FastQCFastQC Report
Mon 15 Aug 2016
SRR2921516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503623
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.075.492911
GTATCAA31450.059.231721
TCAACGC40200.044.9903344
CAACGCA41200.043.898335
AACGCAG43100.041.9617466
TATCAAC44650.041.8346862
ATCAACG44950.040.762783
GTACATG74950.036.447831
TACATGG77050.034.6658632
ACGCAGA52250.034.4984137
ACATGGG77900.033.9069143
CGCAGAG55150.032.2528728
CATGGGG67300.031.8243184
ATGGGGG36500.029.3394055
GCAGAGT61250.028.555129
TACCGTA1306.386977E-527.4568487
AGAGTAC54350.025.17467310-11
CAGAGTA59300.023.82563410-11
TCTATAC4600.023.2786313
GTATAGA6000.022.8143061
Produced by FastQC (version 0.11.5)