##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921517_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1443300
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162578812443705	31.0	31.0	34.0	28.0	34.0
2	31.207082380655443	33.0	31.0	34.0	28.0	34.0
3	31.285553938890043	33.0	31.0	34.0	27.0	34.0
4	34.786190674149516	37.0	35.0	37.0	32.0	37.0
5	34.437435737545904	37.0	35.0	37.0	30.0	37.0
6	34.41001524284626	37.0	35.0	37.0	30.0	37.0
7	34.24147509180351	37.0	35.0	37.0	30.0	37.0
8	34.204615810988706	37.0	35.0	37.0	30.0	37.0
9	35.7493383219012	39.0	37.0	39.0	30.0	39.0
10-11	35.62063361740456	39.0	35.0	39.0	30.0	39.0
12-13	35.53164657382388	39.0	35.0	39.0	30.0	39.0
14-15	36.68152393819719	40.0	37.0	41.0	30.0	41.0
16-17	36.672584701725214	40.0	36.5	41.0	30.0	41.0
18-19	36.70829557264602	40.0	37.0	41.0	30.0	41.0
20-21	36.701762973740735	40.0	37.0	41.0	29.5	41.0
22-23	36.65134760618028	40.0	37.0	41.0	29.0	41.0
24-25	36.595521374627594	40.0	36.0	41.0	29.0	41.0
26-27	36.47392919005058	40.0	36.0	41.0	29.0	41.0
28-29	36.28599182429156	40.0	36.0	41.0	28.5	41.0
30-31	36.231738030901404	40.0	36.0	41.0	29.0	41.0
32-33	36.09706679138087	40.0	35.0	41.0	28.0	41.0
34-35	35.96626099909929	40.0	35.0	41.0	28.0	41.0
36-37	35.83122323841197	40.0	35.0	41.0	27.0	41.0
38-39	35.658522483198226	40.0	35.0	41.0	27.0	41.0
40-41	35.45758851243677	39.5	35.0	41.0	26.0	41.0
42-43	35.28264013025705	39.0	35.0	41.0	25.0	41.0
44-45	35.057396937573614	39.0	35.0	41.0	24.5	41.0
46-47	34.76026189981293	39.0	34.0	41.0	23.5	41.0
48-49	34.4877506408924	39.0	34.0	41.0	22.0	41.0
50-51	34.267874315804065	39.0	34.0	41.0	21.0	41.0
52-53	34.07514861775098	38.0	33.5	41.0	20.0	41.0
54-55	33.862682394512575	38.0	33.0	40.0	19.5	41.0
56-57	33.60828760479457	38.0	33.0	40.0	18.5	41.0
58-59	33.47148894893647	37.0	33.0	40.0	18.5	41.0
60-61	33.356464352525464	37.0	33.0	40.0	18.0	41.0
62-63	33.09391013649276	36.5	33.0	40.0	17.5	41.0
64-65	32.80827582623155	36.0	33.0	39.5	15.0	41.0
66-67	32.51117092773505	35.5	32.5	39.0	13.5	41.0
68-69	32.195328760479455	35.0	32.0	39.0	12.5	41.0
70-71	31.894125614910276	35.0	32.0	38.0	10.5	40.0
72-73	31.580020439271117	35.0	32.0	37.0	8.0	39.5
74-75	31.286082242084113	35.0	32.0	37.0	7.0	39.0
76-77	30.991401995427147	35.0	31.5	36.0	7.0	39.0
78-79	30.707057091387792	35.0	31.0	36.0	5.5	37.5
80-81	30.47643975611446	35.0	31.0	35.0	2.0	37.0
82-83	30.28167394166147	35.0	31.0	35.0	2.0	37.0
84-85	30.076579366729025	35.0	31.0	35.0	2.0	36.0
86-87	29.926255456246103	35.0	31.0	35.0	2.0	36.0
88-89	29.80819718700201	35.0	31.0	35.0	2.0	36.0
90-91	29.70126480981085	35.0	31.0	35.0	2.0	35.5
92-93	29.75111134206333	35.0	31.0	35.0	2.0	35.0
94-95	29.714457146816322	35.0	31.0	35.0	2.0	35.0
96-97	29.691788955865032	35.0	31.0	35.0	2.0	35.0
98-99	29.648671100949215	35.0	31.0	35.0	2.0	35.0
100-101	29.59411314349061	35.0	31.0	35.0	2.0	35.0
102-103	28.72030035335689	33.5	29.0	35.0	2.0	35.0
104-105	29.41756876602231	34.5	30.5	35.0	2.0	35.0
106-107	29.521094367075452	35.0	31.0	35.0	2.0	35.0
108-109	29.530549781750153	35.0	31.0	35.0	2.0	35.0
110-111	29.49894062218527	35.0	31.0	35.0	2.0	35.0
112-113	29.444721125199194	35.0	31.0	35.0	2.0	35.0
114-115	29.38216483059655	35.0	31.0	35.0	2.0	35.0
116-117	29.30470830735121	35.0	31.0	35.0	2.0	35.0
118-119	29.21752580891014	35.0	31.0	35.0	2.0	35.0
120-121	29.096618513129634	35.0	31.0	35.0	2.0	35.0
122-123	28.97524388554008	35.0	31.0	35.0	2.0	35.0
124-125	28.07472736090903	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26115.0
3	9427.0
4	5171.0
5	4419.0
6	4616.0
7	5502.0
8	6260.0
9	7124.0
10	8229.0
11	8950.0
12	9468.0
13	10816.0
14	13975.0
15	11778.0
16	6494.0
17	5510.0
18	4958.0
19	4543.0
20	4091.0
21	4447.0
22	5224.0
23	6944.0
24	9712.0
25	13851.0
26	17003.0
27	18793.0
28	21565.0
29	25882.0
30	30046.0
31	35713.0
32	44818.0
33	57173.0
34	79033.0
35	123633.0
36	246474.0
37	406778.0
38	138437.0
39	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88390097174665	17.9818220665849	12.529413233735315	24.60486372793314
2	16.911365286654213	20.097597680825235	37.62658736537534	25.364449667145212
3	18.897073550375865	25.032035222458543	29.276485626944375	26.794405600221218
4	13.187337305589928	16.05685942163112	35.81706822506681	34.938735047712136
5	13.894635581162124	37.466169597999524	33.6342636390666	15.004931181771747
6	32.77263286431959	35.78318688637292	16.893573405167306	14.550606844140193
7	29.07882217523785	31.906893328149806	20.815462986639947	18.198821509972397
8	27.722873125098307	33.626938302075445	19.27877389899869	19.371414673827562
9	27.66191850756569	14.504034711126323	19.167199038819977	38.66684774248801
10-11	25.99040096232899	25.209409454425014	27.00726285377526	21.792926729470736
12-13	26.52804497756215	23.069189240501288	27.3786497238618	23.02411605807477
14-15	24.055019128788732	23.99882625489046	24.946656610613523	26.999498005707284
16-17	23.658886946326373	26.998997305127602	25.498870149281654	23.843245599264367
18-19	23.60724142303511	25.66898351368524	27.87890357310887	22.84487149017078
20-21	24.73627277533968	24.618294558172444	27.687252739485384	22.958179927002494
22-23	24.422292909110375	24.348079760771736	27.568417628082642	23.66120970203525
24-25	23.849700027199383	24.903407545489667	27.679546238082946	23.567346189228005
26-27	23.61041289093641	24.9028602986446	27.747083037251407	23.739643773167582
28-29	23.762884990523577	24.820536985769774	27.870628631618338	23.545949392088314
30-31	23.948791872121753	24.84124371506686	27.70848998742147	23.50147442538992
32-33	23.58241131527768	24.953545396619926	27.647306048782617	23.81673723931977
34-35	23.80253080691561	24.998076841779174	27.70761619167546	23.491776159629758
36-37	24.077109061589816	24.815858388534814	27.318202387032436	23.788830162842928
38-39	23.970474293563306	25.110364490424452	27.299289093285545	23.619872122726697
40-41	23.947217433242233	25.00838456464014	27.461320132379107	23.58307786973851
42-43	23.980648015690573	24.999350383654754	27.181125856151034	23.83887574450364
44-45	23.923411320943003	25.128672279483048	27.041726062312932	23.90619033726101
46-47	24.130494590213807	25.23490453787266	26.625551875806032	24.009048996107506
48-49	24.24475847725734	25.49889150621401	26.17705130257549	24.07929871395316
50-51	24.20041786928245	25.673736257263336	25.981559698829205	24.14428617462501
52-53	24.36140530336107	25.659940094280127	26.042840490278834	23.93581411207997
54-55	24.24763852415079	25.578852208912433	26.155287588794646	24.01822167814213
56-57	24.130513275243963	25.695676007953157	25.99863196828162	24.175178748521258
58-59	24.174218865047948	25.436785890628112	26.18397276530869	24.205022479015252
60-61	24.245235193608647	25.62404297586298	26.218103959979196	23.912617870549177
62-63	24.03761058113788	25.70087563975065	26.333436132354315	23.928077646757153
64-65	24.12480591763666	25.288555126321157	26.366476704745672	24.22016225129651
66-67	24.091626761683315	25.726042025049278	26.090254037185517	24.092077176081887
68-69	24.28241192343371	25.565066066304365	26.03391141421845	24.11861059604347
70-71	24.105663478749566	25.380530029042593	26.356966791933488	24.15683970027435
72-73	24.374844941309853	25.64677665034421	25.880005710317793	24.09837269802814
74-75	24.090487935196077	25.85424522123716	25.877391460423745	24.177875383143025
76-77	24.167502550962954	25.5205783453129	26.02796444416726	24.283954659556887
78-79	24.100388337536916	25.682328389532422	25.94653375999867	24.270749512931992
80-81	24.105624078492113	25.841827232106507	25.69809365898621	24.354455030415174
82-83	24.28503323299685	25.760319136551864	25.718466071299883	24.236181559151408
84-85	24.10075033331208	25.769389616185244	25.678508369528764	24.45135168097391
86-87	23.962265850643337	25.854655546131372	25.843845454495057	24.33923314873023
88-89	24.12749630187126	25.628924443132327	25.773557747116254	24.47002150788016
90-91	24.015944868589962	25.605199925853935	25.865473400099436	24.513381805456667
92-93	24.075889152777847	25.909646036555262	25.769709820307206	24.24475499035968
94-95	23.95744228242074	25.466932331926362	25.98076663898454	24.594858746668358
96-97	24.112870509875357	25.613184554875424	25.799429002791936	24.474515932457287
98-99	23.974549425331958	25.647454249661184	25.72287336793998	24.65512295706688
100-101	23.911225247087373	25.632712060663305	25.86526488223722	24.590797810012106
102-103	24.027638961304415	25.46685351315865	25.926994756634752	24.578512768902183
104-105	23.92995657362996	25.66818927453538	25.793670177845453	24.608183973989213
106-107	23.887476997095536	25.60417267144092	25.84937531871494	24.658975012748595
108-109	23.944720031157697	25.581885290322404	25.726828193283076	24.746566485236823
110-111	23.996857178292082	25.672578395595064	25.655984352406207	24.67458007370665
112-113	23.876578506807398	25.679734966073887	25.813748669564063	24.62993785755466
114-115	23.697289469672825	25.847560384130368	25.752913542951372	24.70223660324543
116-117	23.677686150844217	25.86651200723961	25.632998445783624	24.822803396132546
118-119	23.791786528598653	25.436760538639863	25.743657899177048	25.02779503358444
120-121	23.4764973114098	25.686646293362415	25.864927140781933	24.97192925444585
122-123	23.55729940902444	25.738043908662945	25.75678579136647	24.94787089094615
124-125	23.521682543774663	25.725384938438957	25.73761675669008	25.015315761096304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	59.0
2	106.0
3	167.0
4	120.5
5	89.0
6	157.0
7	321.0
8	522.5
9	654.5
10	585.5
11	482.5
12	522.5
13	572.0
14	674.5
15	823.0
16	976.0
17	1129.5
18	1297.0
19	1477.5
20	1681.0
21	1906.0
22	2222.0
23	2592.0
24	2900.0
25	3585.0
26	4779.5
27	5956.0
28	6671.0
29	8019.0
30	10365.5
31	12347.0
32	14795.0
33	17823.0
34	20848.5
35	23642.0
36	26766.0
37	29720.0
38	31044.5
39	32399.5
40	33935.5
41	35111.0
42	36229.0
43	39791.5
44	43772.0
45	46303.0
46	48734.5
47	50520.0
48	53547.5
49	56621.0
50	56840.5
51	56202.0
52	56156.5
53	56226.0
54	56565.0
55	55858.5
56	53251.5
57	49114.5
58	46206.5
59	43339.0
60	38333.5
61	33315.0
62	28433.5
63	22293.5
64	16652.0
65	13090.0
66	10110.5
67	7557.5
68	5890.0
69	4713.5
70	4006.5
71	3263.5
72	2623.5
73	2401.0
74	1916.5
75	1415.5
76	934.5
77	501.5
78	320.0
79	200.0
80	92.5
81	33.5
82	19.5
83	10.0
84	4.5
85	1.5
86	2.0
87	2.0
88	2.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12277419801843
2	0.001939998614286704
3	0.024734982332155476
4	0.0550821035127832
5	0.03055497817501559
6	0.023764983025012124
7	0.018499272500519644
8	0.006304995496431788
9	0.005127139194900575
10-11	0.009318921915055775
12-13	0.006339638328829765
14-15	0.0039146400609713855
16-17	0.013094990646435252
18-19	0.007136423473983232
20-21	0.01642070255664103
22-23	0.011051063534954619
24-25	0.017494630360978314
26-27	0.010150349892607219
28-29	0.01697498787500866
30-31	0.02528926765052311
32-33	0.015554631746691609
34-35	0.025012124991339296
36-37	0.017529273193376292
38-39	0.02452712533776762
40-41	0.011917134344904039
42-43	0.009734635903831498
44-45	0.020300699785214437
46-47	0.018499272500519644
48-49	0.024319268343379753
50-51	0.018360701170927734
52-53	0.02535855331531906
54-55	0.020335342617612415
56-57	0.024319268343379753
58-59	0.019919628628836693
60-61	0.02428462551098178
62-63	0.024596411002563566
64-65	0.02019677128802051
66-67	0.01278320515485346
68-69	0.02726390909720779
70-71	0.01711355920460057
72-73	0.020647128109194206
74-75	0.02085498510358207
76-77	0.015069632093119933
78-79	0.033464976096445644
80-81	0.024423196840573688
82-83	0.010808563708168779
84-85	0.014411418277558374
86-87	0.014134275618374558
88-89	0.02359176886302224
90-91	0.014030347121180627
92-93	0.009873207233423406
94-95	0.00904177925587196
96-97	0.002459641100256357
98-99	0.002217141273470519
100-101	0.012956419316843345
102-103	0.0032910690778078016
104-105	0.00363749740178757
106-107	2.7714265918381487E-4
108-109	0.02203284140511328
110-111	7.621423127554909E-4
112-113	0.01136284902653641
114-115	0.002771426591838149
116-117	0.008937850758678029
118-119	0.010150349892607219
120-121	0.00495392503291069
122-123	5.889281507656066E-4
124-125	0.02369569736021617
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1443300.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.29003241268597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21762595495623	46.529604234408474
2	9.719779267248416	11.136929385296297
3	3.2367584445713486	5.563019886045827
4	1.6050491005885348	3.6781325998667844
5	0.9562719419857932	2.739242527585412
6	0.6371897151833348	2.1902771661530034
7	0.4728470903203392	1.896259759148754
8	0.3135521840592543	1.4370731830258523
9	0.2384048926873307	1.2292401626460085
>10	1.4640685323069456	15.37105595056262
>50	0.09947458048057203	3.856562466975862
>100	0.03837116843227465	3.7956332919774316
>500	2.4285087187871709E-4	0.09864270433858952
>1k	3.6427630781807563E-4	0.4783266819690702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2878	0.1994041432827548	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2586	0.1791727291623363	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.928566479595372E-5
2	0.0	0.0	0.0	0.0	6.928566479595372E-5
3	0.0	0.0	0.0	0.0	6.928566479595372E-5
4	0.0	0.0	0.0	0.0	6.928566479595372E-5
5	0.0	0.0	0.0	0.0	6.928566479595372E-5
6	0.0	0.0	0.0	0.0	6.928566479595372E-5
7	0.0	0.0	0.0	0.0	6.928566479595372E-5
8	0.0	0.0	0.0	0.0	6.928566479595372E-5
9	0.0	0.0	0.0	0.0	1.3857132959190744E-4
10-11	0.0	0.0	0.0	0.0	1.3857132959190744E-4
12-13	0.0	0.0	0.0	0.0	2.7714265918381487E-4
14-15	0.0	0.0	0.0	0.0	3.1178549158179174E-4
16-17	0.0	0.0	0.0	0.0	4.84999653571676E-4
18-19	0.0	0.0	0.0	3.464283239797686E-5	4.84999653571676E-4
20-21	0.0	0.0	0.0	6.928566479595372E-5	5.542853183676297E-4
22-23	0.0	0.0	0.0	1.0392849719393057E-4	5.542853183676297E-4
24-25	0.0	0.0	0.0	2.0785699438786114E-4	5.542853183676297E-4
26-27	0.0	0.0	0.0	5.196424859696528E-4	5.542853183676297E-4
28-29	0.0	0.0	0.0	0.0012817847987251438	5.889281507656067E-4
30-31	0.0	0.0	0.0	0.004226425552553177	6.582138155615603E-4
32-33	0.0	0.0	0.0	0.010323564054597105	6.928566479595372E-4
34-35	0.0	0.0	0.0	0.018533915332917622	6.928566479595372E-4
36-37	0.0	0.0	0.0	0.029758193029862122	6.928566479595372E-4
38-39	0.0	0.0	0.0	0.048846393681147376	6.928566479595372E-4
40-41	0.0	0.0	0.0	0.07074066375666874	6.928566479595372E-4
42-43	0.0	0.0	0.0	0.09173422018984273	7.274994803575141E-4
44-45	0.0	0.0	0.0	0.11636527402480426	8.314279775514446E-4
46-47	0.0	0.0	0.0	0.14380239728400193	9.007136423473983E-4
48-49	0.0	0.0	0.0	0.17636665973810017	9.007136423473983E-4
50-51	0.0	0.0	0.0	0.20737199473428947	9.007136423473983E-4
52-53	0.0	0.0	0.0	0.24017875701517355	9.69999307143352E-4
54-55	0.0	0.0	0.0	0.27468301808355855	0.001004642139541329
56-57	0.0	0.0	0.0	0.31414120418485414	0.0010739278043372827
58-59	0.0	0.0	0.0	0.36187902722926624	0.0011085706367352595
60-61	0.0	0.0	0.0	0.4120418485415368	0.0011085706367352595
62-63	0.0	0.0	0.0	0.46532252476962516	0.001177856301531213
64-65	0.0	0.0	0.0	0.5212014134275618	0.001177856301531213
66-67	0.0	0.0	0.0	0.5810988706436638	0.00121249913392919
68-69	0.0	0.0	0.0	0.6518395344003325	0.001247141966327167
70-71	0.0	0.0	0.0	0.7308598351001179	0.0012817847987251438
72-73	0.0	0.0	0.0	0.8119587057437816	0.0013164276311231206
74-75	0.0	0.0	0.0	0.8916718630915264	0.0013164276311231206
76-77	0.0	0.0	0.0	0.9818471558234602	0.0013857132959190742
78-79	0.0	0.0	0.0	1.0876117231344835	0.001454998960715028
80-81	0.0	0.0	0.0	1.1998545001039285	0.001454998960715028
82-83	0.0	0.0	0.0	1.3214854846532251	0.0015242846255109819
84-85	0.0	0.0	0.0	1.4427353980461444	0.0015935702903069355
86-87	0.0	0.0	0.0	1.5723688768793735	0.0016974987875008661
88-89	0.0	0.0	0.0	1.723238411972563	0.0018014272846947966
90-91	0.0	0.0	0.0	1.896902930783621	0.0018014272846947966
92-93	0.0	0.0	0.0	2.0892745790895866	0.0018707129494907504
94-95	0.0	0.0	0.0	2.2811958705743782	0.0018707129494907504
96-97	0.0	0.0	0.0	2.4780710870920806	0.0020785699438786113
98-99	0.0	0.0	0.0	2.6941384327582623	0.002217141273470519
100-101	0.0	0.0	0.0	2.946684680939514	0.002217141273470519
102-103	0.0	0.0	0.0	3.217660915956489	0.0022864269382664726
104-105	0.0	0.0	0.0	3.498302501212499	0.002355712603062426
106-107	0.0	0.0	0.0	3.7863922954340747	0.0024596411002563573
108-109	0.0	0.0	0.0	4.091526363195455	0.002494283932654334
110-111	0.0	0.0	0.0	4.424547911037206	0.0025982124298482645
112-113	0.0	0.0	0.0	4.790999792143006	0.0027021409270421948
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2470	0.0	79.63051	1
GTATCAA	4975	0.0	57.50567	1
TCAACGC	6175	0.0	45.888638	4
CAACGCA	6355	0.0	44.660877	5
AACGCAG	6625	0.0	43.1042	6
ATCAACG	6610	0.0	42.778675	3
TATCAAC	6780	0.0	41.61825	2
ACGCAGA	7690	0.0	36.901264	7
GTACATG	7365	0.0	35.688503	1
CGCAGAG	8040	0.0	35.22087	8
ACATGGG	7650	0.0	33.928154	3
TACATGG	7750	0.0	33.18312	2
GCAGAGT	8605	0.0	32.493477	9
CATGGGG	6005	0.0	29.839302	4
GTATAGG	420	0.0	28.382011	1
CAGAGTA	8450	0.0	27.98576	10-11
GCGCGAC	195	4.793219E-8	27.457253	9
AGAGTAC	8070	0.0	26.723368	10-11
GAGTACT	4945	0.0	26.407814	12-13
ATGGGGG	3120	0.0	24.601383	5
>>END_MODULE