##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921516_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1503623
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.771390168945274	31.0	30.0	34.0	25.0	34.0
2	29.794603434504527	31.0	30.0	34.0	25.0	34.0
3	29.94776815731071	31.0	30.0	34.0	25.0	34.0
4	33.32423153942178	37.0	35.0	37.0	27.0	37.0
5	33.34809190867658	37.0	35.0	37.0	27.0	37.0
6	33.3460315517919	37.0	35.0	37.0	27.0	37.0
7	33.213802927994585	37.0	35.0	37.0	26.0	37.0
8	33.129963428332765	37.0	35.0	37.0	26.0	37.0
9	34.59629973736768	39.0	35.0	39.0	26.0	39.0
10-11	34.480713583125564	39.0	35.0	39.0	25.0	39.0
12-13	34.31880930259779	39.0	35.0	39.0	24.5	39.0
14-15	35.4636877062934	40.0	36.0	41.0	25.0	41.0
16-17	35.388350670347556	40.0	36.0	41.0	24.5	41.0
18-19	35.39623263278096	40.0	36.0	41.0	24.0	41.0
20-21	35.41914329589265	40.0	36.0	41.0	24.0	41.0
22-23	35.383511358897806	40.0	35.0	41.0	24.0	41.0
24-25	35.33703794102644	40.0	35.0	41.0	24.0	41.0
26-27	35.25698462979085	40.0	35.0	41.0	24.0	41.0
28-29	35.220524027631924	40.0	35.0	41.0	24.0	41.0
30-31	35.1274268882559	40.0	35.0	41.0	24.0	41.0
32-33	35.01882619513003	40.0	35.0	41.0	24.0	41.0
34-35	34.84978847756386	39.5	34.5	41.0	23.0	41.0
36-37	34.710322002257215	39.0	34.0	41.0	22.0	41.0
38-39	34.58570865170326	39.0	34.0	41.0	21.5	41.0
40-41	34.43448623757418	39.0	34.0	41.0	20.5	41.0
42-43	34.2365948778384	39.0	33.5	41.0	18.5	41.0
44-45	34.040424029161564	39.0	33.0	41.0	17.5	41.0
46-47	33.78769844568752	38.0	33.0	41.0	15.0	41.0
48-49	33.64049765133946	38.0	33.0	40.5	14.0	41.0
50-51	33.44229570843223	38.0	33.0	40.0	12.0	41.0
52-53	33.24180329776813	38.0	33.0	40.0	9.0	41.0
54-55	33.03337372466369	37.5	32.5	40.0	8.0	41.0
56-57	32.788952084398815	37.0	32.0	40.0	7.5	41.0
58-59	32.53422367175815	37.0	32.0	40.0	6.5	41.0
60-61	32.21399579548863	36.0	31.5	40.0	3.5	41.0
62-63	31.894499485575842	35.5	31.0	39.0	2.0	41.0
64-65	31.58308000077147	35.0	31.0	39.0	2.0	41.0
66-67	31.53652444795005	35.0	31.0	39.0	2.0	41.0
68-69	31.29008434960093	35.0	31.0	38.0	2.0	40.0
70-71	30.997152876751684	35.0	31.0	37.0	2.0	40.0
72-73	30.675055183380408	35.0	31.0	37.0	2.0	39.0
74-75	30.377243830401635	35.0	31.0	36.0	2.0	39.0
76-77	30.10604519882976	35.0	30.0	36.0	2.0	38.5
78-79	29.839135873819433	35.0	30.0	35.0	2.0	37.0
80-81	29.610649078924702	35.0	30.0	35.0	2.0	37.0
82-83	29.422953759020714	35.0	30.0	35.0	2.0	36.0
84-85	29.235618569282327	34.5	30.0	35.0	2.0	36.0
86-87	29.083732092419442	34.0	30.0	35.0	2.0	36.0
88-89	28.9620220627112	34.0	30.0	35.0	2.0	35.0
90-91	28.855146536066556	34.0	29.0	35.0	2.0	35.0
92-93	28.75496683676693	34.0	29.0	35.0	2.0	35.0
94-95	28.695710294402254	34.0	29.0	35.0	2.0	35.0
96-97	28.599599434166677	34.0	29.0	35.0	2.0	35.0
98-99	28.54009814960266	34.0	29.0	35.0	2.0	35.0
100-101	28.471507818116642	34.0	29.0	35.0	2.0	35.0
102-103	27.487866971973695	32.5	26.5	34.5	2.0	35.0
104-105	28.309421643590184	34.0	29.0	35.0	2.0	35.0
106-107	28.633463640819542	34.0	29.5	35.0	2.0	35.0
108-109	28.674084195306936	35.0	30.0	35.0	2.0	35.0
110-111	28.61574277594849	35.0	30.0	35.0	2.0	35.0
112-113	28.536343884072004	35.0	30.0	35.0	2.0	35.0
114-115	28.413208962618953	34.5	29.0	35.0	2.0	35.0
116-117	28.351320444020875	34.0	29.0	35.0	2.0	35.0
118-119	28.19646048244806	34.0	29.0	35.0	2.0	35.0
120-121	28.022408875097014	34.0	29.0	35.0	2.0	35.0
122-123	27.893716044513816	34.0	29.0	35.0	2.0	35.0
124-125	27.03859810604121	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61007.0
3	10685.0
4	7011.0
5	6023.0
6	6222.0
7	6944.0
8	7884.0
9	8266.0
10	8700.0
11	9379.0
12	9035.0
13	9323.0
14	10299.0
15	8992.0
16	6672.0
17	5834.0
18	5492.0
19	5052.0
20	4864.0
21	5271.0
22	6282.0
23	8437.0
24	11853.0
25	16533.0
26	19670.0
27	20675.0
28	24776.0
29	28987.0
30	32852.0
31	39518.0
32	49134.0
33	63865.0
34	88328.0
35	137870.0
36	265085.0
37	383757.0
38	102896.0
39	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.68907348344836	16.428427916977686	11.720202193579462	25.16229640599449
2	16.97554147890032	19.06482046942585	37.97250927688834	25.98712877478549
3	19.393229798996146	23.849441692916134	28.567893268050597	28.18943524003712
4	13.366619874792928	14.75035093905303	35.0214555155644	36.86157367058964
5	14.50638454429995	37.0484530794374	33.17647794566235	15.268684430600308
6	34.26185419394675	33.981202026976675	17.068615396080187	14.68832838299639
7	30.308284343338915	30.830097642891552	20.254645954699445	18.606972059070088
8	28.24831581622538	33.44490260807317	19.016606990077854	19.2901745856236
9	27.998568348391622	14.331883719553288	18.250231678751216	39.419316253303876
10-11	26.769762696619047	24.562819299087153	26.621102962749575	22.046315041544226
12-13	26.867952972358754	22.132124200660495	26.88288861272184	24.11703421425891
14-15	24.200989339206433	23.29886845700406	24.091051672699102	28.409090531090403
16-17	24.62622023550138	26.352296700598966	24.434847923379223	24.586635140520436
18-19	24.66601480430655	25.538277173694258	25.91525500981952	23.880453012179668
20-21	25.844279171785224	24.5030950913754	25.57636178031306	24.07626395652631
22-23	25.578303448028052	24.11636670457366	25.531690489958418	24.773639357439876
24-25	24.9471175970875	24.57859621136723	25.537683516163202	24.936602675382073
26-27	24.691118978204912	24.658814433178332	25.58742870792799	25.062637880688772
28-29	24.784114500159692	24.778491962099437	25.642632811668264	24.794760726072607
30-31	25.10957185964422	24.71413505268166	25.43580888884453	24.74048419882959
32-33	24.791035748009175	24.741394053244377	25.416447903836186	25.05112229491026
34-35	24.97103365684489	24.770340061375716	25.551059105311747	24.707567176467656
36-37	25.09368055885947	24.67008440316426	25.388687400875604	24.84754763710066
38-39	25.19193490749792	24.761549533746404	25.31041981789059	24.736095740865082
40-41	25.13969584299239	24.697819161252717	25.38605157468055	24.776433421074344
42-43	24.927017863737845	24.763752150203125	25.36038568904652	24.948844297012506
44-45	24.86193617421961	24.785409653121913	25.383780503304948	24.968873669353524
46-47	24.990708246252602	24.79133066897632	25.272771617843226	24.945189466927843
48-49	25.080834820170594	24.933454121186855	25.00830887800704	24.977402180635508
50-51	25.046203187562483	25.13423460739351	24.934317448752306	24.8852447562917
52-53	25.155917270017035	25.00502422274276	25.06165486584327	24.777403641396933
54-55	24.849410717339275	24.904602945315446	25.275212601526754	24.970773735818526
56-57	24.87237047924028	24.958565732280405	25.104043092969114	25.065020695510192
58-59	25.09225190458764	24.89607106024818	25.019395189460724	24.992281845703452
60-61	25.04894008336696	24.930020254624818	25.22776889436493	24.793270767643293
62-63	24.939361710267573	25.078586092399078	25.087601578075937	24.89445061925741
64-65	24.926678997724522	24.787921329625146	25.270495948049877	25.01490372460046
66-67	24.83672175470974	25.0272934298178	25.19800626568	24.937978549792465
68-69	24.87799117864247	25.008714915811254	25.143097587099263	24.970196318447016
70-71	24.75845519115484	24.838301965195456	25.216276631810224	25.18696621183948
72-73	24.924933315637322	25.12747282647563	25.01757127551328	24.930022582373773
74-75	24.821710501366237	25.153872203737375	25.067912680559907	24.956504614336477
76-77	24.857258708590887	24.834672903320445	25.158890641127517	25.149177746961143
78-79	24.82143338275176	24.958844773028382	25.138800140105733	25.08092170411412
80-81	24.810753655957647	25.106243707448883	24.988799383741593	25.094203252851877
82-83	24.899716202090186	25.11127585336432	25.020978030190406	24.968029914355093
84-85	24.837803850668124	25.14897567066075	24.920652183673855	25.09256829499727
86-87	24.61896781493212	25.234579200849282	25.055982056474612	25.090470927743986
88-89	24.750608408054635	25.069505028409004	25.02121154772956	25.158675015806804
90-91	24.64977079292611	25.05254602100245	25.05933044396732	25.23835274210413
92-93	24.771715657237735	25.17006372837431	24.992558204816167	25.065662409571793
94-95	24.696367181614978	24.979787743873217	25.102275180014626	25.221569894497176
96-97	24.622833646391495	25.000565367076216	25.103561945607694	25.273039040924594
98-99	24.594366745206546	25.059320610648943	25.010999462913603	25.335313181230905
100-101	24.594082718231565	25.084745199046875	25.101340059960993	25.21983202276057
102-103	24.54237060423874	24.922441191151663	25.271983752971742	25.263204451637854
104-105	24.53625231412431	25.078905528524874	25.08139964490434	25.303442512446473
106-107	24.480444935743183	25.04745599927635	25.1247424339773	25.347356631003166
108-109	24.483842913846058	24.993539826099102	25.224077409012978	25.298539851041856
110-111	24.523506904053573	25.103106873991127	25.190334524524506	25.18305169743079
112-113	24.506243271942076	25.164807281151262	25.09633365114764	25.232615795759024
114-115	24.36086694958402	25.285109459485078	25.16239591380521	25.191627677125695
116-117	24.282055272665357	25.28742397880186	25.106748926524812	25.323771822007973
118-119	24.48175669572249	25.060573243375945	25.094105604270812	25.363564456630755
120-121	24.24000838280031	25.123572888315206	25.289126520216833	25.347292208667653
122-123	24.165914669091684	25.226472399055762	25.204951908188995	25.40266102366356
124-125	24.188131165334724	25.21472257988997	25.174917263469293	25.42222899130601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	138.5
2	169.5
3	178.0
4	172.0
5	167.5
6	155.0
7	184.0
8	242.5
9	306.5
10	353.0
11	382.0
12	439.0
13	512.5
14	545.0
15	578.0
16	663.0
17	749.0
18	820.0
19	924.5
20	1061.0
21	1189.0
22	1412.5
23	1721.0
24	2021.5
25	2552.0
26	3300.0
27	4111.0
28	4969.5
29	6018.5
30	7350.0
31	8635.5
32	9937.0
33	11347.5
34	12980.0
35	14950.0
36	17425.5
37	20000.0
38	22565.0
39	25572.5
40	28673.0
41	31284.0
42	34296.5
43	38902.5
44	43447.5
45	47001.0
46	50936.0
47	54839.0
48	59276.5
49	63389.5
50	64433.5
51	65172.0
52	65989.0
53	66609.5
54	67488.5
55	67236.0
56	63907.0
57	58538.0
58	54873.0
59	50903.0
60	45129.0
61	39558.5
62	34148.0
63	27012.0
64	20602.5
65	16337.5
66	12534.0
67	9713.0
68	7561.0
69	5860.5
70	4840.0
71	3945.5
72	3181.0
73	2687.0
74	2150.0
75	1553.0
76	1039.5
77	626.5
78	375.5
79	237.5
80	138.0
81	67.0
82	43.5
83	33.5
84	27.5
85	21.5
86	18.0
87	16.5
88	14.5
89	11.0
90	7.0
91	6.0
92	3.5
93	3.0
94	6.5
95	8.0
96	5.5
97	3.5
98	3.0
99	3.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03385157050670281
2	0.0282650637826104
3	0.027799521555602703
4	0.03411759463642149
5	0.03591325751202262
6	0.04622169253862171
7	0.03957108929565457
8	0.034649642895858875
9	0.030858799047367592
10-11	0.03498217305800722
12-13	0.03371855844184347
14-15	0.03315325716619126
16-17	0.035381209252585255
18-19	0.04984627130603882
20-21	0.06125205586772748
22-23	0.05466795865719
24-25	0.06627326131616768
26-27	0.04911470494931243
28-29	0.04941398209524595
30-31	0.048416391608800874
32-33	0.05679615169493949
34-35	0.03893928198757268
36-37	0.05164193418163995
38-39	0.05985542918670438
40-41	0.047551813187215144
42-43	0.057062175824658175
44-45	0.05825928440839226
46-47	0.06294795969468411
48-49	0.04738554810614097
50-51	0.04991277733846849
52-53	0.06005494728399339
54-55	0.04615518650619205
56-57	0.04256386075498978
58-59	0.04475855982516894
60-61	0.03388482352291765
62-63	0.04346169219279035
64-65	0.042763378852278794
66-67	0.03418410066885117
68-69	0.030127232690641204
70-71	0.049513741143890455
72-73	0.030559521901434072
74-75	0.039238559133506205
76-77	0.03102506412844177
78-79	0.0314241003230198
80-81	0.023775906593607572
82-83	0.01759084557764812
84-85	0.018189399869515163
86-87	0.015928194766906333
88-89	0.020916147199131697
90-91	0.011904579804911204
92-93	0.01925349638838991
94-95	0.013533977599438158
96-97	0.011638555675192518
98-99	0.009344097556368851
100-101	0.00967662771851721
102-103	0.005752771805166587
104-105	0.0056197597403072445
106-107	0.008313254053708941
108-109	0.012004338853555712
110-111	0.005719518788951752
112-113	0.008280001037494105
114-115	0.00808048294020509
116-117	0.006318073080818796
118-119	0.23805834308200927
120-121	0.35361257442856353
122-123	0.02690169011780213
124-125	0.0036578317836319344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1503623.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.70104015372644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83001548681136	49.87296031178078
2	10.174428129682559	12.555455971414947
3	3.429048364218072	6.3472755242906755
4	1.643933701347569	4.05729677267642
5	0.9688432800025154	2.988931906105162
6	0.6333999042370299	2.3448859754817275
7	0.4520802326808034	1.952567441254097
8	0.32798910955723826	1.618981537502091
9	0.24850322793699195	1.3799616880743877
>10	1.2260082602517925	13.098362896615603
>50	0.05089334443461402	2.1026147564715867
>100	0.014423209947593855	1.4351569156039385
>500	3.253116688621683E-4	0.16030891916561738
>1k	1.084372229540561E-4	0.08523938356302026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.650603242967153E-5
2	0.0	0.0	0.0	0.0	6.650603242967153E-5
3	0.0	0.0	0.0	0.0	6.650603242967153E-5
4	0.0	0.0	0.0	0.0	6.650603242967153E-5
5	0.0	0.0	0.0	0.0	6.650603242967153E-5
6	0.0	0.0	0.0	0.0	6.650603242967153E-5
7	0.0	0.0	0.0	0.0	6.650603242967153E-5
8	0.0	0.0	0.0	0.0	6.650603242967153E-5
9	0.0	0.0	0.0	0.0	6.650603242967153E-5
10-11	0.0	0.0	0.0	0.0	1.3301206485934305E-4
12-13	0.0	0.0	0.0	0.0	4.3228921079286497E-4
14-15	0.0	0.0	0.0	0.0	4.655422270077007E-4
16-17	0.0	0.0	0.0	0.0	5.320482594373722E-4
18-19	0.0	0.0	0.0	3.3253016214835764E-5	5.320482594373722E-4
20-21	0.0	0.0	0.0	6.650603242967153E-5	6.650603242967153E-4
22-23	0.0	0.0	0.0	1.995180972890146E-4	7.315663567263868E-4
24-25	0.0	0.0	0.0	2.660241297186861E-4	7.980723891560584E-4
26-27	0.0	0.0	0.0	3.3253016214835766E-4	8.645784215857299E-4
28-29	0.0	0.0	0.0	9.310844540154014E-4	8.645784215857299E-4
30-31	0.0	0.0	0.0	0.003657831783631934	8.645784215857299E-4
32-33	0.0	0.0	0.0	0.008379760086138614	9.310844540154014E-4
34-35	0.0	0.0	0.0	0.014232290939949708	9.310844540154014E-4
36-37	0.0	0.0	0.0	0.02397542469089659	9.310844540154014E-4
38-39	0.0	0.0	0.0	0.04066843883074414	9.310844540154014E-4
40-41	0.0	0.0	0.0	0.06028771839749725	9.310844540154014E-4
42-43	0.0	0.0	0.0	0.07774555191028602	9.310844540154014E-4
44-45	0.0	0.0	0.0	0.0969657952824611	9.310844540154014E-4
46-47	0.0	0.0	0.0	0.11997688250312744	9.310844540154014E-4
48-49	0.0	0.0	0.0	0.1464130303939219	0.0010640965188747444
50-51	0.0	0.0	0.0	0.17447857607924327	0.0010640965188747444
52-53	0.0	0.0	0.0	0.20354171225100973	0.001130602551304416
54-55	0.0	0.0	0.0	0.23430075224973282	0.0011971085837340877
56-57	0.0	0.0	0.0	0.2689503951455917	0.0011971085837340877
58-59	0.0	0.0	0.0	0.31068293049521056	0.0011971085837340877
60-61	0.0	0.0	0.0	0.3557740204825278	0.0011971085837340877
62-63	0.0	0.0	0.0	0.4043566771724029	0.001263614616163759
64-65	0.0	0.0	0.0	0.4519084903596181	0.001263614616163759
66-67	0.0	0.0	0.0	0.5058116296438668	0.001263614616163759
68-69	0.0	0.0	0.0	0.5679947699656097	0.001263614616163759
70-71	0.0	0.0	0.0	0.6390564656167138	0.001263614616163759
72-73	0.0	0.0	0.0	0.7139422581325239	0.001429879697237938
74-75	0.0	0.0	0.0	0.7911557617833727	0.0014963857296676096
76-77	0.0	0.0	0.0	0.873456976915091	0.0015296387458824453
78-79	0.0	0.0	0.0	0.9682945791598027	0.0015296387458824453
80-81	0.0	0.0	6.650603242967153E-5	1.0783620628309092	0.0015296387458824453
82-83	3.3253016214835764E-5	0.0	6.650603242967153E-5	1.1931182217883074	0.0015961447783121169
84-85	6.650603242967153E-5	0.0	6.650603242967153E-5	1.3085726940862172	0.0015961447783121169
86-87	6.650603242967153E-5	0.0	6.650603242967153E-5	1.4273524680056104	0.0016293977945269526
88-89	6.650603242967153E-5	0.0	6.650603242967153E-5	1.5664165818160536	0.0017291568431714599
90-91	6.650603242967153E-5	0.0	6.650603242967153E-5	1.7308527469984165	0.0017291568431714599
92-93	6.650603242967153E-5	0.0	6.650603242967153E-5	1.9063289135641048	0.0017624098593862956
94-95	6.650603242967153E-5	0.0	6.650603242967153E-5	2.0819380921946524	0.0017956628756011315
96-97	6.650603242967153E-5	0.0	6.650603242967153E-5	2.2623357051601367	0.0018289158918159672
98-99	6.650603242967153E-5	0.0	6.650603242967153E-5	2.459858621476261	0.0018621689080308029
100-101	6.650603242967153E-5	0.0	6.650603242967153E-5	2.6887391320829757	0.0019286749404604745
102-103	6.650603242967153E-5	0.0	6.650603242967153E-5	2.9482789236397684	0.00196192795667531
104-105	6.650603242967153E-5	0.0	6.650603242967153E-5	3.2067878716939022	0.001995180972890146
106-107	6.650603242967153E-5	0.0	6.650603242967153E-5	3.480327183077141	0.001995180972890146
108-109	6.650603242967153E-5	0.0	6.650603242967153E-5	3.766269869508514	0.0020284339891049816
110-111	6.650603242967153E-5	0.0	6.650603242967153E-5	4.0822732825980985	0.0020949400215346534
112-113	6.650603242967153E-5	0.0	6.650603242967153E-5	4.420123927340829	0.002128193037749489
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1490	0.0	75.49291	1
GTATCAA	3145	0.0	59.23172	1
TCAACGC	4020	0.0	44.990334	4
CAACGCA	4120	0.0	43.89833	5
AACGCAG	4310	0.0	41.961746	6
TATCAAC	4465	0.0	41.834686	2
ATCAACG	4495	0.0	40.76278	3
GTACATG	7495	0.0	36.44783	1
TACATGG	7705	0.0	34.665863	2
ACGCAGA	5225	0.0	34.498413	7
ACATGGG	7790	0.0	33.906914	3
CGCAGAG	5515	0.0	32.252872	8
CATGGGG	6730	0.0	31.824318	4
ATGGGGG	3650	0.0	29.339405	5
GCAGAGT	6125	0.0	28.55512	9
TACCGTA	130	6.386977E-5	27.456848	7
AGAGTAC	5435	0.0	25.174673	10-11
CAGAGTA	5930	0.0	23.825634	10-11
TCTATAC	460	0.0	23.278631	3
GTATAGA	600	0.0	22.814306	1
>>END_MODULE