FastQCFastQC Report
Mon 15 Aug 2016
SRR2921517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443300
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.1994041432827548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.1791727291623363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24700.079.630511
GTATCAA49750.057.505671
TCAACGC61750.045.8886384
CAACGCA63550.044.6608775
AACGCAG66250.043.10426
ATCAACG66100.042.7786753
TATCAAC67800.041.618252
ACGCAGA76900.036.9012647
GTACATG73650.035.6885031
CGCAGAG80400.035.220878
ACATGGG76500.033.9281543
TACATGG77500.033.183122
GCAGAGT86050.032.4934779
CATGGGG60050.029.8393024
GTATAGG4200.028.3820111
CAGAGTA84500.027.9857610-11
GCGCGAC1954.793219E-827.4572539
AGAGTAC80700.026.72336810-11
GAGTACT49450.026.40781412-13
ATGGGGG31200.024.6013835
Produced by FastQC (version 0.11.5)