FastQCFastQC Report
Mon 15 Aug 2016
SRR2921518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561917
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.17958700750423998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.16671820589698427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.079.672411
GTATCAA50300.062.9491771
TCAACGC65800.047.5988664
CAACGCA66600.047.0863345
AACGCAG69600.045.2161566
TATCAAC69450.044.9257972
ATCAACG70300.044.6366963
ACGCAGA81200.038.4624147
CGCAGAG84400.037.073428
GTACATG83800.035.5074961
GCAGAGT90050.034.2838139
TACATGG86900.033.7803272
ACATGGG91200.032.2529073
CATGGGG73700.031.266574
ATGGGGG39250.028.350485
GAGTACT46250.028.16682412-13
CAGAGTA88800.027.967210-11
AGAGTAC84950.027.62416310-11
ATGGGGA29050.026.4242125
GGTATAC1859.088071E-725.7858681
Produced by FastQC (version 0.11.5)