268769	25.430638458402544	25.651157629575827	24.54366205933394
68-69	24.366510686004297	25.455429270246828	25.622809874270814	24.555250169478064
70-71	24.353183619686252	25.22300423619968	25.790147648822696	24.63366449529137
72-73	24.522015086086117	25.53807627584065	25.443319899639583	24.49658873843365
74-75	24.238546207326802	25.671189032054958	25.522621274556993	24.567643486061243
76-77	24.353679890455737	25.25800927129753	25.58730172962601	24.80100910862072
78-79	24.286977375864108	25.389731475085718	25.630386517937687	24.692904631112487
80-81	24.33704076682554	25.64052891365848	25.436270515096837	24.586159804419143
82-83	24.48531598887945	25.531669648091544	25.52488179693631	24.4581325660927
84-85	24.199825950978113	25.57361575636723	25.37686608022776	24.849692212426895
86-87	24.06045105521357	25.671174998895385	25.591290365753157	24.677083580137896
88-89	24.22702364157182	25.42488804242509	25.56699235172706	24.78109596427604
90-91	24.204218491220736	25.39428495321983	25.631555474590666	24.769941080968767
92-93	24.30191656362045	25.568024272587614	25.5508303358677	24.579228827924236
94-95	24.25568223658723	25.288490169146392	25.637060445101863	24.818767149164515
96-97	24.251384853978024	25.297829248192855	25.60265400197347	24.84813189585565
98-99	24.063071978393324	25.35810229554929	25.485268189733308	25.09355753632408
100-101	24.06293261208375	25.550317787792288	25.58162913786855	24.805120462255417
102-103	24.134623665139497	25.28078610826276	25.681856525765923	24.902733700831817
104-105	24.018215869255517	25.475165271397245	25.573159604949335	24.9334592543979
106-107	24.05237387555538	25.35182814894117	25.60244107490791	24.993356900595543
108-109	24.012314729971756	25.415298772432987	25.50343950561789	25.06894699197737
110-111	23.956674946528782	25.56835080065167	25.628440894173888	24.846533358645658
112-113	23.884030689096022	25.61377391682954	25.63871320934119	24.863482184733247
114-115	23.729784310486355	25.783688120365074	25.60629460449666	24.880232964651917
116-117	23.643949262568224	25.812881309484442	25.567748352787845	24.97542107515949
118-119	23.793831252705683	25.37412627919209	25.624661639272084	25.207380828830146
120-121	23.585611878765448	25.507114510723174	25.833795982152036	25.073477628359342
122-123	23.629436577369145	25.66211037003379	25.639471381212076	25.068981671384993
124-125	23.559604021760045	25.61901612411151	25.6236900579984	25.197689796130046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	160.0
1	164.5
2	180.0
3	183.5
4	172.0
5	139.0
6	131.0
7	177.0
8	239.5
9	293.0
10	301.0
11	337.5
12	390.0
13	424.5
14	499.0
15	554.5
16	623.5
17	708.5
18	787.0
19	912.5
20	1031.5
21	1219.0
22	1446.5
23	1695.5
24	2045.0
25	2660.5
26	3878.5
27	5053.5
28	5721.0
29	7111.5
30	9370.0
31	11469.5
32	14341.0
33	17554.5
34	20626.5
35	23520.0
36	26601.5
37	29915.5
38	31733.5
39	33517.0
40	35548.0
41	37067.5
42	38306.0
43	41423.0
44	45736.5
45	48832.0
46	52138.0
47	55613.5
48	59456.0
49	63443.5
50	64548.0
51	64302.0
52	63885.0
53	63638.5
54	63913.0
55	63634.0
56	61375.0
57	56417.5
58	51993.0
59	47509.5
60	42227.5
61	37355.5
62	32128.0
63	25262.0
64	19007.5
65	14935.0
66	11702.0
67	9204.0
68	7338.5
69	5804.0
70	4721.5
71	3801.5
72	2987.5
73	2530.5
74	2018.5
75	1536.5
76	1082.0
77	614.0
78	366.5
79	233.0
80	134.0
81	68.0
82	45.0
83	37.5
84	26.0
85	16.5
86	16.0
87	14.5
88	9.0
89	5.0
90	8.0
91	7.5
92	8.5
93	9.0
94	6.0
95	5.5
96	6.0
97	6.0
98	6.5
99	9.0
100	17.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03578935372366137
2	0.027850391538090692
3	0.028298558758243872
4	0.03470094761757507
5	0.03604544927803462
6	0.052051421426362605
7	0.04360026813204543
8	0.037838118158647356
9	0.03342046984570883
10-11	0.040495109535269795
12-13	0.04014297814800658
14-15	0.03886250037614034
16-17	0.04097528869971964
18-19	0.05630901001781785
20-21	0.0670330113571976
22-23	0.05941416861459348
24-25	0.07170675522450937
26-27	0.05320385142104222
28-29	0.05515658002313823
30-31	0.05454835308150177
32-33	0.06207115999121592
34-35	0.04363228007634209
36-37	0.056533093627894444
38-39	0.06661685608134106
40-41	0.05256361253510909
42-43	0.06293548248722564
44-45	0.06357572137315876
46-47	0.06876165634921702
48-49	0.05313982753244891
50-51	0.05528462780032485
52-53	0.06533637830947484
54-55	0.050898991431682986
56-57	0.04785785672350067
58-59	0.05054686004441977
60-61	0.03658965233107778
62-63	0.04699353422749096
64-65	0.04635329534155784
66-67	0.03851036898887713
68-69	0.032364075683919184
70-71	0.05429225752712852
72-73	0.03486100733905835
74-75	0.04331216063337553
76-77	0.03450887595179513
78-79	0.03543722233639816
80-81	0.026537901821927796
82-83	0.01943125018807017
84-85	0.018438879914873838
86-87	0.01856692769206046
88-89	0.024841268774205033
90-91	0.012644717997179106
92-93	0.02048764434985982
94-95	0.01613401992551461
96-97	0.012804777718662386
98-99	0.011364240225312868
100-101	0.011716371612576083
102-103	0.0061783052492546025
104-105	0.005890197750584698
106-107	0.009347487734623542
108-109	0.013445016604595508
110-111	0.00611428136066129
112-113	0.007970974129867336
114-115	0.008643224960097113
116-117	0.0071386635781542805
118-119	0.24671605469432753
120-121	0.3624392333267389
122-123	0.028906785699880342
124-125	0.004065516925675308
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1561917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.722388629546415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01012145865553	46.18375325133726
2	10.418698431615114	12.027843197674667
3	3.495525328833213	6.053102144860012
4	1.7199982126134912	3.971296210824045
5	1.0085178396907641	2.9107029341230435
6	0.6564695434359018	2.2735794065800814
7	0.4859308218255921	1.9634361421144189
8	0.34410033695704084	1.5889834701913748
9	0.2616193534931497	1.3591164595808038
>10	1.4859712665669216	15.454024133502076
>50	0.08313486510960152	3.2319510586869797
>100	0.029464438915607315	2.7348724176799513
>500	2.2405114401266398E-4	0.08975340442069862
>1k	2.2405114401266398E-4	0.15758576842452632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.402388859331194E-5
2	0.0	0.0	0.0	0.0	6.402388859331194E-5
3	0.0	0.0	0.0	0.0	6.402388859331194E-5
4	0.0	0.0	0.0	0.0	6.402388859331194E-5
5	0.0	0.0	0.0	0.0	6.402388859331194E-5
6	0.0	0.0	0.0	0.0	2.5609555437324774E-4
7	0.0	0.0	0.0	0.0	2.5609555437324774E-4
8	0.0	0.0	0.0	0.0	2.5609555437324774E-4
9	0.0	0.0	0.0	0.0	2.5609555437324774E-4
10-11	0.0	0.0	0.0	0.0	2.5609555437324774E-4
12-13	0.0	0.0	0.0	6.402388859331194E-5	2.881074986699037E-4
14-15	0.0	0.0	0.0	6.402388859331194E-5	3.201194429665597E-4
16-17	0.0	0.0	0.0	6.402388859331194E-5	3.841433315598716E-4
18-19	0.0	0.0	0.0	6.402388859331194E-5	3.841433315598716E-4
20-21	0.0	0.0	0.0	6.402388859331194E-5	3.841433315598716E-4
22-23	0.0	0.0	0.0	1.2804777718662387E-4	4.161552758565276E-4
24-25	0.0	0.0	0.0	3.841433315598716E-4	4.4816722015318357E-4
26-27	0.0	0.0	0.0	4.8017916444983955E-4	4.4816722015318357E-4
28-29	0.0	0.0	0.0	0.0013445016604595507	4.4816722015318357E-4
30-31	0.0	0.0	0.0	0.00531398275324489	4.4816722015318357E-4
32-33	0.0	0.0	0.0	0.012260574665619237	4.4816722015318357E-4
34-35	0.0	0.0	0.0	0.02141599073446284	5.121911087464955E-4
36-37	0.0	0.0	0.0	0.037646046492867416	5.121911087464955E-4
38-39	0.0	0.0	0.0	0.062071159991215916	5.762149973398074E-4
40-41	0.0	0.0	0.0	0.08758467959565072	5.762149973398074E-4
42-43	0.0	0.0	0.0	0.11476282030351165	5.762149973398074E-4
44-45	0.0	0.0	0.0	0.14523819127392812	5.762149973398074E-4
46-47	3.201194429665597E-5	0.0	0.0	0.1810915688861828	6.402388859331194E-4
48-49	6.402388859331194E-5	0.0	0.0	0.21806536454882045	7.042627745264313E-4
50-51	6.402388859331194E-5	0.0	0.0	0.25964888019017657	7.042627745264313E-4
52-53	6.402388859331194E-5	0.0	0.0	0.2989595477864701	7.042627745264313E-4
54-55	6.402388859331194E-5	0.0	0.0	0.34294395925007537	7.042627745264313E-4
56-57	6.402388859331194E-5	0.0	0.0	0.3915701026366958	7.042627745264313E-4
58-59	6.402388859331194E-5	0.0	0.0	0.4484233157075568	7.042627745264313E-4
60-61	6.402388859331194E-5	0.0	0.0	0.5088618665396433	7.042627745264313E-4
62-63	6.402388859331194E-5	0.0	0.0	0.5722775281913188	7.682866631197432E-4
64-65	6.402388859331194E-5	0.0	0.0	0.6355011181772143	7.682866631197432E-4
66-67	6.402388859331194E-5	0.0	0.0	0.706887753958757	7.682866631197432E-4
68-69	6.402388859331194E-5	0.0	0.0	0.7876218774749235	7.682866631197432E-4
70-71	6.402388859331194E-5	0.0	0.0	0.8800723726036659	7.682866631197432E-4
72-73	6.402388859331194E-5	0.0	0.0	0.9764283249366004	9.60358328899679E-4
74-75	6.402388859331194E-5	0.0	0.0	1.0745129222615541	9.60358328899679E-4
76-77	6.402388859331194E-5	0.0	0.0	1.1859785123025102	9.60358328899679E-4
78-79	6.402388859331194E-5	0.0	0.0	1.315274755316704	9.92370273196335E-4
80-81	6.402388859331194E-5	0.0	0.0	1.4496929094183622	0.0010563941617896468
82-83	6.402388859331194E-5	0.0	0.0	1.5955713395782234	0.0010884061060863028
84-85	6.402388859331194E-5	0.0	0.0	1.7475960630430425	0.0011204180503829589
86-87	6.402388859331194E-5	0.0	0.0	1.9107929550673948	0.0011524299946796149
88-89	6.402388859331194E-5	0.0	0.0	2.0882991861923523	0.0012484658275695828
90-91	6.402388859331194E-5	0.0	0.0	2.2857808705584226	0.0012804777718662388
92-93	6.402388859331194E-5	0.0	0.0	2.5027898409454536	0.0013445016604595507
94-95	6.402388859331194E-5	0.0	0.0	2.724664626865576	0.0014725494376461746
96-97	6.402388859331194E-5	0.0	0.0	2.9594082143929543	0.0014725494376461746
98-99	6.402388859331194E-5	0.0	0.0	3.213743111829886	0.0014725494376461746
100-101	6.402388859331194E-5	0.0	0.0	3.5072926410302214	0.0014725494376461746
102-103	6.402388859331194E-5	0.0	0.0	3.824531009010082	0.0015365733262394864
104-105	1.6005972148327985E-4	0.0	0.0	4.155214393594537	0.0015365733262394864
106-107	1.920716657799358E-4	0.0	0.0	4.4996629142265565	0.0015685852705361425
108-109	1.920716657799358E-4	0.0	0.0	4.850929978993762	0.0016966330477227664
110-111	1.920716657799358E-4	0.0	0.0	5.2373461585986965	0.0017926688806127343
112-113	1.920716657799358E-4	0.0	0.0	5.653565458343817	0.0017926688806127343
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1810	0.0	67.071465	1
GTATCAA	3685	0.0	62.49707	1
TCAACGC	4745	0.0	48.52322	4
CAACGCA	4915	0.0	46.965946	5
ATCAACG	5070	0.0	45.175175	3
AACGCAG	5125	0.0	44.92396	6
TATCAAC	5480	0.0	41.795277	2
ACGCAGA	5985	0.0	37.97171	7
GTACATG	8675	0.0	36.700375	1
CGCAGAG	6400	0.0	35.317913	8
TACATGG	8970	0.0	34.686165	2
ACATGGG	9405	0.0	32.955353	3
GCAGAGT	6935	0.0	32.42281	9
CATGGGG	7600	0.0	30.529936	4
ATGGGGG	4060	0.0	27.695591	5
AGAGTAC	6410	0.0	27.330431	10-11
GTATAGA	465	0.0	26.875252	1
CAGAGTA	6785	0.0	25.600721	10-11
ATGGGGA	2855	0.0	24.381176	5
GAGTACT	2495	0.0	21.577654	12-13
>>END_MODULE