FastQCFastQC Report
Mon 15 Aug 2016
SRR2921517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443300
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.12672348091179933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.10760063742811612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.075.8639451
GTATCAA36600.059.6705471
TCAACGC46050.047.1588254
CAACGCA47000.046.2040145
AACGCAG48900.044.5304346
ATCAACG48750.043.932153
TATCAAC52300.041.860142
ACGCAGA57200.037.9622047
CGCAGAG60850.035.682628
GTACATG74850.035.1403051
TACATGG75750.034.0063362
ACATGGG77650.032.8677833
GCAGAGT66200.031.8104469
CATGGGG60850.029.9199314
AGAGTAC59800.027.4556910-11
CAGAGTA63700.026.75529110-11
ATGGGGG34100.024.4263385
GAGTACT26850.024.15109312-13
CATGGGT15050.022.1386594
GTACTTT30350.021.76025814-15
Produced by FastQC (version 0.11.5)