##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921518_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561917
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315099329861958	33.0	31.0	34.0	28.0	34.0
2	31.367091849310814	33.0	31.0	34.0	28.0	34.0
3	31.450054644388914	33.0	31.0	34.0	28.0	34.0
4	34.96147554575563	37.0	35.0	37.0	32.0	37.0
5	34.62465931288282	37.0	35.0	37.0	31.0	37.0
6	34.603360485864485	37.0	35.0	37.0	31.0	37.0
7	34.446036505140796	37.0	35.0	37.0	30.0	37.0
8	34.414612940380316	37.0	35.0	37.0	30.0	37.0
9	35.996811610348054	39.0	37.0	39.0	30.0	39.0
10-11	35.86930355454227	39.0	37.0	39.0	30.0	39.0
12-13	35.780204069742496	39.0	37.0	39.0	30.0	39.0
14-15	36.954390022005015	40.0	37.0	41.0	30.0	41.0
16-17	36.92580271550921	40.0	37.0	41.0	30.0	41.0
18-19	36.957865238677854	40.0	37.0	41.0	30.0	41.0
20-21	36.94726384308513	40.0	37.0	41.0	30.0	41.0
22-23	36.88671389068689	40.0	37.0	41.0	30.0	41.0
24-25	36.82084579398265	40.0	37.0	41.0	30.0	41.0
26-27	36.69453274405746	40.0	36.5	41.0	30.0	41.0
28-29	36.51245072561474	40.0	36.0	41.0	29.5	41.0
30-31	36.46004877339833	40.0	36.0	41.0	30.0	41.0
32-33	36.33608411970675	40.0	35.5	41.0	29.5	41.0
34-35	36.21320050937406	40.0	35.0	41.0	29.0	41.0
36-37	36.079181544217775	40.0	35.0	41.0	28.0	41.0
38-39	35.90939243250442	40.0	35.0	41.0	27.5	41.0
40-41	35.715650703590526	40.0	35.0	41.0	27.0	41.0
42-43	35.542238480021666	39.5	35.0	41.0	26.5	41.0
44-45	35.32183848437529	39.0	35.0	41.0	25.5	41.0
46-47	35.041548622622074	39.0	35.0	41.0	24.0	41.0
48-49	34.777831024311794	39.0	34.5	41.0	23.0	41.0
50-51	34.55802709106822	39.0	34.0	41.0	23.0	41.0
52-53	34.36673683684857	38.5	34.0	41.0	22.5	41.0
54-55	34.150979533483536	38.0	34.0	40.5	22.0	41.0
56-57	33.8940026902838	38.0	33.5	40.0	21.0	41.0
58-59	33.75266739525852	37.0	33.0	40.0	21.5	41.0
60-61	33.63149546358737	37.0	33.0	40.0	21.0	41.0
62-63	33.359502457556964	36.5	33.0	40.0	20.0	41.0
64-65	33.059749973910264	36.0	33.0	39.5	20.0	41.0
66-67	32.756187108533936	35.5	33.0	39.0	19.0	41.0
68-69	32.438417342278754	35.0	33.0	39.0	18.0	41.0
70-71	32.129716879962245	35.0	32.0	37.5	18.0	40.0
72-73	31.811190991582777	35.0	32.0	37.0	17.0	39.5
74-75	31.517636020351915	35.0	32.0	36.5	15.0	39.0
76-77	31.222535192330962	35.0	32.0	36.0	13.0	39.0
78-79	30.946065315890664	35.0	32.0	36.0	11.0	37.5
80-81	30.722150408760516	35.0	31.5	35.0	10.0	37.0
82-83	30.53179458319488	35.0	31.0	35.0	7.5	36.5
84-85	30.339422965496887	35.0	31.0	35.0	7.0	36.0
86-87	30.196870576349447	35.0	31.0	35.0	7.0	36.0
88-89	30.091779524776285	35.0	31.0	35.0	7.0	36.0
90-91	29.993402978519345	35.0	31.0	35.0	5.0	35.0
92-93	30.052472698613308	35.0	31.0	35.0	2.0	35.0
94-95	30.021254970654653	35.0	31.0	35.0	2.0	35.0
96-97	30.002038200493367	35.0	31.0	35.0	2.0	35.0
98-99	29.964808629395797	35.0	31.0	35.0	2.0	35.0
100-101	29.914535471475116	35.0	31.0	35.0	2.0	35.0
102-103	29.034157064683974	33.5	29.5	35.0	2.0	35.0
104-105	29.73458608876144	34.5	31.0	35.0	2.0	35.0
106-107	29.83752113588622	35.0	31.0	35.0	2.0	35.0
108-109	29.845514518377097	35.0	31.0	35.0	2.0	35.0
110-111	29.81445556966215	35.0	31.5	35.0	2.0	35.0
112-113	29.768450884393985	35.0	31.0	35.0	2.0	35.0
114-115	29.70347271974119	35.0	31.0	35.0	2.0	35.0
116-117	29.630071892424503	35.0	31.0	35.0	2.0	35.0
118-119	29.535515331480482	35.0	31.0	35.0	2.0	35.0
120-121	29.411453361478237	35.0	31.0	35.0	2.0	35.0
122-123	29.29321532450188	35.0	31.0	35.0	2.0	35.0
124-125	28.376996985115085	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24636.0
3	9348.0
4	5380.0
5	4492.0
6	4863.0
7	5572.0
8	6410.0
9	7058.0
10	8261.0
11	8959.0
12	9484.0
13	11036.0
14	14336.0
15	11771.0
16	6727.0
17	5565.0
18	4957.0
19	4521.0
20	4289.0
21	4530.0
22	5336.0
23	7180.0
24	10041.0
25	14027.0
26	17714.0
27	19239.0
28	23063.0
29	27231.0
30	31884.0
31	38534.0
32	47582.0
33	61217.0
34	86086.0
35	136103.0
36	271996.0
37	455512.0
38	146706.0
39	271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.82602784246833	17.742281846497832	12.67456030195046	24.757130009083387
2	16.80155605651333	19.819808860635497	37.93630437858578	25.44233070426539
3	19.016901763821018	24.668326979615806	29.296283891741105	27.018487364822068
4	13.274602164879044	15.8503687674206	35.65586661464909	35.21916245305126
5	14.112525168687847	37.479058114692364	33.37777880245718	15.030637914162606
6	33.12336657237802	35.3656092120675	16.9072126656322	14.603811549922288
7	29.20864737485832	31.820725780114365	20.554676844066776	18.415950000960535
8	27.906912715624188	33.53227007503507	19.27144460600985	19.289372603330893
9	28.15617340705891	14.188502191022314	18.85879879218454	38.79652560973423
10-11	26.404755356963918	24.981215289905624	26.798217797628404	21.815811555502055
12-13	26.792072526153966	22.776984893799433	27.171856481810515	23.259086098236086
14-15	24.366581254697977	23.599410183616524	24.824085919062234	27.209922642623262
16-17	23.95203982807335	26.644052602511664	25.340771748961476	24.063135820453507
18-19	23.941912008108577	25.495661104505494	27.523989901415224	23.0384369859707
20-21	25.07430354137258	24.40857326634942	27.39694755961332	23.12017563266468
22-23	24.90561797177247	24.084769660468016	27.13909119199251	23.870521175767
24-25	24.33752931187062	24.619570019991624	27.17647443031555	23.866426237822207
26-27	23.92867959540026	24.713540849603103	27.339808585418453	24.01797096957818
28-29	24.059324660421964	24.739377220416433	27.360429137498688	23.840868981662915
30-31	24.497885877404904	24.587029645746377	27.157757893338076	23.757326583510643
32-33	24.08904089194574	24.747312883144875	27.07857545586776	24.08507076904162
34-35	24.178273872419997	24.87483429713166	27.215295255294485	23.731596575153855
36-37	24.44039814400959	24.627668498494536	27.003751170239603	23.928182187256265
38-39	24.347535125581157	24.92885228684504	26.914007966494612	23.809604621079192
40-41	24.38061496525809	24.795859464869256	26.917736446845343	23.905789123027308
42-43	24.3361034176183	24.778108853203033	26.699388600767538	24.186399128411125
44-45	24.294815331380843	24.857893646526037	26.62877590821433	24.218515113878794
46-47	24.477411517678213	24.967926048851023	26.31002119266111	24.244641240809656
48-49	24.621251661036485	25.248202878596242	25.84345431549287	24.287091144874402
50-51	24.519275871309983	25.427142242857375	25.707650699254014	24.345931186578625
52-53	24.715989050380863	25.272563736959384	25.729555678441873	24.28189153421788
54-55	24.439611781792475	25.212058688674922	25.90106532035174	24.447264209180865
56-57	24.45217331162818	25.2727766414308	25.798093413271822	24.4769566336692
58-59	24.44826602467534	25.134805691215963	25.91272602226115	24.50420226184755
60-61	24.653054611519142	25.32827812241244	25.910997805395514	24.107669460672906
62-63	24.44921754550109	25.309528452410575	25.962894689402084	24.278359312686256
64-65	24.415753156449025	24.943543643733097	26.112533615265583	24.5281695845523
66-67	24.455604579468844	25.1395666095391	25.926332650214395	24.47849616077766
68-69	24.41715143319796	25.23332674774518	25.888854270967386	24.46066754808947
70-71	24.363646842047125	25.029063764185693	26.080578105564456	24.526711288202723
72-73	24.6126078704095	25.403369373384667	25.58766800119877	24.396354755007057
74-75	24.333172706708055	25.512001370395925	25.617278515361726	24.537547407534298
76-77	24.371411703082718	25.080906770555185	25.74234443296104	24.805337093401057
78-79	24.251238236076787	25.264979478280473	25.752780607335303	24.731001678307436
80-81	24.4313269677816	25.469507470237524	25.489776052973685	24.609389509007194
82-83	24.44970851431464	25.345151771373033	25.596121762866492	24.60901795144584
84-85	24.239164365470046	25.40053627886501	25.457685996718293	24.902613358946653
86-87	24.15069342681263	25.482062661822802	25.59953076790332	24.767713143461243
88-89	24.2370982966793	25.239667558522434	25.598960768165746	24.924273376632524
90-91	24.194838862117685	25.2294868925693	25.70169953649587	24.873974708817144
92-93	24.3262625234269	25.35662993277512	25.61386962125738	24.7032379225406
94-95	24.258870218489136	25.113316869118457	25.651584003032458	24.97622890935995
96-97	24.20162746125584	25.1451375441335	25.699373287521038	24.95386170708963
98-99	24.058945686236157	25.119399492342076	25.544272489825552	25.277382331596215
100-101	23.998642520174105	25.248420408940795	25.654735891734582	25.09820117915052
102-103	24.140095783232812	25.006178530726185	25.753908801044904	25.099816884996095
104-105	24.065103271298206	25.199169962422935	25.573307927678396	25.16241883860046
106-107	24.074426799743005	25.181180355724607	25.54384518191023	25.200547662622153
108-109	24.083742643621484	25.083601759767415	25.49853672906114	25.334118867549964
110-111	24.006283366780814	25.267918623549562	25.547737805489014	25.178060204180607
112-113	23.914153581656386	25.226853978808926	25.623974502838976	25.23501793669571
114-115	23.694554897762078	25.43631150193854	25.55520732659505	25.313926273704336
116-117	23.68065858060947	25.405930273283804	25.455617073562713	25.45779407254401
118-119	23.66351848887466	24.995677925404195	25.704690251320635	25.636113334400513
120-121	23.466662995760423	25.144085737856958	25.872141695273594	25.51710957110903
122-123	23.514637225752093	25.238362310712283	25.649141946900844	25.59785851663478
124-125	23.449984854471644	25.252078587681083	25.58627283596656	25.71166372188071
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	51.0
2	73.5
3	109.5
4	77.0
5	67.5
6	140.0
7	292.5
8	485.5
9	621.5
10	538.5
11	408.5
12	471.5
13	575.0
14	662.0
15	757.5
16	897.5
17	1036.0
18	1186.5
19	1388.0
20	1552.5
21	1736.5
22	2006.5
23	2309.5
24	2639.0
25	3194.5
26	4260.0
27	5397.0
28	6091.0
29	7310.0
30	9435.0
31	11541.5
32	14336.5
33	17332.0
34	20352.0
35	23173.5
36	26295.0
37	29532.5
38	31061.0
39	32617.0
40	34510.0
41	36200.0
42	37686.0
43	41033.5
44	45036.5
45	47994.0
46	51389.5
47	55057.5
48	59116.0
49	63209.0
50	64861.0
51	64921.5
52	64490.0
53	64104.5
54	64820.0
55	64161.5
56	60996.0
57	56347.0
58	52330.5
59	47841.0
60	42477.0
61	37479.0
62	32091.0
63	25281.5
64	18967.0
65	14930.0
66	11547.0
67	8838.0
68	7130.5
69	5665.0
70	4597.0
71	3725.5
72	2954.0
73	2535.0
74	1977.5
75	1419.5
76	976.5
77	549.0
78	302.0
79	156.5
80	83.0
81	43.5
82	20.5
83	8.5
84	4.0
85	2.5
86	1.5
87	2.0
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12331000943071879
2	0.0012804777718662388
3	0.023304695447965547
4	0.05800564306554061
5	0.029002821532770305
6	0.025609555437324773
7	0.01837485602628053
8	0.007298723299637561
9	0.005698126084804763
10-11	0.009059380235953639
12-13	0.00691457996807769
14-15	0.004801791644498395
16-17	0.013092885217332291
18-19	0.007298723299637561
20-21	0.015909936315438016
22-23	0.011460276058202837
24-25	0.01683828270004104
26-27	0.0112682043924229
28-29	0.01747852158597416
30-31	0.02525742405006156
32-33	0.016166031869811266
34-35	0.02477724488561172
36-37	0.017350473808787535
38-39	0.0241370059996786
40-41	0.011940455222652677
42-43	0.010980096893752997
44-45	0.0206477040713431
46-47	0.018662963524950428
48-49	0.02509736432857828
50-51	0.018470891859170495
52-53	0.025705591270214743
54-55	0.020551668238453134
56-57	0.02452114933123847
58-59	0.020327584628376538
60-61	0.023048599893592297
62-63	0.02487328071850169
64-65	0.02000746518540998
66-67	0.013284956883112228
68-69	0.02730618848504754
70-71	0.01693431853293101
72-73	0.020551668238453134
74-75	0.020775751848529724
76-77	0.0155578049281748
78-79	0.033452481790005484
80-81	0.02477724488561172
82-83	0.011300216336719557
84-85	0.014693482432165087
86-87	0.013861171880452034
88-89	0.024265053776865223
90-91	0.014501410766385153
92-93	0.009219439957436919
94-95	0.009347487734623542
96-97	0.0023368719336558856
98-99	0.0024649197108425097
100-101	0.012772765774365731
102-103	0.0036493616498187807
104-105	0.003841433315598716
106-107	4.161552758565276E-4
108-109	0.022216289341879244
110-111	9.92370273196335E-4
112-113	0.011172168559532932
114-115	0.0029130869309956934
116-117	0.0085792010715038
118-119	0.010691989395083094
120-121	0.005538066363321483
122-123	5.762149973398074E-4
124-125	0.025609555437324773
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1561917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.61808121316717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68875121042053	45.11824187807463
2	10.45923341407198	11.843634537307981
3	3.5619994812526845	6.050207277324715
4	1.755774365209684	3.976343024057557
5	1.037353694587917	2.9366487863478845
6	0.7076474121387301	2.4039383190454915
7	0.49455879094003274	1.9600678853089977
8	0.35465623061131385	1.6063964214005688
9	0.2829476574899888	1.4417958105759232
>10	1.5342743043349043	15.653743159427377
>50	0.08973082306279019	3.4411039229631473
>100	0.03250633499708161	3.055596544657538
>500	2.2651235291357936E-4	0.07823063823758437
>1k	3.39768529370369E-4	0.43405179527066284
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2805	0.17958700750423998	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2604	0.16671820589698427	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	6.402388859331194E-5
9	0.0	0.0	0.0	0.0	6.402388859331194E-5
10-11	0.0	0.0	0.0	0.0	6.402388859331194E-5
12-13	0.0	0.0	0.0	0.0	6.402388859331194E-5
14-15	0.0	0.0	0.0	0.0	1.2804777718662387E-4
16-17	0.0	0.0	0.0	0.0	2.5609555437324774E-4
18-19	0.0	0.0	0.0	0.0	3.841433315598716E-4
20-21	0.0	0.0	0.0	6.402388859331194E-5	3.841433315598716E-4
22-23	0.0	0.0	0.0	1.2804777718662387E-4	5.121911087464955E-4
24-25	0.0	0.0	0.0	4.161552758565276E-4	7.042627745264313E-4
26-27	0.0	0.0	0.0	5.762149973398074E-4	7.682866631197432E-4
28-29	0.0	0.0	0.0	0.0016005972148327983	8.002986074163991E-4
30-31	0.0	0.0	0.0	0.00563410219621145	8.323105517130552E-4
32-33	0.0	0.0	0.0	0.012772765774365731	8.963344403063671E-4
34-35	0.0	0.0	0.0	0.0222483012861759	0.0010884061060863028
36-37	0.0	0.0	0.0	0.03867042871036041	0.0010884061060863028
38-39	0.0	0.0	0.0	0.06363974526175206	0.0010884061060863028
40-41	0.0	0.0	0.0	0.08982551569641664	0.0010884061060863028
42-43	0.0	0.0	0.0	0.1171957280700575	0.0011204180503829589
44-45	0.0	0.0	0.0	0.1482473140378138	0.0011524299946796149
46-47	0.0	0.0	0.0	0.18448483498162835	0.001184441938976271
48-49	0.0	0.0	0.0	0.22184277397582586	0.0012164538832729267
50-51	0.0	0.0	0.0	0.26377842100444515	0.0012164538832729267
52-53	3.201194429665597E-5	0.0	0.0	0.3041134708182317	0.0012804777718662388
54-55	6.402388859331194E-5	0.0	0.0	0.34873812116777014	0.0014085255490528625
56-57	6.402388859331194E-5	0.0	0.0	0.3982285870504002	0.0015045613819428304
58-59	6.402388859331194E-5	0.0	0.0	0.45559399123000777	0.0015365733262394864
60-61	6.402388859331194E-5	0.0	0.0	0.5175691153883337	0.0015365733262394864
62-63	6.402388859331194E-5	0.0	0.0	0.5818170875917222	0.0016005972148327985
64-65	6.402388859331194E-5	0.0	0.0	0.6460970717394074	0.0016005972148327985
66-67	6.402388859331194E-5	0.0	0.0	0.7183480300169599	0.0016005972148327985
68-69	6.402388859331194E-5	0.0	0.0	0.8010989060238156	0.0016005972148327985
70-71	6.402388859331194E-5	0.0	0.0	0.8957262133647306	0.0017286449920194222
72-73	6.402388859331194E-5	0.0	0.0	0.9940348942997611	0.001888704713502702
74-75	6.402388859331194E-5	0.0	0.0	1.0933679574522845	0.001952728602096014
76-77	6.402388859331194E-5	0.0	0.0	1.20694633581682	0.00198474054639267
78-79	6.402388859331194E-5	0.0	0.0	1.337939211878736	0.00198474054639267
80-81	6.402388859331194E-5	0.0	0.0	1.475206429022797	0.00198474054639267
82-83	6.402388859331194E-5	0.0	0.0	1.6233577072277208	0.00198474054639267
84-85	6.402388859331194E-5	0.0	0.0	1.7776232667933058	0.00198474054639267
86-87	6.402388859331194E-5	0.0	0.0	1.943060994918424	0.00198474054639267
88-89	6.402388859331194E-5	0.0	0.0	2.1246647549133533	0.00198474054639267
90-91	6.402388859331194E-5	0.0	0.0	2.3259878725950225	0.00198474054639267
92-93	6.402388859331194E-5	0.0	0.0	2.5485989332339685	0.002048764434985982
94-95	6.402388859331194E-5	0.0	0.0	2.775851725795929	0.0022408361007659177
96-97	6.402388859331194E-5	0.0	0.0	3.0153010691349156	0.0023368719336558856
98-99	6.402388859331194E-5	0.0	0.0	3.2733493521102592	0.0024329077665458535
100-101	6.402388859331194E-5	0.0	0.0	3.5725649954511027	0.0024329077665458535
102-103	6.402388859331194E-5	0.0	0.0	3.8956615492372513	0.0024329077665458535
104-105	6.402388859331194E-5	0.0	0.0	4.232907382402522	0.0024329077665458535
106-107	6.402388859331194E-5	0.0	0.0	4.581933611068962	0.0024649197108425093
108-109	1.2804777718662387E-4	0.0	0.0	4.939378981085422	0.002528943599435822
110-111	1.2804777718662387E-4	0.0	0.0	5.3360709948095835	0.0025929674880291334
112-113	1.2804777718662387E-4	0.0	0.0	5.763462463114237	0.0026249794323257893
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2395	0.0	79.67241	1
GTATCAA	5030	0.0	62.949177	1
TCAACGC	6580	0.0	47.598866	4
CAACGCA	6660	0.0	47.086334	5
AACGCAG	6960	0.0	45.216156	6
TATCAAC	6945	0.0	44.925797	2
ATCAACG	7030	0.0	44.636696	3
ACGCAGA	8120	0.0	38.462414	7
CGCAGAG	8440	0.0	37.07342	8
GTACATG	8380	0.0	35.507496	1
GCAGAGT	9005	0.0	34.283813	9
TACATGG	8690	0.0	33.780327	2
ACATGGG	9120	0.0	32.252907	3
CATGGGG	7370	0.0	31.26657	4
ATGGGGG	3925	0.0	28.35048	5
GAGTACT	4625	0.0	28.166824	12-13
CAGAGTA	8880	0.0	27.9672	10-11
AGAGTAC	8495	0.0	27.624163	10-11
ATGGGGA	2905	0.0	26.424212	5
GGTATAC	185	9.088071E-7	25.785868	1
>>END_MODULE